An EPR study of wheat seeds by the use of nitroxide spin probes

The viability of a variety of wheat seeds was investigated by use of the EPR technique. Three different types of spin probes (4-Hydroxy-TEMPO (TANOL), 3-Carbamoyl-2,2,5,5-tetramethyl-3-pyrrolin-1-yloxy, 3-Carbamoyl-PROXYL) were utilized in examining the viability of embryo cells. The penetration of the spin probe across the membrane was observed via the change in signal intensity against time for dry embryos of the variety of wheat seeds soaked in aqueous solutions of spin probe and line broadening agent. From the analysis of the signal intensity-time curves (i.e. rehydration curves) it was observed that the two kinds having different genetic roots (Bezostaya, Kunduru) exhibit similar saturation behaviour whereas Gerek saturates much more easily. The rehydration curves for naturally aged seeds indicates that aging causes rapid saturation and an important decrease in the signal intensity. In order to get better insight on the effect of the signals from polar and nonpolar regions on the experimental spectra, these spectra were simulated by the use of the theoretical models developed.

Yazar Sunnetcioglu M.M.
Dadayli D.
Sungur R.
Bingol G.
Yayın Türü Article
Tek Biçim Adres https://hdl.handle.net/20.500.12628/4202
Tek Biçim Adres 10.1016/S0176-1617(97)80152-6
Konu Başlıkları EPR
Nitroxide spin probes
Simulation of EPR spectra
Wheat seed
Koleksiyonlar Araştırma Çıktıları | WoS | Scopus | TR-Dizin | PubMed | SOBİAD
Scopus İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu
Dergi Adı Journal of Plant Physiology
Dergi Cilt Bilgisi 151
Dergi Sayısı 2
Sayfalar 196 - 200
Yayın Yılı 1997
Eser Adı
[dc.title]
An EPR study of wheat seeds by the use of nitroxide spin probes
Yazar
[dc.contributor.author]
Sunnetcioglu M.M.
Yazar
[dc.contributor.author]
Dadayli D.
Yazar
[dc.contributor.author]
Sungur R.
Yazar
[dc.contributor.author]
Bingol G.
Yayın Yılı
[dc.date.issued]
1997
Yayıncı
[dc.publisher]
Elsevier GmbH
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
The viability of a variety of wheat seeds was investigated by use of the EPR technique. Three different types of spin probes (4-Hydroxy-TEMPO (TANOL), 3-Carbamoyl-2,2,5,5-tetramethyl-3-pyrrolin-1-yloxy, 3-Carbamoyl-PROXYL) were utilized in examining the viability of embryo cells. The penetration of the spin probe across the membrane was observed via the change in signal intensity against time for dry embryos of the variety of wheat seeds soaked in aqueous solutions of spin probe and line broadening agent. From the analysis of the signal intensity-time curves (i.e. rehydration curves) it was observed that the two kinds having different genetic roots (Bezostaya, Kunduru) exhibit similar saturation behaviour whereas Gerek saturates much more easily. The rehydration curves for naturally aged seeds indicates that aging causes rapid saturation and an important decrease in the signal intensity. In order to get better insight on the effect of the signals from polar and nonpolar regions on the experimental spectra, these spectra were simulated by the use of the theoretical models developed.
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Konu Başlıkları
[dc.subject]
EPR
Konu Başlıkları
[dc.subject]
Nitroxide spin probes
Konu Başlıkları
[dc.subject]
Simulation of EPR spectra
Konu Başlıkları
[dc.subject]
Wheat seed
Haklar
[dc.rights]
info:eu-repo/semantics/closedAccess
ISSN
[dc.identifier.issn]
0176-1617
Sponsor YAYINCI
[dc.description.sponsorship]
We would like to thank TOBilTAK (The Scientific and Technical Research Council of Turkey) for the financial suppon of the project. We also wish to thank Prof. Fevzi Kaksal from 19 MaytS Oniversitesi-Samsun for the use of their laboratories for some control measurements.
İlk Sayfa Sayısı
[dc.identifier.startpage]
196
Son Sayfa Sayısı
[dc.identifier.endpage]
200
Dergi Adı
[dc.relation.journal]
Journal of Plant Physiology
Dergi Sayısı
[dc.identifier.issue]
2
Dergi Cilt Bilgisi
[dc.identifier.volume]
151
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.1016/S0176-1617(97)80152-6
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/4202
Görüntülenme Sayısı ( Şehir )
Görüntülenme Sayısı ( Ülke )
Görüntülenme Sayısı ( Zaman Dağılımı )
Görüntülenme
9
09.12.2022 tarihinden bu yana
İndirme
1
09.12.2022 tarihinden bu yana
Son Erişim Tarihi
10 Şubat 2024 01:13
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Tıklayınız
signal viability observed saturation curves spectra variety different intensity rehydration similar exhibit saturates easily naturally whereas indicates causes behaviour Kunduru) developed models theoretical simulated experimental regions nonpolar signals effect insight better decrease important (Bezostaya analysis
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