Antigenotoxic properties of Paliurus spina-christi Mill fruits and their active compounds

Background: Paliurus spina-christi Mill. (PS) fruits are widely used for different medical purposes in Turkey. Like in many medicinal herbs the studies concerning their activity, the activities of PS are also not well clarified. The aim of this study is to evaluate the antigenotoxicity of the compounds isolated and identified from the extracts of PS fruits. Methods: The active compounds were separated, isolated, and determined by chromatographic methods and their structural elucidation was performed by Nuclear Magnetic Resonance (NMR) methods. The compounds were obtained from either ethyl acetate (EA) or n-butanol extracts. The cytotoxicities of the compounds using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and the antigenotoxic activities of the compounds using the alkaline single cell gel electrophoresis techniques (comet assay) were evaluated in Chinese hamster lung fibroblast (V79) cell lines. Results: The isolated major compounds were identified as (+/-) catechins and gallocatechin from EA fraction and rutin from n-butanol fraction of PS fruits. Their chemical structures were identified by 1H-NMR, 13C-NMR, HMBC, and HMQC techniques. Half-maximal inhibitory concentration of catechins, gallocatechin, and rutin were found to be 734 µg/mL, 220 µg/mL, and 1004 µg/mL, respectively. The methanolic extract of PS (1-100 µg/mL) alone did not induce DNA single-strand breaks while catechins (1-100 µg/mL), gallocatechin (1-50 µg/mL), and rutin (1-50 µg/mL) significantly reduced H2O2-induced DNA damage. Conclusion: It has been suggested that PS fruits and their compounds catechins, gallocatechin and rutin may have beneficial effects in oxidative DNA damage. It seems that PS fruits may be used in protection of the disorders related to DNA damage. © 2017 The Author(s).

Dergi Adı BMC Complementary and Alternative Medicine
Dergi Cilt Bilgisi 17
Dergi Sayısı 1
Sayfalar -
Yayın Yılı 2017
Eser Adı
[dc.title]
Antigenotoxic properties of Paliurus spina-christi Mill fruits and their active compounds
Yazar
[dc.contributor.author]
Zor M.
Yazar
[dc.contributor.author]
Aydin S.
Yazar
[dc.contributor.author]
Güner N.D.
Yazar
[dc.contributor.author]
Başaran N.
Yazar
[dc.contributor.author]
Başaran A.A.
Yayın Yılı
[dc.date.issued]
2017
Yayıncı
[dc.publisher]
BioMed Central Ltd.
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
Background: Paliurus spina-christi Mill. (PS) fruits are widely used for different medical purposes in Turkey. Like in many medicinal herbs the studies concerning their activity, the activities of PS are also not well clarified. The aim of this study is to evaluate the antigenotoxicity of the compounds isolated and identified from the extracts of PS fruits. Methods: The active compounds were separated, isolated, and determined by chromatographic methods and their structural elucidation was performed by Nuclear Magnetic Resonance (NMR) methods. The compounds were obtained from either ethyl acetate (EA) or n-butanol extracts. The cytotoxicities of the compounds using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and the antigenotoxic activities of the compounds using the alkaline single cell gel electrophoresis techniques (comet assay) were evaluated in Chinese hamster lung fibroblast (V79) cell lines. Results: The isolated major compounds were identified as (+/-) catechins and gallocatechin from EA fraction and rutin from n-butanol fraction of PS fruits. Their chemical structures were identified by 1H-NMR, 13C-NMR, HMBC, and HMQC techniques. Half-maximal inhibitory concentration of catechins, gallocatechin, and rutin were found to be 734 µg/mL, 220 µg/mL, and 1004 µg/mL, respectively. The methanolic extract of PS (1-100 µg/mL) alone did not induce DNA single-strand breaks while catechins (1-100 µg/mL), gallocatechin (1-50 µg/mL), and rutin (1-50 µg/mL) significantly reduced H2O2-induced DNA damage. Conclusion: It has been suggested that PS fruits and their compounds catechins, gallocatechin and rutin may have beneficial effects in oxidative DNA damage. It seems that PS fruits may be used in protection of the disorders related to DNA damage. © 2017 The Author(s).
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Konu Başlıkları
[dc.subject]
Active compounds
Konu Başlıkları
[dc.subject]
Chinese hamster lung fibroblast cells
Konu Başlıkları
[dc.subject]
Comet assay
Konu Başlıkları
[dc.subject]
Homo- and hetero-NMR
Konu Başlıkları
[dc.subject]
MTT assay
Konu Başlıkları
[dc.subject]
Paliurus spina-christi Mill
Haklar
[dc.rights]
info:eu-repo/semantics/openAccess
ISSN
[dc.identifier.issn]
1472-6882
Dergi Adı
[dc.relation.journal]
BMC Complementary and Alternative Medicine
Dergi Sayısı
[dc.identifier.issue]
1
Dergi Cilt Bilgisi
[dc.identifier.volume]
17
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.1186/s12906-017-1732-1
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/4306
Görüntülenme Sayısı ( Şehir )
Görüntülenme Sayısı ( Ülke )
Görüntülenme Sayısı ( Zaman Dağılımı )
Görüntülenme
17
09.12.2022 tarihinden bu yana
İndirme
1
09.12.2022 tarihinden bu yana
Son Erişim Tarihi
09 Şubat 2024 12:20
Google Kontrol
Tıklayınız
compounds fruits catechins µg/mL) gallocatechin isolated identified damage µg/mL (1-100 methods extracts techniques n-butanol activities fraction Results concentration inhibitory structures Half-maximal 1H-NMR 13C-NMR chemical Background Author(s) related disorders protection oxidative effects beneficial suggested respectively Conclusion
6698 sayılı Kişisel Verilerin Korunması Kanunu kapsamında yükümlülüklerimiz ve çerez politikamız hakkında bilgi sahibi olmak için alttaki bağlantıyı kullanabilirsiniz.

creativecommons
Bu site altında yer alan tüm kaynaklar Creative Commons Alıntı-GayriTicari-Türetilemez 4.0 Uluslararası Lisansı ile lisanslanmıştır.
Platforms