Application of the electron paramagnetic resonance spin probe technique for detection of irradiated wheat

The fast decay of free radicals makes application of traditional electron paramagnetic resonance (EPR) techniques impractical for detection of irradiated wheats when the storage time is longer than 30 days. In this study, therefore, the effects of irradiation on wheat seeds were investigated by using the EPR spin probe technique. The technique is based on the ability of the spin probes to transfer valuable information related to the changes in the structural characteristics of embryo cell membranes caused by irradiation. As a result of irradiation, the environment of the spin probe is modified and this modification can be followed from the recorded spectra. The doses studied were 1.0, 2.5, 10.0, and 20.0 kGy. An aqueous solution of 16- doxyl stearic acid (16-DSA) was used. The embryos were detached using a steel needle and kept in 16-DSA solution for 2.5 hr and washed with distilled water. The spectra of the samples were recorded. The detection of irradiated wheats by this technique was possible at doses of ?2.5 kGy. An important advantage of this approach is that it is applicable even after eight months of storage.

Yazar Sünnetçioglu M.M.
Dadayli D.
Çelik S.
Köksel H.
Yayın Türü Article
Tek Biçim Adres https://hdl.handle.net/20.500.12628/4332
Tek Biçim Adres 10.1094/CCHEM.1998.75.6.875
Koleksiyonlar Araştırma Çıktıları | WoS | Scopus | TR-Dizin | PubMed | SOBİAD
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Dergi Adı Cereal Chemistry
Dergi Cilt Bilgisi 75
Dergi Sayısı 6
Sayfalar 875 - 878
Yayın Yılı 1998
Eser Adı
[dc.title]
Application of the electron paramagnetic resonance spin probe technique for detection of irradiated wheat
Yazar
[dc.contributor.author]
Sünnetçioglu M.M.
Yazar
[dc.contributor.author]
Dadayli D.
Yazar
[dc.contributor.author]
Çelik S.
Yazar
[dc.contributor.author]
Köksel H.
Yayın Yılı
[dc.date.issued]
1998
Yayıncı
[dc.publisher]
American Association of Cereal Chemists
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
The fast decay of free radicals makes application of traditional electron paramagnetic resonance (EPR) techniques impractical for detection of irradiated wheats when the storage time is longer than 30 days. In this study, therefore, the effects of irradiation on wheat seeds were investigated by using the EPR spin probe technique. The technique is based on the ability of the spin probes to transfer valuable information related to the changes in the structural characteristics of embryo cell membranes caused by irradiation. As a result of irradiation, the environment of the spin probe is modified and this modification can be followed from the recorded spectra. The doses studied were 1.0, 2.5, 10.0, and 20.0 kGy. An aqueous solution of 16- doxyl stearic acid (16-DSA) was used. The embryos were detached using a steel needle and kept in 16-DSA solution for 2.5 hr and washed with distilled water. The spectra of the samples were recorded. The detection of irradiated wheats by this technique was possible at doses of ?2.5 kGy. An important advantage of this approach is that it is applicable even after eight months of storage.
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Haklar
[dc.rights]
info:eu-repo/semantics/closedAccess
ISSN
[dc.identifier.issn]
0009-0352
İlk Sayfa Sayısı
[dc.identifier.startpage]
875
Son Sayfa Sayısı
[dc.identifier.endpage]
878
Dergi Adı
[dc.relation.journal]
Cereal Chemistry
Dergi Sayısı
[dc.identifier.issue]
6
Dergi Cilt Bilgisi
[dc.identifier.volume]
75
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.1094/CCHEM.1998.75.6.875
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/4332
Görüntülenme Sayısı ( Şehir )
Görüntülenme Sayısı ( Ülke )
Görüntülenme Sayısı ( Zaman Dağılımı )
Görüntülenme
5
09.12.2022 tarihinden bu yana
İndirme
1
09.12.2022 tarihinden bu yana
Son Erişim Tarihi
06 Eylül 2023 16:45
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Tıklayınız
irradiation technique spectra solution recorded detection irradiated wheats storage aqueous important advantage approach applicable studied months washed distilled 16-DSA needle detached stearic samples embryos possible (16-DSA) membranes followed impractical therefore longer techniques resonance paramagnetic electron
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