Quercetin, a flavonoid antioxidant, prevents and protects streptozotocin-induced oxidative stress and ß-cell damage in rat pancreas

The aim of the present study was the evaluation of possible protective effects of quercetin (QE) against ß-cell damage in experimental streptozotocin (STZ)-induced diabetes in rats. STZ was injected intraperitoneally at a single dose of 50 mg kg -1 for diabetes induction. QE (15 mg kg -1 day, intraperitoneal (i.p.) injection) was injected for 3 days prior to STZ administration; these injections were continued to the end of the study (for 4 weeks). It has been believed that oxidative stress plays a role in the pathogenesis of diabetes mellitus (DM). In order to determine the changes of cellular antioxidant defense system, antioxidant enzymes such as glutathione peroxidase (GSHPx), superoxide dismutase (SOD) and catalase (CAT) activities were measured in pancreatic homogenates. Moreover we also measured serum nitric oxide (NO) and erythrocyte and pancreatic tissue malondialdehyde (MDA) levels, a marker of lipid peroxidation, if there is an imbalance between oxidant and antioxidant status. Pancreatic ß-cells were examined by immunohistochemical methods. STZ induced a significant increase lipid peroxidation, serum NO concentrations and decreased the antioxidant enzyme activity. Erythrocyte MDA, serum NO and pancreatic tissue MDA significantly increased (P < 0.05) and also the antioxidant levels significantly decreased (P < 0.05) in diabetic group. QE treatment significantly decreased the elevated MDA and NO (P < 0.05), and also increased the antioxidant enzyme activities (P < 0.05). QE treatment has shown protective effect possibly through decreasing lipid peroxidation, NO production and increasing antioxidant enzyme activity. Islet cells degeneration and weak insulin immunohistochemical staining was observed in STZ induced diabetic rats. Increased staining of insulin and preservation of islet cells were apparent in the QE-treated diabetic rats. These findings suggest that QE treatment has protective effect in diabetes by decreasing oxidative stress and preservation of pancreatic ß-cell integrity. © 2004 Elsevier Ltd. All rights reserved.

Yazar Coskun O.
Kanter M.
Korkmaz A.
Oter S.
Yayın Türü Article
Tek Biçim Adres https://hdl.handle.net/20.500.12628/7287
Tek Biçim Adres 10.1016/j.phrs.2004.06.002
Konu Başlıkları Diabetes
Oxidative stress
Quercetin
Streptozotocin
Koleksiyonlar Araştırma Çıktıları | WoS | Scopus | TR-Dizin | PubMed | SOBİAD
Scopus İndeksli Yayınlar Koleksiyonu
Dergi Adı Pharmacological Research
Dergi Cilt Bilgisi 51
Dergi Sayısı 2
Sayfalar 117 - 123
Yayın Yılı 2005
Eser Adı
[dc.title]
Quercetin, a flavonoid antioxidant, prevents and protects streptozotocin-induced oxidative stress and ß-cell damage in rat pancreas
Yazar
[dc.contributor.author]
Coskun O.
Yazar
[dc.contributor.author]
Kanter M.
Yazar
[dc.contributor.author]
Korkmaz A.
Yazar
[dc.contributor.author]
Oter S.
Yayın Yılı
[dc.date.issued]
2005
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
The aim of the present study was the evaluation of possible protective effects of quercetin (QE) against ß-cell damage in experimental streptozotocin (STZ)-induced diabetes in rats. STZ was injected intraperitoneally at a single dose of 50 mg kg -1 for diabetes induction. QE (15 mg kg -1 day, intraperitoneal (i.p.) injection) was injected for 3 days prior to STZ administration; these injections were continued to the end of the study (for 4 weeks). It has been believed that oxidative stress plays a role in the pathogenesis of diabetes mellitus (DM). In order to determine the changes of cellular antioxidant defense system, antioxidant enzymes such as glutathione peroxidase (GSHPx), superoxide dismutase (SOD) and catalase (CAT) activities were measured in pancreatic homogenates. Moreover we also measured serum nitric oxide (NO) and erythrocyte and pancreatic tissue malondialdehyde (MDA) levels, a marker of lipid peroxidation, if there is an imbalance between oxidant and antioxidant status. Pancreatic ß-cells were examined by immunohistochemical methods. STZ induced a significant increase lipid peroxidation, serum NO concentrations and decreased the antioxidant enzyme activity. Erythrocyte MDA, serum NO and pancreatic tissue MDA significantly increased (P < 0.05) and also the antioxidant levels significantly decreased (P < 0.05) in diabetic group. QE treatment significantly decreased the elevated MDA and NO (P < 0.05), and also increased the antioxidant enzyme activities (P < 0.05). QE treatment has shown protective effect possibly through decreasing lipid peroxidation, NO production and increasing antioxidant enzyme activity. Islet cells degeneration and weak insulin immunohistochemical staining was observed in STZ induced diabetic rats. Increased staining of insulin and preservation of islet cells were apparent in the QE-treated diabetic rats. These findings suggest that QE treatment has protective effect in diabetes by decreasing oxidative stress and preservation of pancreatic ß-cell integrity. © 2004 Elsevier Ltd. All rights reserved.
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Konu Başlıkları
[dc.subject]
Diabetes
Konu Başlıkları
[dc.subject]
Oxidative stress
Konu Başlıkları
[dc.subject]
Quercetin
Konu Başlıkları
[dc.subject]
Streptozotocin
Haklar
[dc.rights]
info:eu-repo/semantics/closedAccess
ISSN
[dc.identifier.issn]
1043-6618
İlk Sayfa Sayısı
[dc.identifier.startpage]
117
Son Sayfa Sayısı
[dc.identifier.endpage]
123
Dergi Adı
[dc.relation.journal]
Pharmacological Research
Dergi Sayısı
[dc.identifier.issue]
2
Dergi Cilt Bilgisi
[dc.identifier.volume]
51
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.1016/j.phrs.2004.06.002
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/7287
Görüntülenme Sayısı ( Şehir )
Görüntülenme Sayısı ( Ülke )
Görüntülenme Sayısı ( Zaman Dağılımı )
Görüntülenme
19
09.12.2022 tarihinden bu yana
İndirme
1
09.12.2022 tarihinden bu yana
Son Erişim Tarihi
09 Şubat 2024 23:45
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Tıklayınız
antioxidant diabetes pancreatic protective enzyme significantly treatment peroxidation decreased diabetic measured decreasing tissue immunohistochemical effect oxidative induced stress increased insulin preservation levels activity injected ß-cell activities staining marker significant methods examined ß-cells Pancreatic status oxidant
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