GnRH agonist stimulates oocyte number and maturation in mice superovulated with eCG and hCG

Aim: This study was designed to investigate the effects of gonadotropin-releasing hormone agonist (GnRHa) on the ovulation rate, the oocyte number and maturation in mice superovulated with equine chorionic gonadotropin (eCG) and human chorionic gonadotropin (hCG). Methods: Thirty female mice, aging 3 months and weighing between 25-30 g, were assigned to the three experimental groups of control, superovulated and GnRHa treated, each containing 10 animals. Control mice were received only the intraperitoneal (i.p.) injection of 0.1 ml 0.9% NaCl. Superovulated mice were given an i.p. injection of 5 IU of eCG followed 48 hr later by 5 IU of hCG injection (i.p.). The GnRHa treated mice were received an intramuscular (i.m.) injection of a 20 mg/kg GnRHa (buserelin) 24 hr before the superovulation with eCG -hCG. Thirteen hr after hCG administration, mice were sacrificed by cervical dislocation. The ovaries and the fallopian tubes were also harvested for counting the corpora lutea and cumulus-enclosed oocytes. Results: The oocyte number and the maturation, ovulation rate, and the corpus luteum number were higher in GnRHa treated mice than controls and superovulated mice. Conclusion: It is concluded that an i.m. injection of the GnRHa 24 hr before the superovulation with eCG-hCG increases the oocyte number and maturation, and ovulation rate, thus, could be used for in vivo and in vitro fertilizations.

Dergi Adı European Journal of General Medicine
Dergi Cilt Bilgisi 2
Dergi Sayısı 1
Sayfalar 1 - 4
Yayın Yılı 2005
Eser Adı
[dc.title]
GnRH agonist stimulates oocyte number and maturation in mice superovulated with eCG and hCG
Yazar
[dc.contributor.author]
Coşkun Ö.
Yazar
[dc.contributor.author]
Kanter M.
Yayın Yılı
[dc.date.issued]
2005
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
Aim: This study was designed to investigate the effects of gonadotropin-releasing hormone agonist (GnRHa) on the ovulation rate, the oocyte number and maturation in mice superovulated with equine chorionic gonadotropin (eCG) and human chorionic gonadotropin (hCG). Methods: Thirty female mice, aging 3 months and weighing between 25-30 g, were assigned to the three experimental groups of control, superovulated and GnRHa treated, each containing 10 animals. Control mice were received only the intraperitoneal (i.p.) injection of 0.1 ml 0.9% NaCl. Superovulated mice were given an i.p. injection of 5 IU of eCG followed 48 hr later by 5 IU of hCG injection (i.p.). The GnRHa treated mice were received an intramuscular (i.m.) injection of a 20 mg/kg GnRHa (buserelin) 24 hr before the superovulation with eCG -hCG. Thirteen hr after hCG administration, mice were sacrificed by cervical dislocation. The ovaries and the fallopian tubes were also harvested for counting the corpora lutea and cumulus-enclosed oocytes. Results: The oocyte number and the maturation, ovulation rate, and the corpus luteum number were higher in GnRHa treated mice than controls and superovulated mice. Conclusion: It is concluded that an i.m. injection of the GnRHa 24 hr before the superovulation with eCG-hCG increases the oocyte number and maturation, and ovulation rate, thus, could be used for in vivo and in vitro fertilizations.
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Konu Başlıkları
[dc.subject]
GnRH agonist
Konu Başlıkları
[dc.subject]
Mice
Konu Başlıkları
[dc.subject]
Oocyte maturation
Konu Başlıkları
[dc.subject]
Superovulation
Haklar
[dc.rights]
info:eu-repo/semantics/closedAccess
ISSN
[dc.identifier.issn]
1304-3889
İlk Sayfa Sayısı
[dc.identifier.startpage]
1
Son Sayfa Sayısı
[dc.identifier.endpage]
4
Dergi Adı
[dc.relation.journal]
European Journal of General Medicine
Dergi Sayısı
[dc.identifier.issue]
1
Dergi Cilt Bilgisi
[dc.identifier.volume]
2
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/6014
Görüntülenme Sayısı ( Şehir )
Görüntülenme Sayısı ( Ülke )
Görüntülenme Sayısı ( Zaman Dağılımı )
Görüntülenme
6
09.12.2022 tarihinden bu yana
İndirme
1
09.12.2022 tarihinden bu yana
Son Erişim Tarihi
09 Şubat 2024 07:21
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Tıklayınız
injection number superovulated maturation oocyte treated ovulation received superovulation before gonadotropin chorionic cervical sacrificed administration dislocation (buserelin) Thirteen ovaries fertilizations increases eCG-hCG concluded Conclusion fallopian controls higher luteum corpus Results oocytes cumulus-enclosed counting harvested corpora
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