Speciation and determination of thiols in biological samples using high performance liquid chromatography-inductively coupled plasma-mass spectrometry and high performance liquid chromatography-Orbitrap MS

An analytical method was developed for the determination of thiols in biological samples. Reverse phase chromatography coupled to ICP quaduropole MS or Orbitrap MS was employed for the separation and detection of thiols. For the determination of total thiols, oxidized thiols were reduced using dithiothreitol (DTT). Reduction efficiencies for species of interest were found to be close to 100%. Reduced thiols were derivatized by p-hydroxymercuribenzoate (PHMB) and then separated on a C8 column. Optimization of the extraction, separation and detection steps of the HPLC-ICP-MS and HPLC-Orbitrap MS methods was carried out. Detection limits for cysteine, homocysteine, selenocysteine, glutathione, selenomethionine and cysteinyl-glycine were found to be 18, 34, 39, 12, 128 and 103fmol, respectively, using HPLC-Orbitrap MS and 730, 1110, 440, 1110 and 580fmol for cysteine, homocysteine, selenocysteine, glutathione, and cysteinyl-glycine using HPLC-ICP-MS. Contrary to expectation, the LODs and RSDs are higher for the HPLC-ICP-MS instrument, therefore HPLC-Orbitrap MS was used for the determination of thiols in yeast samples. Three different brands of baker's yeast and a selenized yeast were analyzed. The GSH and cysteine levels found in these samples ranged from 4.45 to 17.87µmolg-1 and 0.61 to 1.32µmolg-1, respectively. © 2010 Elsevier B.V.

Yazar Bakirdere S.
Bramanti E.
D'ulivo A.
Ataman O.Y.
Mester Z.
Yayın Türü Article
Tek Biçim Adres https://hdl.handle.net/20.500.12628/7592
Tek Biçim Adres 10.1016/j.aca.2010.09.023
Konu Başlıkları Derivatization
Orbitrap
Speciation
Thiol
Yeast
Koleksiyonlar Araştırma Çıktıları | WoS | Scopus | TR-Dizin | PubMed | SOBİAD
Scopus İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu
Dergi Adı Analytica Chimica Acta
Dergi Cilt Bilgisi 680
Dergi Sayısı 01.Feb
Sayfalar 41 - 47
Yayın Yılı 2010
Eser Adı
[dc.title]
Speciation and determination of thiols in biological samples using high performance liquid chromatography-inductively coupled plasma-mass spectrometry and high performance liquid chromatography-Orbitrap MS
Yazar
[dc.contributor.author]
Bakirdere S.
Yazar
[dc.contributor.author]
Bramanti E.
Yazar
[dc.contributor.author]
D'ulivo A.
Yazar
[dc.contributor.author]
Ataman O.Y.
Yazar
[dc.contributor.author]
Mester Z.
Yayın Yılı
[dc.date.issued]
2010
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
An analytical method was developed for the determination of thiols in biological samples. Reverse phase chromatography coupled to ICP quaduropole MS or Orbitrap MS was employed for the separation and detection of thiols. For the determination of total thiols, oxidized thiols were reduced using dithiothreitol (DTT). Reduction efficiencies for species of interest were found to be close to 100%. Reduced thiols were derivatized by p-hydroxymercuribenzoate (PHMB) and then separated on a C8 column. Optimization of the extraction, separation and detection steps of the HPLC-ICP-MS and HPLC-Orbitrap MS methods was carried out. Detection limits for cysteine, homocysteine, selenocysteine, glutathione, selenomethionine and cysteinyl-glycine were found to be 18, 34, 39, 12, 128 and 103fmol, respectively, using HPLC-Orbitrap MS and 730, 1110, 440, 1110 and 580fmol for cysteine, homocysteine, selenocysteine, glutathione, and cysteinyl-glycine using HPLC-ICP-MS. Contrary to expectation, the LODs and RSDs are higher for the HPLC-ICP-MS instrument, therefore HPLC-Orbitrap MS was used for the determination of thiols in yeast samples. Three different brands of baker's yeast and a selenized yeast were analyzed. The GSH and cysteine levels found in these samples ranged from 4.45 to 17.87µmolg-1 and 0.61 to 1.32µmolg-1, respectively. © 2010 Elsevier B.V.
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Konu Başlıkları
[dc.subject]
Derivatization
Konu Başlıkları
[dc.subject]
Orbitrap
Konu Başlıkları
[dc.subject]
Speciation
Konu Başlıkları
[dc.subject]
Thiol
Konu Başlıkları
[dc.subject]
Yeast
Haklar
[dc.rights]
info:eu-repo/semantics/closedAccess
ISSN
[dc.identifier.issn]
0003-2670
Sponsor YAYINCI
[dc.description.sponsorship]
National Rehabilitation Center
Sponsor YAYINCI
[dc.description.sponsorship]
This work was financially supported by NRC , Canada. Sezgin Bakirdere would like to thank Tubitak for financial support throughout this study. The authors thank Peggy McCooeye for her technical support.
İlk Sayfa Sayısı
[dc.identifier.startpage]
41
Son Sayfa Sayısı
[dc.identifier.endpage]
47
Dergi Adı
[dc.relation.journal]
Analytica Chimica Acta
Dergi Sayısı
[dc.identifier.issue]
01.Feb
Dergi Cilt Bilgisi
[dc.identifier.volume]
680
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.1016/j.aca.2010.09.023
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/7592
Görüntülenme Sayısı ( Şehir )
Görüntülenme Sayısı ( Ülke )
Görüntülenme Sayısı ( Zaman Dağılımı )
Görüntülenme
9
09.12.2022 tarihinden bu yana
İndirme
1
09.12.2022 tarihinden bu yana
Son Erişim Tarihi
10 Şubat 2024 00:40
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thiols determination HPLC-ICP-MS cysteine samples HPLC-Orbitrap respectively selenocysteine glutathione homocysteine cysteinyl-glycine detection separation 32µmolg-1 expectation Contrary 580fmol higher 103fmol Elsevier instrument selenized ranged levels analyzed therefore 87µmolg-1 brands different Orbitrap efficiencies Reduction dithiothreitol reduced oxidized
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