Covalent immobilization of lipase onto amine functionalized polypropylene membrane and its application in green apple flavor (ethyl valerate) synthesis
In this study, a functionalized hydrophobic polypropylene chloride membrane (PPC) was prepared by the amination of chlorinated polypropylene with hexamethylene diamine (APP). The PPC and APP membranes were characterized using SEM, FTIR and contact angle studies. The aminated polypropylene (APP) membrane was used for covalent immobilization of Candida rugosa lipase via glutaraldehyde coupling. The retained activity of the immobilized lipase was 76%. Kinetic analysis shows that the dependence of lipolytic activity of both free and immobilized lipase on tributyrin substrate concentration can be described by Michaelis-Menten model. The estimated apparent Km values for the free and immobilized lipase were 2.9 and 8.4 mM, respectively. The Vmax values of free and immobilized enzymes were calculated as 926 and 741 U/mg enzyme, respectively. Optimal temperature was 5 °C higher for immobilized enzyme than that of the free enzyme. Thermal and storage stabilities were found to be increased upon immobilization. Finally, the immobilized lipase was used for the production of green apple flavor (i.e., ethyl valerate) in hexane medium. © 2010 Elsevier Ltd. All rights reserved.
| Yazar |
Bayramoğlu, Gülay Hazer, Baki Altıntaş, Begüm Arıca, M. Yakup |
| Yayın Türü | Article |
| Tek Biçim Adres | https://hdl.handle.net/20.500.12628/4937 |
| Konu Başlıkları |
Enzyme kinetics
Enzyme stability Ethyl valerate Immobilized enzyme Lipase Polypropylene membrane |
| Koleksiyonlar |
Fakülteler Fen Edebiyat Fakültesi Kimya Bölümü Makale Koleksiyonu (Kimya Bölümü) Araştırma Çıktıları | WoS | Scopus | TR-Dizin | PubMed | SOBİAD Scopus İndeksli Yayınlar Koleksiyonu Araştırma Çıktıları | WoS | Scopus | TR-Dizin | PubMed | SOBİAD WoS İndeksli Yayınlar Koleksiyonu |
| Dergi Adı | Process Biochemistry |
| Dergi Cilt Bilgisi | 46 |
| Dergi Sayısı | 1 |
| Sayfalar | 372 - 378 |
| Yayın Yılı | 2011 |
| Eser Adı [dc.title] | Covalent immobilization of lipase onto amine functionalized polypropylene membrane and its application in green apple flavor (ethyl valerate) synthesis |
| Yazar [dc.contributor.author] | Bayramoğlu, Gülay |
| Yazar [dc.contributor.author] | Hazer, Baki |
| Yazar [dc.contributor.author] | Altıntaş, Begüm |
| Yazar [dc.contributor.author] | Arıca, M. Yakup |
| Yayın Yılı [dc.date.issued] | 2011 |
| Yayın Türü [dc.type] | article |
| Özet [dc.description.abstract] | In this study, a functionalized hydrophobic polypropylene chloride membrane (PPC) was prepared by the amination of chlorinated polypropylene with hexamethylene diamine (APP). The PPC and APP membranes were characterized using SEM, FTIR and contact angle studies. The aminated polypropylene (APP) membrane was used for covalent immobilization of Candida rugosa lipase via glutaraldehyde coupling. The retained activity of the immobilized lipase was 76%. Kinetic analysis shows that the dependence of lipolytic activity of both free and immobilized lipase on tributyrin substrate concentration can be described by Michaelis-Menten model. The estimated apparent Km values for the free and immobilized lipase were 2.9 and 8.4 mM, respectively. The Vmax values of free and immobilized enzymes were calculated as 926 and 741 U/mg enzyme, respectively. Optimal temperature was 5 °C higher for immobilized enzyme than that of the free enzyme. Thermal and storage stabilities were found to be increased upon immobilization. Finally, the immobilized lipase was used for the production of green apple flavor (i.e., ethyl valerate) in hexane medium. © 2010 Elsevier Ltd. All rights reserved. |
| Kayıt Giriş Tarihi [dc.date.accessioned] | 2019-12-23 |
| Açık Erişim Tarihi [dc.date.available] | 2019-12-23 |
| Yayın Dili [dc.language.iso] | eng |
| Konu Başlıkları [dc.subject] | Enzyme kinetics |
| Konu Başlıkları [dc.subject] | Enzyme stability |
| Konu Başlıkları [dc.subject] | Ethyl valerate |
| Konu Başlıkları [dc.subject] | Immobilized enzyme |
| Konu Başlıkları [dc.subject] | Lipase |
| Konu Başlıkları [dc.subject] | Polypropylene membrane |
| Künye [dc.identifier.citation] | Bayramoğlu, G., Hazer, B., Altıntaş, B. ve Arıca, M. Y. (2011). Covalent immobilization of lipase onto amine functionalized polypropylene membrane and its application in green apple flavor (Ethyl valerate) synthesis. Process Biochemistry, 46(1), 372-378. doi:10.1016/j.procbio.2010.09.014 |
| Haklar [dc.rights] | info:eu-repo/semantics/closedAccess |
| ISSN [dc.identifier.issn] | 1359-5113 |
| İlk Sayfa Sayısı [dc.identifier.startpage] | 372 |
| Son Sayfa Sayısı [dc.identifier.endpage] | 378 |
| Dergi Adı [dc.relation.journal] | Process Biochemistry |
| Dergi Sayısı [dc.identifier.issue] | 1 |
| Dergi Cilt Bilgisi [dc.identifier.volume] | 46 |
| Tek Biçim Adres [dc.identifier.uri] | https://dx.doi.org/10.1016/j.procbio.2010.09.014 |
| Tek Biçim Adres [dc.identifier.uri] | https://hdl.handle.net/20.500.12628/4937 |
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