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Antisense oligonucleotide (ASO)-conjugated--tocopherol succinate (TCS)-loaded-poly(lactic acid)-g-poly(ethylene glycol) nanoparticles (ASO-TCS-PLA-PEG NPs), with the ratio of polymer/TCS of 10:2.5, 10:5, 10:7 (w/w) were prepared for targeting cancer therapy. The amphiphilic PLA, amino terminated PEG graft copolymers were synthesized by ring opening polymerization reaction. Nanoparticles were produced by using double emulsion (w/o/w) solvent evaporation method. ASO-TCS-PLA-PEG NPs demonstrated satisfactory encapsulation and loading efficiency and size distribution. The short-term stability studies were carried out at 4 and 25 degrees C for 30days to assess their mean particle size, polydispersity index and zeta potential. The cellular uptake and extended cytoplasmic retention of the NPs in A549 human lung carcinoma and L929 mouse fibroblast cells were examined by fluorescence and confocal microscopy. In human lung cancer cells, ASO-TCS-PLA-PEG NPs exhibited better cellular internalization, cytotoxicity and apoptotic and necrotic effects compared to healthy cell line, L929. These findings showed that ASO-modified nanoparticles could serve as a promising nanocarrier for targeted tumor cells.
| Yazar |
Kılıçay, Ebru Karahaliloğlu, Zeynep Alpaslan, Pınar Hazer, Baki Denkbaş, Emir Baki |
| Yayın Türü | Article |
| Tek Biçim Adres | https://hdl.handle.net/20.500.12628/2763 |
| Konu Başlıkları |
PLA-PEG
antisense oligonucleotide -tocopherol succinate drug delivery human lung cancer cells |
| Koleksiyonlar |
Fakülteler Fen Edebiyat Fakültesi Kimya Bölümü Makale Koleksiyonu (Kimya Bölümü) Meslek Yüksekokulları Zonguldak MYO Makale Koleksiyonu (Zonguldak MYO) Araştırma Çıktıları | WoS | Scopus | TR-Dizin | PubMed | SOBİAD PubMed İndeksli Yayınlar Koleksiyonu Araştırma Çıktıları | WoS | Scopus | TR-Dizin | PubMed | SOBİAD Scopus İndeksli Yayınlar Koleksiyonu Araştırma Çıktıları | WoS | Scopus | TR-Dizin | PubMed | SOBİAD WoS İndeksli Yayınlar Koleksiyonu |
| Dergi Adı | JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION |
| Dergi Cilt Bilgisi | 28 |
| Dergi Sayısı | 15 |
| Sayfalar | 1762 - 1785 |
| Yayın Yılı | 2017 |
| Eser Adı [dc.title] | In vitro evaluation of antisense oligonucleotide functionalized core-shell nanoparticles loaded with -tocopherol succinate |
| Yazar [dc.contributor.author] | Kılıçay, Ebru |
| Yazar [dc.contributor.author] | Karahaliloğlu, Zeynep |
| Yazar [dc.contributor.author] | Alpaslan, Pınar |
| Yazar [dc.contributor.author] | Hazer, Baki |
| Yazar [dc.contributor.author] | Denkbaş, Emir Baki |
| Yayın Yılı [dc.date.issued] | 2017 |
| Yayıncı [dc.publisher] | TAYLOR & FRANCIS LTD |
| Yayın Türü [dc.type] | article |
| Özet [dc.description.abstract] | Antisense oligonucleotide (ASO)-conjugated--tocopherol succinate (TCS)-loaded-poly(lactic acid)-g-poly(ethylene glycol) nanoparticles (ASO-TCS-PLA-PEG NPs), with the ratio of polymer/TCS of 10:2.5, 10:5, 10:7 (w/w) were prepared for targeting cancer therapy. The amphiphilic PLA, amino terminated PEG graft copolymers were synthesized by ring opening polymerization reaction. Nanoparticles were produced by using double emulsion (w/o/w) solvent evaporation method. ASO-TCS-PLA-PEG NPs demonstrated satisfactory encapsulation and loading efficiency and size distribution. The short-term stability studies were carried out at 4 and 25 degrees C for 30days to assess their mean particle size, polydispersity index and zeta potential. The cellular uptake and extended cytoplasmic retention of the NPs in A549 human lung carcinoma and L929 mouse fibroblast cells were examined by fluorescence and confocal microscopy. In human lung cancer cells, ASO-TCS-PLA-PEG NPs exhibited better cellular internalization, cytotoxicity and apoptotic and necrotic effects compared to healthy cell line, L929. These findings showed that ASO-modified nanoparticles could serve as a promising nanocarrier for targeted tumor cells. |
| Açıklama [dc.description] | WOS: 000407503900010 |
| Açıklama [dc.description] | PubMed: 28696185 |
| Kayıt Giriş Tarihi [dc.date.accessioned] | 2019-12-23 |
| Açık Erişim Tarihi [dc.date.available] | 2019-12-23 |
| Yayın Dili [dc.language.iso] | eng |
| Konu Başlıkları [dc.subject] | PLA-PEG |
| Konu Başlıkları [dc.subject] | antisense oligonucleotide |
| Konu Başlıkları [dc.subject] | -tocopherol succinate |
| Konu Başlıkları [dc.subject] | drug delivery |
| Konu Başlıkları [dc.subject] | human lung cancer cells |
| Künye [dc.identifier.citation] | Kilicay, E., Karahaliloglu, Z., Alpaslan, P., Hazer, B. ve Denkbas, E. B. (2017). In vitro evaluation of antisense oligonucleotide functionalized core-shell nanoparticles loaded with -tocopherol succinate. Journal of Biomaterials Science-polymer Edition, 28(15), 1762–1785. doi:10.1080/09205063.2017.1354670 |
| Haklar [dc.rights] | info:eu-repo/semantics/closedAccess |
| ISSN [dc.identifier.issn] | 0920-5063 |
| ISSN [dc.identifier.issn] | 1568-5624 |
| Sponsor YAYINCI [dc.description.sponsorship] | Bulent Ecevit UniversityBulent Ecevit University [BEU-2017-YKD-33496813-01] |
| Sponsor YAYINCI [dc.description.sponsorship] | This work was financially supported by Bulent Ecevit University [BEU-2017-YKD-33496813-01]. |
| İlk Sayfa Sayısı [dc.identifier.startpage] | 1762 |
| Son Sayfa Sayısı [dc.identifier.endpage] | 1785 |
| Dergi Adı [dc.relation.journal] | JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION |
| Dergi Sayısı [dc.identifier.issue] | 15 |
| Dergi Cilt Bilgisi [dc.identifier.volume] | 28 |
| Tek Biçim Adres [dc.identifier.uri] | https://dx.doi.org/10.1080/09205063.2017.1354670 |
| Tek Biçim Adres [dc.identifier.uri] | https://hdl.handle.net/20.500.12628/2763 |