Optimisation of bacillus amyloliquefaciens FE-K1 extracellular peptidase production by response surface methodology
In this study, it was aimed to optimise the extracellular peptidase production of Bacillus amyloliquefaciens FE-K1, previously isolated from ropy wholemeal bread, by using response surface methodology (RSM) based on central composite design (CCD). The temperature (20-45°C), initial pH of the enzyme production medium (pH 5-9) and inoculation level (1-5%, v/v) were used as the factors for RSM, and the fermentation time was determined for each trial separately. Results showed that the optimum peptidase production occurred at 33.4°C, pH 6.62 and 2.3% inoculation. It was determined that the fermentation time was only 7h, the crude enzyme had a peptidase activity of 49.17U/mL and a specific activity of 504.77U/mg under the optimised conditions.
| Yazar |
Erem, Fundagül İnan, Mehmet Certel, Muharrem |
| Yayın Türü | Article |
| Tek Biçim Adres | https://hdl.handle.net/20.500.12628/9419 |
| Konu Başlıkları |
Bacillus
central composite design peptidase response surface methodology |
| Koleksiyonlar |
Fakülteler Mühendislik Fakültesi Gıda Mühendisliği Bölümü Makale Koleksiyonu (Gıda Mühendisliği Bölümü) Araştırma Çıktıları | WoS | Scopus | TR-Dizin | PubMed | SOBİAD TR-Dizin İndeksli Yayınlar Koleksiyonu |
| Dergi Cilt Bilgisi | 19 |
| Dergi Sayısı | 2 |
| Sayfalar | 159 - 173 |
| Yayın Yılı | 2018 |
| Eser Adı [dc.title] | Optimisation of bacillus amyloliquefaciens FE-K1 extracellular peptidase production by response surface methodology |
| Yazar [dc.contributor.author] | Erem, Fundagül |
| Yazar [dc.contributor.author] | İnan, Mehmet |
| Yazar [dc.contributor.author] | Certel, Muharrem |
| Yayın Yılı [dc.date.issued] | 2018 |
| Yayın Türü [dc.type] | article |
| Özet [dc.description.abstract] | In this study, it was aimed to optimise the extracellular peptidase production of Bacillus amyloliquefaciens FE-K1, previously isolated from ropy wholemeal bread, by using response surface methodology (RSM) based on central composite design (CCD). The temperature (20-45°C), initial pH of the enzyme production medium (pH 5-9) and inoculation level (1-5%, v/v) were used as the factors for RSM, and the fermentation time was determined for each trial separately. Results showed that the optimum peptidase production occurred at 33.4°C, pH 6.62 and 2.3% inoculation. It was determined that the fermentation time was only 7h, the crude enzyme had a peptidase activity of 49.17U/mL and a specific activity of 504.77U/mg under the optimised conditions. |
| Kayıt Giriş Tarihi [dc.date.accessioned] | 2021-03-26 |
| Açık Erişim Tarihi [dc.date.available] | 2021-03-26 |
| Yayın Dili [dc.language.iso] | eng |
| Konu Başlıkları [dc.subject] | Bacillus |
| Konu Başlıkları [dc.subject] | central composite design |
| Konu Başlıkları [dc.subject] | peptidase |
| Konu Başlıkları [dc.subject] | response surface methodology |
| Künye [dc.identifier.citation] | Erem, F., İnan, M. ve Certel, M. (2018). Optimisation of bacillus amyloliquefaciens fe-k1 extracellular peptidase production by response surface methodology. Trakya University Journal of Natural Sciences, 19(2), 159-173. |
| Haklar [dc.rights] | info:eu-repo/semantics/openAccess |
| İlk Sayfa Sayısı [dc.identifier.startpage] | 159 |
| Son Sayfa Sayısı [dc.identifier.endpage] | 173 |
| Dergi Sayısı [dc.identifier.issue] | 2 |
| Dergi Cilt Bilgisi [dc.identifier.volume] | 19 |
| Tek Biçim Adres [dc.identifier.uri] | https://hdl.handle.net/20.500.12628/9419 |
Tam Metin
Görüntülenme
Şehir
Görüntülenme
Ülke
İndirme
Şehir
İndirme
Ülke
Görüntülenme & İndirme
Görüntülenme
9
09.12.2022 tarihinden bu yana
İndirme
1
09.12.2022 tarihinden bu yana
Son Erişim Tarihi
02 Mayıs 2023 11:30
Google Kontrol
Tıklayınız
Altmetric
peptidase
production
inoculation
determined
activity
enzyme
fermentation
factors
separately
Results
showed
optimum
occurred
conditions
optimised
77U/mg
specific
17U/mL
wholemeal
isolated
previously
amyloliquefaciens
response
Bacillus
extracellular
optimise
surface
(20-45°C)
medium
initial
temperature
methodology
design
composite
central
