Designing siRNA-conjugated plant oil-based nanoparticles for gene silencing and cancer therapy
In this study, the anticancer activities of two siRNA carriers were compared using a human lung adenocarcinoma epithelial cell line (A549). Firstly, poly(styrene)-graft-poly(linoleic acid) (PS-g-PLina) and poly(styrene)-graft-poly(linoleic acid)-graft-poly(ethylene glycol) (PS-g-PLina-g-PEG) graft copolymers were synthesized by free-radical polymerization. PS-PLina and PS-PLina-PEG nanoparticles (NPs) were prepared by solvent evaporation method and were then characterized. The size was found as 150 ± 10 nm for PS-PLina and 184 ± 6 nm for PS-PLina-PEG NPs. The NPs were functionalized with poly(l-lysine) (PLL) for c-myc siRNA conjugation. siRNA entrapment efficiencies were found in the range of 4–63% for PS-PLina-PLL and 6–42% for PS-PLina-PEG-PLL NPs. The short-term stability test was realised for 1 month. siRNA release profiles were also investigated. In vitro anticancer activity of siRNA-NPs was determined by MTT, flow cytometry, and fluorescence microscopy analyses. Obtained findings showed that both NPs systems were promising as siRNA delivery tool for lung cancer therapy. © 2019, © 2019 Informa UK Limited, trading as Taylor & Francis Group.
| Yazar |
Anılmış, Nur Merve Kara, Göknur Kılıçay, Ebru Hazer, Baki Denkbaş, Emir Baki |
| Yayın Türü | Article |
| Tek Biçim Adres | https://hdl.handle.net/20.500.12628/5018 |
| Konu Başlıkları |
lung cancer
nanoparticles PS-g-PLina PS-g-PLina-PEG siRNA |
| Koleksiyonlar |
Fakülteler Fen Edebiyat Fakültesi Kimya Bölümü Makale Koleksiyonu (Kimya Bölümü) Meslek Yüksekokulları Zonguldak MYO Makale Koleksiyonu (Zonguldak MYO) Araştırma Çıktıları | WoS | Scopus | TR-Dizin | PubMed | SOBİAD Scopus İndeksli Yayınlar Koleksiyonu Araştırma Çıktıları | WoS | Scopus | TR-Dizin | PubMed | SOBİAD WoS İndeksli Yayınlar Koleksiyonu |
| Dergi Adı | Journal of Microencapsulation |
| Dergi Cilt Bilgisi | 36 |
| Dergi Sayısı | 7 |
| Sayfalar | 635 - 648 |
| Yayın Yılı | 2019 |
| Eser Adı [dc.title] | Designing siRNA-conjugated plant oil-based nanoparticles for gene silencing and cancer therapy |
| Yazar [dc.contributor.author] | Anılmış, Nur Merve |
| Yazar [dc.contributor.author] | Kara, Göknur |
| Yazar [dc.contributor.author] | Kılıçay, Ebru |
| Yazar [dc.contributor.author] | Hazer, Baki |
| Yazar [dc.contributor.author] | Denkbaş, Emir Baki |
| Yayın Yılı [dc.date.issued] | 2019 |
| Yayıncı [dc.publisher] | Taylor and Francis Ltd |
| Yayın Türü [dc.type] | article |
| Özet [dc.description.abstract] | In this study, the anticancer activities of two siRNA carriers were compared using a human lung adenocarcinoma epithelial cell line (A549). Firstly, poly(styrene)-graft-poly(linoleic acid) (PS-g-PLina) and poly(styrene)-graft-poly(linoleic acid)-graft-poly(ethylene glycol) (PS-g-PLina-g-PEG) graft copolymers were synthesized by free-radical polymerization. PS-PLina and PS-PLina-PEG nanoparticles (NPs) were prepared by solvent evaporation method and were then characterized. The size was found as 150 ± 10 nm for PS-PLina and 184 ± 6 nm for PS-PLina-PEG NPs. The NPs were functionalized with poly(l-lysine) (PLL) for c-myc siRNA conjugation. siRNA entrapment efficiencies were found in the range of 4–63% for PS-PLina-PLL and 6–42% for PS-PLina-PEG-PLL NPs. The short-term stability test was realised for 1 month. siRNA release profiles were also investigated. In vitro anticancer activity of siRNA-NPs was determined by MTT, flow cytometry, and fluorescence microscopy analyses. Obtained findings showed that both NPs systems were promising as siRNA delivery tool for lung cancer therapy. © 2019, © 2019 Informa UK Limited, trading as Taylor & Francis Group. |
| Kayıt Giriş Tarihi [dc.date.accessioned] | 2019-12-23 |
| Açık Erişim Tarihi [dc.date.available] | 2019-12-23 |
| Yayın Dili [dc.language.iso] | eng |
| Konu Başlıkları [dc.subject] | lung cancer |
| Konu Başlıkları [dc.subject] | nanoparticles |
| Konu Başlıkları [dc.subject] | PS-g-PLina |
| Konu Başlıkları [dc.subject] | PS-g-PLina-PEG |
| Konu Başlıkları [dc.subject] | siRNA |
| Künye [dc.identifier.citation] | Anilmis, N. M., Kara, G., Kilicay, E., Hazer, B. ve Denkbas, E. B. (2019). Designing siRNA-conjugated plant oil-based nanoparticles for gene silencing and cancer therapy. Journal of Microencapsulation, 36(7), 635-648. doi:10.1080/02652048.2019.1665117 |
| Haklar [dc.rights] | info:eu-repo/semantics/closedAccess |
| ISSN [dc.identifier.issn] | 0265-2048 |
| Sponsor YAYINCI [dc.description.sponsorship] | BAGP-001, BEU-2016, 33496813-01 |
| Sponsor YAYINCI [dc.description.sponsorship] | This work was financially supported by Bulent Ecevit University Research Fund (Grant no. BEU-2016?33496813-01) and Kapadokya University #K?N(0).2018-BAGP-001. The authors thank Faruk Bahad?r for helping us to the preparation step of polymer. |
| İlk Sayfa Sayısı [dc.identifier.startpage] | 635 |
| Son Sayfa Sayısı [dc.identifier.endpage] | 648 |
| Dergi Adı [dc.relation.journal] | Journal of Microencapsulation |
| Dergi Sayısı [dc.identifier.issue] | 7 |
| Dergi Cilt Bilgisi [dc.identifier.volume] | 36 |
| Tek Biçim Adres [dc.identifier.uri] | https://dx.doi.org/10.1080/02652048.2019.1665117 |
| Tek Biçim Adres [dc.identifier.uri] | https://hdl.handle.net/20.500.12628/5018 |
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