The effect of sildenafil on an animal model for ischemic colitis

Introduction Sildenafil both enhances vasodilatation by relaxing the smooth muscle in the vessels and inhibits platelet aggregation. We have therefore examined the potential benefits of sildenafil on an animal model for ischemic colitis (IC). Methods Twenty-eight female Wistar albino rats weighing 250-300 g were randomized into three experimental groups as follows: in Group 1, animals were sham operated (n = 8) and received tap water; in Groups 2 and 3, the rats underwent a standardized surgical procedure to induce IC (n = 10 in each group). Group 2 animals served as the controls, receiving only tap water, while Group 3 animals received 10 mg/kg sildenafil per day as a single dose for a 3-day period. All animals were sacrificed 72 h after devascularization. To determine the severity of the ischemia, we scored the macroscopically visible damage, measured the ischemic area and scored the histopathology. Tissue malondialdehyde levels were also evaluated. Results The mean area of ischemic changes were 116.80 +/- 189.93 and 0.55 +/- 1.01 mm(2) in Group 2 and 3 animals, respectively (p = 0.0001), while the macroscopically mean visible damage score decreased to 0.66 +/- 0.70 (p = 0.0001) for Group 3 animals. The Chiu scores were 0.00, 3.80 +/- 0.91 and 2.66 +/- 1.00 in Group 1, 2 and 3 animals, respectively. There was a statistically significant difference between Group 2 and 3 animals (p = 0.017). Conclusions Our findings support the view that sildenafil leads to a improvement in IC due to its well-known effects on the vascular smooth muscle and on the microcirculatory hemodynamics.

Yazar İrkörücü, Oktay
Taşçılar, Öğe
Çakmak, Güldeniz Karadeniz
Karakaya, Kemal
Emre, Ali Uğur
Uçan, Bülent Hamdi
Bahadır, Burak
Açıkgöz, Seferden
Ankaralı, Handan
Uğurbaş, Ebru
Cömert, Mustafa
Yayın Türü Article
Tek Biçim Adres https://hdl.handle.net/20.500.12628/3529
Tek Biçim Adres 10.1007/s10620-007-0033-9
Konu Başlıkları ischemic colitis
ischemic colon
PDE inhibitor
sildenafil
Koleksiyonlar Fakülteler
Tıp Fakültesi
Cerrahi Bilimler Bölümü
Araştırma Çıktıları | WoS | Scopus | TR-Dizin | PubMed | SOBİAD
Makale Koleksiyonu (Cerrahi Bilimler Bölümü)
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu
Dergi Adı DIGESTIVE DISEASES AND SCIENCES
Dergi Cilt Bilgisi 53
Dergi Sayısı 6
Sayfalar 1618 - 1623
Yayın Yılı 2008
Eser Adı
[dc.title]
The effect of sildenafil on an animal model for ischemic colitis
Yazar
[dc.contributor.author]
İrkörücü, Oktay
Yazar
[dc.contributor.author]
Taşçılar, Öğe
Yazar
[dc.contributor.author]
Çakmak, Güldeniz Karadeniz
Yazar
[dc.contributor.author]
Karakaya, Kemal
Yazar
[dc.contributor.author]
Emre, Ali Uğur
Yazar
[dc.contributor.author]
Uçan, Bülent Hamdi
Yazar
[dc.contributor.author]
Bahadır, Burak
Yazar
[dc.contributor.author]
Açıkgöz, Seferden
Yazar
[dc.contributor.author]
Ankaralı, Handan
Yazar
[dc.contributor.author]
Uğurbaş, Ebru
Yazar
[dc.contributor.author]
Cömert, Mustafa
Yayın Yılı
[dc.date.issued]
2008
Yayıncı
[dc.publisher]
SPRINGER
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
Introduction Sildenafil both enhances vasodilatation by relaxing the smooth muscle in the vessels and inhibits platelet aggregation. We have therefore examined the potential benefits of sildenafil on an animal model for ischemic colitis (IC). Methods Twenty-eight female Wistar albino rats weighing 250-300 g were randomized into three experimental groups as follows: in Group 1, animals were sham operated (n = 8) and received tap water; in Groups 2 and 3, the rats underwent a standardized surgical procedure to induce IC (n = 10 in each group). Group 2 animals served as the controls, receiving only tap water, while Group 3 animals received 10 mg/kg sildenafil per day as a single dose for a 3-day period. All animals were sacrificed 72 h after devascularization. To determine the severity of the ischemia, we scored the macroscopically visible damage, measured the ischemic area and scored the histopathology. Tissue malondialdehyde levels were also evaluated. Results The mean area of ischemic changes were 116.80 +/- 189.93 and 0.55 +/- 1.01 mm(2) in Group 2 and 3 animals, respectively (p = 0.0001), while the macroscopically mean visible damage score decreased to 0.66 +/- 0.70 (p = 0.0001) for Group 3 animals. The Chiu scores were 0.00, 3.80 +/- 0.91 and 2.66 +/- 1.00 in Group 1, 2 and 3 animals, respectively. There was a statistically significant difference between Group 2 and 3 animals (p = 0.017). Conclusions Our findings support the view that sildenafil leads to a improvement in IC due to its well-known effects on the vascular smooth muscle and on the microcirculatory hemodynamics.
Açıklama
[dc.description]
WOS: 000255955700024
Açıklama
[dc.description]
PubMed: 17932755
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Konu Başlıkları
[dc.subject]
ischemic colitis
Konu Başlıkları
[dc.subject]
ischemic colon
Konu Başlıkları
[dc.subject]
PDE inhibitor
Konu Başlıkları
[dc.subject]
sildenafil
Künye
[dc.identifier.citation]
Irkorucu, O., Tascilar, O., Cakmak, G. K., Karakaya, K., Emre, A. U., Ucan, B. H., … Comert, M. (2008). The effect of sildenafil on an animal model for ischemic colitis. Digestive Diseases and Sciences, 53(6), 1618-1623. doi:10.1007/s10620-007-0033-9
Haklar
[dc.rights]
info:eu-repo/semantics/closedAccess
ISSN
[dc.identifier.issn]
0163-2116
İlk Sayfa Sayısı
[dc.identifier.startpage]
1618
Son Sayfa Sayısı
[dc.identifier.endpage]
1623
Dergi Adı
[dc.relation.journal]
DIGESTIVE DISEASES AND SCIENCES
Dergi Sayısı
[dc.identifier.issue]
6
Dergi Cilt Bilgisi
[dc.identifier.volume]
53
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.1007/s10620-007-0033-9
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/3529
Görüntülenme Sayısı ( Şehir )
Görüntülenme Sayısı ( Ülke )
Görüntülenme Sayısı ( Zaman Dağılımı )
Görüntülenme
343
09.12.2022 tarihinden bu yana
İndirme
1
09.12.2022 tarihinden bu yana
Son Erişim Tarihi
30 Mayıs 2024 08:58
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Tıklayınız
animals ischemic sildenafil scored macroscopically visible respectively smooth muscle damage received changes Tissue histopathology measured Results malondialdehyde evaluated levels ischemia Introduction Conclusions hemodynamics microcirculatory vascular effects well-known improvement support findings between difference significant statistically scores
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