Beneficial effects of Ebselen on corrosive esophageal burns of rats

Aim: This study was evaluated to investigate the efficacy of Ebselen, which is an organoselenium compound and glutathione peroxidase mimic, on the prevention of stricture development after esophageal caustic injuries in the rat. Methods: Thirty healthy male Wistar albino rats were utilized in this study. The rats were randomly allotted into one of three experimental groups: group A (sham) animals were uninjured. Caustic esophageal burn was created by applying 1 ml of 37.5% NaOH to the distal esophagus. Group B rats were injured but untreated. Group C rats were injured and received Ebselen (10 mg/kg/day) via the oral route. Blood and tissue samples for the biochemical and histopathological analysis were taken all rats at the end (28th day) of the experiment. Oxidative stress is believed to play a role in the pathogenesis of corrosive esophageal burns. To assess changes in the cellular antioxidant defense system, we measured the activities of antioxidant enzymes (such as glutathione peroxidase (GSHPx), superoxide dismutase (SOD), and catalase (CAT)) in esophagus homogenates. We also measured esophageal tissue malondialdehyde (MDA) levels, a marker of lipid peroxidation, to determine whether there is an imbalance between oxidant and antioxidant status. Efficacy of the treatment was assessed by measuring the stenosis index and histopathologic damage score and biochemically by determining tissue hydroxyproline content, lipid peroxidation and antioxidant enzyme levels. Results: The stenosis index in group B was significantly increased compared with group A and C (P < 0.05). The hydroxyproline level was significantly increased in group B compared with group A and C (P < 0.05). In group B, the histopathologic damage score was significantly higher than in group C (P < 0.05). Treatment with Ebselen decreased tissue hydroxyproline levels, histological damage, and the stenosis index. Caustic esophageal burn increased the lipid peroxidation and also decreased the antioxidant enzyme levels in group B. Ebselen treatments for 28 days decreased the elevated lipid peroxidation and also increased the reduced antioxidant enzyme levels. Live weights of the rats was significantly decreased in group B compared with group A and C (P < 0.05). Conclusion: It is concluded that Ebselen has a preventive effect in the development of fibrosis and decrease the lipid peroxidation, and increase the antioxidant defense system activity in an experimental model of corrosive esophagitis in rats. © 2005 Elsevier Ireland Ltd. All rights reserved.

Yazar Ocakci A.
Coskun O.
Tumkaya L.
Kanter M.
Gurel A.
Hosnuter M.
Uzun L.
Yayın Türü Article
Tek Biçim Adres https://hdl.handle.net/20.500.12628/4480
Tek Biçim Adres 10.1016/j.ijporl.2005.05.018
Konu Başlıkları Antioxidant enzymes
Caustic burn
Corrosive esophagitis
Ebselen
Fibrosis
Lipid peroxidation
Rat
Stricture
Koleksiyonlar Araştırma Çıktıları | WoS | Scopus | TR-Dizin | PubMed | SOBİAD
Scopus İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu
Dergi Adı International Journal of Pediatric Otorhinolaryngology
Dergi Cilt Bilgisi 70
Dergi Sayısı 1
Sayfalar 45 - 52
Yayın Yılı 2006
Eser Adı
[dc.title]
Beneficial effects of Ebselen on corrosive esophageal burns of rats
Yazar
[dc.contributor.author]
Ocakci A.
Yazar
[dc.contributor.author]
Coskun O.
Yazar
[dc.contributor.author]
Tumkaya L.
Yazar
[dc.contributor.author]
Kanter M.
Yazar
[dc.contributor.author]
Gurel A.
Yazar
[dc.contributor.author]
Hosnuter M.
Yazar
[dc.contributor.author]
Uzun L.
Yayın Yılı
[dc.date.issued]
2006
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
Aim: This study was evaluated to investigate the efficacy of Ebselen, which is an organoselenium compound and glutathione peroxidase mimic, on the prevention of stricture development after esophageal caustic injuries in the rat. Methods: Thirty healthy male Wistar albino rats were utilized in this study. The rats were randomly allotted into one of three experimental groups: group A (sham) animals were uninjured. Caustic esophageal burn was created by applying 1 ml of 37.5% NaOH to the distal esophagus. Group B rats were injured but untreated. Group C rats were injured and received Ebselen (10 mg/kg/day) via the oral route. Blood and tissue samples for the biochemical and histopathological analysis were taken all rats at the end (28th day) of the experiment. Oxidative stress is believed to play a role in the pathogenesis of corrosive esophageal burns. To assess changes in the cellular antioxidant defense system, we measured the activities of antioxidant enzymes (such as glutathione peroxidase (GSHPx), superoxide dismutase (SOD), and catalase (CAT)) in esophagus homogenates. We also measured esophageal tissue malondialdehyde (MDA) levels, a marker of lipid peroxidation, to determine whether there is an imbalance between oxidant and antioxidant status. Efficacy of the treatment was assessed by measuring the stenosis index and histopathologic damage score and biochemically by determining tissue hydroxyproline content, lipid peroxidation and antioxidant enzyme levels. Results: The stenosis index in group B was significantly increased compared with group A and C (P < 0.05). The hydroxyproline level was significantly increased in group B compared with group A and C (P < 0.05). In group B, the histopathologic damage score was significantly higher than in group C (P < 0.05). Treatment with Ebselen decreased tissue hydroxyproline levels, histological damage, and the stenosis index. Caustic esophageal burn increased the lipid peroxidation and also decreased the antioxidant enzyme levels in group B. Ebselen treatments for 28 days decreased the elevated lipid peroxidation and also increased the reduced antioxidant enzyme levels. Live weights of the rats was significantly decreased in group B compared with group A and C (P < 0.05). Conclusion: It is concluded that Ebselen has a preventive effect in the development of fibrosis and decrease the lipid peroxidation, and increase the antioxidant defense system activity in an experimental model of corrosive esophagitis in rats. © 2005 Elsevier Ireland Ltd. All rights reserved.
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Konu Başlıkları
[dc.subject]
Antioxidant enzymes
Konu Başlıkları
[dc.subject]
Caustic burn
Konu Başlıkları
[dc.subject]
Corrosive esophagitis
Konu Başlıkları
[dc.subject]
Ebselen
Konu Başlıkları
[dc.subject]
Fibrosis
Konu Başlıkları
[dc.subject]
Lipid peroxidation
Konu Başlıkları
[dc.subject]
Rat
Konu Başlıkları
[dc.subject]
Stricture
Haklar
[dc.rights]
info:eu-repo/semantics/closedAccess
ISSN
[dc.identifier.issn]
0165-5876
İlk Sayfa Sayısı
[dc.identifier.startpage]
45
Son Sayfa Sayısı
[dc.identifier.endpage]
52
Dergi Adı
[dc.relation.journal]
International Journal of Pediatric Otorhinolaryngology
Dergi Sayısı
[dc.identifier.issue]
1
Dergi Cilt Bilgisi
[dc.identifier.volume]
70
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.1016/j.ijporl.2005.05.018
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/4480
Görüntülenme Sayısı ( Şehir )
Görüntülenme Sayısı ( Ülke )
Görüntülenme Sayısı ( Zaman Dağılımı )
Görüntülenme
285
09.12.2022 tarihinden bu yana
İndirme
1
09.12.2022 tarihinden bu yana
Son Erişim Tarihi
30 Mayıs 2024 15:39
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Tıklayınız
antioxidant Ebselen esophageal levels peroxidation decreased tissue increased significantly damage compared enzyme stenosis hydroxyproline glutathione esophagus peroxidase defense Caustic histopathologic development corrosive system experimental measured injured homogenates biochemically (CAT)) imbalance malondialdehyde marker determine measuring assessed
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