Kandemir O. | Buyukates M. | Kandemir N.O. | Aktunc E. | Gul A.E. | Gul S. | Turan S.A.

Article | 2010 | Journal of Cardiothoracic Surgery5 ( 1 )

Background: Ankaferd Blood Stopper®(ABS) is a folkloric medicinal plant extract used as a hemostatic agent in traditional Turkish medicine. This experimental study investigated the histopathological and immunohistochemical effects of ABS on vascular tissue in a rat model of aortic bleeding.Methods: Four groups of 11 Wistar albino rats were used. The abdominal aortas of the rats were wounded; an ABS-soaked tampon was applied to rats in Groups 1 and 3, and a plain gauze tampon was applied to rats in Groups 2 and 4 until the bleeding stopped. The bleeding time was recorded. Immediately following sacrificing, the arteriotomy sites from . . .Groups 1 and 2 were removed. The abdominal incisions in Groups 3 and 4 were closed following hemostasis. On Day 7 of the study, Group 3 and 4 rats were sacrificed and the abdominal aorta arteriotomy sites were removed for histopathological and immunohistochemical evaluation.Results: The mean bleeding time in 15 animals in Groups 2 and 4 was 4.9 ± 0.6 s, and in 22 animals in Groups 1 and 3 was 3.1 ± 0.6 s. Distal aortic occlusion was not observed on either Day 1 or 7 in any group. Significantly more widespread and dense endothelial nitric oxide synthase (eNOS) staining was observed in Group 1 animals than Group 2. On Days 1 and 7 after application of ABS, histopathological changes, consisting of necrosis, inflammation, and endothelial cell loss, in the rat abdominal aortas did not differ between Groups 1 and 2. The basophilic discoloration in the ABS group on the operation day was a result of a foreign body reaction and hemosiderin-loaded histiocyte accumulation, which occurred on Day 7.Conclusions: In this study, hemostasis was successfully achieved with ABS in rat abdominal aortas. No histopathological change was found in the rat abdominal aortas between the ABS and control groups on Days 1 and 7. Further studies on the long-term effects of foreign body reactions and hemosiderin-loaded histiocyte accumulation are required. © 2010 Kandemir et al; licensee BioMed Central Ltd Daha fazlası Daha az

Kandemir N.O. | Barut F. | Yilmaz K. | Tokgoz H. | Hosnuter M. | Ozdamar S.O.

Article | 2010 | Case Reports in Medicine2010

Renal cell carcinoma is the most common kidney tumor in adults. Cutaneous metastasis is a rare first symptom of the disease. This paper describes the diagnosis of a renal cell carcinoma that was indicated by cutaneous metastasis in the head and neck region, and considers the etiopathogenesis of such cases. A careful skin examination is important to detect cutaneous metastasis associated with renal cell carcinomas. Metastatic skin lesions in the head and neck region must be taken into consideration during a differential diagnosis. Copyright © 2010 Nilufer Onak Kandemir et al.

Gonca E. | Barut F. | Erdem S.

Article | 2017 | Turkish Journal of Thoracic and Cardiovascular Surgery25 ( 2 ) , pp.273 - 281

Background: This study aims to evaluate the effects of zileuton on myocardial ischemia/reperfusion injury and ischemia-induced ventricular arrhythmias and to investigate the role of 5-lipoxygenase pathway in the pathogenesis of myocardial ischemia/reperfusion injury. Methods: In anesthetized rats, myocardial ischemia was induced by the ligation of the left main coronary artery for 30 min before 120-min reperfusion period. Zileuton was given both at the doses of 3 and 10 mg/kg 15 min before the ligation. During the experiment, electrocardiography, blood pressure, and heart rate were recorded. The duration of arrhythmia types were det . . .ermined during the ischemic period. To evaluate the ischemia/reperfusion injury in the myocardial tissue, histopathological examination was performed and the infarct size was determined by 2,3,5-triphenyltetrazolium chloride staining. Results: Zileuton at a dose of 3 mg/kg significantly decreased the infarct size and the tissue injury score obtained using histopathological examinations (infarct size [% of the area at risk]: zileuton 3 mg/kg 36±7% versus control 66±6%, p < 0.05). Zileuton 10 mg/kg was found to be ineffective. Both 3 and 10 mg/kg doses of zileuton did not shorten the duration of arrhythmias during the ischemic period. Conclusion: Our study results showed that 3 mg/kg dose of zileuton protected the heart against myocardial ischemia/ reperfusion injury. However, it was ineffective to reduce the ischemia-induced ventricular arrhythmias. Based on these results, zileuton may be a promising drug for the treatment of myocardial ischemia/reperfusion injury. © 2017. Turkish Society of Cardiovascular Surgery Daha fazlası Daha az

Bahadır, Burak | Kandemir, Nilüfer Onak | Karadayı, Nimet | Özdamar, Şükrü Oğuz

Proceedings | 2008 | VIRCHOWS ARCHIV452 , pp.273 - 281

WOS: 000256192600370

Numanoğlu, Kemal Varın | Ayoğlu, Hilal | Tatlı, Duygu | Er, Ebubekir

Article | 2014 | Therapeutics and Clinical Risk Management10 , pp.753 - 758

Background: Preincisional local anesthetic infiltration at the surgical site is a therapeutic option for postoperative pain relief for pediatric inguinal hernia. Additionally, tramadol has been used as an analgesic for postoperative pain in children. Recently, the local anesthetic effects of tramadol have been reported. The aim of this study was to determine both the systemic analgesic and the local anesthetic effects of tramadol and to determine how it differs from bupivacaine when administered preincisionally. Methods: Fifty-two healthy children, aged 2–7 years, who were scheduled for elective herniorrhaphy were randomly allocated . . . to receive either preincisional infiltration at the surgical site with 2 mg/kg tramadol (Group T, n=26) or 0.25 mL/kg 0.5% bupivacaine (Group B, n=26). At the time of anesthetic administration, perioperative hemodynamic parameters were recorded. The pain assessments were performed 10 minutes after the end of anesthesia and during the first 6-hour period, using pain scores. The time of first dose of analgesia and need for additional analgesia were recorded. Results: Between T and B groups, the anesthesia time, perioperative hemodynamic changes, and pain scores were not statistically different. However, in group B, the postoperative analgesic requirement was higher than in group T. Conclusion: Tramadol shows equal analgesic effect to bupivacaine and decreases additional analgesic requirement, when used for preincisional infiltration anesthesia in children undergoing inguinal herniorrhaphy. © 2014 Numanoğlu et al Daha fazlası Daha az

Özaçmak-Sayan, Hale | Özaçmak, Veysel Haktan | Barut, Figen | Araslı, Mehmet | Uçan, Bülent Hamdi

Article | 2014 | Journal of Surgical Research191 ( 2 ) , pp.350 - 361

Background: Spironolactone (Sp), a mineralocorticoid receptor antagonist, protects against the ischemia reperfusion (IR) injury of retina, kidney, heart, and brain. We aimed to investigate the effects of Sp on intestinal IR injury.Methods Male Wistar rats were randomly divided into: (1) a sham control group; (2) an IR control group, subjected to 30 min ischemia and 3 h reperfusion; (3) a group treated with Sp (20 mg/kg) for 3 d before the IR; and (4) a sham-operated control group treated with Sp (20 mg/kg). After the reperfusion, blood and intestinal tissue samples were collected to evaluate histopathologic state, neutrophil infiltr . . .ation (by measuring myeloperoxidase activity), levels of the cytokines (tumor necrosis factor ?, interleukin 1? [IL-1?], interferon ?, monocyte chemotactic protein-1, granulocyte macrophage-colony stimulating factor, and IL-4), malondialdehyde (MDA) and reduced glutathione contents, and immunohistochemical expressions of nuclear factor ?B, inducible nitric oxide synthase (iNOS), and caspase-3. Results MDA content, myeloperoxidase activity, and plasma levels of tumor necrosis factor ?, IL-1?, and monocyte chemotactic protein-1 were all elevated in IR, indicating the oxidative stress and local and systemic inflammatory response. Sp administration markedly reduced the MDA content and the cytokine levels. The pretreatment alleviated intestinal injury, neutrophil infiltration, and the expressions of caspase-3, iNOS, and NF?B.Conclusions: The results implicate that Sp may have a strong protective effect against the intestinal IR injury. The effect can be mediated via suppression of both systemic inflammatory response and apoptosis through amelioration of oxidative stress and generation of proinflammatory cytokines, iNOS, caspase-3, and nuclear factor ?B. Therefore, mineralocorticoid receptor antagonism might be of potential therapeutic benefit in cases of intestinal IR damage. © 2014 Elsevier Inc. All rights reserved Daha fazlası Daha az

Özdamar Ş.O. | Dogan Gün B. | Barut F. | Bahadir B.

Article | 2007 | Turkiye Klinikleri Journal of Medical Sciences27 ( 1 ) , pp.16 - 20

Objective: Traumatic neuroma is an exuberant, probably non-neoplastic proliferation of a nerve occurring in response to injury or surgery. We studied six cases of traumatic neuroma with light microscopic, histochemical and immunohistochemical methods to assess the cellular compositions of these lesions. Material and Methods: Sections from the formalin-fixed parafin-embedded tissues were stained with hematoxylin-eosin, Gomori's trichrome, Verhoeff elastica-von Gieson, reticuline, and S-100 protein, Epithelial Membrane Antigen, CD34 and CD68. Results: All cases revealed large numbers of small and haphazardly arranged regenerating nerv . . .e fascicles within a densely collagenous and fibroblastic stroma. A focal chronic mononuclear cell inflammatory reaction was observed in three cases. In all cases, Gomori's trichrome revealed collagen. Axonal morphology was detected in nerve fibers histochemically by reticulin. In all cases, fascicles were stained diffusely with S-100 protein, and Epithelial Membrane Antigen showed a positive reaction in a thin band of cells surrounding the fascicles in three of the cases. CD34 positive cells were present in five cases. CD68 expressing cells were present in only in two specimens. Conclusion: In traumatic neuromas, specific staining of fascicles with S-100 protein, perineural cells reactive for Epithelial Membrane Antigen and the presence of CD34 positive cells may contribute to our understanding of their pathogenesis and differentiation of these lesions from mimickers. Copyright © 2007 by Türkiye Klinikleri Daha fazlası Daha az

Onak Kandemir N. | Barut F. | Dogan Gün B. | Solak Tekin N. | Hallaç Keser S. | Oguz Özdamar Ş.

Article | 2013 | Case Reports in Medicine2013 , pp.16 - 20

Aim. Cavernous hemangioma-like Kaposi sarcoma is a rare morphologic type of Kaposi sarcoma. So far there are no cases in the literature defining the histological features of this morphologic spectrum in detail. In this study we presented two classical-type cutaneous Kaposi sarcoma cases with histologic findings resembling cavernous hemangioma in company with clinical and histopathological data. Cases. One hundred and eighty-five classical-type cutaneous Kaposi sarcoma lesions in 79 patients were assessed retrospectively in terms of histopathological features. Findings of two cases showing features of cavernous hemangioma-like Kaposi . . . sarcoma whose clinical data could be accessed were presented in accompany with the literature data. Both cases were detected to have bluish-purple, protruded, irregularly bordered cutaneous lesions. Histopathological examination revealed a lesion formed by cavernous hemangioma-like vascular structures organized in a lobular pattern that became dilated and filled with blood. Typical histological findings of early-stage KS, consisting of mononuclear inflammation, extravasated erythrocytes, and a few immature vascular structures in superficial dermis, were observed. All cases were serologically HIV-1 negative. A positive reaction with HHV-8, CD31, CD34, and D2-40 monoclonal antibodies was identified at both cavernous hemangioma-like areas and in immature vascular structures. Results. Cavernous hemangioma-like Kaposi sarcoma is a rare Kaposi sarcoma variant presenting with diagnostic challenges, that may be confused with hemangioma. As characteristic morphological features may not be observed in every case, it is important for diagnostic purposes to show immunohistochemical HHV-8 positivity in this variant. © 2013 Nilüfer Onak Kandemir et al Daha fazlası Daha az

Bektaş S. | Bahadir B. | Barut F. | Bayar Ü. | Özdamar Ş.O.

Article | 2010 | Turkiye Klinikleri Jinekoloji Obstetrik20 ( 6 ) , pp.403 - 406

Ovarian lipoleiomyoma composed of an admixture of smooth muscle and adipose tissue is a rare mesenchymal neoplasm. A 54-year-old female patient presented with pelvic pain. Ultrasonography revealed a giant mass with cystic-solid appearance filling of the Douglas pouch. With suspicion of ovarian carcinoma, operation was performed. Gross examination of the left ovary showed a mass measuring 20 x 19 x 11 cm. Histopathologically, the tumor consisted primarily of interlacing spindle cells with scattered of mature adipose tissue. Immunohistochemically, spindle cells exhibited vimentin, smooth muscle actin and desmin positivity. Adipose cel . . .ls revealed positive reaction for S100 protein. A diagnosis of lipoleiomyoma was made. Ovarian lipoleiomyoma may mimic malignancy, both clinically and radiographically, due the the fact that it may reach a giant size and because of its heterogeneous internal structure. Copyright © 2010 by Türkiye Klinikleri Daha fazlası Daha az

Cihan, Alper | Armutçu, Ferah | Uçan, Bülent H. | Acun, Zeki | Numanoğlu, Kemal Varın | Gürel, Ahmet | Ulukent, Suat Can

Article | 2003 | Hepato-gastroenterology50 Suppl 2 , pp.403 - 406

BACKGROUND/AIMS: This study was designed to determine whether there exists a difference between in vivo and in vitro measurements of bursting pressure (BP) of experimental intestinal anastomosis studied in Wistar-albino rats. METHODOLOGY: In the first group (n=8), the BP was measured using in vivo method without detaching the adhesions around the anastomosis. BP was determined with digital manometer, and then anastomotic region was removed to measure tissue hydroxyproline (HP) levels. In the second group (n=8), the BP was measured with in vitro method after the segment of intestine including the anastomosis was dissected and isolate . . .d. The isolated specimen was then submerged in a normal saline bath. BP was determined with a digital manometer and anastomotic region was removed to measure tissue HP levels. RESULTS: While HP value in the first group was 105.60 +/- 9.43 microg/mg dry tissue, it was found to be 121.11 +/- 16.26 microg/mg dry tissue in the second group and this difference was not statistically significant (p=0.195). The BP was determined as 240.71 +/- 11.65 mmHg in the first group, 144.71 +/- 16.41 mmHg in the second group and the difference was statistically significant (p=0.002). The anastomotic resistances to intraluminal pressure were found to be statistically different whereas tissue HP levels were normal between the groups. CONCLUSIONS: These results make us consider that mechanical changes occur about the isolated anastomotic line and dissection of adhesions weakens the anastomosis Daha fazlası Daha az

Bayar, Ülkü Özmen | Numanoğlu, Kemal Varın | Bektaş, Sibel | Sade, Hakan | Tatlı, Duygu

Article | 2010 | Case Reports in Medicine2010 , pp.403 - 406

Congenital malformations of the lung (CML) are rare with similar embryological and clinical spectra and could result in mortality if left untreated. Bronchogenic cysts are formed during the budding of the tracheal diverticula and ventral foregut in the embryological period. In this paper we want to present a case of bronchogenic cyst with continuous intrauterine cyst aspiration follow-up. After the baby birth was operated and the postoperative period was uneventful. The pathological examination revealed a bronchogenic cyst. Copyright © 2010 Ülkü Özmen Bayar et al.

Gün, Banu Doğan | Numanoğlu, Gamze | Özdamar, Şükrü Oğuz

Article | 2006 | Acta Obstetricia et Gynecologica Scandinavica85 ( 4 ) , pp.402 - 406

Background. To determine whether adequate trophoblastic migration and maternal placental perfusion occurs in cases of early pregnancy loss, we compared vessels in elective abortion decidua with those in spontaneous abortion decidua. Methods. Elective abortion decidua at 5-11 weeks ( n =40) were compared with spontaneous abortion decidua at 5-12 weeks ( n =25). Also normal late secretory endometrial biopsy specimens ( n =10) were examined. The cross-sections of veins and arteries were counted in 25 elective and 15 spontaneous abortion materials. The number of the veins that contain trophoblastic fragments and the number of the spiral . . . arteries converted by trophoblasts were determined. Statistical significance by Mann-Whitney U and Spearman's correlation test was p Daha fazlası Daha az