Çölgeçen H. | Koca Çalişkan U. | Kartal M. | Büyükkartal H.N.

Article | 2014 | Turkish Journal of Biology38 ( 5 ) , pp.619 - 627

The main goal of this study was to establish callus cell suspension cultures of Medicago sativa L. 'Elci' and natural tetraploid Trifolium pratense L. and to compare the isoflavone production of the cultures to the original plants. The callus culture was transferred to liquid Murashige and Skoog media (MS3 and MS5) in order to establish the cell suspension cultures. The extracts were then analyzed by liquid chromatography-mass spectroscopy (LC-MS) for their isoflavones (phytoestrogen), mainly formononetin, biochanin A, daidzein, and genistein. The production of daidzein and formononetin was higher in cell suspension culture than in . . .callus and herba of M. sativa L. 'Elci,' while biochanin A and genistein content could not be detected. On the other hand, the production of phytoestrogens was more successful in the herba of T. pratense L. than in both of the cultures. It might be suggested that T. pratense L. can be grown in larger fields, whereas M. sativa L. can be utilized to establish in vitro cultures in order to produce isoflavone compounds for pharmaceutical purposes. © TÜBİTAK Daha fazlası Daha az

Çölgeçen, Hatice | Çalışkan, Ufuk Koca | Kartal, Murat | Büyükkartal, Hatice Nurhan

Article | 2014 | Turkish Journal of Biology38 ( 5 ) , pp.619 - 627

The main goal of this study was to establish callus cell suspension cultures of Medicago sativa L. 'Elçi' and natural tetraploid Trifolium pratense L. and to compare the isoflavone production of the cultures to the original plants. The callus culture was transferred to liquid Murashige and Skoog media (MS3 and MS5) in order to establish the cell suspension cultures. The extracts were then analyzed by liquid chromatography-mass spectroscopy (LC-MS) for their isoflavones (phytoestrogen), mainly formononetin, biochanin A, daidzein, and genistein. The production of daidzein and formononetin was higher in cell suspension culture t . . .han in callus and herba of M. sativa L. 'Elçi,' while biochanin A and genistein content could not be detected. On the other hand, the production of phytoestrogens was more successful in the herba of T. pratense L. than in both of the cultures. It might be suggested that T. pratense L. can be grown in larger fields, whereas M. sativa L. can be utilized to establish in vitro cultures in order to produce isoflavone compounds for pharmaceutical purposes. The main goal of this study was to establish callus cell suspension cultures of Medicago sativa L. 'Elçi' and natural tetraploid Trifolium pratense L. and to compare the isoflavone production of the cultures to the original plants. The callus culture was transferred to liquid Murashige and Skoog media (MS3 and MS5) in order to establish the cell suspension cultures. The extracts were then analyzed by liquid chromatography-mass spectroscopy (LC-MS) for their isoflavones (phytoestrogen), mainly formononetin, biochanin A, daidzein, and genistein. The production of daidzein and formononetin was higher in cell suspension culture than in callus and herba of M. sativa L. 'Elçi,' while biochanin A and genistein content could not be detected. On the other hand, the production of phytoestrogens was more successful in the herba of T. pratense L. than in both of the cultures. It might be suggested that T. pratense L. can be grown in larger fields, whereas M. sativa L. can be utilized to establish in vitro cultures in order to produce isoflavone compounds for pharmaceutical purposes Daha fazlası Daha az

Çölgeçen, Hatice | Koca, Ufuk | Toker, Gülnur

Article | 2011 | Turkish Journal of Biology35 ( 4 ) , pp.513 - 520

Bu çalışmada sodyum hipoklorit ve Bitki Koruyucu Karışımın yüzey sterilizasyonundaki etkileri araştırılmıştır. Antibiyotik-antimikotik çözeltisinin kültür ortamına eklenerek kallus indüksiyonundaki etkilerini inceledik. Eksplantlar başlangıç olarak farklı sodyum hipoklorit konsantrasyonlarıyla steril edilerek kinetin (0,29 ?M) ve naft alen asetik asit (NAA,10 ?M) içeren MS ortamında kültüre alınmıştır. Eksplantlardaki kontaminasyonlar veya yüksek sodyum hipoklorit konsantrasyonundan dolayı kallus oluşumu gözlenmemiştir. Farklı konsantrasyonlardaki Bitki Koruyucu Karışımı ve antibiyotikler kontaminasyonu azaltmış ve sürgün ucu ve gen . . .ç kök eksplantlarında kallus indüksiyonu gerçekleşmiştir. En iyi kallus oluşumu % 1-2 Bitki Koruyucu Karışım kullanımında bulunurken, sürgün uçlarındaki kallus indüksiyonu yüksek konsantrasyonda (% 4) azalmıştır. Bu çalışma, doğal ortamında arazide yetişen A. densifl ora’nın eksplant kontaminasyonlarının azaltılması ve başarılı sterilizasyon ile ilgili ilk çalışmadır. Oluşturulan kallus kültürleri renkli kalluslar üretti bunlar da spektrofotometrik yöntemle analiz edildi. We analyzed the eff ects of sodium hypochlorite and Plant Preservative Mixture (PPM) on surface sterilization. We also examined the eff ects that the addition of an antibiotic-antimycotic solution to the culture medium had on callus induction. Explants were initially sterilized with diff erent concentrations of sodium hypochlorite and cultured on MS media containing kinetin (0.29 μM) and naphthalene acetic acid (NAA, 10 μM). No calluses were produced, either because of contamination of the explants, or loss of explants as a result of the high levels of sodium hypochlorite. Th e application of PPM and antibiotics at diff erent concentrations reduced contamination and led to callus induction from shoot apexes and young root explants. Th e best callus responses were obtained using PPM at 1%-2%, whereas callus induction on shoot apexes diminished at higher concentrations (4% PPM). Th is is the fi rst report of successful sterilization and reduced contamination of explants from naturally fi eld grown A. densifl ora by PPM. Moreover, established callus cultures produced pigmented calluses, which were analyzed spectrophotometrically Daha fazlası Daha az

Çölgeçen H. | Koca U. | Toker G.

Article | 2011 | Turkish Journal of Biology35 ( 4 ) , pp.513 - 520

We analyzed the effects of sodium hypochlorite and Plant Preservative Mixture (PPM) on surface sterilization. We also examined the effects that the addition of an antibiotic-antimycotic solution to the culture medium had on callus induction. Explants were initially sterilized with different concentrations of sodium hypochlorite and cultured on MS media containing kinetin (0.29 µM) and naphthalene acetic acid (NAA, 10 µM). No calluses were produced, either because of contamination of the explants, or loss of explants as a result of the high levels of sodium hypochlorite. T e application of PPM and antibiotics at different concentrati . . .ons reduced contamination and led to callus induction from shoot apexes and young root explants. T e best callus responses were obtained using PPM at 1%-2%, whereas callus induction on shoot apexes diminished at higher concentrations (4% PPM). This is the first report of successful sterilization and reduced contamination of explants from naturally field grown A. densiflora by PPM. Moreover, established callus cultures produced pigmented calluses, which were analyzed spectrophotometrically. © TÜBİTAK Daha fazlası Daha az

Çoruh, Nursen | Özdoğan, Nizamettin

Article | 2017 | Turkish Journal of Biology41 ( 1 ) , pp.195 - 212

Rosa heckeliana, as one of the wild-growing species of the family Rosacea, has a wide use in the folk medicine; however, scientifically there is very little known about it. Our objective was to examine the antioxidative and antiproliferative properties of Rosa heckeliana root extract and its phenolics. The phenolic constituents, namely catechin, caffeic acid, and ellagic acid, were obtained by the methods of extraction, fractionation, and purification through column chromatography. The DPPH and ABTS radical scavenging assays and total flavonoid content analysis were employed as the current antioxidant methods over the crude extract . . .and fractionated parts. Among the extracts/fractions, the ethyl acetate fraction exhibited the highest amount of flavonoid content (4.58 ± 0.018 µg CE/ mg of extract). The ethyl acetate fraction also displayed high antioxidative properties for DPPH (EC50 value: 2.78 ± 0.01 µg/mL) and ABTS scavenging capacities (586 ± 6.64 µM trolox as TEAC value). Additionally, the antiproliferative properties of the crude extract and the ethyl acetate fraction and its isolated constituents were evaluated for two breast cancer cell lines (MCF-7 and MDA-MB-231) using the XTT method. Acetate fraction over the cells of MCF-7 and MDA-MB-231 resulted in ED50 values of 61.18 ± 0.99 µg/mL and 62.54 ± 2.01 µg/mL, respectively. The isolated phenolic constituents were twice as effective as the ethyl acetate fraction on both MCF-7 and MDA-MB-231 cells. Rosa heckeliana, as one of the wild-growing species of the family Rosacea, has a wide use in the folk medicine; however, scientifically there is very little known about it. Our objective was to examine the antioxidative and antiproliferative properties of Rosa heckeliana root extract and its phenolics. The phenolic constituents, namely catechin, caffeic acid, and ellagic acid, were obtained by the methods of extraction, fractionation, and purification through column chromatography. The DPPH and ABTS radical scavenging assays and total flavonoid content analysis were employed as the current antioxidant methods over the crude extract and fractionated parts. Among the extracts/fractions, the ethyl acetate fraction exhibited the highest amount of flavonoid content (4.58 ± 0.018 µg CE/ mg of extract). The ethyl acetate fraction also displayed high antioxidative properties for DPPH (EC50 value: 2.78 ± 0.01 µg/mL) and ABTS scavenging capacities (586 ± 6.64 µM trolox as TEAC value). Additionally, the antiproliferative properties of the crude extract and the ethyl acetate fraction and its isolated constituents were evaluated for two breast cancer cell lines (MCF-7 and MDA-MB-231) using the XTT method. Acetate fraction over the cells of MCF-7 and MDA-MB-231 resulted in ED50 values of 61.18 ± 0.99 µg/mL and 62.54 ± 2.01 µg/mL, respectively. The isolated phenolic constituents were twice as effective as the ethyl acetate fraction on both MCF-7 and MDA-MB-231 cells Daha fazlası Daha az