Rad A.Y. | Yavuz H. | Kocakulak M. | Denizli A.

Article | 2003 | Macromolecular Bioscience3 ( 9 ) , pp.471 - 476

Bioaffinity separation has a unique and powerful role as a support tool in the removal of toxic substances from human plasma. Magnetic beads have advantages as supports in comparison to conventional nonmagnetic beads because of low pressure drop, high mass transfer rates, and good fluid-solid contact. In addition, they eliminate internal diffusion limitations. Human serum albumin (HSA) immobilised onto magnetic poly(2-hydroxyethyl methacrylate) (mPHEMA) beads were investigated as an adsorbent for the selective bilirubin removal from human plasma. The mPHEMA beads were prepared by a modified suspension polymerisation. HSAwas covalent . . .ly coupled to the mPHEMA beads. Bilirubin adsorption was investigated from hyperbilirubinemic human plasma on the mPHEMA beads containing different amounts of immobilised HSA, (between 11 - 100 mg/g). The nonspecific bilirubin adsorption on the unmodified mPHEMA beads was 0.47 mg/g. Higher bilirubin adsorption capacities, up to 64.7 mg/g, were obtained with the HSA-immobilised magnetic beads. Bilirubin adsorption increased with increasing temperature Daha fazlası Daha az

Arslan H. | Pfaff A. | Lu Y. | Stepanek P. | Müller A.H.E.

Article | 2014 | Macromolecular Bioscience14 ( 1 ) , pp.81 - 91

Çakmaklı, Birten | Hazer, Baki | Tekin, İshak Özel | Kızgut, Sait | Köksal, Murat | Menceloğlu, Yusuf

Article | 2004 | Macromolecular Bioscience4 ( 7 ) , pp.649 - 655

Syntheses of wholly natural polymeric linseed oil (PLO) containing peroxide groups have been reported. Peroxidation, epoxidation and/or perepoxidation reactions of linseed oil, either under air or under oxygen flow at room temperature, resulted in polymeric peroxides, PLO-air and PLO-ofl, containing 1.3 and 3.5 wt.-% of peroxide, with molecular weights of 2 100 and 3 780 Da, respectively. PLO-air contained cross-linked film up to 46.1 wt.-% after a reaction time of 60 d, associated with a waxy, soluble part (PLO-air-s) that was isolated with chloroform extraction. PLO-ofl was obtained as a waxy, viscous liquid without any cross-link . . .ed part at the end of 24 d under visible irradiation and oxygen flow. Polymeric peroxides, PLO-air-s and PLO-ofl initiated the free radical polymerization of both methyl methacrylate (MMA) and styrene (S) to give PMMA-graft-PLO and PS-graft-PLO graft copolymers in high yields with Mw varying from 37 to 470 kDa. The polymers obtained were characterized by FT-IR, 1H NMR, TGA, DSC and GPC techniques. Cross-linked polymers were also studied by means of swelling measurements. PMMA-graft-PLO graft copolymer film samples were also used in cell-culture studies. Fibroblast cells were well adhered and proliferated on the copolymer film surfaces, which is important in tissue engineering Daha fazlası Daha az