The effect of anthelmintic triclabendazole on survival and development of Galleria mellonella (Lepidoptera: Pyralidae) L. reared on artificial diet.

Kılıç, Ali | Büyükgüzel, Kemal | Büyükgüzel, Ender


The effect of triclabendazole, which is a benzimidazole anthelmintic, on the survival rate and developmental time in different stages of greater wax moth Galleria mellonella (L.) was investigated by rearing the larvae on the artificial diets. Triclabendazole was incorporated into diets at concentrations of 0.001, 0.01 or 0.1%. G. mellonella larvae was reared from first instar larvae to adult emergence on the artificial diets with different concentrations of triclabendazole. The survival rate in seventh instar (7th-instar) and adult stage were significantly lower at low concentrations of this anthelmintic agent (0.001 and 0.01 g per . . .100 g of diet) than the control, while there were no differences on pupation in comparison to control diet. However, the pupation and adult emergence were significantly lower at the highest dietary concentration of triclabendazole than control group but there were no differences on survival of 7th-instar larvae. The highest concentration of the anthelmintic agent (0.1 g/100 g diet) decreased pupation from 81.6 +/- 4.32% to 43.3 +/- 7.45%, adult emergence rate from 73.3 +/- 2.36 to 13.3 +/- 2.36. Triclabendazole at the highest concentration prolonged developmental time to 7th larval stage by 3.8 days reaching total 22.8 +/- 1.54 days. This diet containing 0.1% of triclabendazole significantly prolonged pupal developmental time from 24.6 +/- 1.24 days to 28.4 +/- 1.24 days, adult developmental time from 35.3 +/- 1.27 days to 42.0 +/- 1.62 days. This study is of importance in appreciation for usage of antihelmintic with different structure and mode of actions in the management of pest insects to reduce damage to environment and nontarget organisms Daha fazlası Daha az

The effect of gemifloxacin on some biological traits of galleria mellonella (lepidoptera: pyralidae) adults

Hız, Pınar | Erdem, Meltem | Büyükgüzel, Ender | Büyükgüzel, Kemal


Greater wax moth, Galleria mellonella L. is most commonly used as model insects for studying insecticidal efficiency of chemicals as well as it has been known as an alternative model to mamalian model for studying microbial infections and clinical drugs. The effects of a fluoroquinolone antibacterial agent, gemifloxacin, on sex ratio, male and female adult longevity, fecundity and hatchability of this insect were investigated by rearing the first instar larvae on artificial diets in the laboratory condition. The insect was reared from first-instar larvae to adult stage on an artificial diets containing gemifloxacin at 0.001, 0.01, 0 . . ..1 or 1.0%. Gemifloxacin did not affect male and female sex ratio and adult longevity while it significantly decreased egg number. Fecundity and hatchability were significantly and inversely decreased by increasing gemifloxacin concentrations. The females reared from control diet produced 134.46 per day, whereas high concentrations of gemifloxacin decreased the egg number to 26.75 and 53.5. Hatchability was also significantly decreased by 0.01% and above concentrations of gemifloxacin whereas, the highest concentration of antibiotic lowered the hatchability to 53.71%. This study indicated sublethal effects of gemifloxacin are likely to have a significant impact on adult biological traits and these effects show concentration-dependent variation in biological traits of the insect Daha fazlası Daha az

More than an Uterotonic Agent: Oxytocin Prevents Peritoneal Adhesion

Isik, Hatice | Sahbaz, Ahmet | Aynioglu, Oner | Ozmen, Ulku Bayar | Cengil, Osman | Dogan Gun, Banu


Prevention of postoperative adhesions (PPA) has become an important issue. The aim is to investigate the effect of Oxytocin (OT) on PPAs. A total of thirty female Wistar-albino rats were randomly divided into three groups (10 rats/group). The cecal peritone of Group I rats (controls) were scraped, to trigger adhesion formation, and no treatment were given. After cecal scrubbing, 1 mL saline solution was applied to each rat in Group II (i.p. saline treated group) and 80 IU/kg of OT (Pituisan (R), Ege Vet, Turkey) to Group III (i.p. OT treated group) intraperitoneally. All animals were sacrificed 10 days after surgery and adhesions gr . . .aded in terms of severity and histopathologic characteristics. The median scores for the extent, severity, and degree of adhesions in Group I and Group II were statistically significant and considerably higher than those scores for Group III ( Daha fazlası Daha az

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