Büyükgüzel, Ender | Kalender, Yusuf

Article | 2008 | Journal of Entomological Science43 ( 1 ) , pp.27 - 40

Antibiotics are routinely incorporated in insect culture media. Although culturing insects on diets containing antibiotics is a decades-old practice, the antibiotics can exert deleterious effects on the insects. Diets amended with penicillin, streptomycin, fluconazole or griseofulvin were evaluated as to their impact on survivorship, development, wet weight, and adult total protein content of Galleria mellonella (L.). Insects were reared from neonates to adults on artificial diets containing 0.001, 0.01, 0.1 or 1.0 g of the antibiotics (per 100 g diet). Dose- and stage-dependent variations in both biological and biochemical paramete . . .rs occurred. Penicillin at high concentrations significantly increased the wet weight of the insect, whereas low dietary fluconazole, griseofulvin and streptomycin concentrations significantly increased wet weight and high concentrations decreased wet weight. Dietary antibiotic treatment resulted in significantly decreased survivorship and increased developmental time of larvae. The diet amended with 1.0 g of either penicillin or streptomycin decreased pupation and adult emergence by 50%. Larvae reared on the diets supplemented with the highest concentrations of fluconazole and griseofulvin produced as low as 20% of adults. The 0.1 g fluconazole treatment prolonged adult development by 8 d. High dietary griseofulvin concentrations markedly decreased total protein content of adults. Other antibiotics also resulted in decreased total protein content in adults depending on their types and concentrations. Slightly enhanced survivorship, shortened development and increased total protein content were observed with some sublethal doses of antibiotics. It appears that dietary antibiotic impact on insect biological parameters is exerted via their deteriorative effects on biochemical factors in relation to alterations in wet weight. Low concentrations of these antibiotics can be used in artificial rearing of G. mellonella Daha fazlası Daha az

Büyükgüzel, Ender

Article | 2014 | Journal of Entomological Science49 ( 2 ) , pp.144 - 155

Eicosandoids, or icosanoids, are signaling compounds created by the oxidation of 20-carbon fatty acids. They control many complex physiological and immunological functions in vertebrate and invertebrate animals. This study tested the hypothesis that eicosanoids act in insect antioxidant defense. The effects of 3 eicosanoid biosynthesis inhibitors (EBIs) - dexamethasone, esculetin, and phenidone - on the oxidative stress indicator, malondialdehyde (MDA), and the detoxification enzyme, glutathione S-transferase (GST), was examined in the midgut of larvae of the greater wax moth, Galleria mellonella (L). The larvae were reared on artif . . .icial diets supplemented with 0.001, 0.01, 0.1 or 1.0% of the EBIs. Esculetin, which is a lipoxygenase inhibitor, significantly increased MDA content; whereas, GST activity was significantly increased at only the highest concentration tested. Dexamethasone, a phospholipase A2 inhibitor, significantly increased MDA content and GST activity at concentrations of 0.01, 0.1, and 1.0%. Phenidone, a dual inhibitor of cyclooxygenase and lipoxygenase, increased MDA content, whereas the 0.01 and 0.1% concentrations of phenidone significantly increased GST activity. Our results indicate that antioxidative responses are, at least in part, controlled by a physiological system that includes eicosanoid biosynthesis Daha fazlası Daha az

Büyükgüzel, Kemal | İçen, Ender

Article | 2004 | Journal of Entomological Science39 ( 1 ) , pp.108 - 116

The effects of antibiotics, novobiocin, nalidixic acid and oxolinic acid, singly and in combinations, on the wet weight and total protein content of the hymenopterous endoparasitoid, Pimpla turionellae L., were investigated by rearing the larvae aseptically on chemically defined synthetic diets. Oxolinic acid, at all levels tested, caused a greater increase in the total protein content of the young pupae than other antibiotics; whereas, 2.25 mg of this agent decreased both the wet weight and total protein of adults. However, this antibiotic did not affect the pupal wet weight. High levels of novobiocin increased the protein content . . .of the pupae but had no effect on that of the adults. The lowest level of this antibiotic significantly increased pupal and adult wet weight. While this level of antibiotic had no effect on pupal protein content, it significantly increased the protein content of adults. The highest tested level of nalidixic acid caused a significant decrease in the pupal and adult wet weight of the insect. This level increased total protein content of the pupae. None of the tested levels of this antibiotic had an effect on the protein content of the adults. The combinations with two antibiotics had no significant effects on the total protein content, while some increased the wet weight of the insect. Some ternary combinations of novobiocin, nalidixic acid and oxolinic acid (6.0:2.0:0.75, 6.0:2.0:3,0, and 1.5: 4.0:0.75 mg) significantly increased the total protein content in adults of the insect, and the combination of 6.0:2.0:0.75 increased the protein content and wet weight of pupae. It is suggested that these three antibiotics singly and in combination can be used in artificial rearing of this parasitoid Daha fazlası Daha az

Büyükgüzel, Ender | Büyükgüzel, Kemal

Article | 2019 | Journal of Entomological Science54 ( 1 ) , pp.79 - 86

Galleria mellonella L. larvae were reared on a standard artificial diet amended with various concentrations (0.5%, 1.0%, 1.5%, and 2.0%) of the aminocoumarin DNA gyrase inhibitor antibiotic drug novobiocin. The effects of the inhibitor on survival and development of the larvae were measured. Survival rates for 7th instars, pupae, and emerging adults were 90.0%, 86.2%, and 76.2%, respectively, in the control diet. These rates were decreased to 15.0%, 7.5%, and 6.2% for larvae fed the diet with the highest novobiocin concentration (2.0%). High concentrations of the antibiotic caused prolongation in the larval, pupal, and adult develop . . .mental periods. Our results indicate that this gyrase inhibitor can be used as dietary additive in artificial rearing of G. mellonella when its concentrations are ascertained. © 2019 Georgia Entomological Society Inc. All rights reserved Daha fazlası Daha az

Sugeçti, Serkan | Büyükgüzel, Ender | Büyükgüzel, Kemal

Article | 2016 | Journal of Entomological Science51 ( 2 ) , pp.129 - 137

Galleria mellonella L. larvae were reared on a standard diet amended with varying concentrations (0, 0.0015, 0.015, 0.15, and 1.5%) of the benzimidazole-derivative antihelmintic drug oxfendazole. Survivorship, developmental times, longevity, fecundity, and fertility were monitored over the treatments. Relative to the untreated control, exposure to diet containing 1.5% oxfendazole significantly decreased survivorship in larval, pupal, and adult stages, prolonged the time to reach the adult stage, and reduced adult longevity. Oxfendazole at all concentrations significantly lowered egg production per female and decreased egg hatch. The . . .se results demonstrate that this antihelmintic may be explored as a candidate for insect pest control Daha fazlası Daha az

Erdem, Meltem | Büyükgüzel, Ender | Büyükgüzel, Kemal

Article | 2016 | Journal of Entomological Science51 ( 4 ) , pp.305 - 313

he fruit fly Drosophila melanogaster (Meigen) (Diptera: Drosophilidae) is often used in various biological, molecular, and toxicological studies. Sodium tetraborate, a boron compound, was added to the artificial diet of developing D. melanogaster to determine its effects on adult longevity and fecundity. Insects reared from first instars to adults on diets containing 10, 30, 150, 300, or 400 mg/liter sodium tetraborate showed that the highest concentration (400 mg/liter) significantly reduced female longevity (31.65 6 4.02 [mean 6 SD] d for the controls versus 1.87 6 0.30 d for 400 mg/liter) and male longevity (32.80 6 1.96 d for co . . .ntrols versus 3.57 6 0.42 d for 400 mg/liter). Females from the control diet produced 9.46 6 0.57 (mean 6 SD) eggs per female, whereas those fed on a diet containing 300 mg/liter produced only 1.92 6 0.30 eggs per females. These results expand our knowledge of the impact of sodium tetraborate on various insects and indicate that boron compounds should be further investigated to ascertain their potential as an alternative control tactic for pest insects Daha fazlası Daha az