İlhan, Elif | Karahaliloğlu, Zeynep | Kılıçay, Ebru | Hazer, Baki | Denkbaş, Emir Baki

Article | 2019 | Materials Technology

Postoperative infection in orthopaedic and trauma surgery is one of the most feared complications. Recently, the high prevalence of multidrug-resistant bacteria has made the antibiotic treatment ineffective; thus novel non-antibiotic alternative approaches to this problem are urgently needed. Based on these expectations, in this work, in order to enhance the cytocompatibility and antibacterial performances of poly (methyl methacrylate (PMMA) and beta-tricalcium phosphate (ß-TCP) bone cements were impregnated with polystyrene (PS)-g-soybean oil graft copolymer containing AgNPs (PS-Agsbox), and we assessed the antimicrobial activity o . . .f the fabricated bone cements against Staphylococcus aureus and Escherichia coli. Nanoparticles at concentration of 1.25% (5 ß-TCP) w/w in ß-TCP bone cements were able to inhibit pathogens growth, while a concentration of 3.75% (15PMMA) was needed for PMMA bone cement. Therefore, the impregnated bone cements with PS-AgsboxNPs may be further explored as an alternative antimicrobial therapy for the treatment of infected bone defects. © 2019, © 2019 Informa UK Limited, trading as Taylor & Francis Group Daha fazlası Daha az

Kılıçay, Ebru | Karahaliloğlu, Zeynep | Alpaslan, Pınar | Hazer, Baki | Denkbaş, Emir Baki

Article | 2017 | JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION28 ( 15 ) , pp.1762 - 1785

Antisense oligonucleotide (ASO)-conjugated--tocopherol succinate (TCS)-loaded-poly(lactic acid)-g-poly(ethylene glycol) nanoparticles (ASO-TCS-PLA-PEG NPs), with the ratio of polymer/TCS of 10:2.5, 10:5, 10:7 (w/w) were prepared for targeting cancer therapy. The amphiphilic PLA, amino terminated PEG graft copolymers were synthesized by ring opening polymerization reaction. Nanoparticles were produced by using double emulsion (w/o/w) solvent evaporation method. ASO-TCS-PLA-PEG NPs demonstrated satisfactory encapsulation and loading efficiency and size distribution. The short-term stability studies were carried out at 4 and 25 degrees . . . C for 30days to assess their mean particle size, polydispersity index and zeta potential. The cellular uptake and extended cytoplasmic retention of the NPs in A549 human lung carcinoma and L929 mouse fibroblast cells were examined by fluorescence and confocal microscopy. In human lung cancer cells, ASO-TCS-PLA-PEG NPs exhibited better cellular internalization, cytotoxicity and apoptotic and necrotic effects compared to healthy cell line, L929. These findings showed that ASO-modified nanoparticles could serve as a promising nanocarrier for targeted tumor cells Daha fazlası Daha az

Kılıçay, Ebru | Erdal, Ebru | Hazer, Baki | Türk, Mustafa | Denkbaş, Emir Baki

Article | 2016 | Artificial Cells, Nanomedicine and Biotechnology44 ( 8 ) , pp.1938 - 1948

Amphiphilic poly(3-hydroxylalkanoate) (PHA) copolymers find interesting applications in drug delivery. The aim of this study was to prepare nucleic acid adsorbed on (PHB-b-PEG-NH2) nanoparticle platform for gene delivery. For this purpose, PHB-b-PEG-NH2 block copolymers were synthesized via transesterification reactions. The copolymers obtained were characterized by Proton Nuclear Magnetic Resonance (1H-NMR), Fourier Transform Infrared Spectrometer (FTIR), Thermogravimetric Analysis (TGA) and Differential Scanning Calorimetry (DSC) techniques. The cytotoxic, apoptotic and necrotic effects of these nanoparticles in the MDA 231 human . . .breast cancer cell, the A549 human lung cancer cell and the L929 fibroblast cell lines were also investigated. © 2015 Taylor & Francis Daha fazlası Daha az

Kılıçay, Ebru | Karahaliloğlu, Zeynep | Hazer, Baki | Tekin, İshak Özel | Denkbaş, Emir Baki

Article | 2016 | Journal of Microencapsulation33 ( 3 ) , pp.274 - 285

The aim of this study was to evaluate therapeutic potential of curcumin-loaded poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) PHBHHx nanoparticles (CUR-NPs) and concanavaline A conjugated curcumin-loaded NPs (ConA-CUR-NPs) for breast cancer treatment. The size and zeta potential of prepared NPs were about 228 ± 5 nm and -23.3 mV, respectively. The entrapment efficiencies of polymer/drug weight ratios, 1.25CUR-NPs, 2.5CUR-NPs, 5CUR-NPs, ConA-1.25CUR-NPs, ConA-2.5CUR-NPs and ConA-5CUR-NPs were found to be ?68, 55, 45, 70, 60 and 51%, respectively. Optimized NPs formulations in the freeze-dried form were assessed with their short-term s . . .tability for 30 days of storage at 4 °C and 25 °C. Anticancer activity of ConA-CUR-NPs was proved by MTT assay and reconfirmed by double staining and flow cytometry results. The anticancer activity of ConA-CUR-NPs was measured in human breast cancer cells (MDA-MB 231) in vitro, and the results revealed that the ConA-CUR-NPs had better tumor cells decline activity. © 2016 Informa UK Limited, trading as Taylor & Francis Group Daha fazlası Daha az

Anılmış, Nur Merve | Kara, Göknur | Kılıçay, Ebru | Hazer, Baki | Denkbaş, Emir Baki

Article | 2019 | Journal of Microencapsulation36 ( 7 ) , pp.635 - 648

In this study, the anticancer activities of two siRNA carriers were compared using a human lung adenocarcinoma epithelial cell line (A549). Firstly, poly(styrene)-graft-poly(linoleic acid) (PS-g-PLina) and poly(styrene)-graft-poly(linoleic acid)-graft-poly(ethylene glycol) (PS-g-PLina-g-PEG) graft copolymers were synthesized by free-radical polymerization. PS-PLina and PS-PLina-PEG nanoparticles (NPs) were prepared by solvent evaporation method and were then characterized. The size was found as 150 ± 10 nm for PS-PLina and 184 ± 6 nm for PS-PLina-PEG NPs. The NPs were functionalized with poly(l-lysine) (PLL) for c-myc siRNA conjugat . . .ion. siRNA entrapment efficiencies were found in the range of 4–63% for PS-PLina-PLL and 6–42% for PS-PLina-PEG-PLL NPs. The short-term stability test was realised for 1 month. siRNA release profiles were also investigated. In vitro anticancer activity of siRNA-NPs was determined by MTT, flow cytometry, and fluorescence microscopy analyses. Obtained findings showed that both NPs systems were promising as siRNA delivery tool for lung cancer therapy. © 2019, © 2019 Informa UK Limited, trading as Taylor & Francis Group Daha fazlası Daha az

Karahaliloğlu, Zeynep | Kılıçay, Ebru | Alpaslan, Pınar | Hazer, Baki | Denkbaş, Emir Baki

Article | 2018 | JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS33 ( 1 ) , pp.38 - 62

The development of novel combination anticancer drug delivery systems is an important step to improve the effectiveness of anticancer treatment in metastatic breast cancer and to overcome increased toxicity of the currently used combination treatments. The aim of this study was to assess efficient targeting, therapeutic efficacy, and bioavailability of a combination of drugs (curcumin and -tocopheryl succinate) loaded polystyrene-polysoyaoil-diethanol amine nanoparticles. Polystyrene-polysoyaoil-diethanol amine nanoparticles encapsulating two drugs, individually or in combination, were prepared by double-emulsion solvent evaporation . . . method, resulting in particle size smaller than 250nm with a surface negative charge between -30 and -40mV. Entrapment efficiency of curcumin and -tocopheryl succinate in the epigallocatechin gallate-conjugated dual-drug-loaded nanoparticles was found to be 68% and 80%, respectively. The release kinetics of curcumin and -tocopheryl succinate from the nanoparticles exhibited a gradual and continuous profile followed by an initial burst behavior with a release over 20days in vitro. Next, we have investigated the anticancer activity of nanoparticles encapsulating both the drugs and individually drug in human breast cancer cells (MDA-MB-231) using double-staining-based cell death analysis, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assessment of cytotoxicity and flow cytometer. In vitro cytotoxicity studies revealed that epigallocatechin gallate--tocopheryl succinate/curcumin-polystyrene-polysoyaoil-diethanol amine nanoparticles are more potent than the corresponding -tocopheryl succinate/curcumin-polystyrene-polysoyaoil-diethanol amine nanoparticles and their single-drug-loaded forms and show a synergistic and breast tumor targeting function. Thus, here, we propose epigallocatechin gallate-conjugated curcumin and -tocopheryl succinate-loaded polystyrene-polysoyaoil-diethanol amine nanoparticles which effectively inhibit tumor growth and reduce toxicity compared to single-drug chemotherapy Daha fazlası Daha az