İlhan, Elif | Karahaliloğlu, Zeynep | Kılıçay, Ebru | Hazer, Baki | Denkbaş, Emir Baki

Article | 2019 | Materials Technology

Postoperative infection in orthopaedic and trauma surgery is one of the most feared complications. Recently, the high prevalence of multidrug-resistant bacteria has made the antibiotic treatment ineffective; thus novel non-antibiotic alternative approaches to this problem are urgently needed. Based on these expectations, in this work, in order to enhance the cytocompatibility and antibacterial performances of poly (methyl methacrylate (PMMA) and beta-tricalcium phosphate (ß-TCP) bone cements were impregnated with polystyrene (PS)-g-soybean oil graft copolymer containing AgNPs (PS-Agsbox), and we assessed the antimicrobial activity o . . .f the fabricated bone cements against Staphylococcus aureus and Escherichia coli. Nanoparticles at concentration of 1.25% (5 ß-TCP) w/w in ß-TCP bone cements were able to inhibit pathogens growth, while a concentration of 3.75% (15PMMA) was needed for PMMA bone cement. Therefore, the impregnated bone cements with PS-AgsboxNPs may be further explored as an alternative antimicrobial therapy for the treatment of infected bone defects. © 2019, © 2019 Informa UK Limited, trading as Taylor & Francis Group Daha fazlası Daha az

Karahaliloğlu, Zeynep | Kılıçay, Ebru | Alpaslan, Pınar | Hazer, Baki | Denkbaş, Emir Baki

Article | 2018 | JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS33 ( 1 ) , pp.38 - 62

The development of novel combination anticancer drug delivery systems is an important step to improve the effectiveness of anticancer treatment in metastatic breast cancer and to overcome increased toxicity of the currently used combination treatments. The aim of this study was to assess efficient targeting, therapeutic efficacy, and bioavailability of a combination of drugs (curcumin and -tocopheryl succinate) loaded polystyrene-polysoyaoil-diethanol amine nanoparticles. Polystyrene-polysoyaoil-diethanol amine nanoparticles encapsulating two drugs, individually or in combination, were prepared by double-emulsion solvent evaporation . . . method, resulting in particle size smaller than 250nm with a surface negative charge between -30 and -40mV. Entrapment efficiency of curcumin and -tocopheryl succinate in the epigallocatechin gallate-conjugated dual-drug-loaded nanoparticles was found to be 68% and 80%, respectively. The release kinetics of curcumin and -tocopheryl succinate from the nanoparticles exhibited a gradual and continuous profile followed by an initial burst behavior with a release over 20days in vitro. Next, we have investigated the anticancer activity of nanoparticles encapsulating both the drugs and individually drug in human breast cancer cells (MDA-MB-231) using double-staining-based cell death analysis, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assessment of cytotoxicity and flow cytometer. In vitro cytotoxicity studies revealed that epigallocatechin gallate--tocopheryl succinate/curcumin-polystyrene-polysoyaoil-diethanol amine nanoparticles are more potent than the corresponding -tocopheryl succinate/curcumin-polystyrene-polysoyaoil-diethanol amine nanoparticles and their single-drug-loaded forms and show a synergistic and breast tumor targeting function. Thus, here, we propose epigallocatechin gallate-conjugated curcumin and -tocopheryl succinate-loaded polystyrene-polysoyaoil-diethanol amine nanoparticles which effectively inhibit tumor growth and reduce toxicity compared to single-drug chemotherapy Daha fazlası Daha az