Kılıçay, Ebru | Erdal, Ebru | Hazer, Baki | Türk, Mustafa | Denkbaş, Emir Baki

Article | 2016 | Artificial Cells, Nanomedicine and Biotechnology44 ( 8 ) , pp.1938 - 1948

Amphiphilic poly(3-hydroxylalkanoate) (PHA) copolymers find interesting applications in drug delivery. The aim of this study was to prepare nucleic acid adsorbed on (PHB-b-PEG-NH2) nanoparticle platform for gene delivery. For this purpose, PHB-b-PEG-NH2 block copolymers were synthesized via transesterification reactions. The copolymers obtained were characterized by Proton Nuclear Magnetic Resonance (1H-NMR), Fourier Transform Infrared Spectrometer (FTIR), Thermogravimetric Analysis (TGA) and Differential Scanning Calorimetry (DSC) techniques. The cytotoxic, apoptotic and necrotic effects of these nanoparticles in the MDA 231 human . . .breast cancer cell, the A549 human lung cancer cell and the L929 fibroblast cell lines were also investigated. © 2015 Taylor & Francis Daha fazlası Daha az

Kılıçay, Ebru | Karahaliloğlu, Zeynep | Hazer, Baki | Tekin, İshak Özel | Denkbaş, Emir Baki

Article | 2016 | Journal of Microencapsulation33 ( 3 ) , pp.274 - 285

The aim of this study was to evaluate therapeutic potential of curcumin-loaded poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) PHBHHx nanoparticles (CUR-NPs) and concanavaline A conjugated curcumin-loaded NPs (ConA-CUR-NPs) for breast cancer treatment. The size and zeta potential of prepared NPs were about 228 ± 5 nm and -23.3 mV, respectively. The entrapment efficiencies of polymer/drug weight ratios, 1.25CUR-NPs, 2.5CUR-NPs, 5CUR-NPs, ConA-1.25CUR-NPs, ConA-2.5CUR-NPs and ConA-5CUR-NPs were found to be ?68, 55, 45, 70, 60 and 51%, respectively. Optimized NPs formulations in the freeze-dried form were assessed with their short-term s . . .tability for 30 days of storage at 4 °C and 25 °C. Anticancer activity of ConA-CUR-NPs was proved by MTT assay and reconfirmed by double staining and flow cytometry results. The anticancer activity of ConA-CUR-NPs was measured in human breast cancer cells (MDA-MB 231) in vitro, and the results revealed that the ConA-CUR-NPs had better tumor cells decline activity. © 2016 Informa UK Limited, trading as Taylor & Francis Group Daha fazlası Daha az