Arama Sonuçları

Molecular Survey of Babesia microti (Aconoidasida: Piroplasmida) in Wild Rodents in Turkey

Review | 2019 | Journal of Medical Entomology56 ( 6 ) , pp.1605 - 1609

Babesia microti (Aconoidasida: Piroplasmida) (Franca, 1910) is an important tick-borne zoonotic parasite with rodents serving as reservoir hosts. In the present study, 536 rodents were captured from Burdur, Bartin, Giresun, and Yozgat provinces of Turkey between the years 2010 and 2012, and blood samples were examined for the presence of Babesia spp. using conventional PCR which targeted the 18S rRNA gene. The sequence analysis of PCR amplicons was tested for B. microti as well as for Hepatozoon spp., and Sarcocystis spp. Overall, 5.8% of the rodents were positive for B. microti: 41% in Myodes glareolus, 7.7% in Chionomys roberti, a . . .nd 2% in Apodemus spp., whereas no Babesia DNA was detected in Mus macedonicus and Microtus spp. Six rodents were positive for Hepatozoon spp. and one rodent was positive for Sarcocystis spp. Overall, 14.9 and 4.5% of rodents captured from Bartin and Giresun provinces, respectively, were PCR positive for B. microti, whereas none of rodents captured in Burdur and Yozgat were positive for Babesia spp. The sequence data of B. microti from rodents revealed that all sequences belonged to the zoonotic genotype. Sequences of B. microti obtained from rodents of the Bartin province were genotypically closer to European isolates, whereas those obtained from rodents of the Giresun province were closer to Russian and Mongolian isolates. © 2019 Crown copyright 2019 Daha fazlası Daha az

Discrimination of 2n=60 Spalax leucodon cytotypes (Spalacidae, Rodentia) in Turkey by means of classical and molecular cytogenetic techniques

Ivanitskaya, E. | Sözen, Mustafa | Rashkovetsky, L. | Matur, Ferhat | Nevo, E.

Article | 2008 | CYTOGENETIC AND GENOME RESEARCH122 ( 2 ) , pp.139 - 149

Comparative studies among seven populations of 2n = 60 S. leucodon employing classic cytogenetics (G-bands, C-bands, AgNOR-staining), fluorochrome staining, and fluorescence in situ hybridization of telomeric and rDNA probes are reported here for the first time. The studied specimens were assigned to two cytotypes: 2n = 60W and 2n = 60R. The basic karyotype of both cytotypes consisted of eight pairs of subtelocentric and 21 pairs of acrocentric autosomes, subtelocentric X and acrocentric Y chromosomes. Both cytotypes had variable numbers of B-chromosomes (1-3) and variable numbers of autosomal arms (NFa = 74-76) caused by amplificat . . .ion (deletion) of heterochromatin short arms in the second pair. The short arms of subtelocentric chromosomes were comprised of heterochromatin in both cytotypes. Nucleolar organizer regions (NORs) and rDNA clusters were detected at telomeric sites of the short arms in pairs Nos. 3, 5, 6, 9, and 13 in cytotype W, and in the short arms of pair No. 6, 8, 12, 13, and 16 in cytotype R. Different locations of rDNA clusters allowed unambiguous discrimination between two S. leucodon cytotypes possessing the same 2n = 60 and similar NFa (74-76) variability. Our findings suggest a high level of chromosomal divergence, which means that it is possible to consider these cytotypes as a well-differentiated, chromosomal lineage within the leucodon group. Copyright (C) 2008 S. Karger AG, Base Daha fazlası Daha az

Dobrava hantavirus variants found in Apodemus flavicollis mice in Kırklareli Province, Turkey

Article | 2018 | Journal of Medical Virology90 ( 5 ) , pp.810 - 818

Hantaviruses infect humans via inhalation of viral particles within secretions of infected rodents or rarely through direct contact with infected rodents. Determining the prevalence of hantavirus infections among rodent populations is of vital importance to obtain information on hantavirus-related cases and to predict possible outbreaks. We hypothesized that DOBV strains circulating in the Thrace Region in Turkey would be related to other Balkan DOBV strains. In this study, hantavirus infections in the rodent population of the Kırklareli-İğneada Region (north-western Turkey, near the Bulgarian border) were investigated. This region . . .is of particular importance, as it is located in the south-eastern margin of the European continent and was used as an entrance point of Asian faunal elements into Europe. DOBV infection was detected in eight of 73 rodents; all were of the Apodemus flavicollis species. Partial sequences of the viral S-, M-, and L-genome segments were recovered and compared with previously reported DOBV sequences. The newly characterized Turkish strains were similar to other DOBV variants. Silent nucleotide mutations were dominant. The hantavirus prevalence in the İğneada region was similar to what has been reported in Greece and Bulgaria. For the first time, the M-segment sequences of DOBV from Turkey were recovered and genetic data of hantaviruses from Thrace region of Turkey were obtained. © 2018 Wiley Periodicals, Inc Daha fazlası Daha az

Data to the cranial and tooth development of Glis glis orientalis nehring, 1903 (rodentia: gliridae)

Çolak, Ercüment | Yiğit, Nuri | Sözen, Mustafa | Özkurt, Şakir

Proceedings | 2003 | Acta Zoologica Academiae Scientiarum Hungaricae49 ( SUPPL. 1 ) , pp.33 - 38

Thirty specimens of Glis glis orientalis born in captivity were used in this study. In captivity, animals were fed on hazelnuts, chestnut, apple, biscuit, acorn and sunflower seeds under uncontrolled conditions. Cranial and dentition features were examined from 45 days to 1824 days. At the age of 45 days, teeth were not worn, the cusp pattern of P4 was not recognisable, and the cusps of M3 and M3 had not erupted from dentary. In 126 days old, cusp line of M3 and M3 reached M2 and M2, and the cusp of P4 had developed. In 186 days old, tooth had started to wear.