Hyršl, Pavel | Büyükgüzel, Ender | Büyükgüzel, Kemal

Article | 2007 | Archives of Insect Biochemistry and Physiology66 ( 1 ) , pp.23 - 31

Larvae of the wax moth, Galleria mellonella (L.), were reared from first instar on a diet supplemented with 156, 620, 1,250, or 2,500 ppm boric acid (BA). The content of malondialdehyde (MDA, an oxidative stress indicator), and activities of the antioxidant enzymes [superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), and glutathione peroxidase (GPx)] were determined in the fat body and hemolymph in the 7th instar larvae and newly emerged pupae. Relative to control larvae, MDA was significantly increased in larval hemolymph, larval and pupal fat body, but decreased in the pupal hemolymph. Insects reared on di . . .ets with 156-and 620-ppm BA doses yielded increased SOD activity but 1,250- and 2,500-ppm doses resulted in decreased SOD activity in larval hemolymph. SOD activity was significantly increased but CAT was decreased in the larval fat body. High dietary BA treatments led to significantly decreased GST activity. However, they increased GPx activity in larval hemolymph. Dietary BA also affected larval survival. The 1,250- and 2,500-ppm concentrations led to significantly increased larval and pupal mortality and prolonged development. In contrast, the lowest BA concentration increased longevity and shortened development. We infer that BA toxicity is related, at least in part, to oxidative stress management. © 2007 Wiley-Liss, Inc Daha fazlası Daha az

Büyükgüzel, Ender | Büyükgüzel, Kemal | Snela, Milena | Erdem, Meltem | Radtke, Katarzyna | Ziemnicki, Kazimierz | Adamski, Zbigniew

Article | 2013 | Cell Biology and Toxicology29 ( 2 ) , pp.117 - 129

Boric acid is widely used as an insecticide, acaricide, herbicide, and fungicide and also during various industrial processings. Hence, numerous populations are subjects to this toxic compound. Its action on animals is still not fully known and understood. We examined the effect of boric acid on larvae of greater wax moth (Galleria mellonella). The chemical appeared to be toxic for larvae, usually in a concentration-dependent manner. Exposed groups revealed increased lipid peroxidation and altered activity of catalase, superoxide dismutase, glutathione S-transferase, and glutathione peroxidase. We also observed changes of ultrastruc . . .ture, which were in tune with biochemical assays. We suggest that boric acid has a broad mode of action, which may affect exposed larvae, and even if sublethal, they may lead to disturbances within exposed populations. © 2013 The Author(s) Daha fazlası Daha az

Güneş, Eda | Büyükgüzel, Ender

Article | 2017 | TURKIYE ENTOMOLOJI DERGISI-TURKISH JOURNAL OF ENTOMOLOGY41 ( 1 ) , pp.3 - 15

Synthetic organic insecticides are widely used to combat agricultural pests. Boric acid has a great importance in pest management because it has less toxic effect on non-target organisms compared to other organic chemical insecticides. For this purpose, the fruit fly Drosophila melanogaster Meigen, 1830 (Diptera: Drosophilidae) was reared from first stage larvae on an artificial diet containing boric acid at 10, 100, 200 or 300 mg/L to adult stage. The effect of boric acid on important oxidative stress indicators such as lipid peroxidation product of malondialdehyde contents (MDA) and protein oxidation products of protein carbonyl c . . .ontents (PCO) and detoxification enzyme activity of glutathione S-transferase (GST) in the third stage larvae, pupae, adults and eggs of D. melanogaster were investigated. All boric acid concentrations significantly increased MDA content in third stage larva: When the adults from the larvae reared on 300 mg/L of dietary BA were also fed with high BA concentration for a 10-day period, MDA and PCO contents of male and female adults were considerably went up in comparison to control. MDA and PCO content in the eggs of these females were hugely increased. The rise in PCO content of the eggs was 31-fold relative to control. Our results indicate that BA feeding at high concentrations in all developmental stages of D. melanogaster is more effective on oxidative stress indicators and detoxification enzyme. Tarımsal zararlılar ile mücadelede sentetik organik insektisitler yoğun olarak kullanılmaktadır. Borik asit organik kimyasal insektisitlere göre hedef olmayan organizmalara karşı daha düşük toksisiteye sahip olması nedeniyle önem taşımaktadır. Bu amaçla çalışmamızda meyve sineği Drosophila melanogaster Meigen,1830 (Diptera: Drosophilidae)'in birinci dönem larvaları borik asitin farklı konsantrasyonlarını (10, 100, 200 ve 300 mg/L) içeren yapay besinler ile yetiştirilmiştir. Drosophila melanogaster' in üçüncü dönem larva, pupa, ergin dönemleri ve yumurtalarında oksidatif stresin önemli indikatörleri olan lipid peroksidasyonu ürünü malondialdehid (MDA) ve protein oksidasyon ürünü protein karbonil miktarları (PCO) ile detoksifikasyon enzimi glutatyon-S-transferaz (GST) aktivitesi üzerine etkisi incelenmiştir. Borik asitin denenen konsantrasyonlarını içeren yapay besinler ile yetiştirilen D. melanogaster’ in üçüncü dönem larvalarının MDA miktarı önemli derecede artmıştır. Yüksek borik asit ile yetiştirilen erginlerin 10 gün süreyle 300 mg/L borik asit içeren besin ile beslenilmesi sonucunda kontrol besinine göre dişi ve erkek bireylerde MDA ve PCO miktarları önemli derecede artmıştır. Bu dişilerin yumurtalarındaki MDA, PCO miktarları ve GST aktivitesi önemli derecede artarken, PCO miktarındaki artış yaklaşık 31 katı oranında olmuştur. Sonuçlarımız, borik asitin yüksek konsantrasyonlarıyla beslenen D. melanogaster’in tüm gelişme dönemlerindeki oksidatif stres indikatörleri ve detoksifikasyon enzimi üzerine oldukça etkili olduğunu göstermiştir Daha fazlası Daha az

Hyršl, Pavel | Büyükgüzel, Ender | Büyükgüzel, Kemal

Article | 2008 | Acta Biologica Hungarica59 ( 3 ) , pp.281 - 288

The dietary effects of boric acid (BA) on the protein profiles of greater wax moth, Galleria mellonella (L.), were investigated in hemolymph and fat body of final instar (VIIth) and pupae. The insects were reared from first-instar larvae on an artificial diets containing 156, 620, 1250 or 2500 ppm of BA. We detected many undetermined protein fractions (6.5-260 kDa) in addition to well-defined protein fractions such as lipophorins and storage proteins in the tissues by using sodium dodecyl-sulphate polyacrylamide gradient gel electrophoresis. A marked quantitative change in the 45 kDa protein fraction of the hemolymph was observed in . . . the VIIth instar larvae reared on 2500 ppm dietary BA. © 2008 Akadémiai Kiadó Daha fazlası Daha az