Akyurek, S. | Ozdemir, B. S. | Sert, F. | Yalman, D. | Yavas, G. | Cengiz, M. | Bakkal, Bekir Hakan

Conference Object | 2016 | INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS96 ( 2 )

58th Annual Meeting of the American-Society-for-Radiation-Oncology (ASTRO) -- SEP 25-28, 2016 -- Boston, MA WOS: 000387655803461

Bektaş, Sibel | Karakaya, Kemal | Can, Murat | Bahadır, Buarak | Güven, Berrak | Erdoğan, Nilsen | Özdamar, Şükrü Oğuz

Article | 2016 | Kaohsiung Journal of Medical Sciences32 ( 7 ) , pp.339 - 347

The aim of this study was to investigate the effects of tadalafil (TDF) and pentoxifylline (PTX) on hepatic apoptosis and the expressions of endothelial and inducible nitric oxide synthases (eNOS and iNOS) after liver ischemia/reperfusion (IR). Forty Wistar albino rats were randomly divided into five groups (n = 8) as follows: sham group; IR group with ischemia/reperfusion alone; low-dose and high-dose TDF groups received 2.5 mg/kg and 10 mg/kg TDF, respectively; and PTX group received 40 mg/kg PTX. Blood was collected for the analysis of serum alanine aminotransferase, aspartate aminotransferase, ?-glutamyl transferase, uric acid, . . .malondialdehyde (MDA), and total antioxidant capacity (TAC). MDA and TAC also were measured in liver tissue. Histopathological examination was performed to assess the severity of hepatic injury. Apoptosis was evaluated using the apoptosis protease-activating factor 1 (APAF-1) antibody; the expressions of eNOS and iNOS were also assessed by immunohistochemistry in all groups. Serum alanine aminotransferase, aspartate aminotransferase, ?-glutamyl transferase, uric acid, MDA, and TAC, tissue MDA and TAC levels, hepatic injury, and score for extent and for intensity of eNOS, iNOS, and apoptosis protease-activating factor 1 were significantly different in TDF and PTX groups compared to the IR group. High dose-TDF and PTX have the best protective effect on IR-induced liver tissue damage. This study showed that TDF and PTX supplementation may be helpful in preventing free oxygen radical damage, lipid peroxidation, hepatocyte necrosis, and apoptosis in liver IR injury and minimizing liver damage. © 201 Daha fazlası Daha az

Özaçmak, Veysel Haktan | Özaçmak-Sayan, Hale | Barut, Figen

Article | 2016 | Nutritional Neuroscience19 ( 4 ) , pp.176 - 186

Objectives: Resveratrol appears to have neuroprotective potential in various animal models of brain disorders including cerebral ischemia and neurodegenerative diseases. Chronic cerebral hypoperfusion is a well-known pathological condition contributing to the neurodegenerative diseases such as vascular dementia. Purpose of the present study is to evaluate the possible therapeutic potential of resveratrol in a model of vascular dementia of ovariectomized female rats. Assessment of the potential was based on the determination of brain oxidative status, caspase-3 level, glial fibrillary acidic protein (GFAP), and neuronal damage on hip . . .pocampus and cerebral cortex. Methods: For creating the model of chronic cerebral hypoperfusion, ovariectomized female Wistar rats were subjected to the modified two vessel occlusion method, with the right common carotid artery being occluded first and the left one a week later. Results: At the 15th day following the ligation, neuronal damage was accompanied by the increased immunoreactivities of both GFAP and caspase-3, and significant neurodegeneration was evident in the hippocampus and cortex, all of which were significantly alleviated with resveratrol treatment (10 mg/kg). Biochemical analysis revealed that the resveratrol treatment decreased lipid peroxidation and restored reduced glutathione level as well. Discussion: The collected data of the present study suggest that the administration of resveratrol may provide a remarkable therapeutic benefit for vascular dementia, which is most likely related to the prevention of both apoptotic cell death and oxidative stress. We believe that therapeutic efficacy of resveratrol deserves to be tested for potential clinical application in postmenopausal elderly women suffering from vascular dementia. © 2015 W. S. Maney & Son Ltd 2015 Daha fazlası Daha az

Kuzu F. | Arpaci D. | Unal M. | Altas A. | Haytaoglu G. | Can M. | Barut F.

Article | 2016 | International Journal of Endocrinology2016 , pp.176 - 186

Background. Midkine (MK), a new heparin-binding growth factor, plays important roles in a variety of biological phenomena such as carcinogenesis, inflammation, and angiogenesis. In this study, we aimed to evaluate serum midkine (SMK) and nodular midkine (NMK) levels in patients with thyroid nodules to predict malignancy and whether there was any association between. Methods. A total of 105 patients (74 women, 31 men) with thyroid nodules were enrolled. The levels of SMK and NMK were measured. Any possible correlation between SMK, NMK, and biochemical, cytopathological, or radiological variables was investigated. Results. Both SMK an . . .d NMK were found to be higher in hypoechoic nodules with an irregular border and without a halo (p < 0.05). Serum MK levels were significantly higher in nodules with microcalcifications than nodules with macrocalcification or without calcification (p = 0.001). SMK levels were found to be correlated with NMK levels (SMK 0.63 ng/ml versus 1.04 ng/mL and NMK 0.55 ng/mL versus 0.55 ng/mL, r 2 = 0.54, p < 0.001). Conclusion. Both SMK and NMK can predict tumorigenesis of highly malignant/suspicious thyroid cytopathology and also well correlated with sonographic features of thyroid nodules. We suggest that MK levels may serve as an alternative biomarker, in conjunction with the cytopathological results in preoperative assessment of thyroid nodules. © 2016 Fatih Kuzu et al Daha fazlası Daha az

Demirel B.G. | Koca R. | Tekin N.S. | Kandemir N.O. | Gün B.D. | Köktürk F.

Article | 2016 | Turkderm Deri Hastaliklari ve Frengi Arsivi50 ( 4 ) , pp.136 - 140

Background and Design: Classic Kaposi's sarkoma (CKS) is a rare disease, generally seen across Mediterranean and the Middle East region. It's an angioproliferative disorder associated with human herpes virus-8 infection. There is a few data on epidemiology and clinical characteristics among Turkish patients with CKS. This study aims to evaluate epidemiologic, clinical characteristics and treatment results in patients with the diagnosis of CKS in Zonguldak. Materials and Methods: We retrospectively evaluated the hospital records of patients with CKS who attended the dermatological and venereal diseases department between 2003 and 201 . . .4. Seventy-four patients were included in this study. Demographic and clinical characteristics, applied treatments and responses to treatments were evaluated. Results: During the eleven year examination period, 74 CKS patients have been diagnosed in the dermatology clinic. The prevalence of CKS among dermatologic patients was found to be 0.02%. Patient age at diagnosis ranged from 33 to 90 years (mean: 70.2±11.7). Fifty-two patients were male (70.3%) and 22 patients were female (29.7%). Multiple nodules were the most frequently seen clinical forms and the distal lower extremity was the most common site of involvement (80.6%). According to the CKS staging system, it was observed that 47 patients (62.7%) were at stage 1, 11 patients (15.49%) at stage 2, eight patients (10.7%) at stage 3, and six patients (8%) were at stage 4. Treatment options were excision for 35.1% of patients (n=26), radiotherapy for 25.7% of patients (n=19), cryosurgery for 14.9% of patients (n=11), and chemotherapy for 10.8% of patients (n=8). Relapse was found to occur most commonly after excision (58.3%). Conclusion: Larger, multicenter studies are needed in order to determine the prevalence of CKS and characteristics of patients with CKS in our country. © Telif Hakk 2016 Deri ve Zührevi Hastaliklar Dernegi Daha fazlası Daha az

Barut F. | Kandemir N.O. | Gun B.D. | Ozdamar S.O.

Article | 2016 | Journal of the Pakistan Medical Association66 ( 7 ) , pp.905 - 907

T-cell/histiocyte-rich large B-cell lymphoma is an unusually encountered lymphoid neoplasm of stomach with aggressive course, and is an uncommon morphologic variant of diffuse large B-cell lymphoma. An ulceratedmass, 7x5x1 cm in size was observed within the gastrectomy specimen of a 76-year-old female patient. In cross sections, besides mature lymphoid cells displaying T-cell phenotype, a neoplastic formation composed of large, pleomorphic atypical lymphoid cells with, prominent nucleoli, vesicular nuclei and abundant eosinophilic cytoplasm displaying B-cell phenotype were observed. Meanwhile, histiocyte-like mononuclear cells and R . . .eed-Sternberg-like multinuclear cells expressing CD68 and Mac387 were also observed. The diagnosis of the case was T cell/histiocyte-rich large B-cell lymphoma. This rarely encountered neoplasm should be kept in mind in the differential diagnosis of primary gastric lymphomas. © 2016, Pakistan Medical Association. All rights reserved Daha fazlası Daha az

Barut F. | Udul P. | Kokturk F. | Kandemir N.O. | Keser S.H. | Ozdamar S.O.

Article | 2016 | Kaohsiung Journal of Medical Sciences32 ( 10 ) , pp.494 - 500

The evidence that PITX1 (pituitary homeobox 1) is a significant tumor suppressor in human cancer remains largely circumstantial, but it clearly warrants further study as little is known about the tumor-inhibitory roles of PITX1 in cutaneous malignant melanoma. The aims of this study were to investigate PITX1 gene expression in patients with cutaneous malignant melanoma and to evaluate its potential relevance to clinicopathological characteristics and tumor cell proliferation. Clinicopathological findings of patients with cutaneous malignant melanoma were analyzed retrospectively. PITX1 and Ki-67 expression were detected by immunohis . . .tochemistry in malignant melanoma and healthy tissue samples from each patient. Labeling indices were calculated based on PITX1 gene and Ki-67 expression. The correlation between PITX1and Ki-67 expressions was analyzed in cutaneous malignant melanoma cases. The relationship between PITX1 expression intensity and clinicopathological characteristics was also analyzed. PITX1 expression was observed in all (100%) normal healthy skin tissue samples. In addition, PITX1 expression was found in 56 (80%) and was absent in 14 (20%) of the 70 cutaneous malignant melanoma cases. Ki-67 positive expression was only detected in the 14 (20%) PITX1-negative cases. PITX1-positive tumor cells were observed on the surface, but Ki-67 positive tumor cells were observed in deeper zones of the tumor nests. PITX1 expression was downregulated in human cutaneous malignant melanoma lesions compared with healthy skin tissue, but Ki-67 expression was upregulated in concordance with the progression of cutaneous malignant melanoma. PITX1 expression may be involved in tumor progression and is a potential tumor suppressor gene and prognostic marker for cutaneous malignant melanoma. © 201 Daha fazlası Daha az

Demirel B.G. | Tekin N.S. | Barut F. | Aslaner M. | Koca R. | Gencer D.

Article | 2016 | Erciyes Tip Dergisi38 ( 2 ) , pp.78 - 80

Langerhans cell histiocytosis (LCH) is a rare group of idiopathic diseases characterized by abnormal proliferation of bone marrow-derived dendritic cells called histiocytes. It is more common in childhood, but in the literature, some adult cases have been reported. For several reasons, the disease is more familiar to pediatricians than it is to physicians handling adult cases; therefore, the diagnosis of adult cases is often delayed or missed. The clinical course of LCH is variable, ranging from a self-healing solitary bone lesion to a widely disseminated life-threatening disease. The diagnosis of LCH should be based on histologic a . . .nd immunophenotypic examination of perilesional skin. In this report, we present a case of LCH in a 66-yearold woman with bone, skin, and pituitary gland involvement; this case emphasizes that a patient with LCH should undergo careful multidisciplinary evaluation. © 2016 by Erciyes University School of Medicine Daha fazlası Daha az

Demirel, Beril Gülüş | Tekin, Nilgün Solak | Barut, Figen | Aslaner, Müzeyyen | Koca, Rafet | Gencer, Duygu

Article | 2016 | Erciyes Medical Journal38 ( 2 ) , pp.78 - 80

Langerhans cell histiocytosis (LCH) is a rare group of idiopathic diseases characterized by abnormal proliferation of bone marrow-derived dendritic cells called histiocytes. It is more common in childhood, but in the literature, some adult cases have been reported. For several reasons, the disease is more familiar to pediatricians than it is to physicians handling adult cases; therefore, the diagnosis of adult cases is often delayed or missed. The clinical course of LCH is variable, ranging from a self-healing solitary bone lesion to a widely disseminated life-threatening disease. The diagnosis of LCH should be based on histologic a . . .nd immunophenotypic examination of perilesional skin. In this report, we present a case of LCH in a 66-yearold woman with bone, skin, and pituitary gland involvement; this case emphasizes that a patient with LCH should undergo careful multidisciplinary evaluation. Langerhans cell histiocytosis (LCH) is a rare group of idiopathic diseases characterized by abnormal proliferation of bone marrow-derived dendritic cells called histiocytes. It is more common in childhood, but in the literature, some adult cases have been reported. For several reasons, the disease is more familiar to pediatricians than it is to physicians handling adult cases; therefore, the diagnosis of adult cases is often delayed or missed. The clinical course of LCH is variable, ranging from a self-healing solitary bone lesion to a widely disseminated life-threatening disease. The diagnosis of LCH should be based on histologic and immunophenotypic examination of perilesional skin. In this report, we present a case of LCH in a 66-yearold woman with bone, skin, and pituitary gland involvement; this case emphasizes that a patient with LCH should undergo careful multidisciplinary evaluation Daha fazlası Daha az

Barut, Figen | Özaçmak, Veysel Haktan | Turan, İnci | Özaçmak-Sayan, Hale | Aktunç, Erol

Article | 2016 | Clinical and Investigative Medicine39 ( 1 ) , pp.78 - 80

Purpose: Multiple organ failure, including acute lung injury, is a common complication of intestinal ischemia and reperfusion (I/R) injury and contributes to its high mortality rate. Activated polymorphonuclear neutrophils and reactive oxygen species contribute to the lung injury caused by intestinal I/R. Mineralokortikoid receptor antagonist spironolactone has a protective effect against I/R injury in animal models of retina, kidney, heart, and brain. The aim of the present study is to investigate the effect of aldosteron receptor blocker spironolactone on lung injury induced by intestinal I/R. Methods: Wistar albino rats were divi . . .ded into four groups: (1) sham control; (2) intestinal I/R (30 min of ischemia by superior mesenteric artery occlusion followed by 3 h of reperfusion); (3) spironolactone pretreatment (20 mg/kg) + I/R; and, (4) spironolactone pretreatment without I/R. Spironolactone was given orally 3 days prior to intestinal I/R. A marker for lipid peroxidation (malondialdehyde; MDA), an indicator or oxidation state (reduced glutathione; GSH), an index of polymorphonuclear neutrophil sequestration (myeloperoxidase; MPO), inducible nitric oxide synthase (iNOS) immunoreactivity, and the histopathology of the lung tissue were analyzed. Results: Spironolactone pretreatment markedly reduced intestinal I/R-induced lung injury as indicated by histology and MDA and MPO levels. Moreover, the pretreatment decreased the iNOS immunoreactivity. Conclusion: The present study strongly suggests that spironolactone pretreatment decreased neutrophil infiltration, iNOS induction, oxidative stress, and histopathological injury in an experimental model of intestinal I/R induced-lung injury of rats. © 2016 CIM Daha fazlası Daha az