Büyükgüzel, Ender | Kayaoğlu, Selver

Makale | 2014 | Türkiye Entomoloji Dergisi38 ( 1 ) , pp.83 - 99

Salisilanilidler bağırsak şeridi gibi parazitlerin mitokondrisinde oksidatif fosforilasyonu inhibe ederek etkisini gösterirler ve bu antiparazitik ilaçlar tıpta ve veterinerlikte kullanılmaktadır. Niklozamidin potansiyel antihelmintik aktivitesi memelilerde iyi bir şekilde ortaya konulmuş, bu çalışmada da Galleria mellonella larvaları kullanılarak niklozamidin in vivo insektisit etkisi araştırılmıştır. Niklozamid, 7. dönem larva, pupa ve ergin evrelerinin yaşama oranını önemli derecede düşürürken, en yüksek konsantrasyonu (% 1,0) ergin gelişme süresini önemli derecede uzatmıştır. Dişilerin yumurta verimi kontrol besininde 78,6 ± 6,1 . . . yumurta sayısı/gün/dişi olarak tespit edilmiştir. % 0,1’lik niklozamid ile beslenildiğinde ise bu değer 114,7 ± 10,9 yumurta sayısı/gün/dişi’ a yükselmiştir; bunun yanısıra en yüksek konsantrasyonda (% 1,0) hiç yumurta elde edilememiştir. Niklozamidin en yüksek konsantrasyonunda (% 1,0) erkek ergin ömür uzunluğu artmıştır. Ayrıca, niklozamid yumurta açılımını da denenen tüm konsantrasyonlarda önemli oranda düşürmüştür. % 0,1’lik niklozamid konsantrasyonunda malondialdehit (MDA) miktarı 4 kat, glutatyon- S-transferaz enzimi (GST) aktivitesi ise 2 kat artmıştır. Kontrol besinine göre (133,24 ± 23,6 nmol/mg protein) niklozamidin denenen konsantrasyonları protein karbonil (PCO) miktarını en az 5 kat (701,24 - 808,02 nmol/mg protein) önemli derecede arttırmıştır. Bu çalışmada Galleria mellonella model böcek olarak kullanılarak, böceklerle mücadelede belirli klinik öneme sahip antihelmintik ilaçların aktif şekilde kullanılabilirliği belirtilmiştir. Ayrıca, bu çalışmanın sonuçları niklozamidin prooksidan etkisine bağlı olarak biyolojik ve aynı zamanda böceğin antioksidan savunma cevabı üzerine negatif etkisi olduğunu göstermiştir. The salisilanilides function by inhibiting mitocondrial oxidative phosphorylation in parasitic tapeworms and thus they are used as an antiparasitic drug in medicine and veterinary. While the potent antihelmintic activity of niclosamide has been well characterised in mammals, this study investigated the in vivo insecticide effect of niclosamide using larvae of the insect Galleria mellonella. Niclosamide was successful in decreasing the survival of 7th instar larvae, pupal and adult stages while only the highest concentration of this antihelmintic antibiotic (1.0 %) significantly prolonged developmental time to adult stage. Fecundity of females was obtained as 78.6 ± 6.1 number of eggs/day/female in control diet. Fecundity were increased to 114.7 ± 10.9 at 0.1% of niclosamide. However, we could not obtain any egg at the highest concentration. An increase in the male adult longevity was obtained when reared with the highest concentrations of niclosamide. Niclosamid rearing resulted in a decrease in hatchability of eggs. Niclosamide at 0.1 % of concentration increased malondialdehyde (MDA) content (4-fold), glutathion-S-transferase (GST) activity (2-fold). Relative to control (133.24 ± 23.6 nmol/mg protein), niclosamide at tested concentrations significantly increased protein carbonyl (PCO) content at least 5-fold (701.24- 808.02 nmol/mg protein). This work indicates that G. mellonella larvae may be used as a good model to ascertain importance of clincally important antihelmintic drug active ingredients in chemical management of pest insects. The results of this work also indicate that the negative effects of niclosamide on insect biology are due to its pro-oxidant properties and also to the ability of niclosamide in crippling the insect’s antioxidan defence response Daha fazlası Daha az

Büyükgüzel, Ender

Makale | 2014 | Journal of Entomological Science49 ( 2 ) , pp.144 - 155

Eicosandoids, or icosanoids, are signaling compounds created by the oxidation of 20-carbon fatty acids. They control many complex physiological and immunological functions in vertebrate and invertebrate animals. This study tested the hypothesis that eicosanoids act in insect antioxidant defense. The effects of 3 eicosanoid biosynthesis inhibitors (EBIs) - dexamethasone, esculetin, and phenidone - on the oxidative stress indicator, malondialdehyde (MDA), and the detoxification enzyme, glutathione S-transferase (GST), was examined in the midgut of larvae of the greater wax moth, Galleria mellonella (L). The larvae were reared on artif . . .icial diets supplemented with 0.001, 0.01, 0.1 or 1.0% of the EBIs. Esculetin, which is a lipoxygenase inhibitor, significantly increased MDA content; whereas, GST activity was significantly increased at only the highest concentration tested. Dexamethasone, a phospholipase A2 inhibitor, significantly increased MDA content and GST activity at concentrations of 0.01, 0.1, and 1.0%. Phenidone, a dual inhibitor of cyclooxygenase and lipoxygenase, increased MDA content, whereas the 0.01 and 0.1% concentrations of phenidone significantly increased GST activity. Our results indicate that antioxidative responses are, at least in part, controlled by a physiological system that includes eicosanoid biosynthesis Daha fazlası Daha az

İçen, Ender | Armutçu, Ferah | Büyükgüzel, Kemal | Gürel, Ahmet

Makale | 2005 | Journal of Economic Entomology98 ( 2 ) , pp.358 - 366

Although acetylcholinesterase (AChE) is the primary target of organophosphorus insecticides (OPs), increasing evidence regarding their secondary effects suggests that OPs disturb homeostasis of insects by generating free radical intermediates that trigger lipid peroxidation. We therefore investigated alterations in lipid peroxidation product, malondialdehyde (MDA) content, and alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities, in conjunction with AChE activity as biochemical stress indicators in greater wax moth, Galleria mellonella (L.) larvae for OPs methyl parathion (MP) and ethyl parathion (EP). The . . .effects of MP and EP were first investigated by rearing the young larvae on an artificial diet containing 0.01, 0.1, 1, 10, and 100 ppm of each insecticide. Second, the mature larvae were injected with 0.05, 0.5, 5, 50, and 500 ng of insecticides for determining the changes in biochemical stress responses. The diet with lowest level of MP significantly decreased the activities of all measured enzymes, whereas it increased MDA content. However ALT and AST were significantly higher in the larvae reared with the diet with high levels of MP than in control larvae. All tested levels of MP resulted in a decrease in AChE activity. The lowest level of EP in diet (0.01 ppm) significantly increased ALT activity, whereas it reduced that of AChE. This insecticide at 0.1 ppm resulted in reduced AST activity, but 1 ppm in diet elevated AST activity and MDA content. EP at 0.1 ppm and higher levels in the diet reduced ALT activity. All dietary EP levels significantly decreased AChE activity. ALT, AST, and AChE were lower in larvae fed with the diet containing 100 ppm ethyl parathion compared with larvae on control diet. MP at 50 ng per larva increased ALT and AST activities from 35.42 ± 0.74 and 26.34 ± 0.83 to 203.57 ± 1.09, and 122.90 ± 1.21 U/g, respectively, when the mature larvae were injected. All injected doses of EP dramatically reduced both ALT and AST activities, but only the lowest and highest levels of this insecticide decreased AChE activity. The lowest level of this insecticide also significantly increased MDA content in larvae. High levels of both insecticides increased MDA content. We observed a significant higher increase in MDA content in the larvae reared with 10 ppm EP (102.16 ± 1.57 nmol/g protein) than the control group (30.28 ± 1.42 nmol/g protein). These results suggest that OPs caused the metabolic and synaptic dysfunctions in greater wax moth and alter its biochemical physiology in response to oxidative stress. © 2005 Entomological Society of America Daha fazlası Daha az

Büyükgüzel, Ender

Makale | 2012 | Archives of Insect Biochemistry and Physiology81 ( 1 ) , pp.20 - 33

Nodulation is the predominant insect cellular immune response to microbial infections. We posed the hypothesis that parasitoid insects in their adulthood express melanotic nodulation reactions to viral challenge and that eicosanoids mediate nodulation reactions and phenoloxidase (PO) activation in response to viral challenge. To test this idea, we injected Pimpla turionellae adults with indomethacin, a nonsteroidal anti-inflammatory drug, immediately prior to intrahemocoelic injection of Bovine herpes simplex virus-1 (BHSV-1). Treating newly emerged adults of P. turionellae with BHSV-1 induced nodulation reactions, and decreased PO . . .activity at high viral doses. Relative to vehicle-treated controls, indomethacin-treated adults produced significantly reduced numbers of nodules following viral infection (down from approximately 21 nodules per adult to less than six nodules per adult). In addition to injection treatments, increasing dietary indomethacin dosages (from 0.01% to 0.1%) were associated with decreasing nodulation (by six-fold) and PO (by about three-fold) reactions to BHSV-1 injection. Wasp adults orally fed with the lowest dietary indomethacin concentration (0.001%) expressed significantly increased PO activity (1.45 unit/min/mg protein) while nodulation reaction was not affected in response to viral challenge compared to control adults. We infer from these findings that cyclooxygenase (COX) products, at least prostaglandins, mediate nodulation response and PO action to viral infection in adults of these highly specialized insects. © 2012 Wiley Periodicals, Inc Daha fazlası Daha az

Büyükgüzel, Ender | Hyršl, Pavel | Büyükgüzel, Kemal

Makale | 2010 | Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology156 ( 2 ) , pp.176 - 183

Antioxidant enzymes play a major role in the defense against pro-oxidative effects of xenobiotics and pro-oxidant plant allelochemicals in insects. We posed the hypothesis that eicosanoids also mediate antioxidant enzymatic defense reactions to pro-oxidant challenge. To test this idea, we reared first-instar larvae of Galleria mellonella (L.) with the lypoxygenase inhibitor, esculetin (0.001%), the phospholipase A2 inhibitor, dexamethasone (0.001%) and the dual inhibitor of cyclooxygenase and lipoxygenase, phenidone (0.1%) to seventh-instars. Newly ecdysed seventh-instars were then fed on artificial diet containing 0.05% xanthotoxin . . . (XA) for 2 days. Treating seventh-instar larvae of G. mellonella with XA induced lipid peroxidation and protein carbonylation as evident from the increased content of malondialdehyde (MDA) and protein carbonyls respectively, and antioxidative enzymatic response in a dose-dependent manner. High dietary XA concentrations (0.005 and 0.1%) were associated with increasing MDA and carbonyl content (by 3-fold) and antioxidant enzyme activities, superoxide dismutase (SOD) (by 3-fold) and catalase (CAT) (by 4-fold), and glutathione-dependent enzymes, glutathione S-transferase (GST) (by 15-fold) and glutathione peroxidase (GPx) (by 7-fold). Relative to control, eicosanoid biosynthesis inhibitors (EBIs) esculetin, dexamethasone and phenidone also resulted in impaired MDA content and antioxidant enzyme activities. However, carbonyl content did not differ between control- and EBIs-feeding larvae. Finally, MDA and carbonyl content, and antioxidant enzymes SOD, GST and GPx activities exhibited an incremental increase while CAT activity was decreased in the experimental larvae that had been reared on media amended with esculetin, dexamethasone and phenidone and then challenged with our standard XA challenge dose. Two of the markers indicated that significantly higher levels of oxidative stress were produced in the hemolymph tissue of larvae fed diets supplemented with EBIs and then challenged with XA. This oxidative stress was associated with elicited antioxidative responses by increasing SOD, GST and GPx and decreasing CAT activities in hemolymph. We infer from these findings that eicosanoids mediate insect antioxidant enzymatic responses to dietary pro-oxidants. © 2010 Elsevier Inc. All rights reserved Daha fazlası Daha az

Chowanski, Szymon | Adamski, Zbigniew | Marciniak, Pawel | Rosiński, Grzegorz | Büyükgüzel, Ender | Büyükgüzel, Kemal | Falabella, Patrizia | Scrano, Laura | Ventrella, Emanuela | Lelario, Filomena | Bufo, Sabino A.

Makale | 2016 | Toxins8 ( 3 ) , pp.176 - 183

Only a small percentage of insect species are pests. However, pest species cause significant losses in agricultural and forest crops, and many are vectors of diseases. Currently, many scientists are focused on developing new tools to control insect populations, including secondary plant metabolites, e.g., alkaloids, glycoalkaloids, terpenoids, organic acids and alcohols, which show promise for use in plant protection. These compounds can affect insects at all levels of biological organization, but their action generally disturbs cellular and physiological processes, e.g., by altering redox balance, hormonal regulation, neuronal sign . . .alization or reproduction in exposed individuals. Secondary plant metabolites cause toxic effects that can be observed at both lethal and sublethal levels, but the most important effect is repellence. Plants from the Solanaceae family, which contains numerous economically and ecologically important species, produce various substances that affect insects belonging to most orders, particularly herbivorous insects and other pests. Many compounds possess insecticidal properties, but they are also classified as molluscides, acaricides, nematocides, fungicides and bactericides. In this paper, we present data on the sublethal and lethal toxicity caused by pure metabolites and crude extracts obtained from Solanaceae plants. Pure substances as well as water and/or alcohol extracts cause lethal and sublethal effects in insects, which is important from the economical point of view. We discuss the results of our study and their relevance to plant protection and management Daha fazlası Daha az

Güneş, Eda | Büyükgüzel, Ender

Makale | 2017 | Türkiye Entomoloji Dergisi41 ( 1 ) , pp.3 - 15

Tarımsal zararlılar ile mücadelede sentetik organik insektisitler yoğun olarak kullanılmaktadır. Borik asit organik kimyasal insektisitlere göre hedef olmayan organizmalara karşı daha düşük toksisiteye sahip olması nedeniyle önem taşımaktadır. Bu amaçla çalışmamızda meyve sineği Drosophila melanogaster Meigen,1830 (Diptera: Drosophilidae)'in birinci dönem larvaları borik asitin farklı konsantrasyonlarını (10, 100, 200 ve 300 mg/L) içeren yapay besinler ile yetiştirilmiştir. Drosophila melanogaster' in üçüncü dönem larva, pupa, ergin dönemleri ve yumurtalarında oksidatif stresin önemli indikatörleri olan lipid peroksidasyonu ürünü ma . . .londialdehid (MDA) ve protein oksidasyon ürünü protein karbonil miktarları (PCO) ile detoksifikasyon enzimi glutatyon-S-transferaz (GST) aktivitesi üzerine etkisi incelenmiştir. Borik asitin denenen konsantrasyonlarını içeren yapay besinler ile yetiştirilen D. melanogaster' in üçüncü dönem larvalarının MDA miktarı önemli derecede artmıştır. Yüksek borik asit ile yetiştirilen erginlerin 10 gün süreyle 300 mg/L borik asit içeren besin ile beslenilmesi sonucunda kontrol besinine göre dişi ve erkek bireylerde MDA ve PCO miktarları önemli derecede artmıştır. Bu dişilerin yumurtalarındaki MDA, PCO miktarları ve GST aktivitesi önemli derecede artarken, PCO miktarındaki artış yaklaşık 31 katı oranında olmuştur. Sonuçlarımız, borik asitin yüksek konsantrasyonlarıyla beslenen D. melanogaster'in tüm gelişme dönemlerindeki oksidatif stres indikatörleri ve detoksifikasyon enzimi üzerine oldukça etkili olduğunu göstermiştir Synthetic organic insecticides are widely used to combat agricultural pests. Boric acid has a great importance in pest management because it has less toxic effect on non-target organisms compared to other organic chemical insecticides. For this purpose, the fruit fly Drosophila melanogaster Meigen, 1830 (Diptera: Drosophilidae) was reared from first stage larvae on an artificial diet containing boric acid at 10, 100, 200 or 300 mg/L to adult stage. The effect of boric acid on important oxidative stress indicators such as lipid peroxidation product of malondialdehyde contents (MDA) and protein oxidation products of protein carbonyl contents (PCO) and detoxification enzyme activity of glutathione S-transferase (GST) in the third stage larvae, pupae, adults and eggs of D. melanogaster were investigated. All boric acid concentrations significantly increased MDA content in third stage larva. When the adults from the larvae reared on 300 mg/L of dietary BA were also fed with high BA concentration for a 10-day period, MDA and PCO contents of male and female adults were considerably went up in comparison to control. MDA and PCO content in the eggs of these females were hugely increased. The rise in PCO content of the eggs was 31-fold relative to control. Our results indicate that BA feeding at high concentrations in all developmental stages of D. melanogaster is more effective on oxidative stress indicators and detoxification enzym Daha fazlası Daha az

Kılıç, Ali | Büyükgüzel, Kemal | Büyükgüzel, Ender

Makale | 2015 | KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI21 ( 6 ) , pp.841 - 847

The effect of triclabendazole, which is a benzimidazole anthelmintic, on the survival rate and developmental time in different stages of greater wax moth Galleria mellonella (L.) was investigated by rearing the larvae on the artificial diets. Triclabendazole was incorporated into diets at concentrations of 0.001, 0.01 or 0.1%. G. mellonella larvae was reared from first instar larvae to adult emergence on the artificial diets with different concentrations of triclabendazole. The survival rate in seventh instar (7th-instar) and adult stage were significantly lower at low concentrations of this anthelmintic agent (0.001 and 0.01 g per . . .100 g of diet) than the control, while there were no differences on pupation in comparison to control diet. However, the pupation and adult emergence were significantly lower at the highest dietary concentration of triclabendazole than control group but there were no differences on survival of 7th-instar larvae. The highest concentration of the anthelmintic agent (0.1 g/100 g diet) decreased pupation from 81.6 +/- 4.32% to 43.3 +/- 7.45%, adult emergence rate from 73.3 +/- 2.36 to 13.3 +/- 2.36. Triclabendazole at the highest concentration prolonged developmental time to 7th larval stage by 3.8 days reaching total 22.8 +/- 1.54 days. This diet containing 0.1% of triclabendazole significantly prolonged pupal developmental time from 24.6 +/- 1.24 days to 28.4 +/- 1.24 days, adult developmental time from 35.3 +/- 1.27 days to 42.0 +/- 1.62 days. This study is of importance in appreciation for usage of antihelmintic with different structure and mode of actions in the management of pest insects to reduce damage to environment and nontarget organisms Daha fazlası Daha az

Büyükgüzel, Ender | Büyükgüzel, Kemal

Makale | 2019 | Journal of Entomological Science54 ( 1 ) , pp.79 - 86

Galleria mellonella L. larvae were reared on a standard artificial diet amended with various concentrations (0.5%, 1.0%, 1.5%, and 2.0%) of the aminocoumarin DNA gyrase inhibitor antibiotic drug novobiocin. The effects of the inhibitor on survival and development of the larvae were measured. Survival rates for 7th instars, pupae, and emerging adults were 90.0%, 86.2%, and 76.2%, respectively, in the control diet. These rates were decreased to 15.0%, 7.5%, and 6.2% for larvae fed the diet with the highest novobiocin concentration (2.0%). High concentrations of the antibiotic caused prolongation in the larval, pupal, and adult develop . . .mental periods. Our results indicate that this gyrase inhibitor can be used as dietary additive in artificial rearing of G. mellonella when its concentrations are ascertained. © 2019 Georgia Entomological Society Inc. All rights reserved Daha fazlası Daha az

Hız, Pınar | Erdem, Meltem | Büyükgüzel, Ender | Büyükgüzel, Kemal

Makale | 2016 | Kafkas Üniversitesi Veteriner Fakültesi Dergisi22 ( 5 ) , pp.777 - 784

Büyük bal mumu güvesi Galleria mellonella L. mikrobiyal enfeksiyonların ve klinik ilaçların çalışılmasında memeli modellerine alternatif bir model olarak bilindiği gibi insektisit etkinliği çalışmalarında da model böcek olarak yaygın kullanıma sahiptir. Bu böceğin larvaları yapay besin ortamında beslenerek fluorokinolon sınıfından bir antibiyotik olan gemifloksasinin böceğin eşey oranı, dişi ve erkek ömür uzunluğu, yumurta verimi, açılma oranı gibi ergin biyolojik özellikleri üzerine etkisi laboratuvar şartlarında incelendi. Böceğin birinci evre larvaları %0.001, 0.01, 0.1 ve 1.0 oranında gemifloksasin içeren yapay besinler ile ergi . . .n evreye kadar beslendi. Gemifloksasinin denenen konsantrasyonlarını içeren besinler erkek ve dişi eşey oranı ile erginlerin ömür uzunluğu üzerinde etkili olmadığı halde yumurta verimini önemli derecede düşürdüğü belirlendi. Artan besinsel gemifloksasin konsantrasyonları ile ters orantılı olarak yumurta verimi ve açılma oranı azaldı. Kontrol besini ile yetiştirilen dişiler günde 134.46 adet yumurta üretirken gemifloksasinin yüksek miktarlarında dişiler 26.75 ve 53.5 yumurta bıraktılar. Yumurtaların açılma oranı da gemifloksasinin %0.01'lik konsantrasyonundan itibaren önemli derecede azalmış olup, en yüksek konsantrasyon bu oranı %53.71'e düşürdü. Bu çalışma erginlerin biyolojik özelliklerinin gemifloksasin tarafından önemli derecede etkilendiğini ve bu etkilerin antibiyotiğin konsantrasyonlarına bağımlı değişimler olduğunu gösterdi. Greater wax moth, Galleria mellonella L. is most commonly used as model insects for studying insecticidal efficiency of chemicals as well as it has been known as an alternative model to mamalian model for studying microbial infections and clinical drugs. The effects of a fluoroquinolone antibacterial agent, gemifloxacin, on sex ratio, male and female adult longevity, fecundity and hatchability of this insect were investigated by rearing the first instar larvae on artificial diets in the laboratory condition. The insect was reared from first-instar larvae to adult stage on an artificial diets containing gemifloxacin at 0.001, 0.01, 0.1 or 1.0%. Gemifloxacin did not affect male and female sex ratio and adult longevity while it significantly decreased egg number. Fecundity and hatchability were significantly and inversely decreased by increasing gemifloxacin concentrations. The females reared from control diet produced 134.46 per day, whereas high concentrations of gemifloxacin decreased the egg number to 26.75 and 53.5. Hatchability was also significantly decreased by 0.01% and above concentrations of gemifloxacin whereas, the highest concentration of antibiotic lowered the hatchability to 53.71%. This study indicated sublethal effects of gemifloxacin are likely to have a significant impact on adult biological traits and these effects show concentration-dependent variation in biological traits of the insect Daha fazlası Daha az

Erdem, Meltem | Büyükgüzel, Ender | Büyükgüzel, Kemal

Makale | 2016 | Journal of Entomological Science51 ( 4 ) , pp.305 - 313

he fruit fly Drosophila melanogaster (Meigen) (Diptera: Drosophilidae) is often used in various biological, molecular, and toxicological studies. Sodium tetraborate, a boron compound, was added to the artificial diet of developing D. melanogaster to determine its effects on adult longevity and fecundity. Insects reared from first instars to adults on diets containing 10, 30, 150, 300, or 400 mg/liter sodium tetraborate showed that the highest concentration (400 mg/liter) significantly reduced female longevity (31.65 6 4.02 [mean 6 SD] d for the controls versus 1.87 6 0.30 d for 400 mg/liter) and male longevity (32.80 6 1.96 d for co . . .ntrols versus 3.57 6 0.42 d for 400 mg/liter). Females from the control diet produced 9.46 6 0.57 (mean 6 SD) eggs per female, whereas those fed on a diet containing 300 mg/liter produced only 1.92 6 0.30 eggs per females. These results expand our knowledge of the impact of sodium tetraborate on various insects and indicate that boron compounds should be further investigated to ascertain their potential as an alternative control tactic for pest insects Daha fazlası Daha az

Çelik, Cihat | Büyükgüzel, Kemal | Büyükgüzel, Ender

Makale | 2019 | Journal of the Entomological Research Society21 ( 1 ) , pp.95 - 108

We investigated that the effects of oxyclozanide on survival rate, development time, adult longevity and the amount of total protein (TP) in different developmental stages (7th-instars larvae, pupae and adult) of greater wax moth Galleria mellonella L. First-instar larvae of insects were reared on 0.003, 0.03 and 0.3 g oxyclozanide in 100 g artificial diets. When compared to all tested concentrations of oxyclozanide and control diet without oxyclozanide, it was confirmed that this anthelmintic drug significantly reduced survival rate of 7th-instar larval stage, pupal and adult stage of G. mellonella. While 7th-instars larval rate is . . . 91.25 ± 6.21 % in the control diet without oxyclozanide, this rate has been determined 28.75 ± 3.24 % in the 0.3 % concentration of oxyclozanide. TP of the insect increased in response to all concentration of oxyclozanide in comparison to control diet. This considerable increase was expressed almost two-fold especially at the 0.003 and 0.03 % of oxyclozanide concentrations in all developmental stages. The results obtained from this research support that the oxyclozanide has effects on the survival, development, adult longevity and TP of the insect. © 2019 Gazi Entomological Research Society. All rights reserved Daha fazlası Daha az