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The effect of neomycin on survival and development of Pimpla turionellae L. (Hymenoptera: Ichneumonidae) reared on a natural host

Harmancı, Cumhur | Büyükgüzel, Kemal | Büyükgüzel, Ender

Article | 2019 | Journal of Economic Entomology112 ( 3 ) , pp.1081 - 1088

Understanding the effects of diet on metabolic events is crucial for biological control programs of parasitoid insects. As bioindicators of long-term physiological stress: survivorship of fifth instar larvae, pupation, adult survival, and developmental time for stages of endoparasitoid Pimpla turionellae L. (Hymenoptera: Ichneumonidae) were investigated by rearing the parasitoid on the host, Galleria mellonella L. (Lepidoptera: Pyralidae) pupae were treated with neomycin. First instar larvae of G. mellonella were reared on artificial diets containing 0.005, 0.01, or 0.5 g neomycin (g/100 g of diet) until seventh instar larvae; the p . . .upae from these larvae were used as a host for rearing P. turionellae. In the control group, the pupae from larvae reared on artificial diets without neomycin were used as a host. Survivorship of fifth instar, pupal, and adult stages of P. turionellae L. reared on G. mellonella pupae as a host fed with different concentrations of neomycin were significantly decreased in comparison to the control group. Approximately eighty percent of P. turionellae L. pupae were produced from control host pupae, while other neomycin concentrations significantly decreased the pupation of the parasitoid. Pimpla turionellae L. larvae reared on control host pupae reached fifth instar in about 9.6 ± 0.61 d, while the larvae reared from a host pupae exposed to the highest antibiotic concentration completed their development to the fifth instar in about 7.4 d. These results showed that neomycin, and possibly its metabolites, contaminated P. turionellae L. larvae from a host and affected larval stages of the parasitoid. © The Author(s) 2019. Published by Oxford University Press on behalf of Entomological Society of America Daha fazlası Daha az

2019-12-23

Arslan, Hülya | Yeşilyurt, Nazlı | Hazer, Baki

Proceedings | 2008 | MACROMOLECULAR SYMPOSIA269 , pp.23 - 33

4th European Symposium on Biopolymers -- OCT 02-04, 2007 -- Kusadasi, TURKEY WOS: 000258909100006

Karyotype of Taphozous nudiventris cretzschmar, 1830 (Mammalia: Chiroptera) from Turkey

Karataş, Ahmet | Sözen, Mustafa

Article | 2002 | Israel Journal of Zoology48 ( 4 ) , pp.359 - 360

[No abstract available]

In vivo application of poly-3-hydroxyoctanoate as peripheral nerve graft

Hazer, Derya Burcu | Bal, Ercan | Nurlu, Gülay | Benli, Kemal | Balcı, Serdar | Öztürk, Feral | Hazer, Baki

Article | 2013 | Journal of Zhejiang University: Science B14 ( 11 ) , pp.993 - 1003

Objective: This study aims to investigate the degree of biocompatibility and neuroregeneration of a polymer tube, poly-3-hydroxyoctanoate (PHO) in nerve gap repair. Methods: Forty Wistar Albino male rats were randomized into two groups: autologous nerve gap repair group and PHO tube repair group. In each group, a 10-mm right sciatic nerve defect was created and reconstructed accordingly. Neuroregeneration was studied by sciatic function index (SFI), electromyography, and immunohistochemical studies on Days 7, 21, 45 and 60 of implantation. Biocompatibility was analyzed by the capsule formation around the conduit. Biodegradation was . . .analyzed by the molecular weight loss in vivo. Results: Electrophysiological and histomorphometric assessments demonstrated neuroregeneration in both groups over time. In the experimental group, a straight alignment of the Schwann cells parallel to the axons was detected. However, autologous nerve graft seems to have a superior neuroregeneration compared to PHO grafts. Minor biodegradation was observed in PHO conduit at the end of 60 d. Conclusions: Although neuroregeneration is detected in PHO grafts with minor degradation in 60 d, autologous nerve graft is found to be superior in axonal regeneration compared to PHO nerve tube grafts. PHO conduits were found to create minor inflammatory reaction in vivo, resulting in good soft tissue response. © 2013 Zhejiang University and Springer-Verlag Berlin Heidelberg Daha fazlası Daha az

Exposure to streptomycin alters oxidative and antioxidative response in larval midgut tissues of Galleria mellonella

Büyükgüzel, Ender | Kalender, Yusuf

Article | 2009 | Pesticide Biochemistry and Physiology94 ( 02.Mar ) , pp.112 - 118

Although antibiotics have different molecular modes of actions, increasing evidence for their secondary effects suggests that they disturb cellular homeostasis by generating free radical intermediates that trigger lipid peroxidation, which leads to oxidative stress. Streptomycin is an antibiotic insecticide used to control pest insects and microbial diseases of agricultural crops. We investigated the biochemical basis for pro-oxidative effects of streptomycin in the midgut tissues of greater wax moth, Galleria mellonella (L.) seventh-instar larvae by measuring content of the oxidative stress indicator, malondialdehyde (MDA), and ant . . .ioxidant enzymes [superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST) and glutathione peroxidase (GPx)] and transaminases [alanine aminotransferase (ALT), aspartate aminotransferase (AST)] activities. The insects were reared from first-instar larvae on artificial diets containing 0.001, 0.01, 0.1 or 1.0 g streptomycin per 100 g of diets. The supplementation of streptomycin at high concentrations to the diets caused oxidative stress as evidenced by the elevation of MDA content, SOD and GPx activities, accompanied by the concurrent depletion of CAT and GST activities. The streptomycin-induced oxidative stress was also accompanied by decreases of transaminases activities in midgut tissues. We found a significant negative correlation of MDA contents with GST activities in the larval midgut tissues. These results suggest that exposure to dietary streptomycin resulted in oxidative stress which could impact midgut digestive physiology at the expense of impairment of antioxidant and transaminases enzymes in G. mellonella larvae. © 2009 Elsevier Inc. All rights reserved Daha fazlası Daha az

The effect of anthelmintic triclabendazole on survival and development of Galleria mellonella (Lepidoptera: Pyralidae) L. reared on artificial diet.

Kılıç, Ali | Büyükgüzel, Kemal | Büyükgüzel, Ender

Article | 2015 | KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI21 ( 6 ) , pp.841 - 847

The effect of triclabendazole, which is a benzimidazole anthelmintic, on the survival rate and developmental time in different stages of greater wax moth Galleria mellonella (L.) was investigated by rearing the larvae on the artificial diets. Triclabendazole was incorporated into diets at concentrations of 0.001, 0.01 or 0.1%. G. mellonella larvae was reared from first instar larvae to adult emergence on the artificial diets with different concentrations of triclabendazole. The survival rate in seventh instar (7th-instar) and adult stage were significantly lower at low concentrations of this anthelmintic agent (0.001 and 0.01 g per . . .100 g of diet) than the control, while there were no differences on pupation in comparison to control diet. However, the pupation and adult emergence were significantly lower at the highest dietary concentration of triclabendazole than control group but there were no differences on survival of 7th-instar larvae. The highest concentration of the anthelmintic agent (0.1 g/100 g diet) decreased pupation from 81.6 +/- 4.32% to 43.3 +/- 7.45%, adult emergence rate from 73.3 +/- 2.36 to 13.3 +/- 2.36. Triclabendazole at the highest concentration prolonged developmental time to 7th larval stage by 3.8 days reaching total 22.8 +/- 1.54 days. This diet containing 0.1% of triclabendazole significantly prolonged pupal developmental time from 24.6 +/- 1.24 days to 28.4 +/- 1.24 days, adult developmental time from 35.3 +/- 1.27 days to 42.0 +/- 1.62 days. This study is of importance in appreciation for usage of antihelmintic with different structure and mode of actions in the management of pest insects to reduce damage to environment and nontarget organisms Daha fazlası Daha az

Tuzla/Satnioeis Roma Köprüsü

Gürdal, Tayyar

Article | 2012 | Arkeoloji ve Sanat0 ( 141 ) , pp.91 - 100

Kentler ve onlara hizmet veren yerleşim dışı kutsal alanlar, özellikle Anadolu kutsal alan geleneğinin önemli özelliklerindendir. Kent ve kutsal alan arasındaki bağı kutsal bir yol sağlar. Bu kutsal yollar günlük kullanım dışında, sadece kutsal amaçlarla kullanılmışlardır. Araştırmanın konusunu oluşturan Roma Çağı köprüsü, böyle bir kutsal yolun önemli bir parçasıdır. Hellenistik ve Roma çağları boyunca Troas Bölgesi'nin en önemli kutsal alanı olan Apollon Smintheus Kutsal Alanı (Smintheion) ile anakent Aleksandreia Troas'ı birbirlerine bağlayan kutsal yol üzerinde yer alan Tuzla/Satnioeis Roma Köprüsü; antik ismi Satnioeis olan Tuz . . .la Çayı'nı aşabilmek için inşa edilmiştir. Araştırma, bütünüyle kesme taş bloklar kullanılarak inşa edilmiş olan ve Roma Çağı mimari özelliklerini yansıtan köprünün, benzer örneklerin ışığında tartışılarak tanıtılmasını amaçlamaktadır This essay aims to introduce a Roman bridge that was built on a sacred road connecting the Sanctuary of Apollo Smintheus (Smintheion) with the city of Alexandreia Troas. Smintheion was one of the most important sanctuaries of the Hellenistic period in the Troad and it was continued to be used in the succeeding Roman period. Sacred roads connecting the cities and sanctuaries are common features of ancient times. The traces of a stone paved Roman sacred road have recently been identified both at the Sminteion and Alexandreia Troas. However, the most important part of this sacred road is evidently the Roman bridge constructed on the Tuzla Stream (ancient Satnioeis). This essay in this sense aims to examine this distinctive Roman bridge in terms of architecture in light of comparable similar examples Daha fazlası Daha az

Calli ultrastructure of globularia trichosantha ssp trichosanthale

Atar, Havva | Çölgeçen, Hatice | Akgül, Gencay

Article | 2017 | PERIODICUM BIOLOGORUM119 ( 3 ) , pp.209 - 218

Background and Purpose: This study aimed to produce calli with explants of aseptic seedlings after germination of G. trichosantha ssp. trichosantha seeds by plant tissue culture method and to examine the ultrastructure of the produced calli with electron microscope preparation. Materials and Methods: Seeds of G. trichosantha ssp. trichosantha were germinated in hormone-free Murashige and Skoog in in vitro conditions. Hypocotyl, epicotyl, cotyledon, young primer leaf, apical meristem and root explants taken from 30-day aseptic seedlings were transferred to Murashige and Skoog media for callus production which contained varying concen . . .trations of 6-benzilamynopurine, indole acetic acid and 2,4 dichlorophenoxyacetic acid. Results: Two types of calli were determined: Yellow calli (Type 1) and Black calli (Type 2) with darkened colour and appearance that have not lost their development properties. Following lead staining, thin sections were examined by transmission electron microscope. The best callus production occurred at the Murashige and Skoog medium containing indole acetic acid and 6-benzilamynopurine and in root explants. The cells of Type 1 calli were spherical and large. The cells contained usually one nucleus and nucleolus. Also the cells contained a very large vacuole, endoplasmic reticulum, golgi complex, mitochondria, ribosomes, plastids. Deformed cells and spherical cells were determined in Type 2 calli. The cells were observed to have smaller vacoules and higher numbers of mitochondria different from Type 1 calli. Type 1 and Type 2 calli showed bulging mitochondrial cristae. Electrondense droplets were observed in vacuoles of both Type 1 and Type 2 calli Daha fazlası Daha az

Synthesis and characterization of poly[(RS)-3-hydroxybutyrate] telechelics and their use in the synthesis of poly(methyl methacrylate)-b-poly(3-hydroxybutyrate) block copolymers

Arslan, Hülya | Hazer, Baki | Kowalczuk, Marek

Article | 2002 | JOURNAL OF APPLIED POLYMER SCIENCE85 ( 5 ) , pp.965 - 973

Poly [(R, S)-3-hydroxybutyratel oligomers containing dihyroxyl (PHB-diol), dicarboxylic acid (PHB-diacid) and hydroxyl-carboxylic acid (a-PHB) end functionalities were obtained by the anionic polymerization of beta-butyrolacton (beta-BL). Ring opening anionic polymerization of beta-BL was initiated by a complex of 18-Crown-6 with gamma-bydroxybutyric acid sodium salts (for PHB-diol and a-PHB) or succinic acid disodium salt (for PHB-diacid). Dihydroxyl functionalization was formed by the termination of polymerization with bromo-ethanol or bromo-decanol while the others were done by protonation. Hydroxyl and/or carboxylic acid functio . . .nalized PHB oligomers with eerie salts were used to initiate the polymerization of methylmethacrylate (MXIA). PHB-b-PMMA block copolymers obtained by this way were purified by fractional precipitation and characterized using H-1-NMR and C-13-NMR, gel permeation chromatography (GPC), and thermal analysis (DSC and TGA) techniques. (C) 2002 Wiley Periodicals, Inc. J Appl Polyra Sci 85: 965-973, 2002 Daha fazlası Daha az

Anomodon longifolius (Anomodontaceae, Bryopsida) new to the bryophyte flora of Turkey

Ören, Muhammet | Uyar, Güray | Keçeli, Tamer

Note | 2010 | Turkish Journal of Botany34 ( 2 ) , pp.141 - 145

Anomodon longifolius (Schleich. ex Brid.) Hartm. is reported for the first time from Turkey, bringing the total number of Anomodon species known from Turkey to 6. This record extends its distribution range to the Western Black Sea region of Turkey. The nearest localities to Turkey for this species are in Bulgaria, Greece, the Caucasus, and Iran. This submeridional species was collected from 3 different localities (in Bolu and Barti{dotless}n provinces) in Turkey. A site description, illustrations, and diagnostic characters of the Turkish specimens are given, together with notes on its distribution and ecology. © TÜBİTAK.

Soviet Russia’s Foreign Affairs with Turkey in 1923 Reports of Ambassador Surits

Somel, Gözde

Article | 2018 | Journal of Balkan and Near Eastern Studies , pp.141 - 145

The history of Turkey–Soviet Russia relations is one of the most controversial topics in Turkish historiography. The controversy was largely a product of the Cold War interpretations when the USSR and Turkey were in hostile camps. This article shows the Bolsheviks’ reappraisal and readjustment of their policy towards Turkey during the transition of Turkey from a war of independence to the foundation of a New Turkey in conditions of peace. In doing so, the paper focuses specifically on Ambassador Surits’ detailed reports on Turkey in 1923, including his policy recommendations. In this way the article attempts to contribute to our con . . .ception of the essence of Soviet–Turkey relations by taking into account Ambassador Surits’ reports as the primary sources. © 2018, © 2018 Informa UK Limited, trading as Taylor & Francis Group Daha fazlası Daha az

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