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Tumor Necrosis Factor-Alpha (TNF-alpha-308G > A) Polymorphism in High-grade Gliomas

Ozdogan, Selcuk | Yaltirik, Cumhur Kaan | Yilmaz, Seda Gulec | Kaya, Mustafa | Duzkalir, Ali Haluk | Demirel, Nail | Kafadar, Ali

Article | 2018 | IN VIVO32 ( 2 ) , pp.287 - 289

Background/Aim: High-grade gliomas (HGG) consist of anaplastic oligoastrocytomas, anaplastic oligo-dendrogliomas, anaplastic astrocytomas and glioblastoma multiforme. The present study aimed to evaluate TNF-alpha -308 G>A polymorphism in a Turkish population. Patients and Methods: This was a prospective case-control study that included 45 patients with HGG and 49 healthy individuals. All patients were operated for intracranial tumors and the pathology results consist of high grade (Grade3 and 4) glial tumors. Results: No significant differences were found between the HGG and control groups in terms of the median age (p=0.898). There . . . were no significant differences with regard to gender (p=0.577). The TNF genotype frequency comparison between patients and controls was not statistically significant (p=0.598). Conclusion: TNF genotype frequency comparison between the patients and controls was not statistically significant in the Turkish population tested. However, further studies are needed to evaluate the genotype and phenotype correlations in large cohorts of various ethnicities Daha fazlası Daha az

Investigation of the Association between Chronic Hepatitis B and C Infection and Interleukin 2 (-330) Gene Polymorphism

Borekci, Gulay | Talin, Serap | Kandemir, Ozlem | Celik, Sevim Karakas | Berkoz, Mehmet | Aras, Nurcan

Article | 2016 | GAZI MEDICAL JOURNAL27 ( 4 ) , pp.185 - 188

Objective: Cytokines has an important role in the immunopathogenesis of hepatitis B virus (HBV) and hepatitis C virus (HCV) infections. Interleukin-2 (IL-2) secreted by Th1 cells which plays an important role in regulating both in activation of the immune system and homeostasis, is a cytokine having a wide spectrum of effects on the immune system. Although there are many studies investigating the relationship between IL-2-330 gene polymorphism and diseases, a few studied was found to investigate the role in the immunopathogenesis of HBV and HCV infections of this cytokine polymorphisms. This study was aimed to determine the relation . . .ship between IL-2-330 gene polymorphisms and chronic hepatitis B and C infections. Methods: A total of 139 patients with chronic hepatitis B, 101 patients with hepatitis C and 87 healthy subjects as control groups were included into this study. Approximately 2 ml of blood from patients and control groups were taken into tubes containing EDTA, and genomic DNA was isolated using DNA isolation kit. Single nucleotide polypmorphsim from the obtained DNAs was investigated using the polymerase chain reaction-confronting two-pair primers (PCR-CTPP) methods. Results: The genotype frequencies of IL-2-330 TT, GT, GG were detected as 23.7%, 53.2%, 23% in patients with chronic hepatitis B and 27.6%, 50.6%, 21.8% in control groups, respectively (p>0.05). The frequencies of TT, GT, GG genotypes were found to be 34.7%, 56.4%, 8.9% in patients with chronic hepatitis C and 27.6%, 50.6%, 21.8% in control group, respectively. GG genotype frequency was significantly lower in patient groups with hepatitis C compared with the control group (p0.05). The frequencies of T and G alleles were found to be 69.4%, 30.6% in patients with chronic hepatitis C and 52.9%, 47.1% in control groups, respectively ( Daha fazlası Daha az

Functional association of interleukin-18 gene -607 C/A promoter polymorphisms with endometriosis

Ayaz L. | Çelik S.K. | Çayan F. | Aras-Ate N. | Tamer L.

Article | 2011 | Fertility and Sterility95 ( 1 ) , pp.298 - 300

This study evaluated for the first time the relationship between interleukin-18 (IL-18) C607A genotypes and endometriosis in 135 women with endometriosis and 84 controls. In the study population, IL-18 -607A homozygote and A allele were positively correlated with the risk of developing endometriosis or the stage of endometriosis. © 2011 American Society for Reproductive Medicine, Published by Elsevier Inc.

The Ala-9Val polymorphism in the mitochondrial targeting sequence (MTS) of the manganese superoxide dismutase gene is not associated with juvenile-onset asthma

Gurel, A | Tomac, N | Yilmaz, HR | Tekedereli, I | Akyol, O | Armutcu, F | Yuce, H

Article | 2004 | CLINICAL BIOCHEMISTRY37 ( 12 ) , pp.1117 - 1120

Background: We aimed to investigate the possible association between Mn-SOD polymorphism in the mitochondrial targeting sequence and asthma. Methods: Alanine or valine polymorphism in the signal peptide of Mn-SOD gene was evaluated using a primer pair to amplify a 107-bp fragment followed by digestion with NgoM IV Results: No significant difference in genotype frequencies was found between patients and controls. Conclusion: These results suggest no major modifying role for the Mn-SOD gene polymorphism in patients with asthma. (C) 2004 The Canadian Society of Clinical Chemists. All rights reserved.

Relationship between FokI polymorphism in the vitamin D receptor gene and fibromyalgia syndrome

Maraslı E. | Ozdolap S. | Sarıkaya S.

Article | 2016 | International Journal of Rheumatic Diseases19 ( 11 ) , pp.1063 - 1068

Aim: The aim of this study was to evaluate the vitamin D receptor (VDR) gene FokI polymorphism frequencies distribution in subjects with fibromyalgia syndrome (FMS) compared to healthy controls. Method: Using a case-control design, 100 female patients, who were diagnosed with FMS according to the American College of Rheumatology criteria and 100 healthy female subjects were enrolled in this study. FokI polymorphisms of the VDR gene were analyzed by restriction fragment length polymorphisms (RFLP) in both groups. Results: No significant differences in the frequencies distribution of both genotype and alleles of the FokI polymorphism . . .in the VDR gene were observed between the two groups. Conclusion: The relationship between VDR gene FokI polymorphism and FMS, particularly in Turkish women, could not be determined in this study. However, further studies with larger patient numbers may be needed to prove a relation between VDR gene polymorphism and FMS. © 2016 Asia Pacific League of Associations for Rheumatology and John Wiley & Sons Australia, Lt Daha fazlası Daha az

Increased Serum Levels of IL-28 and IL-29 and the Protective Effect of IL28B rs8099917 Polymorphism in Patients with Hashimoto’s Thyroiditis

Arpaci D. | Karakas Celik S. | Can M. | Cakmak Genc G. | Kuzu F. | Unal M. | Bayraktaroglu T.

Article | 2016 | Immunological Investigations45 ( 7 ) , pp.668 - 678

Hashimoto’s thyroiditis (HT) is thought to result from decreased T helper type 2 (Th2) responses, leading to the progressive destruction of thyrocytes. IFN-?1, -?2, and -?3 (also known as IL-29, IL-28A, and IL-28B, respectively) are recently described members of the IFN-? family and have been shown to decrease the production of Th2 cytokines in vitro. However, the role and mechanism of IFN-?1 in HT remain unknown. The purpose of this study was to examine whether IL29 and IL28B gene polymorphisms are susceptibility genes for the development of HT. Also, we investigated the effects of IL-29 and IL-28 serum levels in the pathogenesis o . . .f HT. Using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method, single-nucleotide polymorphisms (SNPs) of IL28B rs8099917 (IL28 G/T) and IL29 rs30461 (IL29 T/C) were studied in 99 patients with HT and 100 healthy controls. Considering the allelic distribution of the IL28 G/T polymorphism, a higher frequency of the G allele was observed in the control group versus the HT group. Thus, it was suggested that the G allele may be protective against HT pathogenesis (OR = 0.388, 95% CI = 0.217–0.693; p = 0.001). Our findings also demonstrated that there was a statistically significant difference in serum IL-28 and IL-29 levels between case and control groups (p < 0.001). Increased serum levels of IL-28 and IL-29 were found in patients with HT. However, we did not find a relationship between the IL29 gene polymorphism and HT. In conclusion, the IL28B gene polymorphism and serum IL-28 and IL-29 levels seem to play a role in the pathogenesis of HT. © 2016 Taylor & Francis Daha fazlası Daha az

Investigation of the Association Between Chronic Hepatitis B and C Infections and TNF-alpha(-308) Gene Polymorphism

Borekci, Gulay | Aras, Nurcan | Kandemir, Ozlem | Yalin, Serap | Celik, Sevim Karakas | Berkoz, Mehmet

Article | 2016 | MIKROBIYOLOJI BULTENI50 ( 2 ) , pp.236 - 244

Cytokines and genetic factors play important roles in the pathogenesis of chronic hepatitis B (CHB) and chronic hepatitis C (CHC) infections. Variations in cytokine genes may effect the gene expression and may lead to changes in the clinical manifestations of diseases. One of the single nucleotide polymorphisms in the promoter region of tumor necrosis factor-alpha (TNF-alpha) gene is the polymorphism at -308. position which was investigated in many studies by means of its relationship between CHB and CHC infections, however their results are incompatible. Furthermore, there is no sufficient data on this subject in our country. This . . .study was aimed to determine the relationship between TNF-a(-308) gene polymorphism with CHB and CHC infections. A total of 271 patients with chronic hepatitis and 181 healthy subjects were included in the study. Of them 167 were CHB cases (67 female, 100 male; age range 18-74 years, mean age: 40.23 +/- 13.09) and 95 controls for CHB group (46 female, 49 male; mean age: 36.41 +/- 15.0 years), while 104 were CHC cases (63 female, 41 male; age range: 25-79 years, mean age: 52.8 +/- 12.6) and 86 controls for CHC group (41 female, 45 male; mean age: 36.4 +/- 14.9 years). After the isolation of genomic DNA from blood samples of the patient and control groups, TNF-alpha(-308)G/A (rs 1800629) polymorphism was investigated by using the real-time polymerase chain reaction from the obtained DNAs. Among CHB group, TNF-alpha(-308) GG, GA, AA genotypes were detected in 126 (75.4%), 38 (22.8%) and 3 (1.8%) of the patients, respectively, while these numbers were 84 (88.4%), 11 (11.6%) and 0 (0%) in control group, respectively. Among CHC group, TNF-a(-308) GG, GA, AA genotypes were detected in 37 (35.6%), 28 (26.9%) and 39 (37.5%) of the patients, respectively, while these numbers were 38 (44.2%), 8 (9.3%) and 40 (46.5%) in control group, respectively. The frequency of GA genotype was significantly higher in both patient groups compared to the control groups (p=0.024 for CHB and p= 0.006 for CHC). When the distribution of allele frequencies of TNF-alpha(-308)G/A polymorphism was evaluated in the patients and control groups, it was noted that G allele was found to be high in CHB patients comparing with controls (94.2% vs 86.8%), however A allele was identified to be lower than controls (5.8% vs 13.2%) (p= 0.008). In contrast, there was no significant difference in terms of allele frequency compared with CHC patients and the control group (p= 0.969). In conclusion, our data in accordance with the results of many studies in literature, determined that TNF-alpha(-308) polymorphisms can influence the chronicity of hepatitis B and C infections. Further studies on this subject would contribute to the elucidation of the molecular mechanisms of chronic hepatitis B and C diseases Daha fazlası Daha az

Increased Serum Levels of IL-28 and IL-29 and the Protective Effect of IL28B rs8099917 Polymorphism in Patients with Hashimoto's Thyroiditis

Arpaci, Dilek | Celik, Sevim Karakas | Can, Murat | Genc, Gunes Cakmak | Kuzu, Fatih | Unal, Mustafa | Bayraktaroglu, Taner

Article | 2016 | IMMUNOLOGICAL INVESTIGATIONS45 ( 7 ) , pp.668 - 678

Hashimoto's thyroiditis (HT) is thought to result from decreased T helper type 2 (Th2) responses, leading to the progressive destruction of thyrocytes. IFN-1, -2, and -3 (also known as IL-29, IL-28A, and IL-28B, respectively) are recently described members of the IFN- family and have been shown to decrease the production of Th2 cytokines in vitro. However, the role and mechanism of IFN-1 in HT remain unknown. The purpose of this study was to examine whether IL29 and IL28B gene polymorphisms are susceptibility genes for the development of HT. Also, we investigated the effects of IL-29 and IL-28 serum levels in the pathogenesis of HT. . . . Using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method, single-nucleotide polymorphisms (SNPs) of IL28B rs8099917 (IL28 G/T) and IL29 rs30461 (IL29 T/C) were studied in 99 patients with HT and 100 healthy controls. Considering the allelic distribution of the IL28 G/T polymorphism, a higher frequency of the G allele was observed in the control group versus the HT group. Thus, it was suggested that the G allele may be protective against HT pathogenesis (OR = 0.388, 95% CI = 0.217-0.693; p = 0.001). Our findings also demonstrated that there was a statistically significant difference in serum IL-28 and IL-29 levels between case and control groups (p < 0.001). Increased serum levels of IL-28 and IL-29 were found in patients with HT. However, we did not find a relationship between the IL29 gene polymorphism and HT. In conclusion, the IL28B gene polymorphism and serum IL-28 and IL-29 levels seem to play a role in the pathogenesis of HT Daha fazlası Daha az

Relationship Between TLR2 and TLR4 Gene Polymorphisms with Psoriasis

Keni, Fatih Mehmet | Celik, Sevim Karakas | Solak, Nilgun | Genc, Gunes Cakmak | Dursun, Ahmet

Article | 2018 | TURK DERMATOLOJI DERGISI-TURKISH JOURNAL OF DERMATOLOGY12 ( 1 ) , pp.28 - 32

Objective: Psoriasis is a common, chronic and recurrent disease which can affect skin and also joints. Although the etiopathogenesis of psoriasis has not precisely determined, the most supported mechanism is inflammation triggered by any factor. Toll like receptors (TLRs) family described in recent years is known to play a critical role in host immunity against a wide variety of pathogens. In our study, we aimed to reveal possible relationships of some TLR gene polymorphisms with psoriasis in this patient group. Methods: A hundred patients who diagnosed with psoriasis and 173 healthy controls were included in the study which known t . . .o be without inflammatory disease, TLR2 gene Arg677Trp, Arg753Gln, -196-174 del and TLR4 gene Asp299Gly, Thr399Ile polymorphisms were determined by polymerase chain reaction-restriction fragment length polymorphism method, patient and control groups were compared in terms of gene polymorphisms mentioned. Results: In this study, it was determined psosiasis has a statistically significant relationship with GA genotype and A allele in TLR2 Arg753Gln polymorphism. Furthermore, when the patient and control groups were compared for -196-174 del gene polymorphism, it was determined that ins/del genotype had a protective effect. Conclusion: We think that variant alleles in the TLR2 gene may play an important role in the molecular etiopathogenesis of psoriasis Daha fazlası Daha az

Functional association of interleukin-18 gene-607 C/A promoter polymorphisms with endometriosis

Ayaz, Lokman | Celik, Sevim Karakas | Cayan, Filiz | Aras-Ates, Nurcan | Tamer, Lulufer

Editorial | 2011 | FERTILITY AND STERILITY95 ( 1 ) , pp.298 - 300

This study evaluated for the first time the relationship between interleukin-18 (IL-18) C607A genotypes and endometriosis in 135 women with endometriosis and 84 controls. In the study population, IL-18 -607*A homozygote and A allele were positively correlated with the risk of developing endometriosis or the stage of endometriosis. (Fertil Steril (R) 2011; 95:298-300. (c) 2011 by American Society for Reproductive Medicine.)

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