Ulker, A. Evren | Tulunoglu, Oezlem | Ozmeric, Nurdan | Can, Murat | Demirtas, Selda

Article | 2008 | JOURNAL OF PERIODONTOLOGY79 ( 5 ) , pp.854 - 860

Background: The aim of this study was to evaluate the levels of cystatin C, interleukin-1 beta (IL-1 beta), and tumor necrosis factor-alpha (TNF-alpha) in the total saliva and gingival crevicular fluid (GCF) of periodontally healthy children (PHC) and children with gingivitis (CG) who were between I I and 16 years old. Methods: The study was carried out with 10 PHC and 25 CG. Unstimulated total saliva and GCF samples were obtained. Clinical parameters, including probing depth (PD), clinical attachment loss (CAL), plaque index (PI), gingival index (GI), and gingival bleeding index (GBI), were assessed. GCF samples were collected from . . . four maxillary upper incisors. After sampling, biochemical analyses were performed using latex particle-enhanced turbidimetric immunoassay for cystatin C and enzyme-linked immunosorbent assay for IL-1 beta and TNF-alpha. The multivariate analysis of variance test was used for statistical evaluation. Results: In total saliva, cystatin C and TNF-a levels were higher in PHC, and IL-1 beta levels were higher in CG, but the differences were not statistically significant. In GCF, cystatin C levels were higher in PHC (P >0.05), whereas TNF-alpha and IL-1 beta levels were higher in CG (P >0.05). In the CG group, there were positive correlations between the GCF cystatin C level and the PI of the sampled site (r = 0.488; P Daha fazlası Daha az

Balli U. | Ongoz Dede F. | Bozkurt Dogan S. | Gulsoy Z. | Sertoglu E.

Article | 2016 | Oral Diseases22 ( 7 ) , pp.673 - 680

Objective: To investigate changes in the levels of gingival crevicular fluid (GCF) chemerin and interleukin-6 (IL-6) in both obese/non-obese individuals with periodontitis following non-surgical periodontal therapy. Methods: Individuals (n = 80) were split into four groupings according to periodontal/anthropometric parameters: (i) periodontal healthy without obesity; (ii) chronic periodontitis (CP) without obesity; (iii) periodontal healthy with obesity; and (iv) CP with obesity. Individuals with periodontitis were treated with non-surgical periodontal therapy. Both GCF sampling procedures and clinical periodontal measures were perf . . .ormed prior to treatment and 6 weeks thereafter. Enzyme-linked immunosorbent assay was utilized to measure both chemerin and IL-6 levels. Results: Greater values for chemerin and IL-6 were observed in obese individuals compared to their non-obese controls and in individuals with CP compared to their periodontal healthy controls (P Daha fazlası Daha az

Öngöz Dede F. | Balli U. | Bozkurt Doğan Ş. | Güven B.

Article | 2017 | Journal of Periodontal Research52 ( 3 ) , pp.397 - 407

Background and Objective: The cytokine, interleukin (IL)-32, is a relatively new discovery. However, it is very powerful for stimulating tumor necrosis factor-alpha (TNF-?) under inflammatory conditions. The objective of this research was to explore fluctuations in the levels of TNF-?, IL-32 and IL-10, in both saliva and gingival crevicular fluid. The focus was on measurements taken before and after clinical treatment of chronic periodontitis. Material and Methods: For the purposes of the study, a total of 27 patients with chronic periodontitis and 27 controls (periodontally healthy) were recruited. Important clinical periodontal cr . . .iteria were established before and 4 wk after the start of the research. The chronic periodontitis group was given an initial form of periodontal care. Samples of saliva and gingival crevicular fluid were collected exactly 4 wk preceding and 4 wk following the care. The levels of IL-10, IL-32 and TNF-? present in saliva and gingival crevicular fluid were recorded via the use of an ELISA. Results: At baseline, the levels of TNF-? and IL-32 in the gingival crevicular fluid and saliva were significantly higher among patients in the chronic periodontitis group than among patients in the control group (p < 0.05). On the other hand, at baseline the levels of IL-10 were significantly lower in the gingival crevicular fluid and saliva of the chronic periodontitis group than the control group (p < 0.05). A significantly positive link was found between the TNF-? and IL-32 levels in the two study groups (p < 0.05). After treatment, the levels of TNF-? and IL-32 in saliva and gingival crevicular fluid were significantly lower in the chronic periodontitis group when compared with the baseline readings. However, the levels of IL-10 were significantly higher (p < 0.05). Conclusion: Ultimately, the level of IL-32 present in saliva and gingival crevicular fluid might be useful as an indicator of the condition and the expectations for its treatment and care. According to the results of the research, the proinflammatory impact of IL-32 could potentially be linked to the intensity and progression of periodontitis. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Lt Daha fazlası Daha az

Türer Ç.C. | Ballı U. | Güven B.

Article | 2017 | Oral Diseases23 ( 3 ) , pp.379 - 386

Objectives: This study is evaluating fetuin-A, serum amyloid A (SAA) and tumor necrosis factor alpha (TNF-?) levels in gingival crevicular fluid (GCF) and serum samples in periodontal health and disease. Material and Methods: Sixty patients were divided into three groups: Group 1 periodontal health (n = 20), Group 2 gingivitis (n = 20) and Group 3 chronic periodontitis (CP) (n = 20). GCF and serum samples were evaluated using enzyme-linked immunosorbent assay kit. Results: SAA and TNF-? levels in GCF and serum were significantly higher in patients with gingivitis and CP compared with controls (P