Filtreler
The effects of quercetin on bone minerals, biomechanical behavior, and structure in streptozotocin-induced diabetic rats

Kanter M. | Altan M.F. | Donmez S. | Ocakci A. | Kartal M.E.

Article | 2007 | Cell Biochemistry and Function25 ( 6 ) , pp.747 - 752

This study was designed to investigate the effect of quercetin (QE) on bone minerals and biomechanics in insulin-dependent diabetic rats. Diabetes was induced by 50 mg kg-1 intraperitoneal streptozotocin (STZ) in a single dose. The rats were randomly allotted into four experimental groups: A (control), B (non-diabetic + QE), C (diabetic), and D (diabetic + QE) each containing 10 animals. The diabetic rats received QE (15 mg kg-1 day-1) for 4 weeks following 8 weeks of STZ injection. Blood samples were taken to determine glucose, insulin, calcium, and magnesium levels. The rats' femora were assessed biomechanically at femoral mid-dia . . .physis and neck. It was found that QE treatment increased insulin, calcium, and magnesium levels. Three-point bending of the femoral mid-diaphysis and necks showed significantly lower maximum load values (Fmax) in animals in the STZ group than the QE + STZ or control groups (p < 0.05). The results support the conclusion that QE treatment may decrease blood glucose and increase plasma insulin, calcium, and magnesium. QE treatment may also be effective in bone mineral metabolism, biomechanical strength, and bone structure in STZ-induced diabetic rats. Copyright © 2007 John Wiley & Sons, Ltd Daha fazlası Daha az

Combination therapy of Nigella sativa and human parathyroid hormone on bone mass, biomechanical behavior and structure in streptozotocin-induced diabetic rats

Altan M.F. | Kanter M. | Donmez S. | Kartal M.E. | Buyukbas S.

Article | 2007 | Acta Histochemica109 ( 4 ) , pp.304 - 314

Extracts of the seeds of Nigella sativa (NS), an annual herbaceous plant of the Ranunculaceae family, have been used for many years for therapeutic purposes, including their potential anti-diabetic properties. The aim of the present study was to test the hypothesis that combined treatment with NS and human parathyroid hormone (hPTH) is more effective than treatment with NS or hPTH alone in improving bone mass, connectivity, biomechanical behaviour and strength in insulin-dependent diabetic rats. Diabetes was induced by intraperitoneal injection of streptozotocin (STZ) at a single dose of 50 mg/kg. The diabetic rats received NS (2 ml . . ./kg/day, i.p.), hPTH (6 µg/kg/day, i.p.) or NS and hPTH combined for 4 weeks, starting 8 weeks after STZ injection. The ß-cells of the pancreatic islets of Langerhans were examined by immunohistochemical methods. In addition, bone sections of femora were processed for histomorphometry and biomechanical analysis. In diabetic rats, the ß-cells were essentially negative for insulin-immunoreactivity. NS treatment (alone or in combination with hPTH) significantly increased the area of insulin immunoreactive ß-cells in diabetic rats; however, hPTH treatment alone only led to a slightly increase in the insulin-immunoreactivity. These results suggest that NS might be used in a similar manner to insulin as a safe and effective therapy for diabetes and might be useful in the treatment of diabetic osteopenia. © 2007 Elsevier GmbH. All rights reserved Daha fazlası Daha az

The use of Alloxan and Streptozotocin in Experimental Diabetes Models [Deneysel Diyabet Modellerinde Alloksan ve Streptozotosin Kullanımı]

Kurçer Z. | Karaoglu D.

Review | 2012 | Turkish Journal of Endocrinology and Metabolism16 ( 2 ) , pp.34 - 40

Diabetes is a chronic metabolic disease which leads to several acute and chronic complications, morbidity and mortality, and decreased lifespan and quality of life. Therefore, in research studies that aim to enlighten the pathogenesis of diabetes and investigate possible treatment strategies, experimental animal models of diabetes provide many advantages to the investigator. Models of diabetes obtained by chemical induction, diet, surgical manipulations or combination thereof and also new genetically modified animal models are some of the experimental models. Alloxan and streptozotocin (STZ), which are toxic glucose analogues that p . . .referentially accumulate in pancreatic beta cells, are widely used toxic agents to induce experimental diabetes in animals. This review gives an overview on the use of alloxan and STZ to induce chemical diabetes models with reference to their mechanisms, utilizable doses, advantages and disadvantages in diabetes research Daha fazlası Daha az

Exercise training prevents and protects streptozotocin-induced oxidative stress and ß-cell damage in rat pancreas

Coskun O. | Ocakci A. | Bayraktaroglu T. | Kanter M.

Article | 2004 | Tohoku Journal of Experimental Medicine203 ( 3 ) , pp.145 - 154

The aim of the present study was the evaluation of possible protective effects of exercise against ß-cell damage in streptozotocin (STZ)-induced diabetes in rats. The animals were divided into five groups: the control group, the STZ-induced diabetes group, the STZ-induced diabetes and light-intensity exercise group, the STZ-induced diabetes and moderate-intensity exercise group, and the STZ-induced diabetes and heavy-intensity exercise group. Animals in the exercise groups were made to swim one of three exercise protocols once a day for 12 consecutive weeks. STZ was injected intraperitoneally at a single dose of 50 mg/kg for diabete . . .s induction. Exercise training was continued for 4 weeks prior to STZ administration; these applications were continued end of the study (for 12 weeks). Erythrocyte and pancreatic tissue malondialdehyde (MDA) levels and serum nitric oxide (NO) concentration were measured. Moreover glutathione peroxidase (GSHPx), superoxide dismutase (SOD) and catalase (CAT) were also measured in pancreatic homogenates. Pancreatic ß-cells were examined by immunohistochemical methods. STZ increased lipid peroxidation and decreased the antioxidant enzyme activity significantly. Exercise, especially moderate-intensity exercise has shown protective effect probably through decreasing lipid peroxidation and increasing antioxidant enzyme activity. Islet cell degeneration and weak insulin immunohistochemical staining were observed in STZ induced diabetic rats. Increased intensity of staining for insulin and preservation of ß-cell numbers were apparent in the exercise-applied diabetic rats. Interestingly, the best result was obtained from moderate-intensity exercise. These findings suggest that exercise has a therapeutic and/or protective effect in diabetes by decreasing oxidative stress and preservation of pancreatic ß-cell integrity. © 2004 Tohoku University Medical Press Daha fazlası Daha az

Quercetin, a flavonoid antioxidant, prevents and protects streptozotocin-induced oxidative stress and ß-cell damage in rat pancreas

Coskun O. | Kanter M. | Korkmaz A. | Oter S.

Article | 2005 | Pharmacological Research51 ( 2 ) , pp.117 - 123

The aim of the present study was the evaluation of possible protective effects of quercetin (QE) against ß-cell damage in experimental streptozotocin (STZ)-induced diabetes in rats. STZ was injected intraperitoneally at a single dose of 50 mg kg -1 for diabetes induction. QE (15 mg kg -1 day, intraperitoneal (i.p.) injection) was injected for 3 days prior to STZ administration; these injections were continued to the end of the study (for 4 weeks). It has been believed that oxidative stress plays a role in the pathogenesis of diabetes mellitus (DM). In order to determine the changes of cellular antioxidant defense system, antioxidant . . . enzymes such as glutathione peroxidase (GSHPx), superoxide dismutase (SOD) and catalase (CAT) activities were measured in pancreatic homogenates. Moreover we also measured serum nitric oxide (NO) and erythrocyte and pancreatic tissue malondialdehyde (MDA) levels, a marker of lipid peroxidation, if there is an imbalance between oxidant and antioxidant status. Pancreatic ß-cells were examined by immunohistochemical methods. STZ induced a significant increase lipid peroxidation, serum NO concentrations and decreased the antioxidant enzyme activity. Erythrocyte MDA, serum NO and pancreatic tissue MDA significantly increased (P < 0.05) and also the antioxidant levels significantly decreased (P < 0.05) in diabetic group. QE treatment significantly decreased the elevated MDA and NO (P < 0.05), and also increased the antioxidant enzyme activities (P < 0.05). QE treatment has shown protective effect possibly through decreasing lipid peroxidation, NO production and increasing antioxidant enzyme activity. Islet cells degeneration and weak insulin immunohistochemical staining was observed in STZ induced diabetic rats. Increased staining of insulin and preservation of islet cells were apparent in the QE-treated diabetic rats. These findings suggest that QE treatment has protective effect in diabetes by decreasing oxidative stress and preservation of pancreatic ß-cell integrity. © 2004 Elsevier Ltd. All rights reserved Daha fazlası Daha az

Effects of Nigella sativa on oxidative stress and ß-cell damage in streptozotocin-induced diabetic rats

Kanter M. | Coskun O. | Korkmaz A. | Oter S.

Article | 2004 | Anatomical Record - Part A Discoveries in Molecular, Cellular, and Evolutionary Biology279 ( 1 ) , pp.685 - 691

The aim of the present study was to evaluate the possible protective effects of Nigella sativa L. (NS) against ß-cell damage from streptozotocin (STZ)-induced diabetes in rats. STZ was injected intraperitoneally at a single dose of 50 mg/kg to induce diabetes. NS (0.2 ml/kg/day, i.p.) was injected for 3 days prior to STZ administration, and these injections were continued throughout the 4-week study. Oxidative stress is believed to play a role in the pathogenesis of diabetes mellitus (DM). To assess changes in the cellular antioxidant defense system, we measured the activities of antioxidant enzymes (such as glutathione peroxidase ( . . .GSHPx), superoxide dismutase (SOD), and catalase (CAT)) in pancreatic homogenates. We also measured serum nitric oxide (NO) and erythrocyte and pancreatic tissue malondialdehyde (MDA) levels, a marker of lipid peroxidation, to determine whether there is an imbalance between oxidant and antioxidant status. Pancreatic ß-cells were examined by immunohistochemical methods. STZ induced a significant increase in lipid peroxidation and serum NO concentrations, and decreased antioxidant enzyme activity. NS treatment has been shown to provide a protective effect by decreasing lipid peroxidation and serum NO, and increasing antioxidant enzyme activity. Islet cell degeneration and weak insulin immunohistochemical staining was observed in rats with STZ-induced diabetes. Increased intensity of staining for insulin, and preservation of ß-cell numbers were apparent in the NS-treated diabetic rats. These findings suggest that NS treatment exerts a therapeutic protective effect in diabetes by decreasing oxidative stress and preserving pancreatic ß-cell integrity. Consequently, NS may be clinically useful for protecting ß-cells against oxidative stress. © 2004 Wiley-Liss, Inc Daha fazlası Daha az

6698 sayılı Kişisel Verilerin Korunması Kanunu kapsamında yükümlülüklerimiz ve çerez politikamız hakkında bilgi sahibi olmak için alttaki bağlantıyı kullanabilirsiniz.

creativecommons
Bu site altında yer alan tüm kaynaklar Creative Commons Alıntı-GayriTicari-Türetilemez 4.0 Uluslararası Lisansı ile lisanslanmıştır.
Platforms