Bulunan: 45 Adet 0.002 sn
Koleksiyon [13]
Tam Metin [2]
Yayın Türü [3]
Yazar [20]
Yayın Yılı [19]
Tez Danışmanı [2]
Konu Başlıkları [20]
Yayıncı [18]
Yayın Dili [3]
Dergi Adı [20]
Gastroprotective activity of Nigella sativa L oil and its constituent, thymoquinone against acute alcohol-induced gastric mucosal injury in rats

Kanter, Mehmet | Demir, Halit | Karakaya, Cengiz | Ozbek, Hanefi

Article | 2005 | WORLD JOURNAL OF GASTROENTEROLOGY11 ( 42 ) , pp.6662 - 6666

AIM: To evaluate the role of reactive oxygen species in the pathogenesis of acute ethanol-induced gastric mucosal lesions and the effect of Nigella sativa L oil (NS) and its constituent thymoquinone (TQ) in an experimental model. METHODS: Male Wistar albino rats were assigned into 4 groups. Control group was given physiologic saline orally (10 mL/kg body weight) as the vehicle (gavage); ethanol group was administrated 1 mL (per rat) absolute alcohol by gavage; the third and fourth groups were given NS (10 mL/kg body weight) and TQ (10 mg/kg body weight p.o) respectively 1 h prior to alcohol intake. One hour after ethanol administrat . . .ion, stomach tissues were excised for macroscopic examination and biochemical analysis. RESULTS: NS and TQ could protect gastric mucosa against the injurious effect of absolute alcohol and promote ulcer healing as evidenced from the ulcer index (UI) values. NS prevented alcohol-induced increase in thiobarbituric acid-reactive substances (TBARS), an index of lipid peroxidation. NS also increased gastric glutathione content (GSH), enzymatic activities of gastric superoxide dismutase (SOD) and glutathione-S-transferase (GST). Likewise, TQ protected against the ulcerating effect of alcohol and mitigated most of the biochemical adverse effects induced by alcohol in gastric mucosa, but to a lesser extent than NS. Neither NS nor TQ affected catalase activity in gastric tissue. CONCLUSION: Both NS and TQ, particularly NS can partly protect gastric mucosa from acute alcohol-induced mucosal injury, and these gastroprotective effects might be induced, at least partly by their radical scavenging activity. (C) 2005 The WJG Press and Elsevier Inc. All rights reserved Daha fazlası Daha az

The effects of photoperiod on testes in rat: A morphometric and immunohistochemical study

Kus I. | Akpolat N. | Oner H. | Ayar A. | Pekmez H. | Ozen O.A. | Sarsilmaz M.

Article | 2003 | Neuroendocrinology Letters24 ( 03.Apr ) , pp.209 - 214

Objective: The aim of this study was to examine the effects of photoperiod on testes in rat. Design: For this purpose 21 male Wistar rats were used. Animals were divided into three groups. Control rats in group I were kept under 12 hrs light: 12 hrs dark conditions (12L: 12D) for 10 weeks. Animals in group II were exposed to long photoperiods (18L: 6D), while rats in group III were exposed to short photoperiods (6L: 18D) for 10 weeks. At the end of the experiment, all animals were killed by decapitation and blood samples were obtained. Serum testosterone levels were determined with the use of a chemiluminescent enzyme immunoassay. T . . .he testes of all rats were removed and weighed. Testicular tissue was processed semiquantitative evaluation of immunohistochemical testosterone staining. Intensity of immunostaining was determined on a scale between 0 (no staining) and 5 (heavy staining). For morphometric comparison, diameters of seminiferous tubules in each group were measured. Results: In rats exposed to long photoperiods, testicular weights, diameters of seminiferous tubules and serum testosterone levels were significantly increased as compared to those in control rats. Whereas, exposure of rats to short photoperiods resulted in significantly decrease of testicular weights, diameters of seminiferous tubules and serum testosterone levels as compared to those in control rats and rats maintained in long photoperiods. Immunostaining of testosterone was moderate (3+) in control rats, heavy (5+) in rats exposed to long photoperiods and minimal (1+) in rats exposed to short photoperiods. Conclusions: The results of our study indicate that testicular functions increase after exposure to long photoperiods and decrease after exposure to short photoperiods Daha fazlası Daha az

The effect of locally administered organic silicon on calvarial bone defects

Turer, Akif | Onger, Mehmet Emin


Aim: The purpose of this study was to investigate the potential of the local administration of organic silicon on autogenous grafted critical-sized cortical bone defects. Material and Method: Twenty-four rats were divided into three groups: group C (passive control), group Au, and group Si. A 5-mm diameter critical-size defect was created in the calvarium of each rat. In group C, only a sterile saline-treated absorbable collagen sponge was applied to the defect area. In group Au, autogenous graft was performed and sterile saline-treated absorbable collagen sponge was applied on the autografted defect area. In group Si, autogenous gr . . .aft was performed and a 500 mg silicon-treated absorbable collagen sponge was applied to the autografted defect area. All animals were euthanized at 28 days postoperative. Stereologic analyses were performed. New bone area and connective tissue volumes were measured. Results: Stereologic analysis showed that the difference between group Si with a mean bone formation of 1.79 +/- 0.48 mm3 and groups Au and C was statistically significant (p Daha fazlası Daha az

Effects of human placental amnion derived mesenchymal stem cells on proliferation and apoptosis mechanisms in chronic kidney disease in the rat

Cetinkaya B. | Unek G. | Kipmen-Korgun D. | Koksoy S. | Korgun E.T.

Article | 2019 | International Journal of Stem Cells12 ( 1 ) , pp.151 - 161

Background and Objectives: The feature of chronic kidney failure (CKF) is loss of kidney functions due to erosion of healthy tissue and fibrosis. Recent studies showed that Mesenchymal stem cells (MSCs) differentiated into tubular epithelial cells thus renal function and structures renewed.Furthermore, MSCs protect renal function in CKF. Therefore, we aimed to investigate whether human amnion-derived mesenchymal stem cells (hAMSCs) can repair fibrosis and determine the effects on proliferation and apoptosis mechanisms in chronic kidney failure. Methods and Results: In this study, rat model of CKF was constituted by applying Aristolo . . .chic acid (AA). hAMSCs were isolated from term placenta amnion membrane and transplanted into tail vein of rats. At the end of 30 days and 60 days of recovery period, we examined expressions of PCNA, p57 and Parp-1 by western blotting. Immunoreactivity of PCNA, Ki67, IL-6 and Collagen type I were detected by immunohistochemistry. Besides, apoptosis was detected by TUNEL. Serum creatinine and urea were measured. Expressions of PCNA and Ki67 increased in hAMSC groups compared with AA group. Furthermore, expressions of PARP-1 apoptosis marker and p57 cell cycle inhibitory protein increased in AA group significantly according to control, hAMSC groups and sham groups. IL-6 proinflammatory cytokine increased in AA group significantly according to control, hAMSCs groups and sham groups. Expressions of Collagen type I protein reduced in hAMSCs groups compared to AA group. After hAMSC treatment, serum creatinine and urea levels significantly decreased compared to AA group. After injection of hAMSC to rats, Masson's Trichrome and Sirius Red staining showed fibrosis reduction in kidney. Conclusions: According to our results hAMSCs can be ameliorate renal failure. © 2019 by the Korean Society for Stem Cell Research Daha fazlası Daha az

Stereological evaluation of local administered palmatine on bone regeneration [Lokal olarak uygulanan palmatinin kemik rejenerasyonuna etkisinin stereolojik olarak incelenmesi]

Türer Ç.C. | Türer A.

Article | 2017 | Journal of Clinical and Analytical Medicine8 ( 6 ) , pp.478 - 481

Aim: The purpose of this study is to investigate the healing potential of the local administration of palmatine on autogenous grafted critical-sized cortical bone defects. Material and Method: Twenty-four rats were divided into three groups: Group C (passive control), Group G (active control) and Group G+PLM. A 5-mm diameter critical-size defect was created in the calvarium of each animal. In Group C, the defects were left empty. In Group G defects were filled with only autogenous graft and an absorbable collagen sponge treated with 300 mg sterile saline solution was applied on grafted area. In Group G+PLM defects were filled with a . . .utogenous graft, and 300 mg palmatine treated absorbable collagen sponge was applied on grafted area. All animals were euthanized at 28 days postoperative. Stereologic analyses were performed. New bone volume and connective tissue volumes were measured. Results: Stereologic analysis showed that Group G and G+PLM significantly had more new bone at four weeks compared with group C. Connective tissue volumes were also significantly higher in autografted groups. New bone and connective tissue volumes’ difference were not statistically significant between group G and G+PLM groups. Discussion: Locally administered 500 mg palmatine doesn’t enhance bone regeneration in critical size calvarial rat defects filled with autologous graft. © 2017, Derman Medical Publishing. All rights reserved Daha fazlası Daha az

Effects of estradiol benzoate on the ultrastructure of the pinealocyte in the ovariectomized rat

Kus I. | Oner H. | Ozogul C. | Ayar A. | Ozen O.A. | Sarsilmaz M. | Kelestimur H.

Article | 2002 | Neuroendocrinology Letters23 ( 05.Jun ) , pp.405 - 410

Objective: The aim of this study was to examine the effects of ovariectomy and ovariectomy followed by estradiol benzoate administration on the ultrastructure of pinealocytes in female rat. Design: For this purpose 15 female Wistar rats were used. Animals were divided into three groups. Group I and II were designated as sham-ovariectomized (control) and ovariectomized, respectively. Group III was ovariectomized and daily injected with estradiol benzoate for one month. At the end of the experiment, all animals were anesthetized with ketamine for fixation by vascular perfusion. Pineal glands of groups I, II and III were removed. All s . . .pecimens were examined by electron microscopy. Results: Ovariectomy caused an increase of lipid droplets, mitochondria and ribosomes. Rough endoplasmic reticulum was extensive in the cytoplasm. Estradiol administration to ovariectomized rats resulted in formation of less extensive lipid droplets, mitochondria and ribosomes compared to pinealocyte ultrastructure of both control and ovariectomized rats. Extensiveness of rough endoplasmic reticulum in the pinealocytes of estradiol-administrated rats was similar to that in controls. Conclusions: The results confirm relationship between the pineal gland and gonads in the rat and it has been suggested that estradiol benzoate reverses the ultrastructural changes, which indicate increased cell activation, occurring in the pinealocytes after ovariectomy Daha fazlası Daha az

Effect of melatonin and n-acetylcysteine on hepatic injury in rat induced by methanol intoxication: A comparative study

Koksal M. | Kurcer Z. | Erdogan D. | Iraz M. | Tas M. | Eren M.A. | Aydogan T.

Review | 2012 | European Review for Medical and Pharmacological Sciences16 ( 4 ) , pp.437 - 444

Background: Methanol intoxication leads liver injury; in contrast melatonin and n-acetyl cysteine (NAC) are known to have protective effects on liver. Aim: We aimed to investigate the ultrastructural effects of melatonin and NAC on livers of methanol intoxicated rats and compare potential protective effects of melatonin and NAC on their liver ultrastructure. Materials and Methods: Fifty-six adult male Wistar rats were carried out and were randomized to eight groups that have seven rats each: Control groups (C 6h, C 24h), treated with intragastric (i.g.) 1.0 ml saline; Methanol groups (M 6h, M 24h), treated with a dose of 3 g/kg i.g. . . . methanol; Melatonin plus methanol groups (MEL+M 6h, MEL+M 24h), treated with dose of 10 mg/kg i.p melatonin immediately, following with a dose of 3 g/kg i.g. methanol; NAC plus methanol groups (NAC+M 6h, NAC+M 24h), treated with dose of 150 mg/kg, following with a dose of 3 g/kg i.g. methanol. 24 h group rats were given the same dose of melatonin and NAC 12 h after intoxication. Electron microscopy was used to evaluate histological changes in liver tissue at both 6th and 24th hour. Results: Histopathological damage was found to be higher in methanol-induced intoxicated rats compared with the controls. Extensive tubules of smooth endoplasmic reticulum, increased mitochondria, increased primary lysosomes and some marked openings of bile canaliculus were distinguished. Melatonin administration prevents liver injury especially in early hours and although not as effective as melatonin, NAC also prevents liver injury. Conclusions: Melatonin is much more efficient than NAC, as well as significantly greater hepatoprotective effect against the liver injury secondary to the methanol intoxication Daha fazlası Daha az

Detailed spectral profile analysis of penicillin-induced epileptiform activity in anesthetized rats

Canan S. | Ankarali S. | Marangoz C.

Article | 2008 | Epilepsy Research82 ( 1 ) , pp.7 - 14

Penicillin model is a widely used experimental model for epilepsy research. In the present study we aimed to portray a detailed spectral analysis of penicillin-induced epileptiform activity in comparison with basal brain activity in anesthetized Wistar rats. Male Wistar rats were anesthetized with i.p. urethane and connected to an electrocorticogram setup. After a short period of basal activity recording, epileptic focus was induced by injecting 400 IU/2 µl penicillin-G potassium into the left lateral ventricle while the cortical activity was continuously recorded. Basal activity, latent period and the penicillin-induced epileptifor . . .m activity periods were then analyzed using both conventional methods and spectral analysis. Spectral analyses were conducted by dividing the whole spectrum into different frequency bands including delta, theta (slow and fast), alpha-sigma, beta (1 and 2) and gamma (1 and 2) bands. Our results show that the most affected frequency bands were delta, theta, beta-2 and gamma-2 bands during the epileptiform activity and there were marked differences in terms of spectral densities between three investigated episodes (basal activity, latent period and epileptiform activity). Our results may help to analyze novel data obtained using similar experimental models and the simple analysis method described here can be used in similar studies to investigate the basic neuronal mechanism of this or other types of experimental epilepsies. © 2008 Elsevier B.V. All rights reserved Daha fazlası Daha az

The effects of oral administration of Aloe vera [barbadensis] on rat central nervous system: An experimental preliminary study

Kosif R. | Aktas R.G. | Oztekin A.

Article | 2008 | Neuroanatomy7 , pp.22 - 27

Aloe vera [barbadensis] (Av) is widely used for both commercial and therapeutic purposes. It has been used for an array of ailments since ancient times as a medicinal plant. There are more than 360 diffcrent species of Av. Its products have been used in health foods for medical and preservative purposes. The objective of this study was to investigate the effects of Av on the rat's central nervous system; since there are limited studies on that issue. Gel form of Av is used in the study. It is commercial, preserved but otherwise untreated form of Av. Female Wistar Albino rats were divided into three study groups. Tissue specimens fro . . .m cerebrum, cerebellum, hippocampus and ventricular area were processed for the microscopic examination. All sections from each group were stained with hematoxylin eosin and cresyl violet. Our results indicate that Av did not have any clear toxic effects on both neurons and glial cells of the central nervous system in different areas. Cytoplasmic features of the neurons, Nissle bodies, axonal hillock, and nuclei of neurons were the same after the treatment. However; the relationship between the Purkinje cells and the surrounding cerebellar tissue was decreased in the treated group. The other important finding was the change of ependymal cells at the ventricular zone: The number and the height of these cells were obviously increased. The single layered epithelium changed into the stratified epithelium in certain areas. It was also evident that microvilli and the cilia on the apical side of these cell increased dramatically. The capillaries in the region of choroid plexus were also dramatically increased. We believe that further studies related with these morphological changes will be helpful to understand the mechanism(s) of the similar transformation of the cells in different conditions. © Neuroanatomy Daha fazlası Daha az

Does intraabdominal use of Ankaferd Blood Stopper cause increased intraperitoneal adhesions? [Ankaferd Blood Stopper'in karin içi kullanim periton içi yapişklk oluşumunu arttrr m?]

Cömert M. | Karakaya K. | Barut F. | Karadeniz Çakmak G. | Uçan H.B. | Gültekin F.A. | Emre A.U.

Article | 2010 | Ulusal Travma ve Acil Cerrahi Dergisi16 ( 5 ) , pp.383 - 389

Background The aim of this study was to investigate whether intraabdominal Ankaferd Blood Stopper (ABS) causes increased intraabdominal adhesion formation and to determine any side effects of ABS in vivo. Methods The present experimental study was designed to examine the effects of Ankaferd solution on peritoneal adhesion formation in a rat model of cecal abrasion. Intraperitoneal adhesions were assessed macroscopically and histopathologically on the 10th postoperative day. The possible adverse affects of ABS on liver and lung tissues were analyzed histopathologically, and blood chemistry was also evaluated. Results Our study reveal . . .ed that ABS reduced intraperitoneal adhesion formation in an experimental rat model. The blood chemistry was not disturbed due to ABS administration. Intraperitoneal administration of ABS led to some minor changes in the lungs and serosal surfaces of the intestines, with minor architectural changes in the liver that were not considered as toxic. Further studies with various application doses and routes with more detailed cellular analysis are thus warranted to clarify the possible pleiotropic and adverse effects of this new agent away from hemostasis. Conclusion There was less intraperitoneal adhesion formation in the ABS group than in the control group and saline group. Intraperitoneal administration of ABS has no toxic effects on blood chemistry or the lungs, kidneys and the liver, but it has some minor adverse effects Daha fazlası Daha az

Rectal dexmedetomidine in rats: evaluation of sedative and mucosal effects

Hanci, Volkan | Gulle, Kanat | Karakaya, Kemal | Yurtlu, Serhan | Akpolat, Meryem | Yuce, Mehmet Fatih | Yuce, Fatma Zehra

Article | 2015 | REVISTA BRASILEIRA DE ANESTESIOLOGIA65 ( 1 ) , pp.1 - 6

Background and objectives: In this study, we investigated the anesthetic and mucosal effects of the rectal application of dexmedetomidine to rats. Methods: Male Wistar albino rats weighing 250-300 g were divided into four groups: Group S (n=8) was a sham group that served as a baseline for the normal basal values; Group C (n=8) consisted of rats that received the rectal application of saline alone; Group IPDex (n=8) included rats that received the intraperitoneal application of dexmedetomidine (100 mu g kg(-1)); and Group RecDex (n=8) included rats that received the rectal application of dexmedetomidine (100 mu g kg(-1)). For the re . . .ctal drug administration, we used 22 G intravenous cannulas with the stylets removed. We administered the drugs by advancing the cannula 1 cm into the rectum, and the rectal administration volume was 1 mL for all the rats. The latency and anesthesia time (min) were measured. Two hours after rectal administration, 75 mg kg(-1) ketamine was administered for intraperitoneal anesthesia in all the groups, followed by the removal of the rats' rectums to a distal distance of 3 cm via an abdominoperineal surgical procedure. We histopathologically examined and scored the rectums. Results: Anesthesia was achieved in all the rats in the Group RecDex following the administration of dexmedetomidine. The onset of anesthesia in the Group RecDex was significantly later and of a shorter duration than in the Group IPDEx ( Daha fazlası Daha az

Multi-organ injuries caused by lipopolysaccharide-induced periodontal inflammation in rats: Role of melatonin

Gulle K. | Akpolat M. | Kurcer Z. | Cengiz M.I. | Baba F. | Acikgoz S.

Article | 2014 | Journal of Periodontal Research49 ( 6 ) , pp.736 - 741

Background and Objective: Periodontitis, a chronic inflammatory disease caused by oral bacterial infection, is considered to be a risk factor for systemic diseases including diabetes mellitus, bacterial pneumonia, hyperlipidemia and atherosclerosis. The aim of this study was to evaluate the effectiveness of melatonin against periodontal inflammation-induced multiple organ injury in rats. Material and Methods: Eighteen female Wistar albino rats were randomly divided into three groups of six rats each: control; lipopolysaccharide (LPS); and LPS + melatonin. During the experimental period (10 d) all rats in the LPS and LPS + melatonin . . .groups were given 10 µL of LPS (from a 10 mg/mL standard solution of LPS dissolved in saline) on days 1, 3 and 5. The rats in the LPS + melatonin group were given 50 mg/kg of melatonin, daily for 10 d, starting on day 1 after the administration of LPS. All rats were killed at the end of the experimental period. Liver, kidney and lung tissues were removed for investigation by light microscopy. Results: The levels of serum aspartate aminotransferase (AST), alanine transaminase (ALT) and blood urea nitrogen (BUN) were significantly increased in the LPS group compared with the LPS + melatonin group (p Daha fazlası Daha az

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