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Effects of melatonin on testis histology, oxidative stress and spermatogenesis after experimental testis ischemia-reperfusion in rats

Koksal M. | Oguz E. | Baba F. | Eren M.A. | Ciftci H. | Demir M.E. | Kurcer Z.

Review | 2012 | European Review for Medical and Pharmacological Sciences16 ( 5 ) , pp.582 - 588

Background: Testicular torsion due to oxidative stress results in infertility and testicular damage which can be preventable an important health problem worldwide. Aim: The purpose of the present study was to investigate the changes of malondialdehyde (MDA), total antioxidant status (TAS), total oxidant status (TOS) levels; histopathological alterations; morphology, concentration and motilities of the sperm in post ischemic reperfused (I/R) testis tissue. Materials and Methods: Forty adult male Wistar rats were carried out and were randomized to five groups; (1) Control group, (2) Ipsilateral left testis ischemia, (3) Melatonin plus . . . ipsilateral left testis ischemia, (4) Contralateral right testis ischemia, 5. Melatonin plus contralateral right testis ischemia. After 1 h ischemia and 24 h perfusion; MDA, TAS and TOS levels were measured, histopathological alterations were determined using by Johnsen's score (JS) and sperm morphology, concentration, motility were examined. Results: MDA, TAS and TOS levels of the testis tissue did not change in all groups (p < 0.05 for all). JS was decreased in I/R group and melatonin treatment reversed histopathological changes and increased JS both in ipsilateral and contralateral testis. Abnormal sperm rate significantly increased in I/R group and melatonin administration changed abnormal sperm rate to normal. Conclusions: As a result, the present study demonstrated that testicular damage occurs following I/R without an increase of MDA, TAS and TOS levels. Our results also suggested that melatonin is a potent antioxidant agent in preventing testicular I/R injury, as shown by increased JS and changed abnormal sperm rate Daha fazlası Daha az

The association of FOXO3A gene polymorphisms with serum FOXO3A levels and oxidative stress markers in vitiligo patients

Ozel Turkcu U. | Solak Tekin N. | Gokdogan Edgunlu T. | Karakas Celik S. | Oner S.

Article | 2014 | Gene536 ( 1 ) , pp.129 - 134

Vitiligo is an acquired epidermal pigment loss of the skin. Oxidative stress is one of the major theories in the pathophysiology of vitiligo. FOXO3A is the forkhead members of the class O (FOXO) transcription factors, and plays an important role in cell cycle regulation, apoptosis, oxidative stress, and DNA repair. The aim of our study was to investigate FOXO3A gene polymorphisms and FOXO3A protein levels, activities of superoxide dismutase (SOD) and catalase antioxidant enzymes in vitiligo patients and healthy controls. Moreover, the level of plasma advanced oxidation protein products (AOPP) in subjects was evaluated to understand . . .the possible role of protein oxidation in disease etiology. Study groups included 82 vitiligo patients and 81 unrelated healthy controls. FOXO3A polymorphisms were determined using polymerase chain reaction-restriction fragment length polymorphism method. FOXO3A levels and catalase activity were measured by ELISA whereas AOPP levels and SOD activity was measured by spectrophotometric analysis. We found a significant relationship between rs4946936 polymorphism of FOXO3A gene and vitiligo/active vitiligo patients (p = 0.017; p = 0.019 respectively), but not for rs2253310 (p > 0.05). SOD activity and AOPP levels of vitiligo patient were increased compared with control group, whereas FOXO3A levels and catalase enzyme activity of vitiligo patient were decreased compared with control group (p < 0.05). Our study indicates that rs4946936 of FOXO3A gene may associate susceptibility of vitiligo, especially active vitiligo. Moreover, our results confirm that oxidative stress may play a role in the pathophysiology of vitiligo. Further studies with larger samples are required to elucidate the role of FOXO3A in vitiligo. © 2013 Elsevier B.V Daha fazlası Daha az

The presence of oxidized low-density lipoprotein and inducible nitric oxide synthase expression in renal damage after intestinal ischemia reperfusion

Yurdakan G. | Tekin I.O. | Comert M. | Acikgoz S. | Sipahi E.Y.

Article | 2012 | Kaohsiung Journal of Medical Sciences28 ( 1 ) , pp.16 - 22

Intestinal ischemia/reperfusion (I/R) is a complex phenomenon that causes destruction of both local and remote tissues. The objective of this study was to investigate the possible participation of oxidized low-density lipoproteins (oxLDLs) and inducible nitric oxide synthase (iNOS) expression in renal tissue damage after intestinal I/R. The superior mesenteric artery was blocked for 30 minutes, followed by 24 hours of reperfusion. At the end of the reperfusion period, renal tissues were removed; the presence of oxLDL, superoxide dismutase enzyme activity, malondialdehyde levels, and iNOS expression were evaluated. I/R resulted in po . . .sitive oxLDL staining in renal tissue. Compared with control rats, tissue from the I/R group showed significantly higher malondialdehyde levels and lower superoxide dismutase enzyme activity. Strong and diffuse iNOS expression was present in the I/R group. Our findings support the hypothesis that I/R of intestinal tissue results in oxidative and nitrosative stress and enhances lipid peroxidation in the end organ. These data show that oxLDL accumulates in rat renal tissue after intestinal I/R. Antioxidant strategies may provide organ protection in patients with reperfusion injury, at least by affecting interactions with free radicals, nitric oxide, and oxLDL. This study demonstrates for the first time that oxLDL may play a role in renal tissue damage after intestinal I/R. Antioxidant strategies may be beneficial for protection from reperfusion injury. © 2011, Elsevier Taiwan LLC. All rights reserved Daha fazlası Daha az

Can ursolic acid be beneficial against diabetes in rats?

Bacanli M. | Aydin S. | Bucurgat U.U. | Başaran N. | Anlar H.G. | Çal T. | Ari N.

Article | 2018 | Turkish Journal of Biochemistry43 ( 5 ) , pp.520 - 529

Objective: Diabetes mellitus, a heteregenous metabolic and chronic disease, is a growing health problem especially in developing countries. It is claimed that diabetes associated with increased formation of free radicals and decrease in antioxidant potential and also alterations in lipid profile and enzyme levels. Ursolic acid is commonly used in traditional Chinese medicine due to its beneficial effects. The aim of this study was to investigate the effects of ursolic acid on streptozotocin-induced diabetes in Wistar albino rats. Methods: DNA damage was evaluated in the blood and liver cells of rats by alkaline comet assay. The acti . . .vities of antioxidant enzymes, oxidative stress parameters, biochemical parameters, hepatic enzyme levels and lipid profile parameters were also evaluated. Results: The results of this study demonstrate that diabetes caused genotoxic damage, changes in hepatic enzyme and lipid profile, biochemical and antioxidant enzyme activities and oxidative stress parameters in rats. Ursolic acid was found to be protective against diabetes induced effects in blood and liver samples of rats. Conclusions: According to our results, it seems that ursolic acid may be beneficial against diabetes and its adverse effects in rats. © 2018 Turkish Biochemistry Society. All rights reserved Daha fazlası Daha az

Effects of Nigella sativa on oxidative stress and ß-cell damage in streptozotocin-induced diabetic rats

Kanter M. | Coskun O. | Korkmaz A. | Oter S.

Article | 2004 | Anatomical Record - Part A Discoveries in Molecular, Cellular, and Evolutionary Biology279 ( 1 ) , pp.685 - 691

The aim of the present study was to evaluate the possible protective effects of Nigella sativa L. (NS) against ß-cell damage from streptozotocin (STZ)-induced diabetes in rats. STZ was injected intraperitoneally at a single dose of 50 mg/kg to induce diabetes. NS (0.2 ml/kg/day, i.p.) was injected for 3 days prior to STZ administration, and these injections were continued throughout the 4-week study. Oxidative stress is believed to play a role in the pathogenesis of diabetes mellitus (DM). To assess changes in the cellular antioxidant defense system, we measured the activities of antioxidant enzymes (such as glutathione peroxidase ( . . .GSHPx), superoxide dismutase (SOD), and catalase (CAT)) in pancreatic homogenates. We also measured serum nitric oxide (NO) and erythrocyte and pancreatic tissue malondialdehyde (MDA) levels, a marker of lipid peroxidation, to determine whether there is an imbalance between oxidant and antioxidant status. Pancreatic ß-cells were examined by immunohistochemical methods. STZ induced a significant increase in lipid peroxidation and serum NO concentrations, and decreased antioxidant enzyme activity. NS treatment has been shown to provide a protective effect by decreasing lipid peroxidation and serum NO, and increasing antioxidant enzyme activity. Islet cell degeneration and weak insulin immunohistochemical staining was observed in rats with STZ-induced diabetes. Increased intensity of staining for insulin, and preservation of ß-cell numbers were apparent in the NS-treated diabetic rats. These findings suggest that NS treatment exerts a therapeutic protective effect in diabetes by decreasing oxidative stress and preserving pancreatic ß-cell integrity. Consequently, NS may be clinically useful for protecting ß-cells against oxidative stress. © 2004 Wiley-Liss, Inc Daha fazlası Daha az

Free oxygen radicals associated with growth in coeliac disease

Ozcetin M. | Katar M. | Yilmaz R. | Karaaslan E. | Ozugurlu F.

Article | 2011 | HealthMED5 ( 5 ) , pp.1008 - 1013

Introduction: Coeliac Disease (CD) is an immune- mediated chronic inflammatory disease of upper small intestine in genetically permanent gluten-sensitive individuals. Oxidative stress was reported to play an important role in the pathogenesis of coeliac disease. The aim of this study was to investigate the frequency of the polimorphisms in the structures of the enzymes SOD and GSHPx with changing levels depending on increased oxidative stress and whether there is an association with the mutations DQA1*0501, DQB1*0201, DRB1*04 reported frequently in coeliac disease. Methods: This study has investigated SOD and GSH-PX polymorphisms an . . .d the frequently reported mutations DQA1*0501, DQB1*0201 and DRB1*04 in the patients with CD. Height and weight measurements of the patients were obtained to evaluate their growth and development, also correlation between polymorphisms SOD and GSH-PX and concerned mutations were investigated. Results: This study involved total 56 cases, 35 female (62.5%) and 21 male (37.5%), with a mean age 6.66 ± 4.18 years. Polymorphisms SOD and GSH-PX were found in homozygote, heterozygote and wild-type patients. At least one of the mutations DQA1*0501, DQB1*0201 and DRB1*04 were found in 41 patients. Conclusion: Although etiology of coeliac disease is not entirely clear, many mechanisms have been suggested. It may be observed that the retardation of growth and development in the patients with coeliac disease may be associated with oxidative stress and decreased antioxidant capacity Daha fazlası Daha az

Overektomize dişi sıçanlarda beyin oksidatif stres, bdnf ve inflamatuar cevap düzeyleri ile nörodavranışsal değişiklikler üzerine anjiyotensin II İnhibisyonunun etkisi

Erdem, Salih

Master Thesis | 2020 | Zonguldak Bülent Ecevit Üniversitesi, Sağlık Bilimleri Enstitüsü, Fizyoloji Anabilim Dalı

Menopoz overlerden hormon sekresyonunun azalması veya durması ile karakterize doğal fizyolojik bir süreçtir. Depresyon ve anksiyete menopozda en sık karşılaşılan semptomlardandır. Yapılan çalışmalar depresyon patogenezinde renin-anjiyotensin-aldesteron sisteminin, oksidatif stres hasarının, nörotrofik faktörlerin ve inflamatuar sitokinlerin kritik bir rol oynadığını göstermiştir. Biz bu çalışmada anjiyotensin II tip I reseptör blokerinin menopoz kaynaklı depresyon ve anksiyete benzeri nörodavranışlar üzerine etkisini araştırmayı ve olası etki mekanizmalarını beyin dokusunda nod-benzeri reseptör protein 3, interlökin-1beta, beyin kay . . .naklı nörotrofik faktör ve beyin oksidatif stres düzeylerinin belirlenmesi ile açıklamayı amaçladık. Çalışmamızda 32 adet Wistar albino türü dişi sıçan kullanıldı ve rastgele olarak 4 grup oluşturuldu. Deneysel menopoz modeli olan overektomi grup 3 ve grup 4’deki deneklere uygulandı. Grup 4 hayvanları ondört gün süreyle 40mg/kg/gün dozda valsartan ile tedavi edildi. Deney protokolünün sonunda depresyon ve anksiyete benzeri davranışları değerlendirmek için zorlu yüzme testi ve açık alan testi yapıldı. Sıçanlardan alınan hipokampüs ve prefrontal korteks dokularında oksidatif stres, NLRP3, IL-1β, BDNF ve CREB analizleri yapıldı. Davranış testleri sonucunda depresyon ve anksiyete benzeri davranışlar overektomize sıçanlarda artış gösterirken valsartan tedavisi bu davranışları azalttığı görüldü. Overektomize sıçanların hipokampüsünde oksidatif stres, NLRP3 ve IL-1β konsantrasyonu artarken BDNF konsantrasyonu azaldı. Valsartan tedavisi oksidatif stres parametrelerini ve BDNF seviyeleri üzerine iyileştirici bir etki gösterirken, overektomi ile artan NLRP3 ve IL- 1β seviyelerini etkilemediği saptandı. Sonuç olarak anjiyotensin II tip I reseptör blokeri depresyon ve anksiyete benzeri davranış üzerinde iyileştirici etkiye sahiptir. anjiyotensin II tip I reseptör blokerinin bu terapötik etki mekanizmasında hipokampüsde oksidatif stres düzeylerinde azalma ve BDNF yapımındaki artışın rol oynadığı görülmektedir. Menopause is a natural physiological process characterized by decreased or stopped which hormone secreted from ovaries. Depression and anxiety are the most common symptoms in menopause. Studies have shown that renin-angiotensin-aldesterone system, oxidative stress damage, neurotrophic factors and inflammatory cytokines play a critical role in the pathogenesis of depression. In this study, we aimed to explain the effect of angiotensin II type I receptor blocker on menopaus-induced depression and anxiety-like neurobehavior and to possible mechanisms of action by determining the levels of nod-like receptor protein 3, interleukin-1beta, brain-derived neurotrophic factor and brain oxidative stress in brain tissue. In this study, 32 female Wistar albino rats were used and 4 groups were randomly formed. The experimental menopause model, overectomy, was applied to the subjects in group 3 and 4. Group 4 animals were treated with valsartan at a dose of 40 mg / kg / day for fourteen days. At the end of the experimental protocol, a forced swimming test and open field test were performed to assess depression and anxiety-like behaviors. Oxidative stress, NLRP3, IL-1β, BDNF and CREB were analyzed in hippocampus and prefrontal cortex tissues from rats. Behavioral tests showed that depression and anxiety-like behaviors increased in ovariectomized rats, whereas valsartan treatment decreased these behaviors. In the hippocampus of ovariectomized rats, oxidative stress, NLRP3 and IL-1β concentration increased while BDNF concentration decreased. While valsartan treatment had an improving effect on oxidative stress parameters and BDNF levels, it did not affect increased NLRP3 and IL-1β levels with OVX. As a result angiotensin II type I receptor blocker has a curative effect on depression and anxiety-like behaviors. This therapeutic action of mechanism of angiotensin II type I receptor blocker appears to play a role in reduction of oxidative stress level and increase in BDNF production in the hippocampus Daha fazlası Daha az

Preventive role of Pycnogenol ® against the hyperglycemia-induced oxidative stress and DNA damage in diabetic rats

Aydın S. | Bacanlı M. | Anlar H.G. | Çal T. | Arı N. | Ündeğer Bucurgat Ü. | Başaran A.A.

Article | 2019 | Food and Chemical Toxicology124 , pp.54 - 63

Diabetes mellitus, a complex progressive metabolic disorder, leads to some oxidative stress related complications. Pycnogenol ® (PYC), a plant extract obtained from Pinus pinaster, has been suggested to be effective in many diseases including diabetes, cancer, inflammatory and immune system disorders. The mechanisms underlying the effects of PYC in diabetes need to be elucidated. The aim of this study was to determine the effects of PYC treatment (50 mg/kg/day, orally, for 28 days) on the DNA damage and biochemical changes in the blood, liver, and kidney tissues of experimental diabetic rats. Changes in the activities of catalase, s . . .uperoxide dismutase, glutathione peroxidase, glutathione reductase, and glutathione-S-transferase enzymes, and the levels of 8-hydroxy-2'-deoxyguanosine, total glutathione, malondialdehyde, insulin, total bilirubin, alanine aminotransferase, aspartate aminotransferase, gamma-glutamyl transferase, high density lipoprotein, low density lipoprotein, total cholesterol, and triglyceride were evaluated. DNA damage was also determined in the whole blood cells and the liver and renal tissue cells using the alkaline comet assay. PYC treatment significantly ameliorated the oxidative stress, lipid profile, and liver function parameters as well as DNA damage in the hyperglycemic rats. The results show that PYC treatment might improve the hyperglycemia-induced biochemical and physiological changes in diabetes. © 201 Daha fazlası Daha az

Examination of lung toxicity, oxidant/antioxidant status and effect of erdosteine in rats kept in coal mine ambience

Armutcu F. | Gun B.D. | Altin R. | Gurel A.

Article | 2007 | Environmental Toxicology and Pharmacology24 ( 2 ) , pp.106 - 113

Occupational exposure to coal dust causes pneumoconiosis and other diseases. Reactive oxygen species (ROS) have been implicated in the pathogenesis of coal dust-induced lung toxicity. In this experimental study, we investigated the oxidant/antioxidant status, nitric oxide (NO) and hydroxyproline (HP) levels in lungs and blood of rats exposed to coal dust in mine ambience. In addition, we also investigated the attenuating effects of erdosteine. At the end of the experiment processes, tissue levels of HP, malondialdehyde (MDA) and NO, as well as the activities of superoxide dismutase, glutathione peroxidase, catalase, xanthine oxidase . . . (XO), myeloperoxidase (MPO) and proinflammatory cytokines (IL-6 and TNF-?) were evaluated in the lung tissues, plasma samples or erythrocytes of rats. Exposure to coal dust resulted in a significant increase in the oxidant parameters (MDA, NO levels, and XO activity) and HP levels, as compared to the controls. A decrease in activities of antioxidant enzymes, and an increase in MPO activity were found in the study group, compared to the controls. Increased NO levels of lung were found in the study groups, that were significantly reduced by erdosteine. Our studies provide evidence that supports the hypothesis for ROS mediated coal workers' pneumoconiosis. Erdosteine may be beneficial in the coal dust-induced lung toxicity via antioxidant and free radical scavenger properties. © 2007 Elsevier B.V. All rights reserved Daha fazlası Daha az

The influence of dietary alpha-solanine on the waxmoth galleria mellonellal

Büyükgüzel, Ender | Büyükgüzel, Kemal | Erdem, Meltem | Adamski, Zbigniew | Adamski, Zbigniew | Marciniak, Pawel | Ziemnicki, Kazimierz

Article | 2013 | Archives of Insect Biochemistry and Physiology83 ( 1 ) , pp.15 - 24

Plant allelochemicals are nonnutritional chemicals that interfere with the biology of herbivores. We posed the hypothesis that ingestion of a glycoalkaloid allelochemical, ?-solanine, impairs biological parameters of greater wax moths Galleria mellonella. To test this idea, we reared wax moths on artificial diets with 0.015, 0.15, or 1.5 mg/100 g diet of ?-solanine. Addition of ?-solanine to the diet affected survival of seventh-instar larvae, pupae, and adults; and female fecundity and fertility. The diet containing the highest ?-solanine concentration led to decreased survivorship, fecundity, and fertility. The diets supplemented . . .with ?-solanine led to increased malondialdehyde and protein carbonyl contents in midgut and fat body and the effect was dose-dependent. Dietary ?-solanine led to increased midgut glutathione S-transferase activity and to decreased fat body glutathione S-transferase activitiy. We infer from these findings that ?-solanine influences life history parameters and antioxidative enzyme activities in the midgut and fat body of G. mellonella. © 2013 Wiley Periodicals, Inc Daha fazlası Daha az

Preventive role of Pycnogenol (R) against the hyperglycemia-induced oxidative stress and DNA damage in diabetic rats

Aydin, Sevtap | Bacanli, Merve | Anlar, Hatice Gul | Cal, Tugbagul | Ari, Nuray | Bucurgat, Ulku Undeger | Basaran, Arif Ahmet

Article | 2019 | FOOD AND CHEMICAL TOXICOLOGY124 , pp.54 - 63

Diabetes mellitus, a complex progressive metabolic disorder, leads to some oxidative stress related complications. Pycnogenol (R) (PYC), a plant extract obtained from Pinus pinaster, has been suggested to be effective in many diseases including diabetes, cancer, inflammatory and immune system disorders. The mechanisms underlying the effects of PYC in diabetes need to be elucidated. The aim of this study was to determine the effects of PYC treatment (50 mg/kg/day, orally, for 28 days) on the DNA damage and biochemical changes in the blood, liver, and kidney tissues of experimental diabetic rats. Changes in the activities of catalase, . . . superoxide dismutase, glutathione peroxidase, glutathione reductase, and glutathione-S-transferase enzymes, and the levels of 8-hydroxy-2'-deoxyguanosine, total glutathione, malondialdehyde, insulin, total bilirubin, alanine aminotransferase, aspartate aminotransferase, gamma-glutamyl transferase, high density lipoprotein, low density lipoprotein, total cholesterol, and triglyceride were evaluated. DNA damage was also determined in the whole blood cells and the liver and renal tissue cells using the alkaline comet assay. PYC treatment significantly ameliorated the oxidative stress, lipid profile, and liver function parameters as well as DNA damage in the hyperglycemic rats. The results show that PYC treatment might improve the hyperglycemia-induced biochemical and physiological changes in diabetes Daha fazlası Daha az

Evaluation of preventive effect of shilajit on radiation-induced apoptosis on ovaries

Kececi M. | Akpolat M. | Gulle K. | Gencer E. | Sahbaz A.

Article | 2016 | Archives of Gynecology and Obstetrics293 ( 6 ) , pp.1255 - 1262

Purpose: Cancer is the second leading cause of death in children in developed countries and most of childhood malignancies can be treated with chemo-radiotherapy. Although radiation therapy is a successful treatment modality in cancer patients, it has various adverse effects. Especially the gonads are very sensitive and prone to radiation-related damage. Radiation impairs the ovaries by triggering apoptosis of follicular cells and chromosomal damage and oxidative stress. Shilajit, a traditional medicinal agent in India, Russia, and other parts of the world, contains various antioxidant agents and has ovogenic effects. To evaluate th . . .e ability of shilajit to prevent radiation-induced ovarian damage. Methods: Forty Wistar albino female rats were divided into four groups as: Control group, shilajit group, radiation only group, and radiation + shilajit group. Four days after radiation exposure, the rats were sacrificed and the ovaries were removed and evaluated immuno-histopathologically. Results: There was a statistically significant difference in follicle counts (primordial, primary, preantral, antral, and atretic follicles) between the groups (p Daha fazlası Daha az

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