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Erythropoietin stimulates wound healing and angiogenesis in mice

Sayan, Hale | Özaçmak, Veysel Haktan | Güven, Aysel | Aktaş, R. Gülhan | Özaçmak, I. Diler

Article | 2006 | Journal of Investigative Surgery19 ( 3 ) , pp.163 - 173

Erythropoietin exerts hematopoietic effects by stimulating proliferation of early erythroid precursors. Nonhematopoietic effects of erythropoietin have also been shown. It may act as a new angiogenic factor in wound healing. This study aimed to investigate the effect of systemic administration of recombinant human erythropoietin on wound healing in mice. Dorsal incisional wounds were performed in mice, which were then divided into two groups; a group treated for 7 days with recombinant human erythropoietin, and a control group. Sacrificing animals on day 7, the wound tissues were collected for analysis of wound breaking strength, ma . . .londialdehyde, a marker of lipid peroxidation, hydroxyproline, an index of reparative collagen deposition, reduced glutathione levels, and for histological evaluation. The immunohistochemical determination of vascular endothelial growth factor (VEGF) which is believed to be the most prevalent angiogenic factor throughout the skin repair process, was also studied. The treatment significantly increased wound breaking strength by decreasing malondialdehyde and increasing hydroxyproline levels on day 7 after wounding. No statistically meaningful change was observed in reduced glutathione content. VEGF was immunostained significantly more on wound tissue of treated animals compared to the control group. Recombinant human erythropoietin treatment may be effective in wound healing due to inhibition of lipid peroxidation, deposition of collagen, and VEGF expression in wound area. Copyright © Taylor & Francis Group, LLC Daha fazlası Daha az

N-acetylcycsteine attenuates the deleterious effects of radiation therapy on incisional wound healing in rats

Tascilar O. | Çakmak G.K. | Emre A.U. | Bakkal, Bekir Hakan | Kandemir N. | Turkcu U.O. | Demir E.O.

Article | 2014 | Hippokratia18 ( 1 ) , pp.17 - 23

Background: During preoperative radiotherapy, effective doses of ionizing radiation occasionally cause wound complications after subsequent surgery. This study was designed to determine the effects of intraperitoneally or orally administered N-acetylcysteine (NAC) on anastomotic healing of irradiated rats. Material & Methods: Forty Wistar albino rats were randomized into four groups containing 10 rats each. A 3 cm long surgical full-thickness midline laparotomy was performed to all groups (Groups 1-4). Group 1 was designed as a control group without radiation therapy and NAC treatment. Groups 2, 3 and 4 received a single abdominal d . . .ose of 10 Gy irradiation before laparotomy and groups 3 and 4 received oral and intraperitoneal NAC, respectively. Results: Group comparisons demonstrated that breaking strength was significantly higher in NAC treated rats. A statistically significant difference was determined in terms of superoxide dismutase (SOD), malondealdehyde (MDA) and glutation (GSH) values between groups ( Daha fazlası Daha az

Vitamin E protects against acetone-induced oxidative stress in rat red blood cells

Armutçu, Feraf | Coşkun, Ömer Faruk | Gürel, Aykut | Şahin, S. | Kanter, Marek | Cihan, Ali | Numanoğlu, Kemal Varın

Article | 2005 | Cell Biology and Toxicology21 ( 1 ) , pp.53 - 60

Acetone may induce oxidative stress leading to disturbance of the biochemical and physiological functions of red blood cells (RBCs) thereby affecting membrane integrity. Vitamin E (vit E) is believed to function as an antioxidant in vivo protecting membranes from lipid peroxidation. The aim of the present study was the evaluation of possible protective effects of vit E treatment against acetone-induced oxidative stress in rat RBCs. Thirty healthy male Wistar albino rats, weighing 200-230 g and averaging 12 weeks old were randomly allotted into one of three experimental groups: Control (A), acetone-treated (B) and acetone + vit E-tre . . .ated groups (C), each containing ten animals. Group A received only drinking water. Acetone, 5% (v/v), was given with drinking water to B and C groups. In addition, C group received vit E dose of 200 mg/kg/day i.m. The experiment continued for 10 days. At the end of the 10th day, the blood samples were obtained for biochemical and morphological investigation. Acetone treatment resulted in RBC membrane destruction and hemolysis, increased thiobarbituric acid reactive substance (TEARS) levels in plasma and RBC, and decreased RBC vit E levels. Vit E treatment decreased elevated TEARS levels in plasma and RBC and also increased reduced RBC vit E levels, and prevented RBC membrane destruction and hemolysis. In conclusion, vit E treatment appears to be beneficial in preventing acetone-induced oxidative RBC damage, and therefore, it can improve RBC rheology. © 2005 Springer Daha fazlası Daha az

Effect of boric acid on antioxidant enzyme activity, lipid peroxidation, and ultrastructure of midgut and fat body of Galleria mellonella

Büyükgüzel, Ender | Büyükgüzel, Kemal | Snela, Milena | Erdem, Meltem | Radtke, Katarzyna | Ziemnicki, Kazimierz | Adamski, Zbigniew

Article | 2013 | Cell Biology and Toxicology29 ( 2 ) , pp.117 - 129

Boric acid is widely used as an insecticide, acaricide, herbicide, and fungicide and also during various industrial processings. Hence, numerous populations are subjects to this toxic compound. Its action on animals is still not fully known and understood. We examined the effect of boric acid on larvae of greater wax moth (Galleria mellonella). The chemical appeared to be toxic for larvae, usually in a concentration-dependent manner. Exposed groups revealed increased lipid peroxidation and altered activity of catalase, superoxide dismutase, glutathione S-transferase, and glutathione peroxidase. We also observed changes of ultrastruc . . .ture, which were in tune with biochemical assays. We suggest that boric acid has a broad mode of action, which may affect exposed larvae, and even if sublethal, they may lead to disturbances within exposed populations. © 2013 The Author(s) Daha fazlası Daha az

Erdosteine improves oxidative damage in a rat model of renal ischemia-reperfusion injury

Gürel, Ahmet | Armutcu, Ferah | Cihan, Alper | Numanoğlu, Kemal Varın | Unalacak, Murat

Article | 2004 | European Surgical Research36 ( 4 ) , pp.206 - 209

The aim of the present study was to determine the effects of erdosteine, a new antioxidant and anti-inflammatory agent, on lipid peroxidation, neutrophil infiltration, and antioxidant enzyme activities in a rat model of renal ischemia-reperfusion (I/R) injury. Twenty-eight rats were divided into three groups: sham operation, I/R, and I/R plus erdosteine groups. After the experimental procedure, rats were sacrificed and kidneys were removed and prepared for malondialdehyde (MDA) levels, myeloperoxidase (MPO), xanthine oxidase (XO), catalase (CAT) and superoxide dismutase (SOD) activities. MDA level, MPO and XO activities were signifi . . .cantly increased in the I/R group. On the other hand, SOD and CAT activities were found to be decreased in the I/R group compared to the sham group. Pretreatment with erdosteine significantly diminished tissue MDA level, MPO and XO activities. Our data support a role for erdosteine in attenuation in renal damage after I/R injury of the kidney, in part at least by inhibition of neutrophil sequestration and XO activity. Copyright © 2004 S. Karger AG, Basel Daha fazlası Daha az

Lipid peroxidation and homocysteine levels in Behçet's disease

Mungan A.G. | Can M. | Açikgöz S. | Eştürk E. | Altinyazar C.

Article | 2006 | Clinical Chemistry and Laboratory Medicine44 ( 9 ) , pp.1115 - 1118

Background: The aim of this study was to investigate serum paraoxonase (PON1) activity in relation to homocysteine, malondialdehyde (MDA) and lipid parameters in active and inactive Behçet's disease (BD). Methods: A total of 46 consecutive BD patients and 25 healthy control subjects were included in the present study. Results: Serum PON1 activity in both active and inactive BD was significantly lower compared with healthy subjects (p

Neuroprotective effects of Nigella sativa on experimental spinal cord injury in rats

Kanter M. | Coskun O. | Kalayci M. | Buyukbas S. | Cagavi F.

Article | 2006 | Human and Experimental Toxicology25 ( 3 ) , pp.127 - 133

The aim of this study was to investigate the possible beneficial effects of Nigella sativa (NS) in comparison to methylprednisolone on experimental spinal cord injury (SCI) in rats. SCI was performed by placing an aneurysm clip extradurally at the level of T11-12. Rats were neurologically tested over 24 h after trauma and spinal cord tissue samples were harvested for both biochemical and histopathological evaluation. The neurological scores of rats were not found to be different in SCI groups. SCI significantly increased the spinal cord tissue malondialdehyde (MDA) and protein carbonyl (PC) levels, however SCI decreased superoxide d . . .ismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) enzyme activities compared to the control. Methylprednisolone and NS treatment decreased tissue MDA and PC levels and prevented inhibition of SOD, GSH-Px and CAT enzymes in the tissues. The most significant results were obtained when NS was given. In SCI and placebo groups, the neurons of spinal cord tissue became extensively dark and degenerated with picnotic nuclei. The morphology of neurons in methylprednisolone and NS-treated groups were well protected, however, not as well as the neurons of the control group. The number of neurons in the spinal cord tissue of the SCI and placebo groups was significantly less than the control, laminectomy, methylprednisolone and NS-treated groups. In conclusion, NS treatment might be beneficial in spinal cord tissue damage, and therefore shows potential for clinical implications. © 2006 Edward Arnold (Publishers) Ltd Daha fazlası Daha az

Excessive dietary fructose is responsible for lipid peroxidation and steatosis in the rat liver tissues

Armutçu F. | Kanter M. | Gürel A. | Unalacak M.

Article | 2007 | Turkiye Klinikleri Journal of Medical Sciences27 ( 2 ) , pp.164 - 169

Objective: Drinking large amounts of carbonated beverage is associated with increased risk for obesity and nonalcoholic steatohepatitis. Fructose is found in many fruits, vegetables, honey and in soft drinks, such as high fructose corn syrup. Excessive fructose consumption can adversely affect liver metabolism and increase lipid peroxidation, and this plays a role in the pathology associated with fructose feeding. Material and Methods: The present study reports the effects of high-fructose diet (10% and 20% in the drinking water) on the liver lipid peroxidation of rats. At the end of the experiment, biochemical evaluation of plasma . . .and histopathological evaluation of liver tissue were performed. Results: Enhanced thiobarbituric acid-reactant substance levels and abnormal lipid changes were observed in high-fructose-fed rats. In 10% fructose-fed group, the most consistent findings in the histologic sections of liver tissues were the hepatocellular degenerative changes, minimal macrovesicular and microvesicular steatosis in zone 1. In the 20% fructose-fed group, the trabecular liver structure was more seriously affected than the 10% fructose-fed group. High fructose-induced degenerative changes were evident in numerous hepatocytes of zone 1; the cells were enlarged and had a light and foamy cytoplasm filled with vacuoles. Conclusion: Our study suggests that fructose feeding is associated with lipid peroxidation and steatosis in liver and the dose of fructose is associated with the degree of tissue damage. Copyright © 2007 by Türkiye Klinikleri Daha fazlası Daha az

Inhibitory effect of caffeic acid phenethyl ester on bleomycine-induced lung fibrosis in rats

Özyurt H. | Sögüt S. | Yildirim Z. | Kart L. | Iraz M. | Armutçu F. | Temel I.

Conference Object | 2004 | Clinica Chimica Acta339 ( 01.Feb ) , pp.65 - 75

Background: Pulmonary fibrosis (PF) induced by anticancerogenic bleomycin (BLM) is one of the more common side effects encountered during cancer treatment. It has been suggested in the last decades that the main responsible agent in PF is reactive oxygen species which were generated also in normal physiological conditions in the human body. In this experimental study, we investigated the preventive or attenuating effects of caffeic acid phenethyl ester (CAPE) that has been demonstrated to have anti-inflammatory, cytocytatic, anticancerogenic, antiprolipherative and antioxidant effects on BLM-induced PF. Methods: Thirty-six Sprague-D . . .awley rats were divided randomly into four groups as sham operation, BLM, BLM+vitamin E (vit E), and BLM+CAPE groups. BLM (7.5 mg/kg, single dose) was applied intratracheally, and CAPE and vit E intraperitoneally in the appropriate groups. At the end of the fibrosis processes, lung tissues were removed and the levels of tissues hydroxyproline (OH-proline), malondialdehyde (MDA) and NO as well as the activities of superoxide dismutase (SOD), catalase (CAT) and myeloperoxidase (MPO) were determined. Also, the weights of the rats were recorded at 7th and 14th days of the experiments. Results: BLM application to the rats resulted in a significant increase in the OH-proline level as compared to the controls. Administration of CAPE and vit E led to the remarkable reduction of total lung OH-proline levels compared to the rats treated with BLM alone ( Daha fazlası Daha az

Potential role of dietary ?-3 essential fatty acids on some oxidant/antioxidant parameters in rats' corpus striatum

Sarsilmaz M. | Songur A. | Özyurt H. | Kuş I. | Özen O.A. | Özyurt B. | Sögüt S.

Conference Object | 2003 | Prostaglandins Leukotrienes and Essential Fatty Acids69 ( 4 ) , pp.253 - 259

Omega-3 (?-3) is an essential fatty acid (EFA) found in large amounts in fish oil. It contains eicosapentaenoic acid and docosahexaenoic acid (DHA). DHA is one of the building structures of membrane phospholipids of brain and necessary for continuity of neuronal functions. Evidences support the hypothesis that schizophrenia may be the result of increased reactive oxygen species mediated neuronal injury. Recent reports also suggest the protective effect of ?-3 EFA against neuropsychiatric disorders including schizophrenia. This study proposed to assess the changes in antioxidant enzyme and oxidant parameters in the corpus striatum (C . . .S) of rats fed with ?-3 EFA diet (0.4 g/kg/day) for 30 days. Eight control rats and nine rats fed with ?-3 were decapitated under ether anesthesia, and CS was removed immediately. Thiobarbituric acid-reactive substances (TBARS) and nitric oxide (NO) levels as well as total superoxide dismutase (t-SOD) and xanthine oxidase (XO) enzyme activities in the CS were measured. Rats treated with ?-3 EFA had significantly lower values of TBARS ( Daha fazlası Daha az

Evaluation of lipid peroxidation, oxidant/antioxidant status, and serum nitric oxide levels in alopecia areata

Koca R. | Armutcu F. | Altinyazar H.C. | Gürel A.

Article | 2005 | Medical Science Monitor11 ( 6 ) , pp.253 - 259

Background: The pathophysiology of alopecia areata (AA) has not been clearly defined; however, it appears as a tissue-restricted autoimmune disease mediated by T lymphocytes. Immunohistochemical studies have shown peri- and infra-follicular inflammatory infiltrate which damages hair follicles. We analyzed the role of lipid peroxidation and oxidant-antioxidant enzymes in the pathogenesis of AA. Material/Methods: Twenty-four patients with AA and 20 age- and sex-matched healthy controls were enrolled in this study. We analyzed serum levels of malondialdehyde (MDA) and nitric oxide (NO) and the serum activities of superoxide dismutase ( . . .SOD) and xanthine oxidase (XO) in patients with AA and control subjects. Results: The levels of MDA and NO (nitrite/nitrate) and the activity of XO in serum of patients with AA (0.76±0.34 nmol/ml, 14.88±6.40 nmol/ml, and 0.34±0.10 U/ml, respectively) were significantly higher than those of controls (0.35±0.09 nmol/ml, 10.71±1.75 nmol/ml, 0.11±0.03 U/ml; Daha fazlası Daha az

Effects of taurine in glucose and taurine administration

Kaplan B. | Karabay G. | Zagyapan R.D. | Özer Ç. | Sayan H. | Duyar I.

Article | 2004 | Amino Acids27 ( 03.Apr ) , pp.327 - 333

Taurine has several biological processes such as hypoglycemic action, antioxidation, detoxification, etc. To assess the effect of taurine administration on the guinea pigs with hyperglycemia, blood glucose, C-peptide levels together with morphologic alterations in the pancreatic ultrastructure were investigated in terms of hypoglycemic action and malondialdehyde and total sulfhydryl group levels with regard to oxidation-antioxidation relation. Animals were divided into four groups of six. Glucose supplementation group was administrated a single dose of glucose (400 mg/kg, i.p.) injection. Glucose and taurine supplementation group wa . . .s administrated glucose treatment (a single dose, 400 mg/kg, i.p.) following taurine (a single dose, 200 mg/kg, i.p.). Taurine and glucose supplementation group was administered taurine treatment (a single dose, 200 mg/kg, i.p.) following glucose treatment (a single dose, 400 mg/kg, i.p.). Control animals received no treatment. Blood samples were collected at the end of the experiments for the determination of glucose, C-peptide (indicator of insulin secretion), lipid peroxidation (thiobarbituric acid reactive substances), and total sulfhydryl groups levels. Pancreatic tissue samples were then collected and processed for transmission electron microscopy. The findings showed that glucose supplementation following taurine administration significantly decreased blood glucose level by increasing C-peptide level and the pancreatic secretion stimulated morphologically and insignificantly changed thiobarbituric acid reactive substances and total sulfhydryl group levels. These observations suggest that taurine administration may be useful in hyperglycemia because of its hypoglycemic and protective effects. © Springer-Verlag 2004 Daha fazlası Daha az

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