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Koleksiyon [16]
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Lipid peroxidation and homocysteine levels in Behçet's disease

Mungan A.G. | Can M. | Açikgöz S. | Eştürk E. | Altinyazar C.

Article | 2006 | Clinical Chemistry and Laboratory Medicine44 ( 9 ) , pp.1115 - 1118

Background: The aim of this study was to investigate serum paraoxonase (PON1) activity in relation to homocysteine, malondialdehyde (MDA) and lipid parameters in active and inactive Behçet's disease (BD). Methods: A total of 46 consecutive BD patients and 25 healthy control subjects were included in the present study. Results: Serum PON1 activity in both active and inactive BD was significantly lower compared with healthy subjects (p

Role of caffeic acid phenethyl ester, an active component of propolis, against NAOH-induced esophageal burns in rats

Ocakci A. | Kanter M. | Cabuk M. | Buyukbas S.

Article | 2006 | International Journal of Pediatric Otorhinolaryngology70 ( 10 ) , pp.1731 - 1739

Objectives: This study was evaluated to investigate the efficacy of caffeic acid phenethyl ester (CAPE), which is a natural honeybee product exhibits a spectrum of biological activities including anti-microbial, anti-inflammatory, antioxidant and anti-tumoral actions, on the prevention of stricture development after esophageal caustic injuries in the rat. Methods: Thirty healthy male Wistar albino rats were utilized in this study. The rats were randomly allotted into one of three experimental groups: group A (sham) animals were uninjured. Caustic esophageal burn was created by applying 1 ml 37.5% NaOH to the distal esophagus. Group . . .B rats were injured but untreated. Group C rats were injured and received CAPE (10 µmol/kg/day i.p. for 28 days). Efficacy of the treatment was assessed by measuring the esophageal transit time, stenosis index, histopathologic damage score and biochemically by determining tissue hydroxyproline content, lipid peroxidation and antioxidant enzyme activities. Results: The esophageal transit time, the stenosis index, histopathologic damage score and the hydroxyproline level were significantly increased in the untreated group compared with the sham and CAPE-treated groups. Treatment with CAPE decreased tissue hydroxyproline levels, histological damage, and the stenosis index, but except the esophageal transit time. Caustic esophageal burn also increased the lipid peroxidation and decreased the antioxidant enzyme activities in the untreated group. CAPE treatments decreased the elevated lipid peroxidation and also increased the reduced antioxidant enzyme activities. In corrosive esophageal burn group with no treatment, the most consistent findings were degenerative changes and increased in submucosal collagen content, and the luminal narrowing. CAPE treatment protected esophagus. Nevertheless, there was the slight increase in submucosal collagen. Conclusions: It is concluded that CAPE has a preventive effect on the stricture development after esophageal caustic injuries in the rat. © 2006 Elsevier Ireland Ltd. All rights reserved Daha fazlası Daha az

Inhibitory effect of caffeic acid phenethyl ester on bleomycine-induced lung fibrosis in rats

Özyurt H. | Sögüt S. | Yildirim Z. | Kart L. | Iraz M. | Armutçu F. | Temel I.

Conference Object | 2004 | Clinica Chimica Acta339 ( 01.Feb ) , pp.65 - 75

Background: Pulmonary fibrosis (PF) induced by anticancerogenic bleomycin (BLM) is one of the more common side effects encountered during cancer treatment. It has been suggested in the last decades that the main responsible agent in PF is reactive oxygen species which were generated also in normal physiological conditions in the human body. In this experimental study, we investigated the preventive or attenuating effects of caffeic acid phenethyl ester (CAPE) that has been demonstrated to have anti-inflammatory, cytocytatic, anticancerogenic, antiprolipherative and antioxidant effects on BLM-induced PF. Methods: Thirty-six Sprague-D . . .awley rats were divided randomly into four groups as sham operation, BLM, BLM+vitamin E (vit E), and BLM+CAPE groups. BLM (7.5 mg/kg, single dose) was applied intratracheally, and CAPE and vit E intraperitoneally in the appropriate groups. At the end of the fibrosis processes, lung tissues were removed and the levels of tissues hydroxyproline (OH-proline), malondialdehyde (MDA) and NO as well as the activities of superoxide dismutase (SOD), catalase (CAT) and myeloperoxidase (MPO) were determined. Also, the weights of the rats were recorded at 7th and 14th days of the experiments. Results: BLM application to the rats resulted in a significant increase in the OH-proline level as compared to the controls. Administration of CAPE and vit E led to the remarkable reduction of total lung OH-proline levels compared to the rats treated with BLM alone ( Daha fazlası Daha az

Oxidant and antioxidant status of plasma and erythrocyte in the bleomycin-administered rats: The protective role of erdosteine and vitamin E

Armutçu F. | Sögüt S. | Gürel A. | Kart L. | Coşkun Ö.

Article | 2004 | THOD - Turk Hematoloji-Onkoloji Dergisi14 ( 4 ) , pp.205 - 213

Bleomycin (BLM) toxicity is associated with lipid peroxidation, which is an autocatalytic mechanism leading to oxidative destruction of cellular membranes, and their destruction can lead to the production of toxic, reactive metabolites and cell death. Therefore, we investigated reactive oxygen species production, antioxidant enzyme activities and protective effect of vitamin E and erdosteine in BLM-administrated rats. Thirty-five Sprague-Dawley rats were divided randomly into four groups as untreated control, BLM, BLM+erdosteine and BLM+vitamin E groups. At the end of the treatment, plasma and erythrocytes were obtained and the leve . . .ls of thiobarbituric acid reactive substances (TEARS) and nitric oxide (NO) as well as the activities of superoxide dismutase (SOD), catalase (CAT) and glutathion peroxidase (GSH-Px) were measured. Bleomycin administration resulted in the generation of reactive oxygen species in the blood of rats by decreasing the activities of enzymes SOD, CAT and GSH-Px, while increasing the levels of NO and TEARS, an indicative of lipid peroxidation. Erdosteine and vitamin E treatment prevented the increase in the lipid peroxidation. Erdosteine alone significantly prevented the decrease in SOD, CAT and GSH-Px activities in the erythrocyte and plasma. We suggest that erdosteine is more effective on the prevention of BLM-induced hematotoxicity via antioxidant and free radical scavenger properties than vitamin E at the doses used in the present study. However, further studies at different doses of erdosteine are needed to determine most appropriate doses and to clarify the issue Daha fazlası Daha az

Protection of endotoxin-induced oxidative renal tissue damage of rats by vitamin E or/and EGb 761 treatment

Coskun O. | Armutcu F. | Kanter M. | Kuzey G.M.

Article | 2005 | Journal of Applied Toxicology25 ( 1 ) , pp.8 - 12

The aim of the present study was to evaluate the possible protective effects of vitamin E and EGb 761 treatments, alone or in combination, against oxidative renal tissue damage in experimentally induced endotoxaemic rats. Fifty healthy male Wistar albino rats, weighing 150-250 g and averaging 12 weeks old, were allotted randomly into one of five experimental groups: A (untreated), B (endotoxaemic), C (endotoxaemic + vitamin E treated), D (endotoaxemic + EGb 761 treated) and E (endotoxaemic + vitamin E and EGb 761 treated), each containing ten animals. Group A received only an intraperitoneal (i.p.) injection of 2 ml of normal saline . . . solution and served as the control. Groups B, C, D and E were administrated a single i.p. injection of 0.5 ml of endotoxin solution. In addition, groups C, D and E received i.p. injections of 600 mg kg-1 body mt. of vitamin E and oral extract of 50 mg kg-1 body wt. of EGb 761, alone or in combination, immediately after the endotoxin injection. The experiment lasted for 24 h. At the end of the experiment blood and tissue samples were obtained for biochemical and histopathological investigation. Endotoxin injection produced renal damage, increased lipid peroxidation and decreased antioxidant enzyme activity. Vitamin E or/and EGb 761 treatment decreased lipid peroxidation, increased antioxidant enzyme activity and also prevented renal tissue damage in experimentally induced endotoxaemic rats. In conclusion, vitamin E and EGb 761 treatment, alone or in combination, appears to be beneficial in preventing endotoxin-induced oxidative renal tissue damage and therefore shows potential for clinical use. Copyright © 2005 John Wiley & Sons, Ltd Daha fazlası Daha az

Effects of in vivo antioxidant enzyme activities of myrtle oil in normoglycaemic and alloxan diabetic rabbits

Sepici-Dincel A. | Açikgöz S. | Çevik C. | Sengelen M. | Yeşilada E.

Article | 2007 | Journal of Ethnopharmacology110 ( 3 ) , pp.498 - 503

In this study we aimed to evaluate the in vivo effects of myrtle oil (myrtii oleum) on the antioxidant enzymes such as superoxide dismutase and catalase, the levels of malondialdehyde in liver tissues as an index of lipid peroxidation and nitrite-nitrate levels in normoglycaemic and alloxan-induced diabetic and MO-treated rabbits. In our previous study, we assumed that MO with a dose of 50 mg/kg, possesses a hypoglycemic activity and this activity was independent from the effects of insulin. Myrtle oil exerts its hypoglycemic activity by enhanced glycolysis, glycogenesis and decreased glycogenolysis. What is more glucose load data s . . .trongly suggest that MO treatment produces hypoglycemia mainly by reducing intestinal absorption of glucose, so MO could be an ?-glycosidase enzyme inhibitor which had a hypoglycaemic effect only on alloxan-induced diabetic rabbits on the fourth hour and on orally glucose loaded group. The major finding of this new study is that, MO may not offer any protection against oxidative stress during acute studies in normoglycemic and diabetic groups. Although the levels of superoxide dismutase and catalase enzyme activities did not change during acute studies in diabetes + MO group, there was a significant change at the end of 21 days. There is a very limited knowledge about MO and its effects on diabetes. Therefore, we tried to explain the mechanism that might underlie the protective effects of MO with this paper. © 2006 Elsevier Ireland Ltd. All rights reserved Daha fazlası Daha az

Evaluation of lipid peroxidation, oxidant/antioxidant status, and serum nitric oxide levels in alopecia areata

Koca R. | Armutcu F. | Altinyazar H.C. | Gürel A.

Article | 2005 | Medical Science Monitor11 ( 6 ) , pp.498 - 503

Background: The pathophysiology of alopecia areata (AA) has not been clearly defined; however, it appears as a tissue-restricted autoimmune disease mediated by T lymphocytes. Immunohistochemical studies have shown peri- and infra-follicular inflammatory infiltrate which damages hair follicles. We analyzed the role of lipid peroxidation and oxidant-antioxidant enzymes in the pathogenesis of AA. Material/Methods: Twenty-four patients with AA and 20 age- and sex-matched healthy controls were enrolled in this study. We analyzed serum levels of malondialdehyde (MDA) and nitric oxide (NO) and the serum activities of superoxide dismutase ( . . .SOD) and xanthine oxidase (XO) in patients with AA and control subjects. Results: The levels of MDA and NO (nitrite/nitrate) and the activity of XO in serum of patients with AA (0.76±0.34 nmol/ml, 14.88±6.40 nmol/ml, and 0.34±0.10 U/ml, respectively) were significantly higher than those of controls (0.35±0.09 nmol/ml, 10.71±1.75 nmol/ml, 0.11±0.03 U/ml; Daha fazlası Daha az

Potential role of dietary ?-3 essential fatty acids on some oxidant/antioxidant parameters in rats' corpus striatum

Sarsilmaz M. | Songur A. | Özyurt H. | Kuş I. | Özen O.A. | Özyurt B. | Sögüt S.

Conference Object | 2003 | Prostaglandins Leukotrienes and Essential Fatty Acids69 ( 4 ) , pp.253 - 259

Omega-3 (?-3) is an essential fatty acid (EFA) found in large amounts in fish oil. It contains eicosapentaenoic acid and docosahexaenoic acid (DHA). DHA is one of the building structures of membrane phospholipids of brain and necessary for continuity of neuronal functions. Evidences support the hypothesis that schizophrenia may be the result of increased reactive oxygen species mediated neuronal injury. Recent reports also suggest the protective effect of ?-3 EFA against neuropsychiatric disorders including schizophrenia. This study proposed to assess the changes in antioxidant enzyme and oxidant parameters in the corpus striatum (C . . .S) of rats fed with ?-3 EFA diet (0.4 g/kg/day) for 30 days. Eight control rats and nine rats fed with ?-3 were decapitated under ether anesthesia, and CS was removed immediately. Thiobarbituric acid-reactive substances (TBARS) and nitric oxide (NO) levels as well as total superoxide dismutase (t-SOD) and xanthine oxidase (XO) enzyme activities in the CS were measured. Rats treated with ?-3 EFA had significantly lower values of TBARS ( Daha fazlası Daha az

Antioxidant response at early stages and low grades of simple coal worker's pneumoconiosis diagnosed by high resolution computed tomography

Altin R. | Armutcu F. | Kart L. | Gurel A. | Savranlar A. | Özdemir H.

Article | 2004 | International Journal of Hygiene and Environmental Health207 ( 5 ) , pp.455 - 462

In miners exposed to coal dusts, coal worker's pneumoconiosis (CWP) can occur. The purpose of the present study is to better understand the relations between coal dust exposure and activities of blood plasma antioxidant enzymes, namely, Superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), and lipid peroxidation end product malondialdehyde (MDA) concentration in coal workers with early and low grade simple CWP diagnosed by high resolution computed tomography (HRCT). Forty-three coal workers who had profusions of 0/1-2/2 according to ILO 1980 chest X-ray (CXR) classification, 43 coal workers without CWP (control group 1) an . . .d 44 healthy subjects (control group 2) who were randomly selected from the population register or recruited from the hospital staff were enrolled. Coal workers were reevaluated by HRCT (Hosoda-Shida classification) due to its higher sensitivity than standard CXR. Then, blood plasma SOD and GSH-Px activities and MDA plasma levels were measured. CWP was found positive in 46 of 89 coal workers by HRCT evaluation. Profusion 0 (P0, CWP not present), profusion 1 (P1, early CWP) and profusion 2 (P2, low grade CWP) were found in 43, 23 and 19 of patients found to have CWP by HRCT, respectively. We had no worker with profusion 3 (P3). Complicated CWP was shown in four of 46 patients and thesecases were excluded as the study was restricted to early and low-grade pneumoconiosis. In respect to the plasma levels of MDA and plasma activities of SOD and GSH-Px, statistically significant differences were found between CWP cases and control groups ( Daha fazlası Daha az

Beneficial effects of Ebselen on corrosive esophageal burns of rats

Ocakci A. | Coskun O. | Tumkaya L. | Kanter M. | Gurel A. | Hosnuter M. | Uzun L.

Article | 2006 | International Journal of Pediatric Otorhinolaryngology70 ( 1 ) , pp.45 - 52

Aim: This study was evaluated to investigate the efficacy of Ebselen, which is an organoselenium compound and glutathione peroxidase mimic, on the prevention of stricture development after esophageal caustic injuries in the rat. Methods: Thirty healthy male Wistar albino rats were utilized in this study. The rats were randomly allotted into one of three experimental groups: group A (sham) animals were uninjured. Caustic esophageal burn was created by applying 1 ml of 37.5% NaOH to the distal esophagus. Group B rats were injured but untreated. Group C rats were injured and received Ebselen (10 mg/kg/day) via the oral route. Blood and . . . tissue samples for the biochemical and histopathological analysis were taken all rats at the end (28th day) of the experiment. Oxidative stress is believed to play a role in the pathogenesis of corrosive esophageal burns. To assess changes in the cellular antioxidant defense system, we measured the activities of antioxidant enzymes (such as glutathione peroxidase (GSHPx), superoxide dismutase (SOD), and catalase (CAT)) in esophagus homogenates. We also measured esophageal tissue malondialdehyde (MDA) levels, a marker of lipid peroxidation, to determine whether there is an imbalance between oxidant and antioxidant status. Efficacy of the treatment was assessed by measuring the stenosis index and histopathologic damage score and biochemically by determining tissue hydroxyproline content, lipid peroxidation and antioxidant enzyme levels. Results: The stenosis index in group B was significantly increased compared with group A and C (P < 0.05). The hydroxyproline level was significantly increased in group B compared with group A and C (P < 0.05). In group B, the histopathologic damage score was significantly higher than in group C (P < 0.05). Treatment with Ebselen decreased tissue hydroxyproline levels, histological damage, and the stenosis index. Caustic esophageal burn increased the lipid peroxidation and also decreased the antioxidant enzyme levels in group B. Ebselen treatments for 28 days decreased the elevated lipid peroxidation and also increased the reduced antioxidant enzyme levels. Live weights of the rats was significantly decreased in group B compared with group A and C (P < 0.05). Conclusion: It is concluded that Ebselen has a preventive effect in the development of fibrosis and decrease the lipid peroxidation, and increase the antioxidant defense system activity in an experimental model of corrosive esophagitis in rats. © 2005 Elsevier Ireland Ltd. All rights reserved Daha fazlası Daha az

The oxidative and morphological effects of high concentration chronic toluene exposure on rat sciatic nerves

Coskun O. | Oter S. | Korkmaz A. | Armutcu F. | Kanter M.

Article | 2005 | Neurochemical Research30 ( 1 ) , pp.33 - 38

This study was designed to investigate the effects of chronic toluene inhalation in high concentration on lipid peroxidation, antioxidant enzyme activities and ultrastructural changes in the sciatic nerves of rats. Male Wistar albino rats (150-250 g) were divided in two experimental groups: the control and the toluene treated group (n = 10 for each). Toluene treatment was performed by inhalation of 3000 ppm toluene, in a 8 h/day and 6 day/week order for 16 weeks. Blood and tissue samples were obtained for biochemical and histopathological investigation. The blood and sciatic nerves were assayed for toluene by gas chromatography. Tol . . .uene significantly increased blood and tissue malondialdehyde (MDA), and decreased tissue superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), but not tissue catalase (CAT) levels when compared with controls. Electron micrographs of sciatic nerve in the toluene group shows myelin destructions with onion-bulb and bubble form protrusion on the myelin sheath and axolemma border of myelinated axons. The area of injury on the myelin sheath were measured by Image-Pro Plus. Mean of the injury area were estimated 34% each myelin. These findings indicate that chronic toluene inhalation might be involved with free radical processes Daha fazlası Daha az

Neuroprotective effects of Ebselen on experimental spinal cord injury in rats

Kalayci M. | Coskun O. | Cagavi F. | Kanter M. | Armutcu F. | Gul S. | Acikgoz B.

Article | 2005 | Neurochemical Research30 ( 3 ) , pp.403 - 410

Spinal cord injury (SCI) results in rapid and significant oxidative stress. This study was aimed to investigate the possible beneficial effects of Ebselen in comparison with Methylprednisolone in experimental SCI. Thirty six Wistar albino rats (200-250 g) were divided in to six groups; A (control), B (only laminectomy), C (Trauma; laminectomy + spinal trauma), D (Placebo group; laminectomy + spinal trauma + serum physiologic), E (Methylprednisolone group; laminectomy + spinal trauma + Methylprednisolone treated), F (Ebselen group; laminectomy + spinal trauma + Ebselen treated), containing 6 rats each. Spinal cord injury (SCI) was pe . . .rformed by placement of an aneurysm clip, extradurally at the level of T11-12. After this application, group A, B and C were not treated with any drug. Group D received 1 ml serum physiologic. Group E received 30 mg/kg Methylprednisolone and, Group F received 10 mg/kg Ebselen intraperitoneally (i.p.). Rats were neurologically examined 24 h after trauma and spinal cord tissue samples had been harvested for both biochemical and histopathological evaluation. All rats were paraplegic after SCI except the ones in group A and B. Neurological scores were not different in traumatized rats than that of non-traumatized ones. SCI significantly increased spinal cord tissue malondialdehyde (MDA) and protein carbonyl (PC) levels and also decreased superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) enzyme activities compared to control. Methylprednisolone and Ebselen treatment decreased tissue MDA and PC levels and prevented inhibition of the enzymes SOD, GSH-Px and CAT in the tissues. However, the best results were obtained with Ebselen. In groups C and D, the neurons of the spinal cord tissue became extensively dark and degenerated with picnotic nuclei. The morphology of neurons in groups E and F were very well protected, but not as good as the control group. The number of neurons in the spinal cord tissues of the groups C and D were significantly less than the groups A, B, E and F. We concluded that the use of Ebselen treatment might have potential benefits in spinal cord tissue damage on clinical grounds. © 2005 Springer Science+Business Media, Inc Daha fazlası Daha az

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