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Erdosteine ameliorates neurological outcome and oxidative stress due to ischemia/reperfusion injury in rabbit spinal cord

Ege E. | Ilhan A. | Gurel A. | Akyol O. | Ozen S.

Article | 2004 | European Journal of Vascular and Endovascular Surgery28 ( 4 ) , pp.379 - 386

Objective. Oxygen-derived free radicals have been suggested as important in degeneration after spinal cord ischemia. The aim of this study was to investigate whether erdosteine has a protective effect against spinal cord ischemia during aortic cross clamping. Materials and methods. New Zealand White rabbits (n=21) were divided into three groups. In the ischemia/reperfusion group (I/R) (n=8), the infrarenal aorta of rabbits was cross clamped for 21 min and then reperfused. In erdosteine group, the administration of erdosteine solution (50 mg/kg) was started two days before aortic cross-clamping and rabbits (n=8) were subjected to isc . . .hemia and reperfusion. Animals in control group (n=5) underwent a surgical procedure similar to the other groups but the aorta was not clamped. The animals were sacrificed at 72 h and histopathological, and biochemical analyses were carried out on the lumbar spinal cords. Results. Erdosteine treatment was associated with improved neurological function in the postoperative period. Histopathological examination of spinal cord tissues in erdosteine group revealed changes consistent with mild ischemic injury, but rabbits in I/R group with paraplegia had total destruction of the motor neurons. Biochemical analyses of spinal cord tissues, in the I/R group, revealed a significant increase in the superoxide dismutase, xanthine oxidase, adenosine deaminase and myeloperoxidase activities, and a significant depletion in glutathione peroxidase activity when compared to that of control rabbits. Erdosteine treatment prevented the increase of all these enzymes except adenosine deaminase. Ischemia/reperfusion produced a significant increase in the tissue malondialdehyde levels. Ischemia/reperfusion-induced increments in malondialdehyde content of the spinal cord were significantly prevented by erdosteine treatment. Conclusions. The present study demonstrated that erdosteine treatment before aortic cross clamping ameliorates neurological outcome, neuronal injury and oxidative stress in the rabbit spinal cord. © 2004 Elsevier Ltd. All rights reserved Daha fazlası Daha az

Cardioprotective effect of Thymoquinone: A constituent of Nigella sativa L., against myocardial ischemia/reperfusion injury and ventricular arrhythmias in anaesthetized rats

Gonca E. | Kurt C.

Article | 2015 | Pakistan Journal of Pharmaceutical Sciences28 ( 4 ) , pp.1267 - 1273

Reperfusion of the ischemic myocardium causes the myocardial injury and life-threatening ventricular arrhythmias in human. This study aimed to investigate the effects of thymoquinone (TQ) on myocardial ischemia/reperfusion (I/R) injury and ischemia-and reperfusion-induced ventricular arrhythmias in anaesthetized rats. Adult male Wistar albino rats were divided into two groups, each containing a control and TQ-treated subgroups. In group I, the myocardial infarct size was determined by triphenyl tetrazolium chloride staining following 2-h reperfusion preceded by 30 min of ischemia. In group II, a 6-min myocardial ischemia was followe . . .d by a 10-min reperfusion. TQtreated subgroups were treated with TQ (10 mg/100µl/kg, i.p.) and the control subgroups were treated with the vehicle (100 µl/kg, i.p.) 20 min prior to the ischemic period. Ischemia was induced by ligating the left main coronary artery, followed by reperfusion. TQ treatment reduced the infarct size (15±4% versus 69±6%, Daha fazlası Daha az

Effect of dexmedetomidine on testicular torsion/detorsion damage in rats

Hanci V. | Erol B. | Bektaş S. | Mungan G. | Yurtlu S. | Tokgöz H. | Can M.

Article | 2010 | Urologia Internationalis84 ( 1 ) , pp.105 - 111

Background and Objective: We assessed the antioxidant activity of dexmedetomidine (DEX) during an ischemic period in a rat model of testicular torsion/detorsion (T/DT) by using biochemical and histopathological methods. Methods: Wistar Albino male rats weighing 250-300 g were divided into three groups: sham (group S, n = 7); torsion/detorsion (group T/DT, n = 7), and DEX treatment (group DEX, n = 7). In the T/DT group, right testes were rotated 720° for 1 h. Group S served for normal basal values. Rats in group T/DT were operated to make T/DT, this group served as a control group. Group DEX received intraperitoneal DEX 10 µg · kg-1 . . .after the 30-min torsion period. For measurement of total antioxidant enzyme activities and malondialdehyde (MDA) levels, testes of 7 animals in each group were excised after 4 h of reperfusion. Germ cell apoptosis was evaluated using the apoptosis protease-activating factor 1 (APAF-1) antibody in all groups and also on the expressions of endothelial nitric oxide synthase (eNOS) and inducible nitric oxide synthase (iNOS) were assessed within the bilateral testes. Results: Mean MDA levels in group T/DT were significantly higher than in groups S and DEX (p < 0.05). There were also significant decreases in mean total antioxidant activities in group T/DT when compared to groups S and DEX (p < 0.05). These values were significantly higher in group DEX than group T/DT. Germ cell apoptosis, eNOS and iNOS levels were significantly higher in group T/DT when compared to groups S and DEX (p < 0.05). Conclusions: DEX treatment has potential biochemical and histopathological benefits by preventing ischemia/reperfusion-related cellular damage in an experimental testicular torsion model. Preference of DEX for anesthesia during the detorsion procedure may attenuate ischemia-reperfusion injury. © 2010 S. Karger AG, Basel Daha fazlası Daha az

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