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Eicosanoids mediate cellular immune response and phenoloxidase reaction to viral infection in adult Pimpla turionellae

Büyükgüzel, Ender

Article | 2012 | Archives of Insect Biochemistry and Physiology81 ( 1 ) , pp.20 - 33

Nodulation is the predominant insect cellular immune response to microbial infections. We posed the hypothesis that parasitoid insects in their adulthood express melanotic nodulation reactions to viral challenge and that eicosanoids mediate nodulation reactions and phenoloxidase (PO) activation in response to viral challenge. To test this idea, we injected Pimpla turionellae adults with indomethacin, a nonsteroidal anti-inflammatory drug, immediately prior to intrahemocoelic injection of Bovine herpes simplex virus-1 (BHSV-1). Treating newly emerged adults of P. turionellae with BHSV-1 induced nodulation reactions, and decreased PO . . .activity at high viral doses. Relative to vehicle-treated controls, indomethacin-treated adults produced significantly reduced numbers of nodules following viral infection (down from approximately 21 nodules per adult to less than six nodules per adult). In addition to injection treatments, increasing dietary indomethacin dosages (from 0.01% to 0.1%) were associated with decreasing nodulation (by six-fold) and PO (by about three-fold) reactions to BHSV-1 injection. Wasp adults orally fed with the lowest dietary indomethacin concentration (0.001%) expressed significantly increased PO activity (1.45 unit/min/mg protein) while nodulation reaction was not affected in response to viral challenge compared to control adults. We infer from these findings that cyclooxygenase (COX) products, at least prostaglandins, mediate nodulation response and PO action to viral infection in adults of these highly specialized insects. © 2012 Wiley Periodicals, Inc Daha fazlası Daha az

Gastroprotective and antioxidant effects of montelukast on indomethacin-induced gastric ulcer in rats

Dengiz G.O. | Odabasoglu F. | Halici Z. | Cadirci E. | Suleyman H.

Article | 2007 | Journal of Pharmacological Sciences105 ( 1 ) , pp.94 - 102

Montelukast, a selective reversible cysteinyl leukotriene D4-receptor (LTD4 receptor) antagonist, is used in the treatment of asthma. We have investigated alterations in the glutathione (GSH) and activity levels of antioxidative enzymes [superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), and glutathione reductase (GR)] and myeloperoxidase (MPO), as markers of the ulceration process following oral administration of montelukast, lansoprazole, famotidine, and ranitidine, respectively, in rats with indomethacin-induced ulcers. In the present study, we found that 1) montelukast, lansoprazole, famotidine, and ran . . .itidine all reduced the development of indomethacin-induced gastric damage, with this reduction occurring at a greater magnitude for montelukast, famotidine, and lansoprazole than for ranitidine; 2) montelukast and ranitidine both alleviated increases in the activity levels of CAT and GST enzymes resulting from gastric injury; 3) montelukast and ranitidine both ameliorated depressions in the GSH and activity levels of SOD and GR enzymes caused by indomethacin administration; and 4) all doses of montelukast, lansoprazole, and ranitidine decreased amplification of MPO activity resulting from induced gastric injuries. These results suggest that the gastroprotective effects of montelukast on indomethacin-induced ulcerations can be attributed to its ameliorating effect on oxidative damage and MPO activity. ©2007 The Japanese Pharmacological Society Daha fazlası Daha az

Mitigation of indomethacin-induced gastric mucosal lesions by a potent specific type V phosphodiesterase inhibitor

Karakaya K. | Hanci V. | Bektas S. | Can M. | Ucan H.B. | Emre A.U. | Tascilar O.

Article | 2009 | World Journal of Gastroenterology15 ( 40 ) , pp.5091 - 5096

AIM: To investigate the gastroprotective effect of vardenafil against indomethacin-induced gastric damage. METHODS: Forty-eight female Wistar albino rats were randomly divided into 6 groups. Group 1 received saline only. Group 2 (indomethacin) received indomethacin. Rats in group 3 and 4 were pretreated with different doses of famotidine. Group 5 and 6 were pretreated with different doses of vardenafil. Rats in groups 3 to 6 received 25 mg/kg indomethacin 30 min after pretreatment. The animals were sacrificed 6 h later and their stomachs were opened. Gastric lesions were counted and measured. The stomach of each animal was divided i . . .n two parts for histopathological examinations and nitric oxide (NO) and malondialdehyde (MDA) assays, respectively. RESULTS: There were no gastric mucosal lesion in the saline group but all rats in the indomethacin group had gastric mucosal ulcerations (ulcer count; 6.25 ± 3.49, and mean ulcer area; 21.00 ± 12.35). Ulcer counts were diminished with famotidine 5 mg/kg (4.12 ± 2.47, P > 0.05), 20 mg/kg (2.37 ± 4.43, P < 0.05), vardenafil 2 mg/kg (4.37 ± 3.06), and vardenafil 10 mg/kg (1.25 ± 1.38, P < 0.05) compared to the indomethacin group. Gastric mucosal lesion areas were diminished with famotidine 5 mg/kg (8.62 ± 2.97, P < 0.001), famotidine 20 mg/kg (0.94 ± 2.06, P < 0.001), vardenafil 2 mg/kg (6.62 ± 5.87, P < 0.001), and vardenafil 10 mg/kg (0.75 ± 0.88, P < 0.001) compared to the indomethacin group. MDA levels were significantly higher in indomethacin group (28.48 ± 14.51), compared to the famotidine 5 mg/kg (6,21 ± 1.88, P < 0.05), famotidine 20 mg/kg (5.88 ± 1.60. P < 0.05), vardenafil 2 mg/kg (15.87 ± 3.93, P < 0.05), and vardenafil 10 mg/kg (10.97 ± 4.50, P < 0.05). NO concentration in gastric tissues of the famotidine groups were significantly increased (P < 0.05), but the NO increases in the vardenafil groups were not statistically significant. Histopathology revealed diminished gastric damage for pretreatment groups compared to the indomethacin group (P < 0.05). CONCLUSION: Vardenafil affords a significant dose-dependent protection against indomethacin induced gastric mucosal lesions in rats. © 2009 The WJG Press and Baishideng. All rights reserved Daha fazlası Daha az

Eicosanoids mediate Galleria mellonella cellular immune response to viral infection

Büyükgüzel, Ender | Tunaz , Hasan | Stanley, David | Büyükgüzel, Kemal

Article | 2007 | Journal of Insect Physiology53 ( 1 ) , pp.99 - 105

Nodulation is the predominant insect cellular immune response to bacterial and fungal infections and it can also be induced by some viral infections. Treating seventh instar larvae of greater wax moth Galleria mellonella with Bovine herpes simplex virus-1 (BHSV-1) induced nodulation reactions in a dose-dependent manner. Because eicosanoids mediate nodulation reactions to bacterial and fungal infection, we hypothesized that eicosanoids also mediate nodulation reactions to viral challenge. To test this idea, we injected G. mellonella larvae with indomethacin, a nonsteroidal anti-inflammatory drug immediately prior to intrahemocoelic i . . .njection of BHSV-1. Relative to vehicle-treated controls, indomethacin-treated larvae produced significantly reduced numbers of nodules following viral infection (down from approximately 190 nodules/larva to Daha fazlası Daha az

Hyperkalemia occurring in a patient with psoriatic arthritis following indomethacin use

Sayarlioglu H. | Atmaca H. | Unalacak M. | Dogan E. | Erkoc R.

Article | 2005 | Journal of Applied Research5 ( 2 ) , pp.295 - 298

Objective: To report a case of hyperkalemia possibly due to indomethacin use. Case Summary: A 52-year-old white woman with psoriatic arthritis for 16 years and diabetes mellitus for 3 years was admitted to the university hospital due to swelling and pain of wrists, elbows, knees and ankles for the last one month. The patient had been receiving methotrexate irregularly, but discontinued it 3 months ago. Physical examination and laboratory evaluations were compatible with diagnosis of exacerbation of psoriatic arthritis and type 2 diabetes mellitus. Two days after initiation of indomethacin and methotrexate, hyperkalemia developed wit . . .h increase of blood urea nitrogen and decrease of creatinine clearance. Indomethacin was discontinued, and this resulted in normalization of laboratory findings between day 5 and 10 after discontinuation. Conclusion: The development of hyperkalemia caused by indomethacin is probably unusual, but it is important because indomethacin is a commonly used medication. This potentially serious complication can be prevented by careful attention to renal function and potassium balance in patients receiving indomethacin and other nonsteroidal anti-inflammatory drugs (NSAIDs), especially in patients with type 2 diabetes mellitus or preexisting renal disease Daha fazlası Daha az

The effect of indomethacin on hyperoxaluria-induced renal tubular epithelial injury [Hiperoksalürinin neden oldugu renal tübüler epiteliyal hasar üzerine indometazinin etkisi]

Yencilek F. | Erturhan S. | Cangüven Ö. | Erol B. | Koyuncu H. | Göktaş C. | Sarica K.

Article | 2009 | Turk Uroloji Dergisi35 ( 4 ) , pp.298 - 303

Objective: The aim of this study was to determine the effect of indomethacin, an anti-inflammatory agent, on apoptosis and crystal deposition developing as a consequence of tubular cell injury induced by hyperoxaluria in an animal model. Materials and methods: Fifty New Zealand rabbits were divided into 3 groups. The first 2 groups were fed with hyperoxaluric diet and Group 3 was the control group with no supplementary procedure or treatment. While the animals in Group 1 were given only hyperoxaluric diet, Group 2 animals was applied indomethacin in addition to the hyperoxaluric diet. Animals were sacrificed at the early (7th day) a . . .nd late (28th day) periods and renal tis-sue specimens were sent for the pathological analysis of crystal deposition and apoptosis. Results: The presence and degree of crystal deposition were significantly less in the specimens obtained from indomethacin-treated group during both the early and late periods ( Daha fazlası Daha az

Gastroprotective and antioxidant effects of amiodarone on indomethacin-induced gastric ulcers in rats

Ozbakis Dengiz G. | Odabasoglu F. | Halici Z. | Suleyman H. | Cadirci E. | Bayir Y.

Article | 2007 | Archives of Pharmacal Research30 ( 11 ) , pp.1426 - 1434

Reactive oxygen species (ROS) have been implicated in the etiology of indomethacin-induced gastric mucosal damage. This study investigated amiodarone's protective effects against oxidative gastric mucosal damage induced by indomethacin. Amiodarone is a widely used antiarrhythmic agent. We have investigated alterations in the glutathione level, and the activities of antioxidative enzymes [superoxide dismutase, catalase, glutathione s-transferase glutathione reductase and myeloperoxidase], as markers for ulceration process following oral administration of amiodarone and ranitidine in rats with indomethacin-induced ulcers. In the prese . . .nt study we found that 1) amiodarone, lansoprazole and ranitidine reduced the development of indomethacin-induced gastric damages, at a greater magnitude for amiodarone and lansoprazole than for ranitidine; 2) amiodarone and ranitidine alleviated increases in the activities of catalase and glutathione s-transferase enzymes resulting from ulcers; 3) amiodarone and ranitidine ameliorated depressions in the glutathione level and the activities of superoxide dismutase and glutathione reductase enzymes caused by indomethacin administration; and 4) all doses of amiodarone amplified the myeloperoxidase activity resulting from indomethacin-induced gastric ulcers. The results indicate that the gastroprotective activity of amiodarone, which may be linked to its intrinsic antioxidant properties, cannot be attributed to its effect on myeloperoxidase activity Daha fazlası Daha az

Histopathologic evaluation of anti-ulcerogenic effect of montelukast in indomethacin-induced experimental ulcer model

Özbakiş-Dengiz G. | Çadirci E. | Yurdakan G.

Article | 2013 | Turkish Journal of Gastroenterology24 ( 2 ) , pp.88 - 92

Background/aims: The effects of anti-ulcerogenic drugs are dependent on the increase in prostaglandin production and reduction in leukotriene production in the gastric mucosa. Montelukast is an anti-asthmatic drug, a selective reversible cysteinyl leukotriene D4 receptor antagonist. In this study, we aimed to evaluate the anti-ulcerogenic effect of montelukast and to investigate the relationship between its anti-ulcerogenic effect and polymorphonuclear leukocyte infiltration in the gastric tissues. Materials and Methods: Male Sprague-Dawley rats were separated into five groups. Distilled water (control group), famotidine (40 mg/kg), . . . and montelukast (5, 10 and 20 mg/kg) were given orally (gavage). Thirty minutes later, indomethacin (25 mg/kg) was administered to all the groups. Six hours later, the animals were sacrificed by decapitation. The ulcer indexes for each stomach and the ulcer inhibition rates for each group were calculated, and the stomachs were later evaluated histopathologically (polymorphonuclear leukocyte infiltration). Results: Ulcer inhibition rates were as follows: famotidine 96.14% and montelukast 59.96%, 72.65% and 76.97% (5, 10 and 20 mg/kg, respectively). Montelukast (10 and 20 mg/kg) showed effects similar to those of famotidine histopathologically. Conclusions: In this study, it was observed that there was a relationship between the anti-ulcerogenic effect of montelukast and polymorphonuclear leukocyte infiltration in the gastric mucosa, and montelukast behaved as an anti-ulcerogenic drug both macroscopically and microscopically Daha fazlası Daha az

Effects of statins in an indomethacin-induced gastric injury model in rats

Özbakiş-Dengiz G. | Hekimoglu A. | Kandemir N. | Kurcer Z.

Article | 2012 | Turkish Journal of Gastroenterology23 ( 5 ) , pp.456 - 462

Background/aims: Statins have additional pleiotropic effects beyond their lipid-lowering effects. In this study, the effects of statins were evaluated in an indomethacin-induced gastric injury model in rats. Materials and Methods: Animals were divided into eight groups. Distilled water (control group), omeprazole (30 mg/kg), atorvastatin (20 and 40 mg/kg), simvastatin (20 and 40 mg/kg), and rosuvastatin (20 and 40 mg/kg) were given orally (gavage). Thirty minutes later, indomethacin (25 mg/kg) was administered orally to all groups. Six hours later, the animals were sacrificed by decapitation. The mean ulcer indexes for each group we . . .re calculated, and the stomachs were evaluated histopathologically. Results: The ulcer indexes were as follows: control 1.72±0.16, omeprazole 0±0.00, and atorvastatin, simvastatin and rosuvastatin (at 20 and 40 mg/kg doses, respectively) 4.28±0.39, 4.99±0.96, 1.72±0.73, 1.90±0.48, 1.85±0.26, and 1.67±0.18. Atorvastatin significantly increased the indomethacin-induced ulcer index at both doses and the erosion score at 40 mg/kg dose. Although the 20 mg/kg dose of simvastatin inhibited mononuclear leukocyte infiltration, the 40 mg/kg dose induced hyperemia. Rosuvastatin did not decrease mononuclear leukocyte or neutrophil infiltrations at 20 mg/kg dose, and only neutrophil infiltration at the 40 mg/kg dose. Conclusions: In patients with gastric discomfort, statins must be used carefully. If statin therapy is needed, we recommend to avoid using atorvastatin and to use the other statins only in the minimum effective dose Daha fazlası Daha az

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