Hekimoglu A. | Kurcer Z. | Aral F. | Baba F. | Sahna E. | Atessahin A.

Article | 2009 | Tohoku Journal of Experimental Medicine218 ( 2 ) , pp.141 - 147

Testicular torsion is a common syndrome that could lead to infertility. We investigated the therapeutic effects of lycopene, an antioxidant caretenoid, on testicular ischemia/reperfusion (IR) injury that resembles testicular torsion. Male Wistar albino rats were divided into three groups: sham (n = 6), IR (n = 18), and ischemia/reperfusion with lycopene (IRL, n = 18). Left testicular artery and vein was occluded for 1 h, followed by reperfusion of 3 h, 24 h or 30 days in IR and IRL animals. Either corn oil (vehicle) or lycopene (4 mg/kg) was administrated once daily by gavage to IR or IRL animals, respectively, 5 min after ischemia. . . . Sham-operated animals were treated with vehicle by gavage 5 min after the operation. IR decreased sperm motility and concentration in both ipsilateral and contralateral testes and increased abnormal sperm rate in ipsilateral testis after 30 days of reperfusion. Treatment with lycopene increased the motility in bilateral testes and decreased the rate of abnormal sperm in ipsilateral testis to the sham level, but did not increase sperm concentration in bilateral testes. IR increased the activities of catalase and glutathione peroxidase and the level of reduced glutathione by 24 h of reperfusion, but malondialdehyde remained unchanged. Lycopene treatment restored the enzyme activities but not the reduced glutathione level. Lycopene treatment also ameliorated the IR-induced tissue damage in bilateral testes. In conclusion, the therapeutic antioxidant effect of lycopene on germ cells could serve as a promising intervention to oxidative stress-associated infertility problems, such as testicular torsion. © 2009 Tohoku University Medical Press Daha fazlası Daha az

Ermis B. | Ors R. | Yildirim A. | Tastekin A. | Kardas F. | Akcay F.

Article | 2004 | Annals of Clinical and Laboratory Science34 ( 4 ) , pp.405 - 409

This cohort study investigated postnatal serum malondialdehyde (MDA), Superoxide dismutase (SOD), and glutathione peroxidase (GPx) levels in 14 active-smoking, 14 passive-smoking, and 15 non-smoking mothers and their newborns on day 7 post-partum. No significant differences were noted among the study groups with respect to MDA (p = 0.63) or SOD levels (p = 0.98) in either the mothers or their infants. However, there were significant differences among the study groups with respect to serum GPx activities in both the mothers (p = 0.028) and the infants (p = 0.039). When GPx activities were analyzed separately in both mothers and infan . . .ts, a significant difference was noted only between the infants of smoking mothers and the infants of non-smoking mothers (p = 0.015). In conclusion, there was a significant increase in GPx activities of smoking mothers and their infants, suggesting that they may have been exposed to more oxidant stress Daha fazlası Daha az

Ermis B. | Yildirim A. | Örs R. | Tastekin A. | Ozkan B. | Akcay F.

Article | 2005 | Biological Trace Element Research105 ( 01.Mar ) , pp.27 - 36

The aim of the study was to investigate simultaneously serum and milk malondialdehyde (MDA) levels, superoxide dismutase (SOD), glutathione peroxidase (GPx) activities, and antioxidant potential (AOP) in active-smoking, passive-smoking, and nonsmoking mothers and to search if there is any difference between serum and milk oxidant/ antioxidant status caused by smoking. According to their smoking status, 60 mothers (age range: 20-35 yr) were classified into one of three groups: the active-smoking mothers (n=15), the passive-smoking mothers (n=22), and the nonsmoking mothers (n=23). Serum and milk MDA, SOD, GPx, and AOP values were det . . .ermined in mothers on the postpartum seventh day by the spectrophotometric method. Serum Zn and Cu concentrations were determined by atomic absorption spectrophotometry (AAS). There was no significant difference in serum samples with respect to MDA (p=0.17), SOD (p=0.51), and AOP (p=0.36) levels, but there was a significant difference in serum GPx (p=0.002) levels among the study groups. The significant differences were also found in milk samples in terms of MDA (p=0.002) and SOD (p=0.011), but not in GPx (p=0.11) and AOP (p=0.29) levels among the study groups. No significant difference was seen in serum zinc concentration (p=0.49), but copper concentration differed significantly among the groups (p=0.005). These observations suggest that human milk is more vulnerable to oxidative stress and lipid peroxidation than serum samples in smoking mothers, even if they are passive smokers. © Copyright 2005 by Humana Press Inc Daha fazlası Daha az

Gul S. | Bahadir B. | Hanci V. | Acikgoz S. | Bektas S. | Ugurbas E. | Ankarali H.

Article | 2010 | Journal of Clinical Neuroscience17 ( 5 ) , pp.608 - 611

We investigated the effect of ebselen relative to nimodipine in an animal model of subarachnoid hemorrhage. Thirty Wistar albino rats were divided into 5 groups: G1, no intervention; G2, sham surgery without subarachnoid hemorrhage (SAH); G3, SAH only; G4, SAH plus nimodipine treatment; G5, SAH plus ebselen treatment. For G2 animals, physiological saline (0.9% NaCl) was injected into the cisterna magna. For G3, G4 and G5 animals, SAH was induced by injecting autologous non-heparinized blood into the cisterna magna. One hour after injection, G4 animals received nimodipine at 6-hour intervals and G5 animals received ebselen twice a da . . .y for 48 hours. After treatment, brain tissue and blood samples were taken for biochemical and histopathological examination. Mean malonyldialdehyde concentration was significantly higher in G3 than in G1 (p < 0.0001), G2 (p = 0.01), G4 (p = 0.002) and G5 (p = 0.014), and significantly higher in G5 than in G1 (p = 0.013). Mean superoxide dismutase activity was significantly lower in G4 than in both G1 (p = 0.025) and G2 (p = 0.02). Mean wall thickness was significantly greater in G3 than in G1 (p < 0.0001), G2 (p = 0.01), G4 (p < 0.0001) and G5 (p < 0.0001). Mean wall thickness was also significantly greater in both G1 and G2 than in G4 (p < 0.0014 and p < 0.0001) and G5 (p < 0.0001 and p < 0.0001). Mean luminal diameter of the basilar artery was significantly smaller in G3 than in G2 (p = 0.02), G4 (p < 0.018) and G5 (p < 0.001). Our results confirm that ebselen may have neuroprotective effects by acting to prevent vasospasm. © 2009 Elsevier Ltd. All rights reserved Daha fazlası Daha az