Alpay, Atilla | Bektas, Sibel | Alpay, Aysegul | Ugurbas, Silay Canturk | Evren, Cenk | Ugurbas, Suat Hayri

Article | 2012 | CUTANEOUS AND OCULAR TOXICOLOGY31 ( 2 ) , pp.128 - 131

Purpose: To investigate the histopathological changes due to administration of Ankaferd Blood Stopper (R) (ABS) into intraocular tissues by an anterior chamber and intravitreal injections. Methods: Twenty Wistar albino rats were divided into four equal groups. Group 1 was injected 0.01 mL ABS into anterior chamber. Group 2 was injected intravitreal 0.02 mL ABS. Groups 3 and 4, which were used as controls, were injected into the anterior chamber and intravitreal 0.01 mL and 0.02 mL balanced salt solution (BSS), respectively. At 2, 5, 10, 15 and 20 days after injection, the eyes were examined under an operating microscope and were sub . . .sequently enucleated for histopathological examination. Results: Ophthalmic examination of the rats prior to enucleation revealed ocular complications ranging from conjunctival hyperemia to corneal perforation in group 1 and increased conjunctival hyperemia and discharge in group 2. No physical and histopathological anomalies were detected in groups 3 and 4. All eyes in group 1 showed mixed type inflammatory cell reaction, foreign-body reaction, stromal congestion, disintegration of the collagen fibers and loss of the epithelium of the posterior wall in the iris and ciliary body were observed histopathologically. All eyes in group 2 showed disintegration and separation of the retina, brown pigment accumulation and mixed type inflammatory cell reaction. Conclusion: Our results indicate that the commercially available form of ABS solution exerts a toxic effect on intraocular tissues. We consider that the intraocular use of different concentrations, rather than multiple time point of ABS should be investigated Daha fazlası Daha az

Alpay, Atilla | Evren, Cenk | Bektas, Sibel | Ugurbas, Silay Canturk | Ugurbas, Suat Hayri | Cinar, Fikret

Article | 2011 | CUTANEOUS AND OCULAR TOXICOLOGY30 ( 4 ) , pp.280 - 285

Purpose: To investigate the effects of Ankaferd Blood Stopper (R) (ABS) on the ocular surface. Methods: Twenty adult male Wistar albino rats, weighing 390-530 g, were used in this prospective, controlled trial. One drop of ABS and one drop of balanced salt solution (BSS) were instilled into the lower conjunctival sac of the right and left eyes, respectively. After the rats were anesthetized, the ocular surface was evaluated based on the Draize criteria, and fluorescein tests were performed at 1, 2, 4, 18, 24, and 48 h. Subsequently, the rats were killed and all eyes were enucleated for histopathological examination. Results: The out . . .come of the Draize and fluorescein tests revealed that ABS caused more irritation of the ocular surface than BSS (P < 0.001). The highest mean ABS score was 4.9 for the Draize test and 0.4 for the fluorescein test, and ABS was considered to be a slight irritant. Histopathological examinations of the cornea and the conjunctiva revealed no significant difference between the eyes instilled with BSS and those instilled with ABS. Conclusions: ABS is a hemostatic drug that exerts a slight toxic effect on the ocular surface. Given its ease of use and antibacterial activity, as well as its efficiency in stopping bleeding, the use of ABS during ocular surgery should be further investigated in experimental and clinical studies Daha fazlası Daha az