Eicosanoids in insect immunity: Bacterial infection stimulates hemocytic phospholipase A(2) activity in tobacco hornworms
Intracellular phospholipase A(2) (PLA(2)) is responsible for releasing arachidonic acid from cellular phospholipids, and is thought to be the first step in eicosanoid biosynthesis. Intracellular PLA(2)s have been characterized in fat body and hemocytes from tobacco hornworms, Manduco sexta. here we show that bacterial challenge stimulated increased PLA(2) activity in isolated hemocyte preparations, relative to control hemocyte preparations that were challenged with water. The increased activity was detected as early as 15 s post-challenge and lasted for at least 1 h. The increased activity depended on a minimum bacterial challenge dose, and was inhibited in reactions conducted in the presence of oleyoxyethylphosphorylcholine, a site-specific PLA(2) inhibitor. In independent experiments with serum prepared from whole hemolymph, we found no PLA(2) activity was secreted into serum during the first 24 h following bacterial infection. We infer that a hemocytic intracellular PLA(2) activity is increased immediately an infection is detected. The significance of this enzyme lies in its role in launching the biosynthesis of eicosanoids, which mediate cellular immune reactions to bacterial infection.
| Yazar |
Tunaz, Hasan Park, Youngjin Büyükgüzel, Kemal Bedick, Job C. Aliza, A.R. Nor Stanley, David W. |
| Yayın Türü | Article |
| Tek Biçim Adres | https://hdl.handle.net/20.500.12628/2525 |
| Konu Başlıkları |
eicosonoids
insect immunity bacterial infection phospholiphose A(2) |
| Koleksiyonlar |
Fakülteler Fen Edebiyat Fakültesi Biyoloji Bölümü Makale Koleksiyonu (Biyoloji Bölümü) Araştırma Çıktıları | WoS | Scopus | TR-Dizin | PubMed | SOBİAD PubMed İndeksli Yayınlar Koleksiyonu Scopus İndeksli Yayınlar Koleksiyonu WoS İndeksli Yayınlar Koleksiyonu |
| Dergi Adı | ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY |
| Dergi Cilt Bilgisi | 52 |
| Dergi Sayısı | 1 |
| Sayfalar | 1 - 6 |
| Yayın Yılı | 2003 |
| Eser Adı [dc.title] | Eicosanoids in insect immunity: Bacterial infection stimulates hemocytic phospholipase A(2) activity in tobacco hornworms |
| Yazar [dc.contributor.author] | Tunaz, Hasan |
| Yazar [dc.contributor.author] | Park, Youngjin |
| Yazar [dc.contributor.author] | Büyükgüzel, Kemal |
| Yazar [dc.contributor.author] | Bedick, Job C. |
| Yazar [dc.contributor.author] | Aliza, A.R. Nor |
| Yazar [dc.contributor.author] | Stanley, David W. |
| Yayın Yılı [dc.date.issued] | 2003 |
| Yayıncı [dc.publisher] | WILEY-LISS |
| Yayın Türü [dc.type] | article |
| Özet [dc.description.abstract] | Intracellular phospholipase A(2) (PLA(2)) is responsible for releasing arachidonic acid from cellular phospholipids, and is thought to be the first step in eicosanoid biosynthesis. Intracellular PLA(2)s have been characterized in fat body and hemocytes from tobacco hornworms, Manduco sexta. here we show that bacterial challenge stimulated increased PLA(2) activity in isolated hemocyte preparations, relative to control hemocyte preparations that were challenged with water. The increased activity was detected as early as 15 s post-challenge and lasted for at least 1 h. The increased activity depended on a minimum bacterial challenge dose, and was inhibited in reactions conducted in the presence of oleyoxyethylphosphorylcholine, a site-specific PLA(2) inhibitor. In independent experiments with serum prepared from whole hemolymph, we found no PLA(2) activity was secreted into serum during the first 24 h following bacterial infection. We infer that a hemocytic intracellular PLA(2) activity is increased immediately an infection is detected. The significance of this enzyme lies in its role in launching the biosynthesis of eicosanoids, which mediate cellular immune reactions to bacterial infection. |
| Açıklama [dc.description] | WOS: 000180202600001 |
| Açıklama [dc.description] | PubMed: 12489129 |
| Kayıt Giriş Tarihi [dc.date.accessioned] | 2019-12-23 |
| Açık Erişim Tarihi [dc.date.available] | 2019-12-23 |
| Yayın Dili [dc.language.iso] | eng |
| Konu Başlıkları [dc.subject] | eicosonoids |
| Konu Başlıkları [dc.subject] | insect immunity |
| Konu Başlıkları [dc.subject] | bacterial infection |
| Konu Başlıkları [dc.subject] | phospholiphose A(2) |
| Künye [dc.identifier.citation] | unaz, H., Park, Y., Büyükgüzel, K., Bedick, J. C., Aliza, A. R. N. ve Stanley, D. W. (2003). Eicosanoids in insect immunity: Bacterial infection stimulates hemocytic phospholipase A2 activity in tobacco hornworms. Archives of Insect Biochemistry and Physiology, 52(1), 1-6. doi:10.1002/arch.10056 |
| Haklar [dc.rights] | info:eu-repo/semantics/closedAccess |
| ISSN [dc.identifier.issn] | 0739-4462 |
| İlk Sayfa Sayısı [dc.identifier.startpage] | 1 |
| Son Sayfa Sayısı [dc.identifier.endpage] | 6 |
| Dergi Adı [dc.relation.journal] | ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY |
| Dergi Sayısı [dc.identifier.issue] | 1 |
| Dergi Cilt Bilgisi [dc.identifier.volume] | 52 |
| Tek Biçim Adres [dc.identifier.uri] | https://dx.doi.org/10.1002/arch.10056 |
| Tek Biçim Adres [dc.identifier.uri] | https://hdl.handle.net/20.500.12628/2525 |
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