N-acetylcycsteine attenuates the deleterious effects of radiation therapy on incisional wound healing in rats

Background: During preoperative radiotherapy, effective doses of ionizing radiation occasionally cause wound complications after subsequent surgery. This study was designed to determine the effects of intraperitoneally or orally administered N-acetylcysteine (NAC) on anastomotic healing of irradiated rats. Material & Methods: Forty Wistar albino rats were randomized into four groups containing 10 rats each. A 3 cm long surgical full-thickness midline laparotomy was performed to all groups (Groups 1-4). Group 1 was designed as a control group without radiation therapy and NAC treatment. Groups 2, 3 and 4 received a single abdominal dose of 10 Gy irradiation before laparotomy and groups 3 and 4 received oral and intraperitoneal NAC, respectively. Results: Group comparisons demonstrated that breaking strength was significantly higher in NAC treated rats. A statistically significant difference was determined in terms of superoxide dismutase (SOD), malondealdehyde (MDA) and glutation (GSH) values between groups (p<0.001). Nevertheless, advanced oxidation protein products (AOPP) levels were found to be similar between groups (p=0.163). Serum GSH and SOD levels were significantly higher in groups 3 and 4 when compared to group 2 (p < 0.05). Similarly, there was a significant increase in serum MDA concentration, predicting lipid peroxidation, in group 2 when compared to groups 1, 3 and 4 (p < 0.05). There was not a significant difference between Groups 3 and 4 regarding GSH, MDA, SOD, and AOPP levels. Histopathological analysis revealed that NAC administration, either orally or intraperitoneally, leads to a better incisional healing in terms of inflammation, granulation, collagen deposition, reepithelization and neovascularization. Conclusion: The present study supports the hypothesis that NAC administration alleviates the negative effects of radiotherapy on incisional wound healing by means of reducing oxidative stress markers and improving histologic parameters independent of the route of administration.

Dergi Adı Hippokratia
Dergi Cilt Bilgisi 18
Dergi Sayısı 1
Sayfalar 17 - 23
Yayın Yılı 2014
Eser Adı
[dc.title]
N-acetylcycsteine attenuates the deleterious effects of radiation therapy on incisional wound healing in rats
Yazar
[dc.contributor.author]
Tascilar O.
Yazar
[dc.contributor.author]
Çakmak G.K.
Yazar
[dc.contributor.author]
Emre A.U.
Yazar
[dc.contributor.author]
Bakkal, Bekir Hakan
Yazar
[dc.contributor.author]
Kandemir N.
Yazar
[dc.contributor.author]
Turkcu U.O.
Yazar
[dc.contributor.author]
Demir E.O.
Yayın Yılı
[dc.date.issued]
2014
Yayıncı
[dc.publisher]
Hippokratia General Hospital of Thessaloniki
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
Background: During preoperative radiotherapy, effective doses of ionizing radiation occasionally cause wound complications after subsequent surgery. This study was designed to determine the effects of intraperitoneally or orally administered N-acetylcysteine (NAC) on anastomotic healing of irradiated rats. Material & Methods: Forty Wistar albino rats were randomized into four groups containing 10 rats each. A 3 cm long surgical full-thickness midline laparotomy was performed to all groups (Groups 1-4). Group 1 was designed as a control group without radiation therapy and NAC treatment. Groups 2, 3 and 4 received a single abdominal dose of 10 Gy irradiation before laparotomy and groups 3 and 4 received oral and intraperitoneal NAC, respectively. Results: Group comparisons demonstrated that breaking strength was significantly higher in NAC treated rats. A statistically significant difference was determined in terms of superoxide dismutase (SOD), malondealdehyde (MDA) and glutation (GSH) values between groups (p<0.001). Nevertheless, advanced oxidation protein products (AOPP) levels were found to be similar between groups (p=0.163). Serum GSH and SOD levels were significantly higher in groups 3 and 4 when compared to group 2 (p < 0.05). Similarly, there was a significant increase in serum MDA concentration, predicting lipid peroxidation, in group 2 when compared to groups 1, 3 and 4 (p < 0.05). There was not a significant difference between Groups 3 and 4 regarding GSH, MDA, SOD, and AOPP levels. Histopathological analysis revealed that NAC administration, either orally or intraperitoneally, leads to a better incisional healing in terms of inflammation, granulation, collagen deposition, reepithelization and neovascularization. Conclusion: The present study supports the hypothesis that NAC administration alleviates the negative effects of radiotherapy on incisional wound healing by means of reducing oxidative stress markers and improving histologic parameters independent of the route of administration.
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Konu Başlıkları
[dc.subject]
Incisional healing
Konu Başlıkları
[dc.subject]
Ionizing irradiation
Konu Başlıkları
[dc.subject]
Lipid peroxidation
Konu Başlıkları
[dc.subject]
N-acetylcysteine
Konu Başlıkları
[dc.subject]
Radiotherapy
Haklar
[dc.rights]
info:eu-repo/semantics/openAccess
ISSN
[dc.identifier.issn]
1108-4189
İlk Sayfa Sayısı
[dc.identifier.startpage]
17
Son Sayfa Sayısı
[dc.identifier.endpage]
23
Dergi Adı
[dc.relation.journal]
Hippokratia
Dergi Sayısı
[dc.identifier.issue]
1
Dergi Cilt Bilgisi
[dc.identifier.volume]
18
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/6710
Tek Biçim Adres
[dc.identifier.uri]
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103035/
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groups healing administration levels between significant incisional Groups compared received laparotomy significantly higher orally intraperitoneally effects radiation designed radiotherapy difference oxidation Nevertheless advanced increase protein products (AOPP) Similarly similar Histopathological reducing present supports hypothesis alleviates
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