Apoptosis and necrosis in the circumventricular organs after experimental subarachnoid hemorrhage as detected with annexin V and caspase 3 immunostaining

Objectives: The circumventricular organs (CVOs) are essential for most autonomic and endocrine functions. Trauma and bleeding can affect their function. The aim of this study was to investigate apoptosis and necrosis in CVOs in the early period after experimental subarachnoid hemorrhage (SAH) in rats, using annexin V affinity and caspase 3 immunostaining.Methods: Three experimental groups were used: Days 1 and 2 after SAH, and a control group, seven Wistar albino rats each. Subarachnoid hemorrhage was accomplished by transclival basilar artery puncture. Rats were perfused with 0.9%NaCl and 0.1M phosphate buffer pH 7.4 until heart stoppage. Apoptosis and necrosis in CVOs were measured by flow cytometry with annexin V staining, and by caspase 3 immunostaining.Results: Apoptosis in the organum vasculosum lamina terminalis (OVLT), median eminence (ME), and area postrema (AP) was significantly higher in the Day 1 group than in the control group. Apoptosis in the subfornicial organ (SFO), OVLT, ME, and AP was significantly higher in the Day 2 group than in the control group. There were significant differences between the Day 1 and Day 2 groups, except for AP. Necrosis in SFO and OVLT was significantly higher in the Day 2 group than in the Day 1 or control groups, whereas necrosis in the ME and AP did not differ between the three groups. Caspase 3-positive cell density was more intense in the Day 2 group than in the Day 1 and control groups.Discussion: Prevention of apoptosis may potentially improve impaired functions of CVOs after SAH. © W. S. Maney & Son Ltd 2014.

Dergi Adı Neurological Research
Dergi Cilt Bilgisi 36
Dergi Sayısı 12
Sayfalar 1114 - 1120
Yayın Yılı 2014
Eser Adı
[dc.title]
Apoptosis and necrosis in the circumventricular organs after experimental subarachnoid hemorrhage as detected with annexin V and caspase 3 immunostaining
Yazar
[dc.contributor.author]
Edebali N.
Yazar
[dc.contributor.author]
Tekin İ.Ö.
Yazar
[dc.contributor.author]
AçıkgÖz B.
Yazar
[dc.contributor.author]
AçıkgÖz Ş.
Yazar
[dc.contributor.author]
Barut F.
Yazar
[dc.contributor.author]
Sevinç N.
Yazar
[dc.contributor.author]
SümbüloĞlu V.
Yayın Yılı
[dc.date.issued]
2014
Yayıncı
[dc.publisher]
Maney Publishing
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
Objectives: The circumventricular organs (CVOs) are essential for most autonomic and endocrine functions. Trauma and bleeding can affect their function. The aim of this study was to investigate apoptosis and necrosis in CVOs in the early period after experimental subarachnoid hemorrhage (SAH) in rats, using annexin V affinity and caspase 3 immunostaining.Methods: Three experimental groups were used: Days 1 and 2 after SAH, and a control group, seven Wistar albino rats each. Subarachnoid hemorrhage was accomplished by transclival basilar artery puncture. Rats were perfused with 0.9%NaCl and 0.1M phosphate buffer pH 7.4 until heart stoppage. Apoptosis and necrosis in CVOs were measured by flow cytometry with annexin V staining, and by caspase 3 immunostaining.Results: Apoptosis in the organum vasculosum lamina terminalis (OVLT), median eminence (ME), and area postrema (AP) was significantly higher in the Day 1 group than in the control group. Apoptosis in the subfornicial organ (SFO), OVLT, ME, and AP was significantly higher in the Day 2 group than in the control group. There were significant differences between the Day 1 and Day 2 groups, except for AP. Necrosis in SFO and OVLT was significantly higher in the Day 2 group than in the Day 1 or control groups, whereas necrosis in the ME and AP did not differ between the three groups. Caspase 3-positive cell density was more intense in the Day 2 group than in the Day 1 and control groups.Discussion: Prevention of apoptosis may potentially improve impaired functions of CVOs after SAH. © W. S. Maney & Son Ltd 2014.
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Konu Başlıkları
[dc.subject]
Annexin V
Konu Başlıkları
[dc.subject]
Apoptosis
Konu Başlıkları
[dc.subject]
Caspase 3
Konu Başlıkları
[dc.subject]
Circumventricular organs
Konu Başlıkları
[dc.subject]
Subarachnoid hemorrhage
Haklar
[dc.rights]
info:eu-repo/semantics/closedAccess
ISSN
[dc.identifier.issn]
0161-6412
İlk Sayfa Sayısı
[dc.identifier.startpage]
1114
Son Sayfa Sayısı
[dc.identifier.endpage]
1120
Dergi Adı
[dc.relation.journal]
Neurological Research
Dergi Sayısı
[dc.identifier.issue]
12
Dergi Cilt Bilgisi
[dc.identifier.volume]
36
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.1179/1743132814Y.0000000437
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/4327
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Görüntülenme Sayısı ( Zaman Dağılımı )
Görüntülenme
193
09.12.2022 tarihinden bu yana
İndirme
1
09.12.2022 tarihinden bu yana
Son Erişim Tarihi
21 Nisan 2024 04:44
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Tıklayınız
groups control higher necrosis significantly Apoptosis annexin caspase hemorrhage experimental immunostaining apoptosis functions between significant vasculosum lamina terminalis (OVLT) differences median eminence postrema subfornicial whereas except Prevention impaired improve potentially Discussion Necrosis intense density 3-positive
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