Filtreler
Filtreler
Bulunan: 41 Adet 0.106 sn
Ambargo Durumu [1]
Tam Metin [2]
Yayın Türü [3]
Yayın Dili [2]
Konu Başlıkları [90]
Erişime Açık

Effects of some DNA gyrase inhibitors on the survival and development of Pimpla turionellae (Hymenoptera. Ichneumonidae) larvae reared on an artificial diet

Büyükgüzel, Kemal

Endoparazitoid bir zarkanatlı türü, Pimpla turionellae L., kimyasal yapısı bilinen sentetik besin ortamlarında aseptik şartlarda beslenerek, güçlü antibakteriyel ajanlar olan novobiyosin, nalidiksik ve oksolinik asitin böceğin yaşama ve gelişmesine ayrı ayrı etkileri inclendi. Beşinci evredeki larvaların yaşama yüzdesi antibiyotiklerin farklı miktalarını içeren besinler tarafından önemli derecede etkilenmemiştir. Novobiyosinin en düşük miktarını içeren besin pup ve ergin yüzdesini önemli derecede artırmıştır. Bu miktar aynı zamanda beşinci evreye ulaşmak için gereken süreyi kısaltmış, ancak la ...Daha fazlası

Erişime Açık

The effects of boric acid-induced oxidative stress on antioxidant enzymes and survivorship in Galleria mellonella

Hyršl, Pavel | Büyükgüzel, Ender | Büyükgüzel, Kemal

Larvae of the wax moth, Galleria mellonella (L.), were reared from first instar on a diet supplemented with 156, 620, 1,250, or 2,500 ppm boric acid (BA). The content of malondialdehyde (MDA, an oxidative stress indicator), and activities of the antioxidant enzymes [superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), and glutathione peroxidase (GPx)] were determined in the fat body and hemolymph in the 7th instar larvae and newly emerged pupae. Relative to control larvae, MDA was significantly increased in larval hemolymph, larval and pupal fat body, but decreased in t ...Daha fazlası

Erişime Açık

The influence of dietary alpha-solanine on the waxmoth galleria mellonellal

Büyükgüzel, Ender | Büyükgüzel, Kemal | Erdem, Meltem | Adamski, Zbigniew | Adamski, Zbigniew | Marciniak, Pawel | Ziemnicki, Kazimierz

Plant allelochemicals are nonnutritional chemicals that interfere with the biology of herbivores. We posed the hypothesis that ingestion of a glycoalkaloid allelochemical, ?-solanine, impairs biological parameters of greater wax moths Galleria mellonella. To test this idea, we reared wax moths on artificial diets with 0.015, 0.15, or 1.5 mg/100 g diet of ?-solanine. Addition of ?-solanine to the diet affected survival of seventh-instar larvae, pupae, and adults; and female fecundity and fertility. The diet containing the highest ?-solanine concentration led to decreased survivorship, fecundity ...Daha fazlası

Erişime Açık

Effect of boric acid on antioxidant enzyme activity, lipid peroxidation, and ultrastructure of midgut and fat body of Galleria mellonella

Büyükgüzel, Ender | Büyükgüzel, Kemal | Snela, Milena | Erdem, Meltem | Radtke, Katarzyna | Ziemnicki, Kazimierz | Adamski, Zbigniew

Boric acid is widely used as an insecticide, acaricide, herbicide, and fungicide and also during various industrial processings. Hence, numerous populations are subjects to this toxic compound. Its action on animals is still not fully known and understood. We examined the effect of boric acid on larvae of greater wax moth (Galleria mellonella). The chemical appeared to be toxic for larvae, usually in a concentration-dependent manner. Exposed groups revealed increased lipid peroxidation and altered activity of catalase, superoxide dismutase, glutathione S-transferase, and glutathione peroxidase ...Daha fazlası

Erişime Açık

Eicosanoids mediate Galleria mellonella cellular immune response to viral infection

Büyükgüzel, Ender | Tunaz , Hasan | Stanley, David | Büyükgüzel, Kemal

Nodulation is the predominant insect cellular immune response to bacterial and fungal infections and it can also be induced by some viral infections. Treating seventh instar larvae of greater wax moth Galleria mellonella with Bovine herpes simplex virus-1 (BHSV-1) induced nodulation reactions in a dose-dependent manner. Because eicosanoids mediate nodulation reactions to bacterial and fungal infection, we hypothesized that eicosanoids also mediate nodulation reactions to viral challenge. To test this idea, we injected G. mellonella larvae with indomethacin, a nonsteroidal anti-inflammatory dru ...Daha fazlası

Erişime Açık

Prostaglandin biosynthesis by midgut tissue isolated from the tobacco hornworm, Manduca sexta

Büyükgüzel, Kemal | Tunaz, Hasan | Putnam, Sean M. | Stanley, David

We describe prostaglandin (PG) biosynthesis by isolated midgut preparations from tobacco hornworms, Manduca sexta. Microsomal-enriched midgut preparations yielded four PGs, PGA/B2, PGD2, PGE2 and PGF22? all of which were confirmed by analysis on gas chromatography-mass spectrometry (GC-MS). PGA and PGB are double bond isomers which do not resolve on TLC but do resolve by GC; for convenience, we use the single term PGA2 for this product. PGA2 was the major product under most conditions. The midgut preparations were sensitive to reaction conditions, including radioactive substrate, protein conce ...Daha fazlası

Erişime Açık

DNA gyrase inhibitors: Novobiocin enhances the survival of Pimpla turionellae (Hym., Ichneumonidae) larvae reared on an artificial diet but other antibiotics do not

Büyükgüzel, Kemal

Effects of novobiocin, nalidixic and oxolinic acids, which are potent antibacterial agents, on the survival and development of the hymenopterous endoparasitoid, Pimpla turionellae L. (Ichneumonidae) were investigated alone by rearing the larvae aseptically on chemically defined synthetic diets. Survival of the fifth instar larvae was not significantly affected by diets with different levels of antibiotics tested. A diet containing the lowest level of novobiocin significantly increased yields of pupae and adults. This level also significantly shortened the developmental time for fifth instar bu ...Daha fazlası

Erişime Açık

Potato leaf extract and its component, alpha-solanine, exert similar impacts on development and oxidative stress in galleria mellonella L.

Adamski, Zbigniew | Marciniak, Pawel | Ziemnicki, Kazimierz | Büyükgüzel, Ender | Erdem, Meltem | Büyükgüzel, Kemal | Ventrella, Emanuela

Plants synthesize a broad range of secondary metabolites that act as natural defenses against plant pathogens and herbivores. Among these, potato plants produce glycoalkaloids (GAs). In this study, we analyzed the effects of the dried extract of fresh potato leaves (EPL) on the biological parameters of the lepidopteran, Galleria mellonella (L.) and compared its activity to one of the main EPL components, the GA alpha-solanine. Wax moth larvae were reared from first instar on a diet supplemented with three concentrations of EPL or alpha-solanine. Both EPL and alpha-solanine affected survivorshi ...Daha fazlası

Erişime Açık

Effect of eicosanoid biosynthesis inhibitors on the haemolymph protein profile of Galleria mellonella (Linnaeus, 1758) larvae (Lepidoptera: Pyralidae)

Hyrsl, Pavel | Büyükgüzel, Ender | Büyükgüzel, Kemal

Eikosanoidlerin böceklerin hücresel, humoral immün savunma reaksiyonlarına ve strese karşı tepkilerine aracılık ettiği bilinmektedir. Bu aracı moleküllerin işlevi farklı eikosanoid biyosentezi inhibitörleri tarafından spesifik olarak önlenebilir. Bu inhibitörlerin böceklerde total hemolenf protein bileşimi üzerine etkileri detaylı olarak çalışılmamıştır. Eikosanoidlerin böceklerde stress faktörlerine karşı sentezlenen ve diğer savunma reaksiyonlarından sorumlu proteinler üzerinde etkili olarak fizyolojik dengenin sağlanma-sına aracılık ettiği düşünülmüştür. Bu sebeple Büyük bal mumu güvesi Gal ...Daha fazlası

Erişime Açık

Effects of some antimicrobial agents on the total protein content of the endoparasitoid Pimpla turionellae L. (Hymenoptera: Ichneumonidae)

Büyükgüzel, Kemal

Endoparazitoid zarkanatlı türü, Pimpla turionellae L., kimyasal yapısı bilinen sentetik besin ortamlarında aseptik şartlarda beslenerek farklı yapı ve etkiye sahip olan onüç antimikrobiyal ajanın böceğin pup evresindeki total protein miktarına etkileri incelendi. Bu etkiler antimikrobiyal ajanın üeşidine ve besindeki miktarına göre değişmektedir. Pupların total protein miktarı penisillin, streptomisin, rifampisin, tetrasiklin hidroklorür, linkomisin hidroklorür, metil p-hidroksibenzoat, sikloheksimid, ve sodyum benzoat tarafından önemli derecede artırılırken nistatin ise bu miktarı azaltmıştır ...Daha fazlası

Erişime Açık

The effects of oxyclozanide on survival, development and total protein of galleria mellonella L. (Lepidoptera: Pyralidae)

Çelik, Cihat | Büyükgüzel, Kemal | Büyükgüzel, Ender

We investigated that the effects of oxyclozanide on survival rate, development time, adult longevity and the amount of total protein (TP) in different developmental stages (7th-instars larvae, pupae and adult) of greater wax moth Galleria mellonella L. First-instar larvae of insects were reared on 0.003, 0.03 and 0.3 g oxyclozanide in 100 g artificial diets. When compared to all tested concentrations of oxyclozanide and control diet without oxyclozanide, it was confirmed that this anthelmintic drug significantly reduced survival rate of 7th-instar larval stage, pupal and adult stage of G. mell ...Daha fazlası

Oxidative effects of gemifloxacin on some biological traits of drosophila melanogaster (Diptera: Drosophilidae)

Aslan, Nilay | Büyükgüzel, Ender | Büyükgüzel, Kemal

In recent times, several studies have been undertaken on the artificial mass-rearing of insects in which clinical antibacterial antibiotics, including gemifloxacin, which is a DNA gyrase and topoisomerases inhibitor, are amended into the diet to control microbial contamination and preserve diet. The findings of these studies have suggested the possibility that these antibiotics influence the biological traits of insects in relation to their oxidative effects. This study investigated the effects of gemifloxacin on Drosophila melanogaster (Meigen) survival rates, development times, and male-fema ...Daha fazlası

6698 sayılı Kişisel Verilerin Korunması Kanunu kapsamında yükümlülüklerimiz ve çerez politikamız hakkında bilgi sahibi olmak için alttaki bağlantıyı kullanabilirsiniz.
Tamam

creativecommons
Bu site altında yer alan tüm kaynaklar Creative Commons Alıntı-GayriTicari-Türetilemez 4.0 Uluslararası Lisansı ile lisanslanmıştır.
Platforms