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The Use of Thiol/Disulfide as a Novel Marker in Premature Ovarian Failure

Isik, Hatice | Sahbaz, Ahmet | Timur, Hakan | Aynioglu, Oner | Mert, Sule Atalay | Balik, Ahmet Rifat | Erel, Ozcan

Article | 2017 | GYNECOLOGIC AND OBSTETRIC INVESTIGATION82 ( 2 ) , pp.113 - 118

The study aimed to compare the dynamic thiol/disulfide homeostasis between patients with premature ovarian failure (POF) and healthy women. A total of 77 women, 40 POF and 37 healthy controls, were recruited from a university hospital between December 2013 and June 2015. Blood samples were taken from patients to evaluate follicle stimulating hormone (FSH), luteinizing hormone, estradiol and thiol/disulfide levels. A new, fully automated method was used to measure plasma thiol, total thiol and disulfide levels. Disulfide levels, disulfide/native thiol and disulfide/total thiol ratios were significantly increased, native thiol/total t . . .hiol levels were significantly decreased in POF patients compared to controls (p < 0.001). FSH was negatively correlated with native thiol and native thiol/total thiol levels and positively with disulfide, disulfide/native thiol, and disulfide/total thiol levels. This is the first study demonstrating the thiol/disulfide homeostasis in women with POF and may help us understanding the pathophysiology. (C) 2016 S. Karger AG, Base Daha fazlası Daha az

Free oxygen radicals associated with growth in coeliac disease

Ozcetin M. | Katar M. | Yilmaz R. | Karaaslan E. | Ozugurlu F.

Article | 2011 | HealthMED5 ( 5 ) , pp.1008 - 1013

Introduction: Coeliac Disease (CD) is an immune- mediated chronic inflammatory disease of upper small intestine in genetically permanent gluten-sensitive individuals. Oxidative stress was reported to play an important role in the pathogenesis of coeliac disease. The aim of this study was to investigate the frequency of the polimorphisms in the structures of the enzymes SOD and GSHPx with changing levels depending on increased oxidative stress and whether there is an association with the mutations DQA1*0501, DQB1*0201, DRB1*04 reported frequently in coeliac disease. Methods: This study has investigated SOD and GSH-PX polymorphisms an . . .d the frequently reported mutations DQA1*0501, DQB1*0201 and DRB1*04 in the patients with CD. Height and weight measurements of the patients were obtained to evaluate their growth and development, also correlation between polymorphisms SOD and GSH-PX and concerned mutations were investigated. Results: This study involved total 56 cases, 35 female (62.5%) and 21 male (37.5%), with a mean age 6.66 ± 4.18 years. Polymorphisms SOD and GSH-PX were found in homozygote, heterozygote and wild-type patients. At least one of the mutations DQA1*0501, DQB1*0201 and DRB1*04 were found in 41 patients. Conclusion: Although etiology of coeliac disease is not entirely clear, many mechanisms have been suggested. It may be observed that the retardation of growth and development in the patients with coeliac disease may be associated with oxidative stress and decreased antioxidant capacity Daha fazlası Daha az

Pro-inflammatory cytokines and oxidized low-density-lipoprotein in patients with fibromyalgia

Kutu F.C. | Özdolap Ş. | Sarikaya S.

Article | 2019 | Archives of Rheumatology34 ( 2 ) , pp.123 - 129

Objectives: This study aims to evaluate the differences in serum levels of tumor necrosis factor-alpha (TNF-?), interleukin-1 beta (IL-1ß), (IL-8), and oxidized low-density lipoprotein (Ox-LDL) between female fibromyalgia (FM) patients and healthy females and also to examine a possible relationship between the cytokines/biomarker and the severity of pain and clinical features of FM. Patients and methods: This case-control study included 48 female patients (mean age 26.51±6.98 years; range, 18 to 50 years) who were diagnosed with FM according to the American College of Rheumatology criteria and 43 healthy female subjects (mean age 23 . . ..93±4.22 years; range, 18 to 50 years). Serum levels of TNF-?, IL-1ß, IL-8, and Ox-LDL were measured by enzyme-linked immunosorbent assay in both groups. Fibromyalgia Impact Questionnaire, visual analog scale, symptom severity scale, and widespread pain index were carried out and also their relationships with TNF-?, IL-1ß, IL-8, and Ox-LDL levels were evaluated. Results: There was no difference in levels of the serum TNF-?, IL-1ß, IL-8, and Ox-LDL between FM patients and healthy controls (p>0.05). The severity of pain was significantly associated with TNF-? (r=0.338; p=0.021) and IL-8 (r=0.299; p=0.043) levels, and Ox-LDL (r=0.415; p=0.006) level was found to be related to symptom severity of FM. Conclusion: Our results suggest that serum levels of TNF-? and IL-8 may be involved in the occurrence of pain in FM. The level of Ox-LDL may play an important role in the severity of symptoms. Future studies are needed to determine whether and how Ox-LDL plays a role in FM. © 2019 Turkish League Against Rheumatism. All rights reserved Daha fazlası Daha az

Renoprotective effect of Erdosteine in rats against gentamicin nephrotoxicity: A comparison of 99mTc-DMSA uptake with biochemical studies

Cabuk M. | Gurel A. | Sen F. | Demircan N.

Article | 2008 | Molecular and Cellular Biochemistry308 ( 01.Feb ) , pp.35 - 42

Erdosteine is a mucolytic agent having antioxidant properties through its active metabolites in acute injuries induced by pharmacological drugs. This study was designed to investigate the renoprotective potential of Erdosteine against gentamicin (GM)-induced renal dysfunction by using Technetium-99 m dimercaptosuccinic acid (Tc-99 m DMSA) uptake and scintigraphy in rats. For this purpose, male Wistar rats were randomly allotted into one of the four experimental groups: Control, Erdosteine, GM, and GM + Erdosteine groups. GM and GM + Erdosteine groups received 100 mg/kg GM intramuscularly for 6 days. In addition, Erdosteine and GM + . . .Erdosteine groups received 50 mg/kg Erdosteine orally for 6 days. Renal function tests were assessed by serum blood urea nitrogen (BUN), creatinine levels, as well as scintigraphic and tissue radioactivity measurements with Tc-99 m DMSA. Renal oxidative damage was determined by renal malondialdehyde (MDA) levels, by antioxidant enzyme activities; superoxide dismutase (SOD) and catalase (CAT) and activities of oxidant enzymes; xanthine oxidase (XO) and myeloperoxidase (MPO). GM administration resulted in marked renal lipid peroxidation, increased XO and MPO activities and decreased antioxidant enzyme activities. GM + Erdosteine group significantly had lower MDA levels, higher SOD and CAT activities and lower XO and MPO activities, when compared to GM. Also GM + Erdosteine had lower levels of serum BUN, creatinine and higher renal tissue Tc-99 m DMSA uptake and radioactivity with respect to GM. In conclusion, our results supported a protective role of Erdosteine in nephrotoxicity associated with GM treatment. © Springer Science+Business Media, LLC. 2007 Daha fazlası Daha az

Effect of nitric oxide synthase inhibitors in acute lung injury due to blunt lung trauma in rats [Siçanlarda künt akciğer travmasina bağli akut akciğer hasarinda nitrik oksit sentaz inhibitörlerinin etkisi]

Akgül A.G. | Şahin D. | Temel U. | Eliçora A. | Dillioğlugil M. | Kir H.M. | Özsoy Ö.D.

Article | 2019 | Turkish Journal of Thoracic and Cardiovascular Surgery27 ( 1 ) , pp.63 - 72

Background: This study aims to investigate the effects of blunt lung trauma performed in experimental rat model on lung tissue and blood as well as proinflammatory cytokines, oxidant-antioxidant enzymes and histopathological parameters after Ngamma-nitro-L-arginine methyl ester and N-iminoethyl-L-ornithine administration. Methods: The study included 50 adult male Wistar albino rats (weighing 350 to 400 g). Rats were randomly allocated into four groups. Except in the control, moderate-level pulmonary contusion was created in all other groups. Intraperitoneal saline solution was performed in groups 1 and 2, 25 Ngamma-nitro-L-a . . .rginine methyl ester in group 3, and 20 N-iminoethyl-L-ornithine in group 4. Blood and lung tissues were studied biochemically and histopathologically. Results: Best outcomes were recorded statistically significantly in groups with administration of Ngamma-nitro-L-arginine methyl ester and N-iminoethyl-L-ornithine when malondialdehyde response, mucous and histopathological values were examined. Significant improvement was detected in superoxide dismutase values in the group with administration of competitive nitric oxide synthase inhibitor Ngamma-nitro-L-arginine methyl ester. Nitric oxide values were substantially decreased in N-iminoethyl-L-ornithine group, while no significance was detected. Conclusion: Free oxygen radicals and lipid peroxidation played a role in pulmonary contusion after blunt lung trauma. According to biochemical and histopathological outcomes, effects of inflammation were decreased and protective effects were formed with administration of both Ngammanitro- L-arginine methyl ester and N-iminoethyl-L-ornithine. © 2019 Turkish Society of Cardiovascular Surgery.Amaç: Bu çalişmada deneysel siçan modeli üzerinde uygulanan künt akciğer travmasinin akciğer dokusu ve kandaki etkileri ile Ngammanitro- L-arginin metil ester ve N-iminoetil-L-ornitin uygulamasini takiben proenflamatuvar sitokinler, oksidan-antioksidan enzimler ve histopatolojik parametreler araştirildi. çalişma plani: çalişmaya 50 erişkin erkek Wistar albino siçan (ağirlik 350-400 g) dahil edildi. Siçanlar rastgele dört gruba ayrildi. Kontrol dişindaki diğer tüm gruplarda orta şiddette pulmoner kontüzyon oluşturuldu. Grup 1 ve 2'ye intraperitoneal salin solüsyonu, grup 3'e 25 Ngamma-nitro-L-arginin metil ester, grup 4'e 20 N-iminoetil-L-ornitin uygulandi. Kan ve akciğer dokulari biyokimyasal ve histopatolojik açidan incelendi. Bulgular: Malondialdehit yaniti, müköz ve histopatolojik değerler incelendiğinde, en iyi sonuçlar istatistiksel olarak anlamli şekilde Ngamma-nitro-L-arginin metil ester ve N-iminoetil-L-ornitin uygulanan gruplarda kaydedildi. Yarişmali nitrik oksit sentaz inhibitorü Ngamma-nitro-L-arginin metil ester uygulanan grupta süperoksit dismutaz değerlerinde anlamli iyileşme saptandi. Nitrik oksit değerleri N-iminoetil-L-ornitin grubunda oldukça düşük idi ancak anlamlilik saptanmadi. Sonuç: Künt akciğer travmasi sonrasi pulmoner kontüzyonda serbest oksijen radikalleri ve lipid peroksidasyon rol oynadi. Biyokimyasal ve histopatolojik sonuçlara göre, enflamasyon etkileri hem Ngamma-nitro- L-arginin metil ester hem N-iminoetil-L-ornitin uygulamasi ile azaldi ve koruyucu etkiler oluştu. © 2019 Turkish Society of Cardiovascular Surgery Daha fazlası Daha az

Evaluation of oxidative status in short-term exercises of adolescent athletes

Kurkcu R. | Cakmak A. | Zeyrek D. | Atas A. | Karacabey K. | Yamaner F.

Article | 2010 | Biology of Sport27 ( 3 ) , pp.177 - 180

The aim of the study was to evaluate the effects of short-term exercise on total antioxidant status (TAS), lipid hydroperoxide (LOOHs), total oxidative status (TOS) and oxidative stress index (OSI) in adolescent athletes. A total of 62 adolescent participated in the study. Athletes were trained regularly 3 days a week for 2 hours. All subjects followed a circuit exercise program. Blood samples were collected just before and immediately after the exercise program. Antioxidant status was evaluated by measuring the TAS level in the plasma. Oxidative status was evaluated by measuring the total peroxide level. The percentage ratio of TAS . . . to total peroxide level was accepted as the OSI. Plasma triglyceride, total cholesterol, LDL, HDL and VLDL were measured by automated chemical analyzer using commercially available kits.There was a significant increase in TOS ( Daha fazlası Daha az

Acute effects of different doses of malathion on the rat liver

Severcan Ç. | Ekremoglu M. | Sen B. | Pasaoglu O.T. | Akyurek N. | Severcan S.M. | Pasaoglu H.

Article | 2019 | Clinical and Experimental Hepatology5 ( 3 ) , pp.237 - 243

Aim of the study: Our study was designed to evaluate the acute effects of malathion on rat liver tissues. Material and methods: The animals were divided into 4 groups of 6 animals/each. Group 1 (control group) received corn oil, while groups 2, 3, and 4 were given malathion dissolved in corn oil at a dose of 100, 200 and 400 mg/kg, respectively. 24 hours after malathion administration, animals were sacrificed and liver tissues were collected. The liver tissues were then analysed biochemically and histopathologically. Results: Butyrylcholinesterase levels in groups 2, 3 and 4 were significantly lower than that of group 1. Total oxida . . .nt status and tumour necrosis factor alpha level were significantly increased in group 4 compared to group 1. Catalase activities of groups 3 and 4 were significantly higher than that of group 1. Arylesterase activity was significantly decreased in groups 3 and 4 compared to group 1. In groups 3 and 4, some vacuoles in hepatocytes were revealed and hydropic degeneration was observed in group 4. Conclusions: Acute administrations of malathion results in hepatotoxicity in a dose-dependent manner. © 2019 Termedia Publishing House Ltd.. All rights reserved Daha fazlası Daha az

Oxidative stress and protein oxidation in pseudoexfoliation syndrome

Yagci R. | Gürel A. | Ersöz I. | Keskin U.C. | Hepşen I.F. | Duman S. | Yigitoglu R.

Article | 2006 | Current Eye Research31 ( 12 ) , pp.1029 - 1032

Purpose: To investigate the oxidant/antioxidant status and protein oxidation in pseudoexfoliation syndrome. Methods: The activity of serum superoxide dismutase (SOD) and the levels of serum malondialdehyde (MDA) and protein carbonyl (PC) were measured in 50 patients with pseudoexfoliation (PEX) and in 55 healthy controls. Results: There was significant difference in the SOD activity in PEX group compared with the control group (p < 0.001). In addition, MDA and PC levels were significantly higher in patients than in the controls (p < 0.001). Conclusions: Decrease in SOD activity and the higher levels of MDA and PC indicate increased . . .oxidative stress. Our results suggest a possible role of oxidative stress in pathology of PEX syndrome. Copyright © Informa Healthcare Daha fazlası Daha az

Melatonin treatment against remote organ injury induced by renal ischemia reperfusion injury in diabetes mellitus

Fadillioglu E. | Kurcer Z. | Parlakpinar H. | Iraz M. | Gursul C.

Article | 2008 | Archives of Pharmacal Research31 ( 6 ) , pp.705 - 712

Oxidative stress may have a role in liver damage after acute renal injury due to various reasons such as ischemia reperfusion (IR). Diabetes mellitus (DM) is an important disease for kidneys and may cause nephropathy as a long term complication. The aim of this study was to investigate protective effect of melatonin, a potent antioxidant, against distant organ injury on liver induced by renal IR in rats with or without DM. The rats were divided into six groups: control (n=7), DM (n=5), IR (n=7), DM+IR (n=7), melatonin+IR (Mel+IR) (melatonin, 4 mg/ kg during 15 days) (n=7), and Mel+DM+IR groups (n=7). Diabetes developed 3 days after . . .single i.p. dose of 45 mg/kg streptozotocin. After 15 day, the left renal artery was occluded for 30 min followed 24 h of reperfusion in IR performed groups. DM did not alter oxidative parameters alone in liver tissue. The levels of malondialdehyde, protein carbonyl and nitric oxide with activities of xanthine oxidase and myeloperoxidase were increased in liver tissues of diabetic and non-diabetic IR groups. Nitric oxide level in DM was higher than control. The activities of catalase and superoxide dismutase were increased in IR groups in comparison with control and DM. ALT and AST levels were higher in IR and DM+IR groups than control and DM. Melatonin treatment reversed all these oxidant and antioxidant parameters to control values as well as serum liver enzymes. We concluded that renal IR may affect distant organs such as liver and oxidative stress may play role on this injury, but DM has not an effect on kidney induced distant organ injury via oxidant stress. Also, it was concluded that melatonin treatment may prevent liver oxidant stress induced by distant injury of kidney IR. © 2008 The Pharmaceutical Society of Korea Daha fazlası Daha az

Comparative study of oxidative stress in maternal blood with that of cord blood and maternal milk

Erdem M. | Harma M. | Harma I.M. | Arikan I. | Barut A.

Article | 2012 | Archives of Gynecology and Obstetrics285 ( 2 ) , pp.371 - 375

Objective Oxidative stress has been implicated in pregnancy-induced hypertension and preeclampsia. There is still some debate over whether this is confined to the placenta or occurs in the maternal circulation. This study was designed to investigate this question by comparing parameters of oxidative stress in samples of maternal blood and cord blood taken from normotensive and hypertensive pregnant women. A further aim was to compare these parameters in maternal milk from the two populations. Study design Forty-six hypertensive (31 preeclamptic and 15 chronic hypertensive) and 60 normotensive pregnant women were recruited. Antecubit . . .al blood was collected from each woman before and after delivery, cord blood just after delivery, and maternal milk for the first 3 days post-partum. Total antioxidant capacity, total peroxides and sulphydryl concentration were measured and total antiox-idant capacity calculated for each specimen. Results For all specimens (maternal blood plasma, cord blood plasma, maternal milk) total antioxidant capacity and sulphydryl concentration were significantly lower in the hypertensive women than in the normotensive, while total peroxide and oxidative stress index were significantly higher. Conclusion These results support the association between hypertension in pregnancy and oxidative stress and the view that this occurs throughout the maternal circulation. They show further that oxidative stress may be transmitted from mother to newborn in maternal milk. Supplementation with antioxidants could, in view of our findings, possibly provide protection to the mother and fetus and also, through transmission in maternal milk, to the newborn. © 2011 Springer-Verlag Daha fazlası Daha az

Evaluation of preventive effect of shilajit on radiation-induced apoptosis on ovaries

Kececi M. | Akpolat M. | Gulle K. | Gencer E. | Sahbaz A.

Article | 2016 | Archives of Gynecology and Obstetrics293 ( 6 ) , pp.1255 - 1262

Purpose: Cancer is the second leading cause of death in children in developed countries and most of childhood malignancies can be treated with chemo-radiotherapy. Although radiation therapy is a successful treatment modality in cancer patients, it has various adverse effects. Especially the gonads are very sensitive and prone to radiation-related damage. Radiation impairs the ovaries by triggering apoptosis of follicular cells and chromosomal damage and oxidative stress. Shilajit, a traditional medicinal agent in India, Russia, and other parts of the world, contains various antioxidant agents and has ovogenic effects. To evaluate th . . .e ability of shilajit to prevent radiation-induced ovarian damage. Methods: Forty Wistar albino female rats were divided into four groups as: Control group, shilajit group, radiation only group, and radiation + shilajit group. Four days after radiation exposure, the rats were sacrificed and the ovaries were removed and evaluated immuno-histopathologically. Results: There was a statistically significant difference in follicle counts (primordial, primary, preantral, antral, and atretic follicles) between the groups (p Daha fazlası Daha az

Agmatine attenuates intestinal ischemia and reperfusion injury by reducing oxidative stress and inflammatory reaction in rats

Turan, İnci | Özaçmak-Sayan, Hale | Özaçmak, Veysel Haktan | Barut, Figen | Araslı, Mehmet

Article | 2017 | Life Sciences189 , pp.23 - 28

Aims Oxidative stress and inflammatory response are major factors causing several tissue injuries in intestinal ischemia and reperfusion (I/R). Agmatine has been reported to attenuate I/R injury of various organs. The present study aims to analyze the possible protective effects of agmatine on intestinal I/R injury in rats. Main methods Four groups were designed: sham control, agmatine-treated control, I/R control, and agmatine-treated I/R groups. IR injury of small intestine was induced by the occlusion of the superior mesenteric artery for half an hour to be followed by a 3-hour-long reperfusion. Agmatine (10 mg/kg) was administer . . .ed intraperitoneally before reperfusion period. After 180 min of reperfusion period, the contractile responses to both carbachol and potassium chloride (KCl) were subsequently examined in an isolated-organ bath. Malondialdehyde (MDA), reduced glutathione (GSH), and the activity of myeloperoxidase (MPO) were measured in intestinal tissue. Plasma cytokine levels were determined. The expression of the intestinal inducible nitric oxide synthase (iNOS) was also assessed by immunohistochemistry. Key findings The treatment with agmatine appeared to be significantly effective in reducing the MDA content and MPO activity besides restoring the content of GSH. The treatment also attenuated the histological injury. The increases in the I/R induced expressions of iNOS, IFN-?, and IL-1? were brought back to the sham control levels by the treatment as well. Significance Our findings indicate that the agmatine pretreatment may ameliorate reperfusion induced injury in small intestine mainly due to reducing inflammatory response and oxidative stress. © 2017 Elsevier Inc Daha fazlası Daha az

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