Korkut, Şeyda | Kılıç, Muhammet Samet | Hazer, Baki

Article | 2019 | Asia-Pacific Journal of Chemical Engineering14 ( 6 )

A copolymer poly(methyl methacrylate-co-vinylferrocene) was synthesized and used for the first time in a biofuel cell design. Bioanaode enzyme glucose oxidase and biocathode enzyme bilirubin oxidase were physically immobilized onto the copolymer-modified electrodes. Characterization studies were conducted by scanning electron microscopy, carbon-13, fourier transform infrared and hydrogen-1 nuclear magnetic resonance, and cyclic voltammograms. The designed biofuel cell was operated with linear sweep voltammetry. The maximum current was at 45°C with 120 µg of polymer amount. An improved power density of 323 µW cm-2 that is higher than . . . other ferrocene-based fuel cells was obtained with 10-mM glucose at 0.4 V with the designed bioanode. © 2019 John Wiley & Sons Ltd Daha fazlası Daha az

Kılıç, Muhammet Samet | Korkut, Şeyda | Hazer, Baki

Article | 2017 | Electroanalysis29 ( 10 ) , pp.2377 - 2384

Newly synthesized poly(styrene-g-oleic amide) was coated onto a rhodium nanoparticle modified glassy carbon (GC) surface for the fabrication of horseradish peroxidase based biosensor used for hydrogen peroxide detection. The rhodium modifed electrode presented ten times higher signal than unmodified electrode even at low elecrtroactive enzyme quantity by enhancing the electron transfer rate at the applied potential of -0.65 V. The biosensor designed by under the optimized rhodium electrodeposition time exhibited a fast response less than 5 s, an excellent operational stability with a relative standard deviation of 0.6 % (n=6), an ac . . .curacy of 96 % and a large linear range between 50 µM and 120 mM for hydrogen peroxide. Detection limit and the sensitivity parameters were calculated to be 44 µM and 57 µA mM-1 cm-2, respectively by preserving its entire initial response up to the 15 days, while only 20 % of its initial response was lost at the end of one month. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinhei Daha fazlası Daha az

Çabuk, Hasan | Kılıç, Muhammet Samet | Ören, Muhammet

Article | 2014 | Environmental Monitoring and Assessment186 ( 3 ) , pp.1515 - 1524

This research was carried out in the cities of Zonguldak and Eregli, which have been characterized as urban and industrial environments of the Western Black Sea Region, Turkey, in order to assess the contamination of polycyclic aromatic hydrocarbons (PAHs) using mosses as biomonitors. The methodology involved the collection of moss samples (Hypnum cupressiforme), ultrasonic extraction with dichloromethane, cleanup using silica gel and analysis by liquid chromatography with ultraviolet detection. The total PAH concentrations ranged from 78.1 to 1693.5 ng g -1 in Zonguldak and from 15.2 to 275.1 ng g -1 in Eregli. The total PAH concen . . .tration in Eregli was about six times lower than that in Zonguldak, revealing the importance of switching from coal to natural gas in residential heating. The diagnostic ratios and the correlation analysis have indicated that coal combustion and traffic emissions were the major PAH sources at both sites. The contour maps were constructed for the determination of spatial distributions of total PAHs, and it was shown for Zonguldak as well as for Eregli that the PAH pollution was much more predominant in highly populated regions. Moving away from the city centres, a gradual decrease in PAH pollution rates was observed. © 2013 Springer Science+Business Media Daha fazlası Daha az

Korkut, Şeyda | Kılıç, Muhammet Samet | Uzunçar, Sinan | Hazer, Baki

Article | 2016 | Analytical Letters49 ( 14 ) , pp.2322 - 2336

A carboxylated poly(styrene-b-isoprene-b-styrene) triblock copolymer was synthesized for the construction of an enzymatic fuel cell. Glucose oxidase and bilirubin oxidase were chemically immobilized via the carboxylated functional groups of the polymer. The enzymatic fuel cell working electrodes were modified with graphene to accelerate the electron transfer rate of the system. Essential design and operational parameters were carefully optimized for improving the power of the enzymatic fuel cell. A power density of 20 µW cm-2 with only 4 µg of immobilized bilirubin oxidase was generated from 30 mM glucose at 0.72 V. The improved enz . . .ymatic fuel cell was tested in a plant leaf. A power density of 14 nW cm-2 was generated with glucose produced by photosynthesis reactions conducted during 30 min in the leaf. © 2016, Taylor & Francis Group, LLC Daha fazlası Daha az

Korkut, Şeyda | Uzunçar, Sinan | Kılıç, Muhammet Samet | Hazer, Baki

Article | 2016 | Instrumentation Science and Technology44 ( 6 ) , pp.614 - 628

A novel continuous flow biosensor based on gold nanoparticles and poly(propylene-co-imidazole) was developed for the online determination of p-benzoquinone. The amperometric response was measured as a function of p-benzoquinone concentration at an applied potential of -50 mV. The hydrogen peroxide concentration was optimized and fixed at 1 mM in samples. The mass transfer resistance of the copolymer film was minimized, and the flow cell was regenerated quickly at 1 mL/min. The resulting device provided good analytical performance based on a linear dynamic range from 5–100 µM, a short response time of 3 s, a detection limit of 3.3 µM . . ., excellent repeatability with a relative standard deviation of 0.82%, long-term stability of 95% after four weeks, and an accuracy of 105%. The gold nanoparticles enhanced the electron transfer rate on the electrode. The apparent Michaelis-Menten constant was 4 mM, showing that the enzyme retained catalytic specificity and provided high activity for p-benzoquinone. © 2016, Taylor & Francis Daha fazlası Daha az

Korkut, Şeyda | Uzunçar, Sinan | Kılıç, Muhammet Samet | Hazer, Baki

Article | 2019 | Instrumentation Science and Technology47 ( 1 ) , pp.1 - 18

A new amperometric urea biosensor based on gold nanoparticle embedded poly(propylene-co-imidazole) was developed for the determination of urea. The urease adsorbed on the polymeric film catalyzed the hydrolysis of urea to ammonium and bicarbonate ions and the ammonium was then electrooxidized on the gold electrode with the aid of gold nanoparticles at +0.2 V versus Ag/AgCl using differential pulse voltammetry. The biosensor provided a linear current response to urea concentration from 0.1 to 30 mM, a detection limit of 36 µM, a relative standard deviation of 2.43% (n = 18), and excellent storage stability, as the current decrease wa . . .s only 3% after 75 days. The operation of the biosensor was evaluated by the analysis of municipal sewage wastewater collected from the inlet pipe of the treatment plant of Zonguldak City in Turkey. The effects of possible interferants were also characterized. © 2018, © 2018 Taylor & Francis Daha fazlası Daha az

Kılıç, Muhammet Samet | Korkut, Şeyda | Hazer, Baki

Article | 2015 | Materials Science and Engineering C47 , pp.165 - 171

This study describes the construction of an enzymatic fuel cell comprised of novel gold nanoparticles embedded poly(propylene-co-imidazole) coated anode and cathode. Working electrode fabrication steps and operational conditions for the fuel cell have been optimized to get enhanced power output. Electrical generation capacity of the optimized cell was tested by using the municipal wastewater sample. The enzymatic fuel cell system reached to maximum power density with 1 µg and 8 µg of polymer quantity and bilirubin oxidase on electrode surface, respectively. The maximum power output was calculated to be 5 µW cm- 2 at + 0.56 V (vs. Ag . . ./AgCl) in phosphate buffer (pH 7.4, 100 mM, 20 °C) by the addition of 15 mM of glucose as a fuel source. The optimized enzymatic fuel cell generated a power density of 0.46 µW cm- 2 for the municipal wastewater sample. Poly(propylene-co-imidazole) was easily used for a fuel cell system owing to its metallic nanoparticle content. The developed fuel cell will play a significant role for energy conversion by using glucose readily found in wastewater and in vivo mediums. © 2014 Elsevier B.V. All rights reserved Daha fazlası Daha az

Korkut, Şeyda | Kılıç, Muhammet Samet | Sanal, Timur | Hazer, Baki

Article | 2017 | Materials Science and Engineering C76 , pp.787 - 793

This study describes construction of an enzymatic fuel cell comprised of poly(caprolactone-g-ethylene glycol) coated novel glucose oxidase anode and laccase cathode. Rationally designed poly(caprolactone-g-ethylene glycol) containing various poly(ethylene glycol) percentages ranging between 2.67 and 15.04% were synthesized chemically and tested separately for operation of the fuel cell system to achieve the best energy generation. The maximum power density was found to be 80.55 µW cm- 2 at 0.91 V (vs. Ag/AgCl) in pH 5, 100 mM citrate buffer (20 °C) by the addition of 30 mM of glucose from the electrodes coated with 11.34% poly(ethyl . . .ene glycol) containing polymer with a quantity of 600 µg. High poly(ethylene glycol) percentages with more numbers of long poly(ethylene glycol) brushes lead to the creation of a complexity in the polymer morphology and steric hindrance effect for electron transport. The graft copolymer was easily used for the fuel cell system owing to its biocompatible and microporous film morphology. The grafted polymer was able to facilitate enzymatic glucose oxidation and oxygen reduction while simultaneously producing high catalytic electrical currents. © 2017 Elsevier B.V Daha fazlası Daha az

Kılıç, Muhammet Samet | Korkut, Şeyda | Hazer, Baki

Article | 2014 | WIT Transactions on Ecology and the Environment181 , pp.213 - 224

Enzymatic biofuel cells (EFCs), which employ enzymes as a catalyst, convert the chemical energy released from the enzymatic oxidation of fuel into electrical energy. While chemical energy is being generated by the oxidation of fuel with enzymes, electricity is generated simultaneously by the movement of electrons released as a consequence of this chemical reaction from anode through cathode in enzymatic fuel cells. The major problem encountered in EFC studies is the difficult/slow electron transfer between the enzyme and the electrode. To mediate the electron transfer between the enzyme and the electrode's surface, low molecular wei . . .ght redox compounds called mediators are used in EFC. Higher power generation and minimal interference effects at a lower cell potential are achieved by using a mediator in EFC. The scope of this study is for the development of an enhanced electron transferred EFC with a proper mediator for the generation of electrical energy by the oxidation of glucose in domestic wastewater. Therefore, glucose in domestic wastewater is utilised for energy generation. In this study, Polypyrrole-2-carboxyclic acid was modified with various mediators such as, ferrocene, neutral red and p-benzoquinone, which were tested for this purpose. The maximum power density (100 nW/cm2) was observed for the ferrocene modified electrodes including glucose oxidase and laccase as anodic and cathodic enzyme, respectively. The electrode fabrication step was optimized with respect to the electrode material, its operational potential and the thickness of the polymeric film. The highest current values were obtained from the addition of 10 mM of glucose for the EFC system designed with the gold electrode material and operated with a working potential of 0.45 V. The most suitable polymeric film thickness was achieved in the cyclic voltammetry parameters set up with a scan rate of 50 mV/s and 25 cycles. The optimized EFC was tested in the domestic wastewater of Zonguldak City in Turkey. Ferrocene mediated EFC yielded a power density of 50-200 nW/cm2 for the domestic wastewater. © 2014 WIT Press Daha fazlası Daha az

Korkut, Şeyda | Kılıç, Muhammet Samet

Article | 2016 | Environmental Progress and Sustainable Energy35 ( 3 ) , pp.859 - 866

Poly(3-thiopheneacetic acid-co-3-methylthiophene) conductive polymer was electrosynthesized with ferrocene and used for an enzymatic fuel cell including glucose oxidase and bilirubin oxidase enzymes. The system was operated in a single-compartment and membrane-less cell by using glucose as fuel. Detailed optimization ensured to achieve considerable power output to generate sustainable energy from municipal wastewater as a renewable fuel source. Maximum power density of 1 µW/cm2was generated at a cell voltage of +0.56 V in 100 mM, pH 7.4 phosphate buffer with the addition of 10 mM synthetic glucose. The working electrodes could harve . . .st glucose readily found in the municipal wastewater of Zonguldak City in Turkey by generating a power density of 4 µW/cm2for the municipal wastewater sample. In this way, the organic pollutants in wastewater could be evaluated by converting them into the electrical energy using an enzymatic fuel cell for the first time. © 2015 American Institute of Chemical Engineers Environ Prog, 35: 859–866, 2016. © 2015 American Institute of Chemical Engineers Environ Pro Daha fazlası Daha az

Korkut, Şeyda | Kılıç, Muhammet Samet

Article | 2016 | Environmental Technology (United Kingdom)37 ( 2 ) , pp.163 - 171

The present work reported a compartment-less enzymatic fuel cell (EFC) based on newly synthesized Poly(pyrrole-2-carboxylic acid-co-3-thiophene acetic acid) film containing glucose oxidase and laccase effectively wired by p-benzoquinone incorporated into the copolymer structure. The resulting system generated a power density of 18.8 µW/cm2 with 30 mM of glucose addition at +0.94 V at room temperature. Improvements to maximize the power output were ensured with step-by-step optimization of electrode fabrication design and operational parameters for operating the system with renewable fuel sources. We demonstrated that the improved fu . . .el cell could easily harvest glucose produced during photosynthesis to produce electrical energy in a simple, renewable and sustainable way by generating a power density of 10 nW/cm2 in the plant leaf within 2 min. An EFC for the first time was successfully operated in municipal wastewater which contained glycolytic substances to generate electrical energy with a power output of 3.3 µW/cm2. © 2015 Taylor & Francis Daha fazlası Daha az

Kılıç, Muhammet Samet

Article | 2017 | Instrumentation Science and Technology45 ( 3 ) , pp.276 - 289

Poly[1-(2-aminophenyl) pyrrole] film was electropolymerized on a gold electrode and used for the first time as the matrix for the electrochemical determination of glucose. The working electrode where glucose oxidase was chemically immobilized by amine groups of the poly[1-(2-aminophenyl) pyrrole] film was used for online glucose determination with flow-injection operation. The optimum conditions for glucose were a flow rate of 4 mL/min at +0.7 V in 100 mM using pH 6.5 phosphate buffer as the carrier. The electrode provided a long linear dynamic range between 0.2 mM and 30 mM, a response time of 9 s, a detection limit of 63 µM, a rel . . .ative standard deviation of 0.7%, and an accuracy of 99.95%. The working electrode preserved 64% of its initial current value following six weeks. The optimized device was employed to determine glucose in wastewater from the Zonguldak Biological Treatment Plant, Turkey. © 2017, Copyright © Taylor & Francis Group, LLC Daha fazlası Daha az