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Seed coat ultrastructure of hard-seeded and soft-seeded varieties of Vicia sativa

Büyükkartal H.N. | Çölgeçen H. | Pinar N.M. | Erdogan N.

Makale | 2013 | Turkish Journal of Botany37 ( 2 ) , pp.270 - 275

The mature seed coat structure in hard-seeded and soft-seeded varieties of Vicia sativa L. was examined by scanning and transmission electron microscopy to investigate differentiation in cytological and morphological features between these varieties. The outer layer of the seed coats was composed of macrosclereid (Malpighian) cells in the hard-seeded and soft-seeded varieties of Vicia sativa. The walls of the macrosclereid cells were thickened, and the cell vacuoles were filled with tannin. The cytoplasm of the macrosclereids contained small- and large-sized vacuoles and ribosomes, mitochondria, and endoplasmic reticulum. Under this . . . layer there was a hypodermal layer composed of osteosclereids separated by large intercellular spaces. The bottom layer consisted of parenchyma cells. Thin-walled parenchyma cells were filled with amyloplasts that contained large starch grains, protein bodies, and a few organelles. Seed size and colour are important characteristics for distinguishing between hard-seeded and soft-seeded varieties. The hard seeds of Vicia sativa are smaller than the soft seeds. © TÜBİTAK Daha fazlası Daha az

The moss flora of Ankara-Kızılcahamam Soguksu National Park

Uyar, Güray | Çetin, Barbaros

Diğer | 2001 | Turkish Journal of Botany25 ( 5 ) , pp.261 - 273

In the Ankara-Kızılcahamam Soğuksu National Park,116 taxa of bryophytes were identified by the authors. These belong to 20 families and 42 genera of bryophytes. Of these, 32 taxa are new for the A2 grid-square in the system adopted by Henderson. In the Ankara-Kızılcahamam Soğuksu National Park,116 taxa of bryophytes were identified by the authors. These belong to 20 families and 42 genera of bryophytes. Of these, 32 taxa are new for the A2 grid-square in the system adopted by Henderson.

Effects of salt stress and synthetic hormone polystimuline K on photosynhetic activity of Trianea bogotensis Karst

Allahverdıev, Surhay Ragimoğlu | Mavituna, Meltem | Ganieva, Rena | Nafısı, Saeedeh

Makale | 1998 | Turkish Journal of Botany22 ( 1 ) , pp.19 - 23

Bu çalışmada kinetin benzeri sentetik hormon olan Polystimulin K (PS-K)'nin tuz stresi uygulanmış Trianea bogotensis Karst'ın (Hydrocharitaceae) fotosentetik aktivitesi üzerine etkisi gösterildi. Değişik zaman sürelerinde (15, 30, 60, 360 ve 720 dakika) farklı tuz stresi (103, 155 ve 344 mM) uygulanmaları sonucunda , Fotosistem II (FS ll)'nin hassasiyetini incelemek için Klorofil değişken Floresans (Chlorophyll variable fluorescence) (Fv) ve yavaş floresans (Slow Fluorescence) (SF) kullanıldı. Fideler üzerindeki tuz (155 mM NaCl) uygulaması SF boyutunu yaklaşık % 50 engelledi. Fidelerin 30 dakika süreyle 20 mg/l PS-K uygulamasına ta . . .bi tutulması ve daha sonra tuz ortamına alınması sonucunda SF boyutunun yükselmesi saptanmıştır. Tuz stresi altında, bütün zaman süreçlerinde, Fv/Fo oranı azalma göstermektedir. Uygulamaların 60 dakika'sına kadar NaCl ve PS-K'nin bulunduğu ortamda Fv/Fo miktarı bir düşüş gösterip, daha sonra hafif bir yükseliş tespit edildi. Bu çalışmanın sonuçları PS-K'nin tuz stresi mekanizmasındaki koruyucu görevini anlamak açısından önemlidir. In the present study the effect of kinetin like synthetic hormone Polystimuline K (PS-K) on photosynthetic activity of salt stressed Trianea bogotensis Karst (Hydrocharitaceae) has been shown. Chlorophyll variable fluorescence (Fv) and slow fluo¬rescence (SF) have been used to investigate the sensitivity of Photosystem 11 (PS II) to different salt stress treatments (103, 155 and 344 mM), at different time courses (15, 30, 60, 360 and 720 minutes). Salt stress (155 mM NaCl) treatments of the seed¬lings inhibited the SF magnitude approximately to 50 %. Pretreatment of seedlings for 30 minutes in 20 mg/l PS-K followed by salt stress resulted in an increase in SF magnitude. Under salt stress the magnitude of Fv/Fo decreases within all time measurments. In the presence of NaCl and PS-K until 60 min. of treatment there was a decrease in Fv/Fo value, then a slight increase was ob¬served. The results of this study are important in understanding the protective function of PS-K on salt stress mechanism Daha fazlası Daha az

Pollen morphology of Astragalus L. sect. Onobrychoidei DC. (Fabaceae) in Turkey

Pınar, Nur M. | Ekici, Murat | Aytaç, Zeki | Akan, Hasan | Çeter, Talip | Alan, Şenol

Makale | 2009 | Turkish Journal of Botany33 ( 4 ) , pp.291 - 303

Türkiye’de yayılış gösteren Astragalus L. cinsinin Onobrychoidei DC. seksiyonunda yer alan 29 türe ait 45 örneğin polen morfolojisi ışık ve elektron mikroskobuyla çalışılmıştır. Polenler genellikle trikolporat aperture sahip, prolat, subprolat veya prolat-sferiodaldir. Polenlerin polar eksenleri 23,4-42,6 ?m ve ekvatoral eksenleri 14,3-36,4 ?m arasında değişmektedir. Dış görünümler meridional optik bölgede eliptik veya basık oval, polar optik bölgede ise trilobulat bazen tetralobulattır. Polen yüzeyi süslenmesi meridional optik bölgede genellikle mikroretikülat, nadiren retikülat veya rugulat, polar optik bölgede ise psilat ve perfo . . .rattır. The pollen morphology of 45 specimens of 29 species of the genus Astragalus L. of the section Onobrychoidei DC. distributed in Turkey was studied with light and electron microscopies. The pollen is generally 3-colporate. Pollen of the section is prolate, subprolate, and prolate-spheroidal. Size varies, with the polar axis ranging from 23.4 to 42.6 μm and the equatorial axis from 14.3 to 36.4 μm. The outline is elliptic or compressed oval in the meridional section, versus trilobulate and sometimes tetralobulate in the polar optical section. Sculpturing is usually microreticulate, rarely reticulate or rugulate in the meridional optical section, and psilate and perforate (with irregular or circular perforations) in the polar optical section Daha fazlası Daha az

Effect of salt stress and synthetic hormone polystimuline K on the photosynthetic activity of cotton (Gossypium hirsutum)

Ganıeva, Rena A. | Allahverdıyev, Surhay R. | Guseınova, Nilufar B. | Kavaklı, Halil I. | Nafisi, Saeedeh

Makale | 1998 | Turkish Journal of Botany22 ( 4 ) , pp.217 - 221

Bir sitokinin anaoğlu olan Polistimülin’nin K (PS-K) farklı periotlarda tuz stresine (155 mM NaCl) maruz kalmış farklı pamuk (Gossypium hirsutum L.) genotiplerindeki gecikmiş ışık komponent durumu (DF), aralıklı flöresans (IF) ve klorofil (Chl a/b) oranı üzerindeki etkisi araştırıldı. Tuz stresinin erken aşamalarında, DF ve IF dalga boylarında düşmeye neden oldugu ve bunun fotosistem II (PS II) aktivitesinin azalması yoluyla ortaya çıktığı gösterilmiştir. Bu düşüş, alıcı PS II merkezindeki klorofil b moleküllerinin azalması ve bunun sonucu olarak Chl a/b oranının yükselmesiyle oluşmaktadır. Buna karsın, etkiye maruz kalan degiskenle . . .rin PS-K ön-muamelesi sonucu düzelmesi, bu sentetik büyüme düzenleyicisinin tiylakoid membranları üzerinde kararlılık kazandırıcı etkisi oldugunu ortaya koymuştur. Bu kararlılık, öncelikle H+/NaCl degisiminin normalize edilmesi ve böylece zarara ugramıs PS II merkezinin onarımının hızlandırılmasına neden olmaktadır. The effects of Polystimuline K (PS-K), as a cytokinin analogue, on delayed light component state (DF), intermittent fluorescence (IF) and chlorophyll (Chl) a/b ratio was investigated in the leaves of different genotypes of cotton (Gossypium hirsutum L.) under salt stress (155 mM Na Cl) for different periods of time. It was shown that in the early perioıd of salt stress, DF and IF amplitudes are decreased, which is indicative of a Photosystem II (PS II) activity decrease. It is possibly related to the damage of chlorophyll in the PS II donor site and the decrease of Chl b molecules leading to an increased Chl a/b ratio. However, the improvement of the affected variables as a consequence of PS-K pretreatment suggests the stablizing effects of the synthetic growth regulator on the thylakoid membranes leading to a normalization of H+/NaCl exchange that consequently hastens the recovery of damaged PS II centers Daha fazlası Daha az

Seed coat ultrastructure of hard-seeded and soft-seeded varieties of Vicia sativa

Büyükkartal, Hatice Nurhan | Çölgeçen, Hatice | Pınar, Nur Münevver | Erdoğan, Neslihan

Makale | 2013 | Turkish Journal of Botany37 ( 2 ) , pp.270 - 275

The mature seed coat structure in hard-seeded and soft-seeded varieties of Vicia sativa L. was examined by scanning and transmission electron microscopy to investigate differentiation in cytological and morphological features between these varieties. The outer layer of the seed coats was composed of macrosclereid (Malpighian) cells in the hard-seeded and soft-seeded varieties of Vicia sativa. The walls of the macrosclereid cells were thickened, and the cell vacuoles were filled with tannin. The cytoplasm of the macrosclereids contained small- and large-sized vacuoles and ribosomes, mitochondria, and endoplasmic reticulum. Under this . . . layer there was a hypodermal layer composed of osteosclereids separated by large intercellular spaces. The bottom layer consisted of parenchyma cells. Thin-walled parenchyma cells were filled with amyloplasts that contained large starch grains, protein bodies, and a few organelles. Seed size and colour are important characteristics for distinguishing between hard-seeded and soft-seeded varieties. The hard seeds of Vicia sativa are smaller than the soft seeds. The mature seed coat structure in hard-seeded and soft-seeded varieties of Vicia sativa L. was examined by scanning and transmission electron microscopy to investigate differentiation in cytological and morphological features between these varieties. The outer layer of the seed coats was composed of macrosclereid (Malpighian) cells in the hard-seeded and soft-seeded varieties of Vicia sativa. The walls of the macrosclereid cells were thickened, and the cell vacuoles were filled with tannin. The cytoplasm of the macrosclereids contained small- and large-sized vacuoles and ribosomes, mitochondria, and endoplasmic reticulum. Under this layer there was a hypodermal layer composed of osteosclereids separated by large intercellular spaces. The bottom layer consisted of parenchyma cells. Thin-walled parenchyma cells were filled with amyloplasts that contained large starch grains, protein bodies, and a few organelles. Seed size and colour are important characteristics for distinguishing between hard-seeded and soft-seeded varieties. The hard seeds of Vicia sativa are smaller than the soft seeds Daha fazlası Daha az

A new record for the flora of Turkey: Scorzonera ketzkhovelii Grossh. (Asteraceae)

Hamzaoğlu, Ergin | Aksoy, Ahmet | Martin, Esra | Pınar, Nur Münevver | Çölgeçen, Hatice

Makale | 2010 | Turkish Journal of Botany34 ( 1 ) , pp.57 - 61

Yusufeli, Artvin’den (kuzeydoğu Anadolu) toplanan Scorzonera ketzkhovelii Grossh. (Asteraceae) Türkiye florası için yeni bir kayıttır. Tür betimlendi ve resmedildi. Ayrıca, türün karyolojik özellikleri ve polen morfolojisi verildi. Scorzonera ketzkhovelii Grossh. (Asteraceae) was recently collected from Yusufeli, Artvin (north-east Anatolia) and it has been reported as a new species record for the flora of Turkey. It is described and illustrated. In addition, its karyological features and pollen morphology are given.

Ultrastructural observations in somatic embryogenesis ofnatural tetraploid Trifolium pratense L.

Çölgeçen, Hatice | Büyükkartal, Hatice Nurhan | Çalışkan, Ufuk Koca

Makale | 2016 | Turkish Journal of Botany40 ( 5 ) , pp.496 - 505

Previous reports of plant regeneration of natural tetraploid T. pratense L.‘Elçi’could be realized only through the apical meristem calli. In order to proceed to the production stage, other regeneration methods need to be tried. Aseptic seedlings were used for the production of somatic embryos through various 2,4-D and kinetin trials. Nonuniform external callus cells with translucent cytoplasm were observed in various developmental stages of somatic embryos. Beneath these cells, there were uniformly aligned, dark-stained embryo cells with dense cytoplasm. Despite the similar developmental stages and cell characteristics of zy . . .gotic and somatic embryos, the walls of somatic embryo cells revealed a highly wavy pattern. The nucleus generally contained only one nucleolus, which was spherical, dark stained, and electron-dense. Electron-dense droplets were seen in vacuoles. The cytoplasm consisted of starch-containing amyloplasts, mitochondria, plastids, ribosomes, endoplasmic reticulum, dictyosomes, lipid, and protein bodies. In some of the somatic embryos at the globular and heart stages, vacuole or electron-translucent zones were observed in the nucleolus. Additionally, a few embryo degenerations were recorded during developmental stages of the zygotic embryo. For the first time, the somatic embryos of natural tetraploid T. pratense were produced from hypocotyl (85%), cotyledon (75%), and apical meristem (60%) explants in 0.3 mg/L 2,4-D and 2 mg/L kinetin-containing MS medium. Our study developed an effective and efficient in vitro production method for using natural tetraploid T. pratense in biotechnological studies. Previous reports of plant regeneration of natural tetraploid T. pratense L.‘Elçi’could be realized only through the apical meristem calli. In order to proceed to the production stage, other regeneration methods need to be tried. Aseptic seedlings were used for the production of somatic embryos through various 2,4-D and kinetin trials. Nonuniform external callus cells with translucent cytoplasm were observed in various developmental stages of somatic embryos. Beneath these cells, there were uniformly aligned, dark-stained embryo cells with dense cytoplasm. Despite the similar developmental stages and cell characteristics of zygotic and somatic embryos, the walls of somatic embryo cells revealed a highly wavy pattern. The nucleus generally contained only one nucleolus, which was spherical, dark stained, and electron-dense. Electron-dense droplets were seen in vacuoles. The cytoplasm consisted of starch-containing amyloplasts, mitochondria, plastids, ribosomes, endoplasmic reticulum, dictyosomes, lipid, and protein bodies. In some of the somatic embryos at the globular and heart stages, vacuole or electron-translucent zones were observed in the nucleolus. Additionally, a few embryo degenerations were recorded during developmental stages of the zygotic embryo. For the first time, the somatic embryos of natural tetraploid T. pratense were produced from hypocotyl (85%), cotyledon (75%), and apical meristem (60%) explants in 0.3 mg/L 2,4-D and 2 mg/L kinetin-containing MS medium. Our study developed an effective and efficient in vitro production method for using natural tetraploid T. pratense in biotechnological studies Daha fazlası Daha az

Three remarkable new moss records for South-West Asia from northern Turkey

Uyar G. | Ören M.

Makale | 2013 | Turkish Journal of Botany37 ( 2 ) , pp.363 - 368

Grimmia anomala Schimp., Pohlia filum (Schimp.) Mårtensson, and Hookeria acutifolia Hook. & Grev. are noteworthy moss species reported here for the first time in South-West Asia from specimens collected in north-eastern and north-western Turkey. The diagnostic characters, habitat preferences, illustrations, and updated ranges of the treated species are given together with notes comparing them with those of related taxa. © TÜBİTAK.

Classification of Turkish Plantago L. species using numerical Taxonomy

Tutel, Betül | Kandemir, İrfan | Kuş, Semra | Kence, Aykut

Makale | 2005 | Turkish Journal of Botany29 ( 1 ) , pp.51 - 61

Bu çalışmada Türkiye'de dağılım gösteren Euplantago Harms ve Psyllium (Juss.) Harms altcinslerine ait 23 Plantago taksonu sayısal taksonomi yöntemleri kullanılarak sınıflandırılmıştır. Plantago (aksonlarının nümerik taksonomik analizi için toplam 36 morfolojik ve ekolojik karakter kullanılmıştır. Sayısal taksonomik yöntemler sonucunda elde edilen sınıflandırma geleneksel sınıflandırma ile karşılaştırılmıştır. Genelde iki sınıflandırma uyum göstermekle birlikte, Hymenopsyllium Pilger seksiyonu geleneksel sınıflandırmanın aksine Psyllium altgenusu ile kümelenmiştir. Twenty-three Plantago L taxa belonging to 2 subgenera (Euplantago Har . . .ms and Psyllium (Juss.) Harms) were classified by numerical taxonomic methods. A total of 36 morphological and ecological characters were utilised for numerical taxonomic analysis of Plantago taxa. The classification obtained by numeric taxonomic methods was compared to that obtained by conventional methods. In general, both of the classifications agreed well, except for the section Hymenopsyilium Pilger being clustered under the subgenus Psyllium contrary to the conventional classification Daha fazlası Daha az

The bryophyte flora of the western part of the Küre Mountains (Bartin, Kastamonu), Turkey

Ören M. | Uyar G. | Keçel T.

Makale | 2012 | Turkish Journal of Botany36 ( 5 ) , pp.538 - 557

Th is study presents the bryophyte flora of the western part of the Küre Mountains, situated in A2 square in the grid system adopted by Henderson, and entirely within the Euro-Siberian phytogeographic region. A total of 1545 bryophyte specimens were collected from the research area in 2008 and 2009. According to the results of this study, 272 taxa belonging to 146 genera and 60 families were recorded. Of these, 2 taxa are hornworts, 53 taxa are liverworts, and 217 taxa are mosses. Among them, 38 taxa are new records for A2 and 4 taxa [Leiocolea badensis (Gottsche) Jörg., Cephaloziella rubella (Ness) Warnst., Diphyscium foliosum (Hed . . .w.) D.Mohr, and Brachythecium tommasinii (Sendtn. ex Boulay) Ignatov & Huttunen] are recorded for the second time in Turkey. In addition, Seligeria trifaria (Brid.) Lindb. and Pseudotaxiphyllum elegans (Brid.) Z.Iwats are new records for the moss flora of Turkey. At the same time, Pseudotaxiphyllum Z.Iwats. is a new genus record for Turkey Daha fazlası Daha az

A new record for the flora of Turkey: Scorzonera ketzkhovelii Grossh. (Asteraceae)

Hamzaoglu E. | Aksoy A. | Martin E. | Pinar N.M. | Çölgeçen H.

Makale | 2010 | Turkish Journal of Botany34 ( 1 ) , pp.57 - 61

Scorzonera ketzkhovelii Grossh. (Asteraceae) was recently collected from Yusufeli, Artvin (north-east Anatolia) and it has been reported as a new species record for the flora of Turkey. It is described and illustrated. In addition, its karyological features and pollen morphology are given. © TÜBİTAK.


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