Büyükgüzel, Kemal | Büyükgüzel, Ender | Erdem, Meltem | Küçük, Ceyhun | Atılgan, Utku Can

Proceedings | 2016 | FEBS JOURNAL283 , pp.66 - 66

41st FEBS Congress on Molecular and Systems Biology for a Better Life -- SEP 03-08, 2016 -- Kusadasi, TURKEY WOS: 000383616900195

Büyükgüzel, Ender | Erdem, meltem | Tunaz, Hasan | Küçük, Ceyhun | Atılgan, Utku Can | Stanley, David | Büyükgüzel, Kemal

Article | 2017 | Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology204 , pp.121 - 128

We posed the hypothesis that inhibition of eicosanoid biosynthesis leads to increased lipid peroxidation in insects. Here we report that rearing the greater wax moth, Galleria mellonella, on media supplemented with selected inhibitors of eicosanoid biosynthesis throughout the larval, pupal and adult life led to major alterations in selected oxidative and antioxidative parameters of wax moth and its ectoparasitoid, Bracon hebetor. The highest dietary dexamethasone (Dex), esculetin (Esc) and phenidone (Phe) led to increased malondialdehyde (MDA) levels and to elevated catalase (CAT) and glutathione-S-transferase (GST) activities in al . . .l developmental stages of host larvae. Dietary Phe resulted in increased MDA levels, and CAT activity in G. mellonella adults by about 4-fold and about 2-fold, respectively. The Phe effect on GST activity in all stages of the wax moth was expressed in a dose-dependent manner, increased to 140 nmol/mg protein/min in larvae. MDA levels were increased by over 30-fold in adult wasps reared on Dex- and Esc-treated hosts. CAT and GST activities were increased in adult parasitoids reared on Esc-and Phe-treated hosts. GST activity of Dex-treated parasitoid larvae increased from about 4 to over 30 nmol/mg protein/min. Dietary Phe led to increased GST activity, by about 25-fold, in adult wasps. These data indicate that chronic inhibition of eicosanoid biosynthesis leads to increased oxidative stress, strongly supporting our hypothesis. The significance of this work lies in understanding the roles of eicosanoids in insect biology. Aside from other well-known eicosanoids actions, we propose that eicosanoids mediate reductions in oxidative stress. © 2016 Elsevier Inc Daha fazlası Daha az

Erdem, Meltem | Küçük, Ceyhun | Büyükgüzel, Ender | Büyükgüzel, Kemal

Article | 2016 | Archives of Insect Biochemistry and Physiology93 ( 4 ) , pp.202 - 209

Gemifloxacin mesylate (GEM) is a synthetic, fourth-generation fluoroquinolone antibacterial antibiotic that has a broad spectrum of activity against bacteria. GEM inhibits DNA synthesis by inhibiting DNA gyrase and topoisomerase IV activities. Recent research into insect nutrition and mass-rearing programs, in which antibiotics are incorporated into the culture media to maintain diet quality, raised a question of whether clinical antibiotics influence the health or biological performance of the insects that ingest these compounds. Because some antibiotics are pro-oxidant compounds, we addressed the question with experiments designed . . . to assess the effects of GEM (mesylate salt) on oxidative stress indicators, using Galleria mellonella larvae. The insects were reared from first-instar larvae to adulthood on artificial diets amended with GEM at 0.001, 0.01, 0.1, or 1.0%. Feeding on the 1% diets led to significantly increased hemolymph contents of the lipid peroxidation product, malondialdehyde and protein oxidation products, protein carbonyl. All GEM concentrations led to increased hemolymph glutathione S-transferase activity. We inferred that although it was not directly lethal to G. mellonella larvae, dietary exposure to GEM exerts measurable oxidative damage, possibly on insects generally. Long-term, multigenerational effects remain unknown. © 2016 Wiley Periodicals, Inc Daha fazlası Daha az

Büyükgüzel, Kemal | Coşkuncu, Kıymet Senan | Büyükgüzel, Ender | Erdem, Meltem | Küçük, Ceyhun

Article | 2017 | Karaelmas Fen ve Mühendislik Dergisi7 ( 1 ) , pp.341 - 348

Hymenopter endoparazitoid bir tür olan Pimpla turionellae (L). (Hymenoptera: Ichneumonidae) büyük bal mumu güvesi Galleria mellonella L.’nın de dahil olduğu bir çok lepidopter türün parazitoitidir. Yumurtalarını bu böceklerin puplarının içine bırakırlar. Yumurtalar gelişip açıldıktan sonra serbest kalan larvalar konak pupların hemolenfi ve dokuları ile beslenerek konakları öldürmektedir. Böylece bu böcekler zararlı böceklerle biyolojik mücadele programlarında önemli bir role sahiptir. Bu nedenle parasitoid P. turionellae’nın kitlesel olarak üretimi tarımsal ürünler kadar Zonguldak’taki orman ağaçlarının (meyve ağaçları ve endüstriye . . .l öneme sahip ağaçlar) zararlılardan korunması için oldukça önemlidir. Bu parazitoidin doğal konak G. mellonella’nin pupları kulanılarak laboratuvar şartlarında ve kimyasal yapısı bilinen sentetik besinler ile laboratuvar şartlarında kitle üretimi yapılmaktadır. P. turionellae laboratuvarda kitle halinde üretilerek gerektiği zaman zararlı böceklerin yoğun olduğu ormanlık alanlara bırakılarak ağaçların zararlılardan korunması sağalanabilir. Pimpla turionella L. (Hymenoptera: Ichneumonidae) is endoparasitoids of many species of lepidoptera, including greater wax moth Galleria mellonellae L. These parasitoids lay their eggs into pupae of these host insects. After developind and hatching of the eggs, the larvae feed on the haemolymph and tissues of the host pupae. The hosts are eventually killed by these developing larvae of P. turionellae. Therefore, these insects play an important role in biological control programmes. Therefore, mass-production of the parasitoids is very important to protect Zonguldak forest (including fruit and industrial trees) from pest insects as well as agricultural products. Using pupae of natural host G. mellonella and chemically synthetic diest for mass-production of this parasitoid is performed under laboratory conditions. After mass-rearing of P. turionellae (L.) in laboratory, they are released in the forest areas where especially pest insects are intensive Daha fazlası Daha az