The effects of boric acid-induced oxidative stress on antioxidant enzymes and survivorship in Galleria mellonella

Hyršl, Pavel | Büyükgüzel, Ender | Büyükgüzel, Kemal

Article | 2007 | Archives of Insect Biochemistry and Physiology66 ( 1 ) , pp.23 - 31

Larvae of the wax moth, Galleria mellonella (L.), were reared from first instar on a diet supplemented with 156, 620, 1,250, or 2,500 ppm boric acid (BA). The content of malondialdehyde (MDA, an oxidative stress indicator), and activities of the antioxidant enzymes [superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), and glutathione peroxidase (GPx)] were determined in the fat body and hemolymph in the 7th instar larvae and newly emerged pupae. Relative to control larvae, MDA was significantly increased in larval hemolymph, larval and pupal fat body, but decreased in the pupal hemolymph. Insects reared on di . . .ets with 156-and 620-ppm BA doses yielded increased SOD activity but 1,250- and 2,500-ppm doses resulted in decreased SOD activity in larval hemolymph. SOD activity was significantly increased but CAT was decreased in the larval fat body. High dietary BA treatments led to significantly decreased GST activity. However, they increased GPx activity in larval hemolymph. Dietary BA also affected larval survival. The 1,250- and 2,500-ppm concentrations led to significantly increased larval and pupal mortality and prolonged development. In contrast, the lowest BA concentration increased longevity and shortened development. We infer that BA toxicity is related, at least in part, to oxidative stress management. © 2007 Wiley-Liss, Inc Daha fazlası Daha az

The effects of oxyclozanide on survival, development and total protein of galleria mellonella L. (Lepidoptera: Pyralidae)

Çelik, Cihat | Büyükgüzel, Kemal | Büyükgüzel, Ender

Article | 2019 | Journal of the Entomological Research Society21 ( 1 ) , pp.95 - 108

We investigated that the effects of oxyclozanide on survival rate, development time, adult longevity and the amount of total protein (TP) in different developmental stages (7th-instars larvae, pupae and adult) of greater wax moth Galleria mellonella L. First-instar larvae of insects were reared on 0.003, 0.03 and 0.3 g oxyclozanide in 100 g artificial diets. When compared to all tested concentrations of oxyclozanide and control diet without oxyclozanide, it was confirmed that this anthelmintic drug significantly reduced survival rate of 7th-instar larval stage, pupal and adult stage of G. mellonella. While 7th-instars larval rate is . . . 91.25 ± 6.21 % in the control diet without oxyclozanide, this rate has been determined 28.75 ± 3.24 % in the 0.3 % concentration of oxyclozanide. TP of the insect increased in response to all concentration of oxyclozanide in comparison to control diet. This considerable increase was expressed almost two-fold especially at the 0.003 and 0.03 % of oxyclozanide concentrations in all developmental stages. The results obtained from this research support that the oxyclozanide has effects on the survival, development, adult longevity and TP of the insect. © 2019 Gazi Entomological Research Society. All rights reserved Daha fazlası Daha az

The influence of chronic eicosanoid biosynthesis inhibition on life history of the greater waxmoth, Galleria mellonella and its ectoparasitoid, Bracon hebetor

Büyükgüzel, Ender | Tunaz, Hasan | Stanley, David | Büyükgüzel, Kemal

Article | 2011 | Journal of Insect Physiology57 ( 4 ) , pp.501 - 507

Eicosanoids are oxygenated metabolites of three C20 polyunsaturated fatty acids, mainly arachidonic acid (AA; 20:4n-6), but also 20:3n-6 and 20:5n-3. Aside from their importance in biomedicine, eicosanoids act in invertebrate biology. Prostaglandins (PGs) influence salt and water transport physiology in insect rectal epithelia and in Malpighian tubules. PGs also influence a few insect behaviors, including releasing oviposition behavior and behavioral fever. Eicosanoids act in ovarian development and in insect immunity. Because eicosanoids act in several areas of insect biology, we posed the hypothesis that chronic inhibition of eico . . .sanoid biosynthesis, in the absence of microbial challenge, can influence insect life table parameters, including developmental time, survival, adult longevity and parasitoid fecundity. Here we report that inhibiting eicosanoid biosynthesis throughout the larval life exerted minor influences on some life table parameters of the greater wax moth, Galleria mellonella and its ectoparasitoid, Bracon hebetor, however, the inhibitors strongly reduced the production and hatchability of the parasitoids' eggs. The significance of the work relates to the potentials of understanding and targeting eicosanoid systems as a platform for developing new technologies of insect pest management. As seen here, the impact of targeting eicosanoid systems is seen in crucial moments of insect life histories, such as reproduction or immune challenge rather than in overall larval development. © 2011 Elsevier Ltd Daha fazlası Daha az

Reduced fitness in adults from larval, Galleria mellonella (Lepidoptera: Pyralidae) reared on media amended with the antihelmintic, mebendazole

Çalik, Gülşah | Büyükgüzel, Kemal | Büyükgüzel, Ender

Article | 2016 | Journal of Economic Entomology109 ( 1 ) , pp.182 - 187

Benzimidazole antihelmintics, including mebendazole, have a broad antiparasitic spectrum. These drugs play a major role in the treatments of parasites of intestines or other organs of vertebrates, humans, and other animals. The impact of mebendazole on the biology of the greater wax moth, Galleria mellonella (L.), was assessed by observation of several developmental parameters as follows: survivorship, developmental time, and adult longevity. Sublethal toxicity was measured through reproductive parameters such as fecundity and hatchability. The larvae were reared on artificial diet from first-instar larvae to the adult stage in the . . .laboratory. The diets contained mebendazole at different concentrations of 0.005, 0.05, 0.5, or 1.0%. Control diet did not contain mebendazole and produced seventh-instar larvae in 96.6±1.67% of cases, whereas the addition of mebendazole into diet at 1.0% significantly decreased survivorship of seventh-instar larvae to 79.9±4.08%. The diet with the highest concentration of mebendazole decreased survivorship in the adult stage from 79.9±2.35 to 56.6±4.73%, and shortened the developmental time for adult emergence from 36.7±0.48 to 34.1±0.63 d. All mebendazole concentrations shortened adult longevity and significantly decreased fecundity and hatchability of G. mellonella. The highest dietary concentration of this antihelmintic significantly decreased the egg number to 28.6±2.89 and hatching rate to 51.7±1.85%. The present study demonstrates that mebendazole exhibits significant adverse effects on greater wax moth, leading to deteriorated life table parameters and decreased adult fitness. © The Authors 2015 Daha fazlası Daha az

The effect of neomycin on survival and development of Pimpla turionellae L. (Hymenoptera: Ichneumonidae) reared on a natural host

Harmancı, Cumhur | Büyükgüzel, Kemal | Büyükgüzel, Ender

Article | 2019 | Journal of Economic Entomology112 ( 3 ) , pp.1081 - 1088

Understanding the effects of diet on metabolic events is crucial for biological control programs of parasitoid insects. As bioindicators of long-term physiological stress: survivorship of fifth instar larvae, pupation, adult survival, and developmental time for stages of endoparasitoid Pimpla turionellae L. (Hymenoptera: Ichneumonidae) were investigated by rearing the parasitoid on the host, Galleria mellonella L. (Lepidoptera: Pyralidae) pupae were treated with neomycin. First instar larvae of G. mellonella were reared on artificial diets containing 0.005, 0.01, or 0.5 g neomycin (g/100 g of diet) until seventh instar larvae; the p . . .upae from these larvae were used as a host for rearing P. turionellae. In the control group, the pupae from larvae reared on artificial diets without neomycin were used as a host. Survivorship of fifth instar, pupal, and adult stages of P. turionellae L. reared on G. mellonella pupae as a host fed with different concentrations of neomycin were significantly decreased in comparison to the control group. Approximately eighty percent of P. turionellae L. pupae were produced from control host pupae, while other neomycin concentrations significantly decreased the pupation of the parasitoid. Pimpla turionellae L. larvae reared on control host pupae reached fifth instar in about 9.6 ± 0.61 d, while the larvae reared from a host pupae exposed to the highest antibiotic concentration completed their development to the fifth instar in about 7.4 d. These results showed that neomycin, and possibly its metabolites, contaminated P. turionellae L. larvae from a host and affected larval stages of the parasitoid. © The Author(s) 2019. Published by Oxford University Press on behalf of Entomological Society of America Daha fazlası Daha az

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