Büyükgüzel, Kemal | Büyükgüzel, Ender | Erdem, Meltem | Küçük, Ceyhun | Atılgan, Utku Can

Proceedings | 2016 | FEBS JOURNAL283 , pp.66 - 66

41st FEBS Congress on Molecular and Systems Biology for a Better Life -- SEP 03-08, 2016 -- Kusadasi, TURKEY WOS: 000383616900195

Büyükgüzel, Ender | Büyükgüzel, Kemal

Article | 2016 | Journal of Economic Entomology109 ( 5 ) , pp.2090 - 2095

The effects of a synthetic purine nucleoside analog, antiviral agent, acyclovir (ACV), on adult longevity, fecundity, and hatchability of a serious honeycomb pest, greater wax moth Galleria mellonella L. were investigated by adding 0.01, 0.1, 1.0, and 3.0% ACV into artificial and natural diets. Control larvae were reared on diet without ACV. The artificial diet containing the lowest level of ACV, 0.01%, raised egg production from a number of 12.9 ± 0.6 to 163.2 ± 1.3. The hatching rate of these eggs was increased from 49.2 ± 2.4% to 68.2 ± 3.2%. Higher concentrations of ACV in natural food significantly increased both egg production . . . and egg hatching rate. Female reared on old dark combs as natural diet exposed to 1.0% of ACV produced 167.5 ± 5.8 eggs with 93.2 ± 6.8% hatched. This study emphasizes the importance of determining the dietary impact of an antimicrobial agent as a food additive to a particular species of insect before its using for dietary antimicrobial purpose. © 2016 The Authors Daha fazlası Daha az

Sugeçti, Serkan | Büyükgüzel, Ender | Büyükgüzel, Kemal

Article | 2016 | Journal of Entomological Science51 ( 2 ) , pp.129 - 137

Galleria mellonella L. larvae were reared on a standard diet amended with varying concentrations (0, 0.0015, 0.015, 0.15, and 1.5%) of the benzimidazole-derivative antihelmintic drug oxfendazole. Survivorship, developmental times, longevity, fecundity, and fertility were monitored over the treatments. Relative to the untreated control, exposure to diet containing 1.5% oxfendazole significantly decreased survivorship in larval, pupal, and adult stages, prolonged the time to reach the adult stage, and reduced adult longevity. Oxfendazole at all concentrations significantly lowered egg production per female and decreased egg hatch. The . . .se results demonstrate that this antihelmintic may be explored as a candidate for insect pest control Daha fazlası Daha az

Çalik, Gülşah | Büyükgüzel, Kemal | Büyükgüzel, Ender

Article | 2016 | Journal of Economic Entomology109 ( 1 ) , pp.182 - 187

Benzimidazole antihelmintics, including mebendazole, have a broad antiparasitic spectrum. These drugs play a major role in the treatments of parasites of intestines or other organs of vertebrates, humans, and other animals. The impact of mebendazole on the biology of the greater wax moth, Galleria mellonella (L.), was assessed by observation of several developmental parameters as follows: survivorship, developmental time, and adult longevity. Sublethal toxicity was measured through reproductive parameters such as fecundity and hatchability. The larvae were reared on artificial diet from first-instar larvae to the adult stage in the . . .laboratory. The diets contained mebendazole at different concentrations of 0.005, 0.05, 0.5, or 1.0%. Control diet did not contain mebendazole and produced seventh-instar larvae in 96.6±1.67% of cases, whereas the addition of mebendazole into diet at 1.0% significantly decreased survivorship of seventh-instar larvae to 79.9±4.08%. The diet with the highest concentration of mebendazole decreased survivorship in the adult stage from 79.9±2.35 to 56.6±4.73%, and shortened the developmental time for adult emergence from 36.7±0.48 to 34.1±0.63 d. All mebendazole concentrations shortened adult longevity and significantly decreased fecundity and hatchability of G. mellonella. The highest dietary concentration of this antihelmintic significantly decreased the egg number to 28.6±2.89 and hatching rate to 51.7±1.85%. The present study demonstrates that mebendazole exhibits significant adverse effects on greater wax moth, leading to deteriorated life table parameters and decreased adult fitness. © The Authors 2015 Daha fazlası Daha az

Erdem, Meltem | Küçük, Ceyhun | Büyükgüzel, Ender | Büyükgüzel, Kemal

Article | 2016 | Archives of Insect Biochemistry and Physiology93 ( 4 ) , pp.202 - 209

Gemifloxacin mesylate (GEM) is a synthetic, fourth-generation fluoroquinolone antibacterial antibiotic that has a broad spectrum of activity against bacteria. GEM inhibits DNA synthesis by inhibiting DNA gyrase and topoisomerase IV activities. Recent research into insect nutrition and mass-rearing programs, in which antibiotics are incorporated into the culture media to maintain diet quality, raised a question of whether clinical antibiotics influence the health or biological performance of the insects that ingest these compounds. Because some antibiotics are pro-oxidant compounds, we addressed the question with experiments designed . . . to assess the effects of GEM (mesylate salt) on oxidative stress indicators, using Galleria mellonella larvae. The insects were reared from first-instar larvae to adulthood on artificial diets amended with GEM at 0.001, 0.01, 0.1, or 1.0%. Feeding on the 1% diets led to significantly increased hemolymph contents of the lipid peroxidation product, malondialdehyde and protein oxidation products, protein carbonyl. All GEM concentrations led to increased hemolymph glutathione S-transferase activity. We inferred that although it was not directly lethal to G. mellonella larvae, dietary exposure to GEM exerts measurable oxidative damage, possibly on insects generally. Long-term, multigenerational effects remain unknown. © 2016 Wiley Periodicals, Inc Daha fazlası Daha az

Chowanski, Szymon | Adamski, Zbigniew | Marciniak, Pawel | Rosiński, Grzegorz | Büyükgüzel, Ender | Büyükgüzel, Kemal | Falabella, Patrizia | Scrano, Laura | Ventrella, Emanuela | Lelario, Filomena | Bufo, Sabino A.

Article | 2016 | Toxins8 ( 3 ) , pp.202 - 209

Only a small percentage of insect species are pests. However, pest species cause significant losses in agricultural and forest crops, and many are vectors of diseases. Currently, many scientists are focused on developing new tools to control insect populations, including secondary plant metabolites, e.g., alkaloids, glycoalkaloids, terpenoids, organic acids and alcohols, which show promise for use in plant protection. These compounds can affect insects at all levels of biological organization, but their action generally disturbs cellular and physiological processes, e.g., by altering redox balance, hormonal regulation, neuronal sign . . .alization or reproduction in exposed individuals. Secondary plant metabolites cause toxic effects that can be observed at both lethal and sublethal levels, but the most important effect is repellence. Plants from the Solanaceae family, which contains numerous economically and ecologically important species, produce various substances that affect insects belonging to most orders, particularly herbivorous insects and other pests. Many compounds possess insecticidal properties, but they are also classified as molluscides, acaricides, nematocides, fungicides and bactericides. In this paper, we present data on the sublethal and lethal toxicity caused by pure metabolites and crude extracts obtained from Solanaceae plants. Pure substances as well as water and/or alcohol extracts cause lethal and sublethal effects in insects, which is important from the economical point of view. We discuss the results of our study and their relevance to plant protection and management Daha fazlası Daha az

Ventrella, Emanuela | Adamski, Zbigniew | Chudzinska, Ewa | Miadowicz-Kobielska, Mariola | Marciniak, Pavel | Büyükgüzel, Ender | Büyükgüzel, Kemal | Erdem, Meltem | Falabella, Patrizia | Scrano, Laura | Bufo, Sabino Aurelio

Article | 2016 | PLoS ONE11 ( 5 ) , pp.202 - 209

Glycoalkaloids are secondary metabolites commonly found in Solanaceae plants. They have anti-bacterial, anti-fungal and insecticidal activities. In the present study we examine the effects of potato and tomato leaf extracts and their main components, the glycoalkaloids ?-solanine, ?-chaconine and ?-tomatine, on development and reproduction of Drosophila melanogaster wild-type flies at different stages. Parental generation was exposed to five different concentrations of tested substances. The effects were examined also on the next, non-exposed generation. In the first (exposed) generation, addition of each extract reduced the number . . .of organisms reaching the pupal and imaginal stages. Parent insects exposed to extracts and metabolites individually applied showed faster development. However, the effect was weaker in case of single metabolites than in case of exposure to extracts. An increase of developmental rate was also observed in the next, non-exposed generation. The imagoes of both generations exposed to extracts and pure metabolites showed some anomalies in body size and malformations, such as deformed wings and abdomens, smaller black abdominal zone. Our results further support the current idea that Solanaceae can be an impressive source of molecules, which could efficaciously be used in crop protection, as natural extract or in formulation of single pure metabolites in sustainable agriculture. © 2016 Ventrella et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Daha fazlası Daha az

Hız, Pınar | Erdem, Meltem | Büyükgüzel, Ender | Büyükgüzel, Kemal

Article | 2016 | KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI22 ( 5 ) , pp.777 - 784

Greater wax moth, Galleria mellonella L. is most commonly used as model insects for studying insecticidal efficiency of chemicals as well as it has been known as an alternative model to mamalian model for studying microbial infections and clinical drugs. The effects of a fluoroquinolone antibacterial agent, gemifloxacin, on sex ratio, male and female adult longevity, fecundity and hatchability of this insect were investigated by rearing the first instar larvae on artificial diets in the laboratory condition. The insect was reared from first-instar larvae to adult stage on an artificial diets containing gemifloxacin at 0.001, 0.01, 0 . . ..1 or 1.0%. Gemifloxacin did not affect male and female sex ratio and adult longevity while it significantly decreased egg number. Fecundity and hatchability were significantly and inversely decreased by increasing gemifloxacin concentrations. The females reared from control diet produced 134.46 per day, whereas high concentrations of gemifloxacin decreased the egg number to 26.75 and 53.5. Hatchability was also significantly decreased by 0.01% and above concentrations of gemifloxacin whereas, the highest concentration of antibiotic lowered the hatchability to 53.71%. This study indicated sublethal effects of gemifloxacin are likely to have a significant impact on adult biological traits and these effects show concentration-dependent variation in biological traits of the insect Daha fazlası Daha az

Erdem, Meltem | Büyükgüzel, Ender | Büyükgüzel, Kemal

Article | 2016 | Journal of Entomological Science51 ( 4 ) , pp.305 - 313

he fruit fly Drosophila melanogaster (Meigen) (Diptera: Drosophilidae) is often used in various biological, molecular, and toxicological studies. Sodium tetraborate, a boron compound, was added to the artificial diet of developing D. melanogaster to determine its effects on adult longevity and fecundity. Insects reared from first instars to adults on diets containing 10, 30, 150, 300, or 400 mg/liter sodium tetraborate showed that the highest concentration (400 mg/liter) significantly reduced female longevity (31.65 6 4.02 [mean 6 SD] d for the controls versus 1.87 6 0.30 d for 400 mg/liter) and male longevity (32.80 6 1.96 d for co . . .ntrols versus 3.57 6 0.42 d for 400 mg/liter). Females from the control diet produced 9.46 6 0.57 (mean 6 SD) eggs per female, whereas those fed on a diet containing 300 mg/liter produced only 1.92 6 0.30 eggs per females. These results expand our knowledge of the impact of sodium tetraborate on various insects and indicate that boron compounds should be further investigated to ascertain their potential as an alternative control tactic for pest insects Daha fazlası Daha az

Hız, Pınar | Erdem, Meltem | Büyükgüzel, Ender | Büyükgüzel, Kemal

Article | 2016 | Kafkas Üniversitesi Veteriner Fakültesi Dergisi22 ( 5 ) , pp.777 - 784

Büyük bal mumu güvesi Galleria mellonella L. mikrobiyal enfeksiyonların ve klinik ilaçların çalışılmasında memeli modellerine alternatif bir model olarak bilindiği gibi insektisit etkinliği çalışmalarında da model böcek olarak yaygın kullanıma sahiptir. Bu böceğin larvaları yapay besin ortamında beslenerek fluorokinolon sınıfından bir antibiyotik olan gemifloksasinin böceğin eşey oranı, dişi ve erkek ömür uzunluğu, yumurta verimi, açılma oranı gibi ergin biyolojik özellikleri üzerine etkisi laboratuvar şartlarında incelendi. Böceğin birinci evre larvaları %0.001, 0.01, 0.1 ve 1.0 oranında gemifloksasin içeren yapay besinler ile ergi . . .n evreye kadar beslendi. Gemifloksasinin denenen konsantrasyonlarını içeren besinler erkek ve dişi eşey oranı ile erginlerin ömür uzunluğu üzerinde etkili olmadığı halde yumurta verimini önemli derecede düşürdüğü belirlendi. Artan besinsel gemifloksasin konsantrasyonları ile ters orantılı olarak yumurta verimi ve açılma oranı azaldı. Kontrol besini ile yetiştirilen dişiler günde 134.46 adet yumurta üretirken gemifloksasinin yüksek miktarlarında dişiler 26.75 ve 53.5 yumurta bıraktılar. Yumurtaların açılma oranı da gemifloksasinin %0.01'lik konsantrasyonundan itibaren önemli derecede azalmış olup, en yüksek konsantrasyon bu oranı %53.71'e düşürdü. Bu çalışma erginlerin biyolojik özelliklerinin gemifloksasin tarafından önemli derecede etkilendiğini ve bu etkilerin antibiyotiğin konsantrasyonlarına bağımlı değişimler olduğunu gösterdi. Greater wax moth, Galleria mellonella L. is most commonly used as model insects for studying insecticidal efficiency of chemicals as well as it has been known as an alternative model to mamalian model for studying microbial infections and clinical drugs. The effects of a fluoroquinolone antibacterial agent, gemifloxacin, on sex ratio, male and female adult longevity, fecundity and hatchability of this insect were investigated by rearing the first instar larvae on artificial diets in the laboratory condition. The insect was reared from first-instar larvae to adult stage on an artificial diets containing gemifloxacin at 0.001, 0.01, 0.1 or 1.0%. Gemifloxacin did not affect male and female sex ratio and adult longevity while it significantly decreased egg number. Fecundity and hatchability were significantly and inversely decreased by increasing gemifloxacin concentrations. The females reared from control diet produced 134.46 per day, whereas high concentrations of gemifloxacin decreased the egg number to 26.75 and 53.5. Hatchability was also significantly decreased by 0.01% and above concentrations of gemifloxacin whereas, the highest concentration of antibiotic lowered the hatchability to 53.71%. This study indicated sublethal effects of gemifloxacin are likely to have a significant impact on adult biological traits and these effects show concentration-dependent variation in biological traits of the insect Daha fazlası Daha az