Ethyl pyruvate prevents from chronic cerebral hypoperfusion via preserving cognitive function and decreasing oxidative stress, caspase 3 activation and IL-1ß level

Özaçmak-Sayan, Hale | Özaçmak, Veysel Haktan | Turan, İnci

Article | 2018 | Bratislava Medical Journal119 ( 8 ) , pp.469 - 475

BACKGROUND: One of the important risk factors for dementia is chronic cerebral hypoperfusion (CCH) especially in patients with cerebrovascular disease. OBJECTIVES: In the present study, using rat model of bilateral common carotid artery occlusion, the possible protective effects of ethyl pyruvate (EP) have been explored in terms of memory impairment, oxidative stress, and levels of caspase-3, Na-K ATPase, and IL- 1ß. METHODS: Rats were treated with EP (50 mg/kg, i.p) for 4 weeks. Cognitive function was evaluated by Morris Water Maze (MWM). Both levels of caspase-3 and Na-K ATPase in tissue, IL-1ß in plasma were measured by ELISA met . . .hod. Status of oxidative stress in brain was assessed by the measurements of the tissue malondialdehyde (MDA) and reduced glutathione (GSH) contents. RESULTS: Results showed that CCH caused a striking impairment of spatial working memory, accompanied with increased levels of MDA and IL-1ß as well as caspase 3 level. The treatment with EP, however, significantly improved the memory impairment. Moreover, the treatment also provided beneficial effects on the disturbances of caspase 3, IL-1ß and MDA. CONCLUSION: This study strongly imply that the EP administration can alleviate the memory impairment observed due to CCH. The protection provided by EP may result from inhibition of inflammatory response, apoptotic processes and oxidative stress. © 2018, Comenius University Daha fazlası Daha az

Determination of candidate genes involved in schizophrenia using the whole-exome sequencing

Senormanci O. | Karatas Celik S. | Valipour E. | Dogan V. | Senormanci G.

Article | 2018 | Bratislava Medical Journal119 ( 9 ) , pp.572 - 576

OBJECTIVES: We used the whole-exome sequencing to evaluate several genes suspected of being involved in the pathogenesis of schizophrenia. METHODS: The study sample was composed of two families. In the first family, two siblings had schizophrenia, and the parents were healthy. In the second family, two siblings had schizophrenia, while the other sibling and the parents did not. RESULTS: Indels were detected in some genes, including SPON1, GRIA3, SMAD5, PCLO, KMT2C, SRD5A2, SEMA3B, NCOR2, GPHB5, FAM174B, CLTCL1, and TMEM216. The insertion of three nucleotides (TGA) was detected in the sequence of the PCLO gene. The mutation resulted . . .in the insertion of aspartic acid (Asp, D) in the amino acid sequence of the PCLO protein. Indels detected in SPON1, GRIA3, SMAD5, KMT2C, SRD5A2, SEMA3B, GPHB5, CLTCL1, and TMEM216 were shown to be frameshifting. Bioinformatics analysis showed that the indels in SPON1, GRIA3, SMAD5, KMT2C, SRD5A2, SEMA3B, NCOR2, GPHB5, FAM174B, CLTCL1, and TMEM216 had a damaging effect, while the indel in PCLO had a non-damaging effect on protein function. CONCLUSION: To the best of our knowledge, no previous studies have examined the relationship between the pathogenesis of schizophrenia and the gene mutations identified in this study. © 2018, Comenius University Daha fazlası Daha az

The role of kisspeptin on aromatase expression in breast cancer

Yilmaz M.B. | Oksuz H. | Ilgaz N.S. | Ocal I. | Tazehkand M.N.

Article | 2018 | Bratislava Medical Journal119 ( 12 ) , pp.776 - 780

AIM: Kisspeptin is a reproductive peptide hormone that has anti-metastatic roles in several cancer types including colon, lung, and brain cancer. However, in breast cancer, increasing of kisspeptin expression induces aggressiveness of tumors, which in turn exacerbates breast cancer prognosis. MATERIAL AND METHODS: Breast cancer cell lines MCF7 and SKBR3 were cultured in MEM (phenol red free) containing 10 % fetal bovine. Treatments were performed, at 70 % confluency, after 24-hour serum deprivation in serum free medium for 6, 24 and 48 hours. Aromatase (CYP19A1) and kisspeptin receptor (GPR54) mRNA expression were determined by real . . . time Taqman Assay. RESULT: Kisspeptin induced aromatase (CYP19A1) and kisspeptin receptor (GPR54) mRNA expression, while this induction was abolished by kisspeptin receptor inhibitor in MCF7 cells. In SKBR3 cells, however, even though there was an increase in GPR54 mRNA expression with kisspeptin, the induction of CYP19A1 was not observed. CONCLUSION: The inducing effect of kisspeptin on aromatase expression is possibly mediated via kisspeptin receptor and estrogen receptor dependent mechanisms. © AEPress s.r.o Daha fazlası Daha az

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