Özen O.A. | Songur A. | Sarsilmaz M. | Yaman M. | Kuş I.

Article | 2003 | Journal of Trace Elements in Medicine and Biology17 ( 3 ) , pp.207 - 209

Retrospective cohort studies and clinical findings have suggested effects of formaldehyde exposure on the central nervous system in anatomists, embalmers and pathologists. On the other hand, harmful effects of formaldehyde inhalation on the nervous system are not well documented. The concentrations of elements such as zinc, copper and iron within the cerebral cortex indicate whether physiological conditions are maintained. In this study, adult male albino Wistar rats were exposed to formaldehyde at different concentrations (0; 6.1; 12.2 mg·m-3) and during different periods of time (subacute-subchronic), and body weights were recorde . . .d weekly. Zinc, copper and iron concentrations were measured in the parietal cortex using atomic absorption spectrometry after wet ashing. We conclude that subacute or subchronic exposure to formaldehyde may cause growth retardation and alter zinc, copper and iron levels in the cerebral cortex Daha fazlası Daha az

Sarsilmaz M. | Songur A. | Özyurt H. | Kuş I. | Özen O.A. | Özyurt B. | Sögüt S.

Conference Object | 2003 | Prostaglandins Leukotrienes and Essential Fatty Acids69 ( 4 ) , pp.253 - 259

Omega-3 (?-3) is an essential fatty acid (EFA) found in large amounts in fish oil. It contains eicosapentaenoic acid and docosahexaenoic acid (DHA). DHA is one of the building structures of membrane phospholipids of brain and necessary for continuity of neuronal functions. Evidences support the hypothesis that schizophrenia may be the result of increased reactive oxygen species mediated neuronal injury. Recent reports also suggest the protective effect of ?-3 EFA against neuropsychiatric disorders including schizophrenia. This study proposed to assess the changes in antioxidant enzyme and oxidant parameters in the corpus striatum (C . . .S) of rats fed with ?-3 EFA diet (0.4 g/kg/day) for 30 days. Eight control rats and nine rats fed with ?-3 were decapitated under ether anesthesia, and CS was removed immediately. Thiobarbituric acid-reactive substances (TBARS) and nitric oxide (NO) levels as well as total superoxide dismutase (t-SOD) and xanthine oxidase (XO) enzyme activities in the CS were measured. Rats treated with ?-3 EFA had significantly lower values of TBARS ( Daha fazlası Daha az

Özen O.A. | Songur A. | Sarsilmaz M. | Yaman M. | Kuş I.

Article | 2003 | Journal of Trace Elements in Experimental Medicine16 ( 02.Mar ) , pp.67 - 74

The medical use of formaldehyde has focused especially on laboratory use. Harmful effects of formaldehyde inhalation, such as nasal squamous cell carcinoma, are quite well documented. However, detailed studies of the mechanisms of the damaging effects of formaldehyde on lung tissue are quite limited. Amounts of such elements as zinc, copper, and iron within lung tissue indicate whether or not they function physiologically. In this study, 36 adult albino Wistar male rats (6 animals per group) were exposed to 0 (Control), 5, and 10 ppm (1 ppm ? 1.2 mg/m3) of formaldehyde gas for subacute (4-week) and subchronic (13-week) periods. Whol . . .e-body exposure was continuously carried out for 8 h/day, 5 days/week. Body weights were recorded weekly. Levels of metals mentioned above were measured in lung tissue by atomic absorption spectrometry using wet ashing and were then compared with levels in the control groups. At the end of the study, a gradual decrease in weight gain, a decrease in zinc levels, and an increase in iron levels were observed in both subacute and subchronic groups compared to control groups. Statistically significant increases in tissue Fe levels were seen in the 10 ppm subacute (P < 0.001), 5 ppm subchronic (P < 0.001), and 10 ppm subchronic (P < 0.001) groups. We conclude that subacute or subchronic exposure to cytotoxic concentrations of formaldehyde may cause growth retardation and alter the trace element levels of zinc and iron in the lung tissue. © 2003 Wiley-Liss, Inc Daha fazlası Daha az

Songur A. | Akpolat N. | Kus I. | Ozen O.A. | Zararsiz I. | Sarsilmaz M.

Article | 2003 | Neuroscience Research Communications33 ( 3 ) , pp.168 - 178

The aim of this study was to investigate the effects of formaldehyde (FA) gas inhalation during the early postnatal period on the heat shock proteins 70 kDa (Hsp70) synthesis and morphological changes in the hippocampus in developmental process of rats and to determine whether the changes was reversible or not. Neonatal rats were exposed to 0 (control), 6 and 12 ppm FA gas throughout 30 day period following the birth. After the exposure to FA, some animals from each concentration group were decapitated at the day 30. Despite cessation of FA exposure, other groups were left for decapitation until the days of 60th and 90th. The brain . . .samples were stained with Hsp70 as immunohistochemically and H&E. In the samples, pyramidal cell layer in the hippocampus was examined. Hsp70 (+) neurons were found in the hippocampus of rats that inhaled FA on the 30th day. Furthermore, a considerable increase on the count of pyknotic neurons in these groups. But, on the other hand, in the 60th and 90th days, Hsp70 immunostaining and the count of pyknotic cells were found to be diminishing in FA inhaled groups. In conclusion, inhalation of FA gas in cytotoxic concentrations during early postnatal period causes increase in Hsp70 synthesis and damages the rat's hippocampus. Diminishment or disappearance of these negative changes in the 60th and 90th days indicates a reversible change in rats Daha fazlası Daha az

Kus I. | Akpolat N. | Oner H. | Ayar A. | Pekmez H. | Ozen O.A. | Sarsilmaz M.

Article | 2003 | Neuroendocrinology Letters24 ( 03.Apr ) , pp.209 - 214

Objective: The aim of this study was to examine the effects of photoperiod on testes in rat. Design: For this purpose 21 male Wistar rats were used. Animals were divided into three groups. Control rats in group I were kept under 12 hrs light: 12 hrs dark conditions (12L: 12D) for 10 weeks. Animals in group II were exposed to long photoperiods (18L: 6D), while rats in group III were exposed to short photoperiods (6L: 18D) for 10 weeks. At the end of the experiment, all animals were killed by decapitation and blood samples were obtained. Serum testosterone levels were determined with the use of a chemiluminescent enzyme immunoassay. T . . .he testes of all rats were removed and weighed. Testicular tissue was processed semiquantitative evaluation of immunohistochemical testosterone staining. Intensity of immunostaining was determined on a scale between 0 (no staining) and 5 (heavy staining). For morphometric comparison, diameters of seminiferous tubules in each group were measured. Results: In rats exposed to long photoperiods, testicular weights, diameters of seminiferous tubules and serum testosterone levels were significantly increased as compared to those in control rats. Whereas, exposure of rats to short photoperiods resulted in significantly decrease of testicular weights, diameters of seminiferous tubules and serum testosterone levels as compared to those in control rats and rats maintained in long photoperiods. Immunostaining of testosterone was moderate (3+) in control rats, heavy (5+) in rats exposed to long photoperiods and minimal (1+) in rats exposed to short photoperiods. Conclusions: The results of our study indicate that testicular functions increase after exposure to long photoperiods and decrease after exposure to short photoperiods Daha fazlası Daha az